BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,729,658	C→A	5.3%	A370S (GCA→TCA)	xylA ←	D‑xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,658	0	C	A	5.3%	349.3 / 5.0	114	A370S (GCA→TCA)	xylA	D‑xylose isomerase
Reads supporting (aligned to +/- strand): ref base C (51/56); new base A (3/4); total (54/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATC  >  NC_000913/3729510‑3729803

ggAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCa                                                                                                                                                  >  2:676343/1‑150 (MQ=255)

  agaCAAATGATGTACCTGAGCAAATTTGGCTAAATCTGCCAGTGACATATGGCCTTTCAGGATATGCTGCCCCAATTCGCTATTCCAG‑CCCGAATAACGCTGCGCGATGCGATTATCCAGCTCGCCATCTACAATCATGCGCGCTGCaaa                                                                                                                                                >  2:1033282/1‑149 (MQ=255)

   gaCAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAAtt                                                                                                                                               >  1:654190/1‑150 (MQ=255)

       aaTGATGTTGCTGAGCTTTTTTGGCTAAATCTGACAGTGAGATTTGGCCTTTCAGGATTTTGTGGGCCAATTCGCTATTCCAG‑CCGGAATATCGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTct                                                                                                                                           <  2:364088/150‑2 (MQ=255)

       aaTGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCa                                                                                                                                           <  1:633483/150‑1 (MQ=255)

         tgatgTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAgc                                                                                                                                         <  2:1427628/150‑1 (MQ=255)

         tgatgTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAgc                                                                                                                                         <  2:1434262/150‑1 (MQ=255)

            tgTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGcc                                                                                                                                      >  1:1452070/1‑150 (MQ=255)

              ttCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTTGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGAAAATTGCAgcgcgca                                                                                                                                    <  1:69359/150‑4 (MQ=255)

               tCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGAAAATTGCAgcgcgcat                                                                                                                                   <  1:137890/150‑5 (MQ=255)

               tCCTGAGCATATTTGACTAAATCTGCCAGTGACATTTGGCCTTTC‑GGATTTGCTTGCCCAATTGGCAATTACAGCCCGGAATAA‑GCATCGCGATGGGTTTATACCGGTGGCCATGATAAAAACTTCGCGGTTCAATTTTTAGCGCCAGtt                                                                                                                                  >  2:959026/1‑149 (MQ=255)

                ccTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTg                                                                                                                                  <  1:205535/150‑1 (MQ=255)

                ccTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTg                                                                                                                                  >  1:1095634/1‑150 (MQ=255)

                ccTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑ACGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGACAGTg                                                                                                                                  >  1:775769/1‑150 (MQ=255)

                ccTGAGCATAATTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTCTTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAAACATGCGCGCTGAAAATTGCAgcgcgcatg                                                                                                                                  <  2:81469/150‑6 (MQ=255)

                  tGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGcc                                                                                                                                >  1:563444/1‑150 (MQ=255)

                     gCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCCGCGCCAGTGCCATc                                                                                                                             >  2:265639/1‑150 (MQ=255)

                      cATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCg                                                                                                                            <  2:1662847/150‑1 (MQ=255)

                        tatTTGGCTAAATCTGCCAGTGACATTTGGCCTATCAGGATTTGCTGGCCAAATTCTCTATTCCAG‑CCGGAATAACGCTTCGCGATGCCTTTATACAGCTCGCCATCTTCAATCATGCGCTCTGAAAATTTCAGCGCCAGTGCCAACGTa                                                                                                                          >  1:732977/1‑150 (MQ=255)

                         atTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTAt                                                                                                                         <  1:1675633/150‑1 (MQ=255)

                          tttGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATc                                                                                                                        <  2:1289436/150‑1 (MQ=255)

                          tttGGCTAAATCTGCCAGTGACATTTGCCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTAACCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGtctc                                                                                                                        >  2:1289420/1‑150 (MQ=255)

                           ttGGCTTTATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑ACGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATcc                                                                                                                       <  2:100230/150‑1 (MQ=255)

                           ttGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATcc                                                                                                                       <  2:787583/150‑1 (MQ=255)

                               cTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATcg                                                                                                                   >  2:920118/1‑150 (MQ=255)

                                tAAATCTGCGAGTGACATTTGGCATTTCAGGATTTGCTGGCCGAATTCGCTATTCCAG‑GCGGTATAACGCTGCGCGTTGCGTTTATCCAGCTCGCCATCTTCTATCTTGCGCGCGGCAATTTTCAGCGCCAGTGCCATCGTATCCATcgc                                                                                                                  <  1:1056290/150‑1 (MQ=255)

                                   aTCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGcc                                                                                                               <  1:1291262/150‑1 (MQ=255)

                                     cTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGa                                                                                                             >  1:172787/1‑150 (MQ=255)

                                     cTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGa                                                                                                             >  2:793393/1‑150 (MQ=255)

                                     cTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCGCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGa                                                                                                             <  1:1635336/150‑1 (MQ=255)

                                           gtgtCATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGGTTTTGCTG‑CCGGAATATCGCTGCGCGATGCGTTTATCCAGCTCGCCTTCTTCAATCATGCGCGCTGCTATTTTCTGGGCCTGTGCCATCGTATCCATCGCGCCGATATGAc                                                                                                       <  1:1033282/150‑1 (MQ=255)

                                              aCATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGt                                                                                                    <  2:431930/150‑1 (MQ=255)

                                               cATTTGTCCTTTCAGGATTTGCTGGACCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGAAATTTTCAGAGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTa                                                                                                   <  2:232216/150‑1 (MQ=255)

                                               cATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTCCCATCGTATCCATCGCGCCGATATGACCGTa                                                                                                   >  1:1307197/1‑150 (MQ=255)

                                                 tttGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTaaa                                                                                                 >  1:1727474/1‑150 (MQ=255)

                                                  ttGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGCCCGTaaaa                                                                                                >  1:47471/1‑150 (MQ=255)

                                                       cTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGAt                                                                                           <  1:433400/150‑1 (MQ=255)

                                                       cTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGAt                                                                                           >  1:53685/1‑150 (MQ=255)

                                                        tttCATGATTTGCTGGGCCAATTCGCTATTCCAG‑GCGGAATTACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATc                                                                                          <  1:552347/150‑1 (MQ=255)

                                                        tttCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATc                                                                                          <  2:1724986/150‑1 (MQ=255)

                                                         ttCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCCTCGCGCCGATATGACCGTAAAACAGATCa                                                                                         <  2:898323/150‑1 (MQ=255)

                                                           cAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCata                                                                                       >  1:571754/1‑150 (MQ=255)

                                                            aGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCatat                                                                                      >  1:984707/1‑150 (MQ=255)

                                                            aGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCatat                                                                                      >  1:430522/1‑150 (MQ=255)

                                                              gATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATAttt                                                                                    <  2:1028217/150‑1 (MQ=255)

                                                               aTTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTa                                                                                   >  2:1652931/1‑150 (MQ=255)

                                                               aTTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTa                                                                                   <  2:1382622/150‑1 (MQ=255)

                                                                  tGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCa                                                                                <  1:712414/150‑1 (MQ=255)

                                                                    cTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGt                                                                              >  1:1593427/1‑150 (MQ=255)

                                                                    cTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCAGCGCGCCGATATGACCGTAAACCAGATCATATTTATCAGt                                                                              >  1:1217440/1‑150 (MQ=255)

                                                                      ggCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTAc                                                                            >  2:611428/1‑150 (MQ=255)

                                                                      ggCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTAc                                                                            >  1:1323458/1‑150 (MQ=255)

                                                                        cccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACa                                                                                              <  1:135218/130‑1 (MQ=255)

                                                                        cccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACa                                                                                              >  2:135218/1‑130 (MQ=255)

                                                                        cccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACtt                                                                          >  1:1638682/1‑150 (MQ=255)

                                                                        cccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACtt                                                                          <  2:53685/150‑1 (MQ=255)

                                                                        cccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTAGCCATCGCGCCGATATTACCGTAAAACAGATCTTATTTATCAGTACtt                                                                          >  1:1090193/1‑150 (MQ=255)

                                                                         ccAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGGTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACttt                                                                         <  1:1054879/150‑1 (MQ=255)

                                                                          cAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCGGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTg                                                                        <  2:971748/150‑1 (MQ=255)

                                                                               cGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTgacgac                                                                   <  2:909094/150‑1 (MQ=255)

                                                                               cGCTATTCAAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTgacgac                                                                   >  2:1524417/1‑150 (MQ=255)

                                                                                gCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGacgacg                                                                  <  2:23149/150‑1 (MQ=255)

                                                                                gCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGacgacg                                                                  <  1:793393/150‑1 (MQ=255)

                                                                                 cTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGt                                                                 >  1:1279844/1‑150 (MQ=255)

                                                                                   aTTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTAc                                                               <  2:1727474/150‑1 (MQ=255)

                                                                                      ccAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACttt                                                            >  2:372217/1‑150 (MQ=255)

                                                                                        aG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCGGCAATTTTCAGCGCCAGTGCCATCGTATGCATCGCGCCGATATGAACGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTgg                                                          >  2:835858/1‑150 (MQ=255)

                                                                                                   aCGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGtt                                                >  2:1564802/1‑150 (MQ=255)

                                                                                                    cGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTc                                               <  2:1570732/150‑1 (MQ=255)

                                                                                                     gCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCa                                              >  2:945968/1‑150 (MQ=255)

                                                                                                         ggcgATGCGTTTATCCAGCTCGCCATCTTCAATCTTGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGacc                                          <  2:1729389/149‑1 (MQ=255)

                                                                                                          gcgATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGacca                                         >  2:1366067/1‑150 (MQ=255)

                                                                                                              tGCGGTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCgg                                     <  1:1362516/150‑1 (MQ=255)

                                                                                                               gCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCggt                                    <  1:238737/150‑1 (MQ=255)

                                                                                                                cGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCTTCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCggtg                                   <  2:1133930/150‑1 (MQ=255)

                                                                                                                   ttATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGgtggtg                                <  2:1644085/150‑1 (MQ=255)

                                                                                                                   ttATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGgtggtg                                <  2:176056/150‑1 (MQ=255)

                                                                                                                   ttATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGgtggtg                                <  2:481969/150‑1 (MQ=255)

                                                                                                                     aTCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGCCGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGaa                              >  2:820609/1‑150 (MQ=255)

                                                                                                                      tCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGaaa                             >  1:1031511/1‑150 (MQ=255)

                                                                                                                       ccAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAAc                            <  1:829632/150‑1 (MQ=255)

                                                                                                                        cAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATTTGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCAACGGTGGTGAAAcc                           >  1:1685820/1‑150 (MQ=255)

                                                                                                                             cGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTg                      >  1:513861/1‑150 (MQ=255)

                                                                                                                             cGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTg                      >  1:921474/1‑150 (MQ=255)

                                                                                                                              gCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGc                     >  1:475134/1‑150 (MQ=255)

                                                                                                                               ccATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCt                    >  2:102187/1‑150 (MQ=255)

                                                                                                                                 aTCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCttt                  <  2:359726/150‑1 (MQ=255)

                                                                                                                                 aTCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCttt                  <  1:1564802/150‑1 (MQ=255)

                                                                                                                                  tCTTGAATCATGCGCGCTACAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAAGAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGAACTCCGGTTGTGAAACCGCCTGCTTTg                 <  2:850966/150‑1 (MQ=255)

                                                                                                                                     tCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTgaga              <  1:1531557/150‑1 (MQ=255)

                                                                                                                                      cAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGaa             >  2:780508/1‑150 (MQ=255)

                                                                                                                                      cAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGaa             <  1:1021217/150‑1 (MQ=255)

                                                                                                                                        aTCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGCCGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGaagt           >  1:144291/1‑148 (MQ=255)

                                                                                                                                         tCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCAGCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAAttt          <  2:1579004/150‑1 (MQ=255)

                                                                                                                                           actggcgcTGAAAATTGCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCa        >  1:1453577/5‑150 (MQ=255)

                                                                                                                                           actggcgcTGAAAATTGCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCa        >  1:1446817/5‑150 (MQ=255)

                                                                                                                                           aTGCGCGATGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCa        <  2:1617162/150‑1 (MQ=255)

                                                                                                                                            tGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCAGCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGAAGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCAt       <  2:1365229/150‑1 (MQ=255)

                                                                                                                                                cgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACAt   <  1:1524417/150‑1 (MQ=255)

                                                                                                                                                 gcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTTGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATc  <  1:265639/150‑1 (MQ=255)

GGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAG‑CCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATC  >  NC_000913/3729510‑3729803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`