| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,660,442 | C→G | 6.5% | L792V (CTG→GTG) | ynfE → | putative selenate reductase, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,660,442 | 0 | C | G | 6.5% | 335.4 / 12.5 | 107 | L792V (CTG→GTG) | ynfE | putative selenate reductase, periplasmic |
| Reads supporting (aligned to +/- strand): ref base C (52/48); new base G (2/5); total (54/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
AGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGC > NC_000913/1660294‑1660590 | aGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGCGCCTGGCACGAGGCCAATATGTCTGGCGATACAATCGACCATGGCGGCTGTGTGAATACGCGAACAACTCTGCGACCTTCACCACt > 1:1523605/1‑150 (MQ=255) gTTCGGCTACCAGCCAAAGTATCACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTg > 2:313303/1‑150 (MQ=255) gTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTg > 2:1392941/1‑150 (MQ=255) ggCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGa < 2:1209223/150‑1 (MQ=255) cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑g > 2:1619887/1‑150 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑gg < 1:1048587/150‑1 (MQ=255) aacAGCCAAAGTAACACCACGTTTTCTCCCTGGAGTTAGCGCTATGGTCCAGGGAGCCTGGCAGGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCTCTCTGCGCCCTTCACCACTGGCGAA‑ggg < 2:1429500/148‑1 (MQ=255) aCCATCCAAAGTCCCACCACGTATTATCCCTGGAGTTCGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCATTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑ggg < 1:250888/150‑1 (MQ=255) aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑ggg > 1:1316581/1‑150 (MQ=255) ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 1:1041791/150‑2 (MQ=255) ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 2:1701739/150‑2 (MQ=255) ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 2:28520/150‑2 (MQ=255) ccAGCAAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGACTGGCACGAGGCCAATATGTCTGGCTATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 2:1077444/150‑2 (MQ=255) aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGaaa > 1:141352/1‑150 (MQ=255) ccAAAGTAACACCTCGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAAcc < 1:1191290/150‑1 (MQ=255) cAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAAccc > 2:428204/1‑150 (MQ=255) aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTGGCGAA‑GGGAAGCCCgc > 1:1266339/1‑150 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCCCGAGGCCAATATGTCTGGCGCTAAAATCGACCATGGCTGCTGAGTGAATACTCTAACAACTCTGCGCCCATCACCACTGGCGAA‑GGTAAACCCgca > 2:311660/1‑150 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCgca < 1:848921/150‑1 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGCCTGTGTGAATACGCTACCCACTCTGCGCCCTTCCCCCCTGGCGAA‑GGGAAACCCgca > 2:502792/1‑150 (MQ=255) tAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCgcagc < 2:607369/150‑1 (MQ=255) aaCACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCgcagcc > 1:1302442/1‑149 (MQ=255) acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGcac < 1:512612/150‑1 (MQ=255) caccacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGcaca > 1:1572322/1‑150 (MQ=255) cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTa > 1:568652/1‑150 (MQ=255) aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTg > 2:864608/1‑150 (MQ=255) ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTc < 1:1000729/150‑1 (MQ=255) ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGTCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCgag < 1:39051/150‑1 (MQ=255) tGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGAt < 1:160779/150‑1 (MQ=255) ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAATATCGACGATGGCGGCTGTGTGAATACGCTTACCACTCTGCGCCCTTCACCGCTGGCGAA‑GGGATACCCGCAGCTCACTAATCTGGTCGAGATc > 1:1530084/1‑150 (MQ=255) gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 1:1077786/150‑1 (MQ=255) cTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat > 2:1012595/1‑150 (MQ=255) tATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAAtata < 2:933449/150‑1 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAattataa > 2:712917/1‑145 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 2:773931/1‑150 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:733237/1‑150 (MQ=255) ccAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc < 1:1432326/150‑1 (MQ=255) gggAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATAACCCACGAc > 2:444779/1‑150 (MQ=255) aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc > 1:61874/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTACCCACTCTGCGCCCTTCACCCCTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCa > 2:1581235/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa < 1:1084638/150‑1 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa < 1:499269/150‑1 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa > 1:551907/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCT‑GTCGAGATCGAAAAAATATAACCCACGACAACCAt > 2:1510522/1‑150 (MQ=255) ggCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGCTCGAAAAAATATAACCCACGACAACCATAAATTCTgg > 2:690146/1‑150 (MQ=255) gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc < 2:1692740/150‑1 (MQ=255) ccAATATGTCTGGCGATAAATTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGTA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGTTCGAAAAATTATTTCCGACGACACCCTTAATTTCTGGCa > 2:250591/1‑150 (MQ=255) ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCCCTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCCCGACAACCATAAATTCTGGCa > 1:94611/1‑150 (MQ=255) ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTTGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa < 1:297350/150‑1 (MQ=255) ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACAACCATAAATTcagtca > 1:236373/1‑145 (MQ=255) aaaTATGTCTGTCGATAATATCGACCATGTCGGATGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTTGCGAA‑GGGATACCCGCAGCACACTAATCTGGTCGAGATCGATAAAATATAACCCACGACAACCATAAATTCTGGCAt < 2:882945/149‑1 (MQ=255) aaTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATg > 2:1513262/1‑150 (MQ=255) tGTCTGGCTATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAt < 1:169582/150‑1 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt > 1:129481/1‑150 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTAACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt > 2:564595/1‑150 (MQ=255) tCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAttt > 2:1700278/1‑150 (MQ=255) cTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtttt < 2:733237/150‑1 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAATCCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATTTCCCACGACAACCTTAAATTCTGGCTTGACTGtttgt > 1:1049849/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 2:738515/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 2:771933/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 1:1023999/1‑150 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt < 2:737223/150‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 2:233110/150‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 2:1654035/150‑1 (MQ=255) aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa > 1:1314720/1‑150 (MQ=255) aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa > 1:179814/1‑150 (MQ=255) tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa < 1:104886/150‑1 (MQ=255) tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa < 1:28339/150‑1 (MQ=255) aaaaTCGACCATGGCGTCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGGCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa < 1:238624/150‑1 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGAAGCACACTAATCTGGTCTATATAGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc < 2:1316581/150‑1 (MQ=255) gACCATGGCGGCTGTGTGAATACTCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAAt < 1:325112/150‑1 (MQ=255) aTGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGt < 1:428204/150‑1 (MQ=255) tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg < 1:600859/150‑1 (MQ=255) ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa > 2:1147632/1‑150 (MQ=255) gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa > 1:418616/1‑150 (MQ=255) gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa > 1:1237471/1‑150 (MQ=255) tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatg < 2:111012/150‑1 (MQ=255) gAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaa > 1:66211/1‑150 (MQ=255) aTACGCTAACCACTCTGCGCCCTTCACCACTGGCGAC‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACCACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGataa > 1:1166523/1‑150 (MQ=255) aCGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATc < 1:212233/150‑1 (MQ=255) cGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATcc < 2:28150/150‑1 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAA‑CGGAAACCAGCAGCAAACTAATCTTGTCTAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCAt < 2:1133227/150‑1 (MQ=255) aaCCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc > 2:797957/1‑150 (MQ=255) aaCCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc < 2:962921/150‑1 (MQ=255) gggtttcccTTCGCCAGTGGTGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACaga > 2:525454/7‑150 (MQ=255) ctGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACagag < 1:565271/150‑1 (MQ=255) gcgcCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc > 1:1617341/1‑150 (MQ=255) tcccTTCGCCAGTGGTGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc > 2:1294689/2‑150 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCCGCTCACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCATAAATTCTGGCATGACATTTTGTTTGAATAGCAATAAGTGAGTAATGATGAAAATCCATACCCCAGAGGCGCTCa > 1:95639/1‑150 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa < 2:1061496/150‑1 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa > 1:1211438/1‑150 (MQ=255) ttCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt < 1:1669742/150‑1 (MQ=255) ttCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt < 2:916375/150‑1 (MQ=255) caccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATATGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa > 2:927241/1‑150 (MQ=255) caccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa > 2:1621841/1‑150 (MQ=255) ccacTGTCGAA‑GTGAAACCCGCAGCACACTAATCTGGTCGAGATCGAATAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAATAGCTATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg < 1:336653/150‑1 (MQ=255) ccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg > 2:1475426/1‑150 (MQ=255) ccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg > 1:1241428/1‑150 (MQ=255) acTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGc < 2:1083119/150‑1 (MQ=255) acTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGAGAGTAATGATGAAAATCCATACCACAGAGGCGATCATGAAGGc > 2:1408626/1‑150 (MQ=255) | AGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGC > NC_000913/1660294‑1660590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |