BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,618,945	A→T	5.6%	F406I (TTC→ATC)	uraA ←	uracil permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,945	0	A	T	5.6%	618.8 / 19.5	179	F406I (TTC→ATC)	uraA	uracil permease
Reads supporting (aligned to +/- strand): ref base A (77/92); new base T (2/8); total (79/100)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 184 total aligned reads displayed.

TCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTC  >  NC_000913/2618796‑2619093

tCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGaa                                                                                                                                                       <  1:4118823/150‑1 (MQ=255)

  acaAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGGGCAGCACACTGATCAGTTTGAAgt                                                                                                                                                     >  1:3854009/1‑149 (MQ=255)

  acaAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGa                                                                                                                                                     >  2:2912400/1‑150 (MQ=255)

  acaAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGa                                                                                                                                                     >  1:2037588/1‑150 (MQ=255)

  acaAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGa                                                                                                                                                     >  1:2037617/1‑150 (MQ=255)

   caAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGAt                                                                                                                                                    <  2:3740463/150‑1 (MQ=255)

    aaaaTCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATc                                                                                                                                                   <  1:924401/150‑1 (MQ=255)

     aaaTCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCa                                                                                                                                                  <  2:112569/150‑1 (MQ=255)

     aaaTCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCa                                                                                                                                                  <  2:1762176/150‑1 (MQ=255)

        tCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAAc                                                                                                                                               <  1:1347661/150‑1 (MQ=255)

            gagaAGTTTACCTCAGTATCCCATTATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCTCGTCTTCTGCGTCCATCTACACTTCTTCAGGGCGCAGCACACTGATCAGTTTGATCTTCAAACtgat                                                                                                                                           <  1:489410/150‑4 (MQ=255)

            gagaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATCTCCGCGTCTTCTGCGTCCAGCACCTCTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTaa                                                                                                                                           <  2:4129849/150‑1 (MQ=255)

            gagaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGATCTTCAAACtgat                                                                                                                                           <  1:83921/150‑4 (MQ=255)

            gagaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTaa                                                                                                                                           <  1:697897/150‑1 (MQ=255)

            gagaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTaa                                                                                                                                           <  1:2781960/150‑1 (MQ=255)

            gagaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTAGATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGATCTTCAAACtgat                                                                                                                                           <  1:1578509/150‑4 (MQ=255)

              gaAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGc                                                                                                                                         <  1:3510538/150‑1 (MQ=255)

                aGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCg                                                                                                                                       >  1:2138720/1‑150 (MQ=255)

                aGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCg                                                                                                                                       >  1:3792658/1‑150 (MQ=255)

                  tttACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGAt                                                                                                                                     >  1:1006623/1‑150 (MQ=255)

                   ttACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATa                                                                                                                                    <  1:3496340/150‑1 (MQ=255)

                   ttACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATa                                                                                                                                    <  2:2592377/150‑1 (MQ=255)

                       cacaGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcga                                                                                                                                <  2:2646748/150‑1 (MQ=255)

                         caGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacg                                                                                                                              >  2:1222243/1‑150 (MQ=255)

                         caGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacg                                                                                                                              >  2:1222189/1‑150 (MQ=255)

                          aGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                                                                                             <  2:1483962/150‑1 (MQ=255)

                          aGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                                                                                             <  2:1015500/150‑1 (MQ=255)

                          aGAAGCCCATGATGCTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTTTTTGACTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTACTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                                                                                             <  2:1714351/150‑1 (MQ=255)

                                 cATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGc                                                                                                                      >  1:2197550/1‑150 (MQ=255)

                                    gatgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTTTCTGTTATATAAGCGTCTTATGCGTACAGCACCACATCTTAAGGGCGCAGCACCAAGATAATTTTGAAGATAAAAATTAAGCCGATACCGAAAATGTTCGCCac                                                                                                                   >  1:354020/1‑149 (MQ=255)

                                      tgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcg                                                                                                                 <  2:1314312/150‑1 (MQ=255)

                                      tgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcg                                                                                                                 <  2:1852628/150‑1 (MQ=255)

                                       gTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcgc                                                                                                                >  1:2153312/1‑150 (MQ=255)

                                         ttGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCa                                                                                                              <  2:3321115/150‑1 (MQ=255)

                                         ttGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCa                                                                                                              <  1:3691841/150‑1 (MQ=255)

                                           gAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATa                                                                                                            <  2:3595799/150‑1 (MQ=255)

                                             aCCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATAcc                                                                                                          >  1:3018067/1‑150 (MQ=255)

                                                gggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑ttt                                                                                                      <  1:2451514/150‑1 (MQ=255)

                                                 ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑tttt                                                                                                     <  2:703318/150‑1 (MQ=255)

                                                 ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑tttt                                                                                                     <  1:1025133/150‑1 (MQ=255)

                                                  gcagcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGAGGGTCGCCAGCGCCATACC‑TTTTa                                                                                                    <  2:216005/150‑1 (MQ=255)

                                                  gcagcaACACTGCCCGGTCGGGACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTa                                                                                                    >  1:3584189/1‑150 (MQ=255)

                                                  gcagcaACACTGCCAGGTCGGTACCATATTTGTGTGTTATATCCGCGTATTCAGCGTCCAGCACCACTTCTTCAGGGCGCCGCACACTGATCAGTTTGAAGATAAAACTTAAGCCGATACCCACGATGGTAGCCAGCGCCAAACC‑Ttata                                                                                                    >  1:3584225/1‑150 (MQ=255)

                                                                      gtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGttt                                                                                >  1:3975829/1‑150 (MQ=255)

                                                                            tattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTc                                                                          >  1:1912946/1‑150 (MQ=255)

                                                                            tattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTc                                                                          <  2:3707372/150‑1 (MQ=255)

                                                                             attTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTcg                                                                         <  2:180612/150‑1 (MQ=255)

                                                                              cttGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTcgc                                                                        <  1:95381/149‑1 (MQ=255)

                                                                                tGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCgcgc                                                                      <  1:158594/150‑1 (MQ=255)

                                                                                tGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTGCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCgcgc                                                                      <  2:2853813/150‑1 (MQ=255)

                                                                                  tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCa                                                                    <  1:3344394/150‑1 (MQ=255)

                                                                                   cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCCGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCAc                                                                   <  1:1133470/150‑1 (MQ=255)

                                                                                    tGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACt                                                                  >  2:2505394/1‑150 (MQ=255)

                                                                                      ttATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGa                                                                <  2:3406219/150‑1 (MQ=255)

                                                                                      ttATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGa                                                                <  2:141700/150‑1 (MQ=255)

                                                                                      ttATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCAGATGTTAACCTACGCGCCACTGa                                                                >  2:1463700/1‑150 (MQ=255)

                                                                                       tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGAc                                                               <  2:2731129/150‑1 (MQ=255)

                                                                                                 tcttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATc                                                     <  2:3850278/150‑1 (MQ=255)

                                                                                                   ttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCaa                                                   >  1:3900579/1‑150 (MQ=255)

                                                                                                     ctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCaaaa                                                 >  1:2741053/1‑150 (MQ=255)

                                                                                                     ctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCaaaa                                                 >  2:1940320/1‑150 (MQ=255)

                                                                                                      tGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAAt                                                <  2:3062822/150‑1 (MQ=255)

                                                                                                      tGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAAt                                                >  1:3768965/1‑150 (MQ=255)

                                                                                                        cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCa                                              <  2:964276/150‑1 (MQ=255)

                                                                                                        cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCa                                              <  2:1021049/150‑1 (MQ=255)

                                                                                                             aGCACCACTTCTTCAGGGCGCAGCACTCTGATCTGTTTGTAGATCAAAGTTATGCCGTTAGCGACGATGGTCGCCAGCGCCATAGC‑TTTTATCTCCGCCGCGCCGTTGTTTTCCTTCGCGCCTCTGACGGCGATGTTCTTATTCTCGGaa                                         <  2:86254/150‑1 (MQ=255)

                                                                                                             aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCTCCAGCGCCATACCATTTAAACACCCCCGCCCCAATTTTAACGTACGCCCCACTGACCCCAAGAAGCAAAATCACGGa                                          >  1:2484300/1‑150 (MQ=255)

                                                                                                             aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGaa                                         >  1:2688114/1‑150 (MQ=255)

                                                                                                             aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGaa                                         >  1:2574449/1‑150 (MQ=255)

                                                                                                                accacTTCTTCCGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTc                                      <  1:2443773/150‑1 (MQ=255)

                                                                                                                 ccacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCa                                     <  2:2257140/150‑1 (MQ=255)

                                                                                                                 ccacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAAACACGGAAGTCa                                     >  2:3101924/1‑150 (MQ=255)

                                                                                                                  cacTTCTTCAGGGCGCAACACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                    >  2:391781/1‑150 (MQ=255)

                                                                                                                   acTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAgg                                   >  1:1207140/1‑150 (MQ=255)

                                                                                                                    cttcttCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGa                                  >  1:445222/1‑150 (MQ=255)

                                                                                                                     ttcttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTGTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGag                                 >  2:2396711/1‑149 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCAGGGAAGTCAGGATCAg                              >  2:730719/1‑150 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAg                              >  1:2512902/1‑150 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAg                              >  1:3528342/1‑150 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAg                              >  2:40732/1‑150 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAg                              >  1:3188374/1‑150 (MQ=255)

                                                                                                                        ttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAg                              >  2:106336/1‑150 (MQ=255)

                                                                                                                         tcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAgg                             >  2:3857133/1‑150 (MQ=255)

                                                                                                                         tcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCAGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAgg                             >  2:208552/1‑150 (MQ=255)

                                                                                                                               cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCtgt                       <  1:2369493/150‑1 (MQ=255)

                                                                                                                               cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCtgt                       <  1:1694359/150‑1 (MQ=255)

                                                                                                                               cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCtgt                       <  1:1694634/150‑1 (MQ=255)

                                                                                                                                gcagcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGAGGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCtgtg                      <  2:3284587/150‑1 (MQ=255)

                                                                                                                                 cagcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGc                     <  1:2780412/150‑1 (MQ=255)

                                                                                                                                 cagcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGc                     <  2:2700614/150‑1 (MQ=255)

                                                                                                                                   gcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                   >  1:1251180/1‑150 (MQ=255)

                                                                                                                                    cacaCTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCttt                  <  1:1501792/150‑1 (MQ=255)

                                                                                                                                       acTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgtt               >  2:1297960/1‑150 (MQ=255)

                                                                                                                                       acTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgtt               >  2:217563/1‑150 (MQ=255)

                                                                                                                                          cttaaGTTTGATCTTCAAACTTAAGCCGATACCGACGATGGTCGCAAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTa            >  2:4043879/5‑150 (MQ=255)

                                                                                                                                            tCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAAt          <  2:3404868/150‑1 (MQ=255)

                                                                                                                                                 ttGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACt     >  1:2966780/1‑150 (MQ=255)

                                                                                                                                                  tgtaGATCAAAGTTAAGCCGATACCGACGTTGGTCGCCTGCGCCATACG‑TTTTAACTGGGCCGCGCCGATGTTTACCTTCGCGCGACTGACGCCGATGATCAAATTCTGGGTTGTCAGGATCTGGTTCTGTGCTTTGTTGTATTCCAGtt    >  2:3097507/4‑150 (MQ=255)

                                                                                                                                                  tGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACtt    >  2:1665114/1‑150 (MQ=255)

                                                                                                                                                    aaGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTttc  <  2:3975829/150‑1 (MQ=255)

TCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACC‑TTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTC  >  NC_000913/2618796‑2619093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`