| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,648,452 | C→A | 8.5% | G1279V (GGT→GTT) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,648,452 | 0 | C | A | 8.5% | 347.7 / 15.4 | 121 | G1279V (GGT→GTT) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (54/56); new base A (4/7); total (58/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCA > NC_000913/2648303‑2648599 | cACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTAc < 1:472798/150‑1 (MQ=255) gCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTAcccc < 2:630848/150‑1 (MQ=255) gcgcACCCTGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGt < 2:684315/150‑1 (MQ=255) gcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTa < 1:1003851/150‑1 (MQ=255) gcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTa > 1:672450/1‑150 (MQ=255) cACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTaa < 1:884163/150‑1 (MQ=255) cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATg < 1:906559/150‑1 (MQ=255) cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAACGCAGCAGACGCTCATTACCCCGGTTAATg < 1:965041/150‑1 (MQ=255) cAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑Gc > 1:631247/1‑150 (MQ=255) gggAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGGTTTGAGATTTTCCGCGTACGGGATCGACATCATGCGCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTc < 1:1737585/149‑1 (MQ=255) cGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTc < 1:408502/150‑1 (MQ=255) cGGAGCCTGTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTc < 2:1547177/150‑1 (MQ=255) cGGAGACTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGAATGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTc < 1:1730562/150‑1 (MQ=255) gAGCCTTTTGCTGACTGGCCAACACCAGCGCAGCGTTAGACTGTACTGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCTTGCCCGGATCTTGTAAATAGCGCAGCAGACTCTCATTACCCCGGTTAATG‑GCGTCTg < 1:1826861/150‑1 (MQ=255) gAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTg < 2:1087621/150‑1 (MQ=255) gAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAA‑TTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc < 2:1014076/150‑2 (MQ=255) aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTTTCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc < 2:1327199/150‑2 (MQ=255) gCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgct < 1:1786271/150‑3 (MQ=255) gCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGGACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTc < 1:920771/150‑1 (MQ=255) ccTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGTCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCg < 2:384846/150‑1 (MQ=255) ccTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgctg < 2:1145668/150‑4 (MQ=255) cTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCgg < 1:1524155/150‑1 (MQ=255) gCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGc < 2:430117/150‑1 (MQ=255) cTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGACCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCt > 1:759890/1‑150 (MQ=255) tGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTg < 2:426541/150‑1 (MQ=255) gACGGGCCAACCCCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGt > 2:1537270/1‑150 (MQ=255) ggCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAAcc > 2:1558315/1‑150 (MQ=255) ccAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCt > 1:864914/1‑150 (MQ=255) ccAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCt < 2:1794190/150‑1 (MQ=255) acacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCAGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGtt > 2:1473652/1‑150 (MQ=255) cacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTc > 2:889567/1‑150 (MQ=255) ccAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGc > 2:25769/1‑150 (MQ=255) agcgcagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCt > 2:1291567/1‑150 (MQ=255) gcagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGCATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcg > 1:147578/1‑150 (MQ=255) cagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcgc < 1:1530592/150‑1 (MQ=255) agcgTAAGACTGTACTGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGcgcg < 2:751793/150‑1 (MQ=255) gcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCgg > 2:1716551/1‑150 (MQ=255) cgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGa > 2:27218/1‑150 (MQ=255) gTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGAc > 1:1685853/1‑150 (MQ=255) aGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAg < 1:1648229/150‑1 (MQ=255) aGACTGTACGGCGAATTTACTGGCTTTGAGATTATCAGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTAACCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCATGCGCGGACCAg > 1:1645024/1‑150 (MQ=255) aGACTGTACGGCGAATTTACTGGATTTGAGATTATCCGCGGACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAg < 2:1156444/150‑1 (MQ=255) gACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAgg > 1:1624239/1‑150 (MQ=255) gACTGTACGGCGAATTTACTGGCTTTGAGATTATCAGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGAAACCCATTTCGCCTCAGCCGACAAgg > 1:1624240/1‑150 (MQ=255) tGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGaaa < 2:486488/150‑1 (MQ=255) aCGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATcc < 2:969472/150‑1 (MQ=255) aCGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATcc > 2:971163/1‑150 (MQ=255) cGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCa > 1:763385/1‑150 (MQ=255) cGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCa < 2:1650840/150‑1 (MQ=255) ggCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTAGGCAAGCTGTAACACTGTTCGCCTGCGCGGACCAGGAAATCCAt > 1:770721/1‑150 (MQ=255) gCGAATTTACTGGCTTTGAGATTATCCGCGTCCGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATc < 1:1727638/150‑1 (MQ=255) aaTTTACTGGCTTTTAGAGTATCCGCGTACGGGATCGCCCTCATGCCCGGATCTTGCAACTCGCGCAGCAGACGCTCATTACCCCGGGTACGG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAAGCCATCTCg < 2:321065/150‑1 (MQ=255) tGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCg < 2:933927/150‑1 (MQ=255) ggCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGt > 1:1759308/1‑150 (MQ=255) tGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGcc < 2:759197/150‑1 (MQ=255) agaTTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAg > 2:1471773/1‑150 (MQ=255) gaTTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGCCGCTCATTACCCCGGTTTATG‑GCGTCGGTCGGCACGCTGTAACCCTGTTCGCGTGCGCGGACCAGGACAGCCATCACGTAAGCCGTCAGCCAGt > 1:1099671/1‑150 (MQ=255) ttATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTAc < 1:1436704/150‑1 (MQ=255) tATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGCTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTAct > 2:1517124/1‑150 (MQ=255) aTCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTActc > 1:1727925/1‑150 (MQ=255) ccGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCtt < 2:43086/150‑1 (MQ=255) gTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCAGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTc < 2:1581783/150‑1 (MQ=255) aCGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAc < 1:590465/150‑1 (MQ=255) aCGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAc > 1:850837/1‑150 (MQ=255) cGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAcc < 2:396356/150‑1 (MQ=255) ggATCGACATCATGCCCGGATCTTGTTAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAccct > 1:91003/1‑148 (MQ=255) ggATCGACATCATGCCCGGATCTTGTAAATAGCGCCGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGt > 1:86758/1‑150 (MQ=255) tCGACATCATGCCCGGATCTTGTAAATATCGCTGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACTCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGtttt < 1:788386/150‑1 (MQ=255) tCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCTGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGtttt < 2:864914/150‑1 (MQ=255) tCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGtttt > 2:1161397/1‑150 (MQ=255) gACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTa > 2:195465/1‑150 (MQ=255) aCATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCTCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTAt < 1:1133488/150‑1 (MQ=255) tcatGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATccc < 1:1473652/150‑1 (MQ=255) cccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgc > 1:1260832/1‑150 (MQ=255) cccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgc > 1:370819/1‑150 (MQ=255) cGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgcgc > 2:400848/1‑150 (MQ=255) cGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgcgc < 2:242966/150‑1 (MQ=255) cGGATCTTGTAAATAGCGCAGCAGACGCTCATTACACCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCAGCGCGGACCAGGAAATCCATCACGTAAGCCGTCATCCAGTACTCTTCGTCAACGTTTTTATACCACAgcgc > 2:773963/1‑150 (MQ=255) gATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCaa < 1:1422647/150‑1 (MQ=255) ttGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgcc < 1:1310647/150‑1 (MQ=255) gTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgccgc > 1:161622/1‑150 (MQ=255) aaaTAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGCCCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCGTCGTCACCGGTTTTATCCCACAGCGCAAAGccgccg > 1:1233483/1‑150 (MQ=255) aaaTAGCCCAGCAGACGCGCATTACCCCTGATAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCGCCGCGGACCAGGAAAACCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTGTTATCCCACAGCGCAAAGccgccg < 1:1396221/150‑1 (MQ=255) tAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTa > 2:370275/1‑150 (MQ=255) tAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTa < 1:1209703/150‑1 (MQ=255) gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc > 1:118671/1‑150 (MQ=255) cgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACATCGCAAAGCCGCCGTTATCa > 2:1314434/1‑150 (MQ=255) cgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCa > 1:825637/1‑150 (MQ=255) cgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATAACGTAAGCCGTCCGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATcc > 2:1494083/1‑149 (MQ=255) cgacagACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACg > 1:1137971/4‑150 (MQ=255) gcagACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGtt < 1:1558315/150‑1 (MQ=255) agACGCTCATTACCCCGGGTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGGCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:1648959/150‑1 (MQ=255) agACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc > 2:587608/1‑150 (MQ=255) gacgccATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCa > 2:1250678/6‑150 (MQ=255) cGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCAtt > 2:1405929/1‑150 (MQ=255) tCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgc < 2:1267310/150‑1 (MQ=255) cATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgca < 2:584312/150‑1 (MQ=255) cATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgca < 1:535469/150‑1 (MQ=255) ttACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgcagc > 1:495545/1‑150 (MQ=255) tACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgcagca < 1:25769/150‑1 (MQ=255) | CACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCA > NC_000913/2648303‑2648599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |