BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,648,129	C→T	5.1%	noncoding (67/84 nt)	agrA →	inactive antisense sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,129	0	C	T	5.1%	297.1 / 3.5	99	noncoding (67/84 nt)	agrA	inactive antisense sRNA
Reads supporting (aligned to +/- strand): ref base C (35/58); new base T (3/2); total (39/60)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 105 total aligned reads displayed.

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647980‑3648278

aaCTGGATAATCATACAGTACATGCATGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                       <  1:451825/150‑1 (MQ=255)

aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                       >  2:570976/1‑150 (MQ=255)

  atGGATAATCATACAGTACATGCTGGTTATAAAACCAGCACGTACTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCaa                                                                                                                                                     <  1:49363/149‑1 (MQ=255)

     gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                                  >  1:1697679/1‑150 (MQ=255)

         aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGCTGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                              >  2:251783/1‑150 (MQ=255)

           aaTACTGTAAATGCAGGTTATAAAACCAGCACGTCCTTGCAATTGTTTCAGTATGGTATTAGAATTGATGCGTTAGATTATGTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                            <  2:143412/149‑1 (MQ=37)

             tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttt                                                                                                                                          <  1:888473/150‑1 (MQ=255)

              aCAGTACATGCAGGTTATAAAACCAGCACTTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  1:310082/150‑1 (MQ=255)

              aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         >  2:1251130/1‑150 (MQ=255)

               cAGTACATGCAGGTTATAACACCTGCTCGTCCTTGCAATAGGTTCAGTATGGTATTAGCATTGATGCGTTATATGAGGGCTATCTCACTCCAGTCAGACCCACCAACTCAGGGCTGGAAAGT‑AAAATACCGTCGCAAAGTCGGttttttt                                                                                                                                        <  2:276670/150‑1 (MQ=25)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  2:1607669/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  1:1211584/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTAAGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  2:569126/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACAAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCTTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAACCCGACGCAAAGTCGGttttttt                                                                                                                                        <  1:852103/150‑1 (MQ=255)

               aaGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                         <  2:862042/149‑1 (MQ=38)

                aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACTAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       >  1:1341082/1‑150 (MQ=255)

                 gTACATGCAGTTTATAAAACCAGCAAGTACTTTCAATAGTTTCAGTATTGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                      <  2:69688/150‑1 (MQ=25)

                  tACATGCAGGTTATAAAACCAGCACGTCCTTGCTATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttttactt                                                                                                                                     <  1:1282401/150‑5 (MQ=37)

                  tACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACa                                                                                                                                     <  1:1606891/150‑1 (MQ=255)

                    cgtGCTGGTTTTATAACCTGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACTGGAAAGT‑AAAAACCCGAAGCAAAATAGGTTTTATTACAta                                                                                                                                   >  2:808221/3‑149 (MQ=255)

                    cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAGCTCGGGGGTGGAAAGT‑TAAAAACAGCAGAAAAGTCGGGATTTTTACagc                                                                                                                                   >  1:1026498/1‑148 (MQ=255)

                    cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTATCAGTATGGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCAACAAACTCAGGGCTGGAAAGT‑AAAAAACCGCCGCATAGTCGGTTTTTTTACATc                                                                                                                                   >  1:1196791/1‑150 (MQ=37)

                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                 >  1:1465186/1‑150 (MQ=255)

                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                 >  1:767075/1‑150 (MQ=255)

                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTAAGAGCCACAAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATcag                                                                                                                                 >  1:727239/1‑148 (MQ=255)

                          ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                             >  2:1211149/1‑150 (MQ=255)

                            ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                           <  1:64004/150‑1 (MQ=255)

                            ttATAAAACCAGCACGTCCTTGCAATACTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                           <  2:1370863/150‑1 (MQ=255)

                             tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTctg                                                                                                                          >  1:1407043/1‑148 (MQ=255)

                              ataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATATCACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGT‑AAAAAAACGACGCAAACTCGGTTTTTTTACATACTGATTccgc                                                                                                                         >  2:520007/1‑147 (MQ=37)

                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                       >  2:1135494/1‑150 (MQ=255)

                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                       >  2:760353/1‑150 (MQ=255)

                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                       >  2:610240/1‑150 (MQ=255)

                                  aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                     <  1:697159/150‑1 (MQ=255)

                                    ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGc                                                                                                                   <  2:1635491/150‑1 (MQ=255)

                                    ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACAGCAAAGT‑AAAAAACAGAAGCGAATTAGGGTATTTTACATCAGGAATCGGACAAGGc                                                                                                                   >  2:1514595/1‑150 (MQ=255)

                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                             <  2:1811707/150‑1 (MQ=255)

                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                             >  2:839766/1‑150 (MQ=255)

                                               ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACg                                                                                                        >  1:1264110/1‑150 (MQ=255)

                                               ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACg                                                                                                        <  2:221012/150‑1 (MQ=255)

                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                     <  2:677708/150‑1 (MQ=255)

                                                      aGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAccc                                                                                                 <  2:1780805/150‑1 (MQ=255)

                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                         >  1:1316210/1‑150 (MQ=255)

                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                         <  1:615022/150‑1 (MQ=255)

                                                                        gCATTGATGCGTTAGATGATTGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                               <  1:774355/150‑1 (MQ=255)

                                                                         cATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                              <  2:942839/150‑1 (MQ=255)

                                                                           ttGATGCGTTTGATGCTGGCTAGCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTc                                                                            <  2:1603263/150‑1 (MQ=255)

                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                        <  1:570976/150‑1 (MQ=255)

                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                        <  1:820571/150‑1 (MQ=255)

                                                                                  gTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGa                                                                     <  1:610240/150‑1 (MQ=255)

                                                                                          atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATTTAAATCGTCACTGCGATATATAcc                                                             <  2:1456700/150‑1 (MQ=255)

                                                                                           tgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCg                                                            >  2:1770981/1‑150 (MQ=255)

                                                                                                aTCTCACTCCAGTCATATCCACCAACTCATGGCTTGAAAGT‑AAAAAACCGACGCTAAGTCGGTTTTTTTTCATCCGGATTCGGACAAGGCTTAATATGATGATGACCCAGTGAATGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                       <  2:284137/150‑1 (MQ=37)

                                                                                                  ctcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGG‑TTTTCTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                    <  2:1697679/150‑1 (MQ=255)

                                                                                                   tctcTCCAGTCAGAGCCACCCACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATACGGCCAAGCCTTAATATGACGATGACCCAGTGAAACTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                    <  2:1196791/147‑1 (MQ=37)

                                                                                                   tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                    <  1:1178493/150‑1 (MQ=255)

                                                                                                   tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                    <  1:219305/150‑1 (MQ=255)

                                                                                                        ccAGTCAGATACACCAACTCAGGGATGGAAAGT‑AAAAAACCGACGCTAAGTCTGTTTTTTTACATCCTGATTCGCACAAGGCTTAATATGACGATGACCCAGTGAAAGTATAGAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                               <  1:281395/150‑1 (MQ=37)

                                                                                                         cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                              <  2:460190/150‑1 (MQ=255)

                                                                                                           gTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                            <  1:839766/150‑1 (MQ=255)

                                                                                                           ctCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                            <  2:478595/149‑1 (MQ=255)

                                                                                                            tCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCa                                           <  2:1008221/150‑1 (MQ=255)

                                                                                                              agagCCACCAACTCAGGGCAGGAAAGT‑AAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGAAAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGc                                         <  2:702076/150‑1 (MQ=255)

                                                                                                                 gCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTga                                      >  2:322153/1‑150 (MQ=255)

                                                                                                                   caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                    <  2:1741390/150‑1 (MQ=255)

                                                                                                                    accaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaaga                                   <  1:405075/150‑1 (MQ=255)

                                                                                                                         cTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                              <  1:1218678/150‑1 (MQ=255)

                                                                                                                          tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAATTCGGTTTTTTTACTTCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                             <  2:1630219/150‑1 (MQ=255)

                                                                                                                          tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                             <  2:1341082/150‑1 (MQ=255)

                                                                                                                           cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                            <  2:577012/150‑1 (MQ=255)

                                                                                                                           cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                            <  1:1502854/150‑1 (MQ=255)

                                                                                                                            aGGGCTGGAAAGT‑AAAAAAACGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGATATCGCTa                           <  1:1695056/150‑1 (MQ=255)

                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAATGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                        <  2:721221/150‑1 (MQ=255)

                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                        >  2:932839/1‑150 (MQ=255)

                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                        <  1:1135494/150‑1 (MQ=255)

                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                        >  2:1377074/1‑150 (MQ=255)

                                                                                                                                cTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtta                       >  2:803206/1‑149 (MQ=255)

                                                                                                                                cTGGAAAGA‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTc                       <  1:878508/150‑1 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                     >  1:1654531/1‑150 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                     >  2:721461/1‑150 (MQ=255)

                                                                                                                                   gAACGT‑AAAAAACCGAAGCAAAGTAGCTTTTTTTACATCACGATTAGGACAAGGCTTAATATGACGATGACCCACGGAAAGTATATAAATCTTCACTGCTAAATATAACGAAGTGCTCCCTCCTCCAGCTGAAGCAATCGCTAATTCGTg                    >  2:106715/1‑150 (MQ=255)

                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                  <  2:546295/150‑1 (MQ=255)

                                                                                                                                       gtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGCCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATAGTCACTGCGATATATACCGAAGTGCTCACTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                 >  2:1488651/4‑150 (MQ=255)

                                                                                                                                       gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                >  2:1805145/1‑150 (MQ=255)

                                                                                                                                       gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                <  1:760353/150‑1 (MQ=255)

                                                                                                                                        taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                >  2:1296631/3‑150 (MQ=255)

                                                                                                                                        t‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGTCGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg               <  2:1211893/150‑1 (MQ=255)

                                                                                                                                          aaaaaaCCGACTTTGCGTCGGTTTTTTTACATCATGATTCGGACAAGGCTAAATATGACGATGACCCAGTGAAAGTATATAAATCGTAACAGCGATATATACCGAAGTTCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGAAATGt              >  1:538630/1‑150 (MQ=255)

                                                                                                                                           aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt             >  2:581845/1‑150 (MQ=255)

                                                                                                                                           aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt             <  2:1142091/150‑1 (MQ=255)

                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCTTCACTGCGATATATAACGAAGTGCTCCCTCCGCCAGCTGAAGAACTCGCTAATTCTTGCAATGTTAGc          >  1:526699/1‑150 (MQ=255)

                                                                                                                                               aCCGCCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATAAACCGAAGTGCTCCCTCAGCCAGCTGAAGAAATCGCTAATTCTTGCCATGTTAGcc         >  1:475509/1‑150 (MQ=255)

                                                                                                                                                ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCa        >  1:1431300/1‑150 (MQ=255)

                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    >  2:1583091/1‑150 (MQ=255)

                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    >  2:1594765/1‑150 (MQ=255)

                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    <  1:1410448/150‑1 (MQ=255)

                                                                                                                                                     gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGc   <  1:251783/150‑1 (MQ=255)

                                                                                                                                                      cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  >  1:1634478/1‑150 (MQ=255)

                                                                                                                                                      cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  <  2:1264110/150‑1 (MQ=255)

                                                                                                                                                      cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  <  1:663701/150‑1 (MQ=255)

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647980‑3648278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`