BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	780,841:1	+T	7.0%	intergenic (+1/‑2)	valT → / → lysW	tRNA‑Val/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,841	1	.	T	7.0%	752.7 / 51.5	198	intergenic (+1/‑2)	valT/lysW	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base . (66/118); new base T (6/6); total (74/124)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

Only 100 of 199 total aligned reads displayed.

CGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGC  >  NC_000913/780695‑780984

cGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAATGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑Cgg                                                                                                                                              >  2:225674/1‑150 (MQ=255)

cGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑Cgg                                                                                                                                              <  1:847759/150‑1 (MQ=255)

  agaCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccactcggg                                                                                                                                             >  1:1017580/1‑145 (MQ=255)

   gaCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATCGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACCAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTc                                                                                                                                           <  2:1595083/150‑1 (MQ=255)

     cGAAGTCGAGTCATCCTGCACGACCCACCACTACCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGt                                                                                                                                         <  2:1795249/150‑1 (MQ=255)

     cGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGt                                                                                                                                         >  1:2042024/1‑150 (MQ=255)

       aaGTCGAGTCATCCTGCACGACCCACCACTCCCATAGTTAGTTGTAGTATCCAGCGTTGTATCGGGTGATTAGCTCAGCTGGGAGAGCTCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATGCCGTCATCACCCACCAC‑CGGGTCGTTa                                                                                                                                       <  2:1697752/150‑1 (MQ=18)

       aaGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTa                                                                                                                                       <  1:3708753/150‑1 (MQ=255)

        aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAg                                                                                                                                      >  1:3507617/1‑150 (MQ=255)

        aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAg                                                                                                                                      >  1:3325738/1‑150 (MQ=255)

        aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAg                                                                                                                                      <  1:2700569/150‑1 (MQ=255)

        aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAg                                                                                                                                      >  2:3836917/1‑150 (MQ=255)

         gTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGTGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGc                                                                                                                                     >  1:3652111/1‑150 (MQ=255)

            gAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTcc                                                                                                                                  >  2:1622507/1‑149 (MQ=255)

            gAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCa                                                                                                                                  >  2:4011196/1‑150 (MQ=255)

             aGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAg                                                                                                                                 >  2:1663353/1‑150 (MQ=255)

               tCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGtt                                                                                                                               <  1:2296364/150‑1 (MQ=255)

                 aTCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTgg                                                                                                                             <  1:2903375/150‑1 (MQ=255)

                   ccTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGt                                                                                                                            >  2:3029957/1‑150 (MQ=255)

                     tGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTaga                                                                                                                         <  2:3249877/150‑1 (MQ=255)

                     tGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTaga                                                                                                                         <  2:2617444/150‑1 (MQ=255)

                     tGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTaga                                                                                                                         <  1:11785/150‑1 (MQ=255)

                      gCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTagag                                                                                                                        <  2:3495158/150‑1 (MQ=255)

                       cACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGc                                                                                                                       <  1:2934789/150‑1 (MQ=255)

                        aCGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGTGTGTTTAGCTCAGCTGTGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                      <  2:1868767/150‑1 (MQ=255)

                          gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                                                                                     <  1:1312525/150‑1 (MQ=255)

                          gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                    <  1:884605/150‑1 (MQ=255)

                          gACCCACCACTAACAGAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                    <  2:722120/150‑1 (MQ=255)

                             ccaccaCTAACCTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGt                                                                                                                                         >  1:973980/1‑127 (MQ=255)

                             ccaccaCTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGt                                                                                                                                         <  2:973980/127‑1 (MQ=255)

                             ccaccaCTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa                                                                                                                 >  2:2928837/1‑150 (MQ=255)

                              caccacTACCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                                                                                <  2:173166/150‑1 (MQ=255)

                               accacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                                                                                                               >  2:2670428/1‑150 (MQ=255)

                                ccacTAACCTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCGGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                              <  2:3634696/150‑1 (MQ=255)

                                ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACACGGGACTTAAGAAAAGTTGGAAGACCAGtttata                                                                                                               >  2:2635821/1‑146 (MQ=14)

                                ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                              <  1:2452063/150‑1 (MQ=255)

                                 cacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                             <  1:3943734/150‑1 (MQ=255)

                                 cacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                             <  1:2858067/150‑1 (MQ=255)

                                    tAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                          <  2:804874/150‑1 (MQ=255)

                                    tAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATGAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                          <  2:1353033/150‑1 (MQ=255)

                                     aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                         >  1:4163160/1‑150 (MQ=255)

                                     aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                         >  1:2361263/1‑150 (MQ=255)

                                        aTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                      <  1:390751/150‑1 (MQ=255)

                                        aTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                      <  1:3188991/150‑1 (MQ=255)

                                         tagtttgtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                      <  1:3029957/150‑1 (MQ=2)

                                         tagttagtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                      <  2:2479475/150‑1 (MQ=17)

                                         tagttagtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                     <  2:1462292/150‑1 (MQ=255)

                                         tagttagtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                     <  2:1462296/150‑1 (MQ=255)

                                         tagttagtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                     <  2:747306/150‑1 (MQ=255)

                                          agttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                    <  2:489841/150‑1 (MQ=40)

                                              agttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCTCCTCCCTTACCAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                                                                                <  2:189509/150‑1 (MQ=255)

                                                ttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                                                                              >  1:1053868/1‑150 (MQ=35)

                                                  gtagtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                                                            <  2:4126534/150‑1 (MQ=35)

                                                  gtagtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                                                            <  2:308104/150‑1 (MQ=35)

                                                      taTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                        >  1:3799001/1‑150 (MQ=35)

                                                       aTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACGCGGGGCGTACGCGCAGTGGGTCGGGCAGTTGAATTTTAAGCAATTGGTCGCAGGtac                                                                                        >  2:2062218/1‑148 (MQ=1)

                                                        tCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGa                                                                                      >  1:1229307/1‑150 (MQ=35)

                                                        tCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGGTGGTAGAGCAGTTTACATTTAATCAAATGGTAGCAGGTTAGa                                                                                      >  2:1519424/1‑150 (MQ=11)

                                                             cGTAGTATCGGGTGATTAGCTCAGCTGGGAGGGCCCCAACCTTGACAGGAGGGGGTCGGCGGTTCGATACCGTCAACAACAACCAC‑CGCGGCGTTAGATTAGTTGGTAGTGCGGGTGACTTTTGGTGAGGTGGTCGCGGGTTCGATTcgt                                                                                 >  2:653092/1‑148 (MQ=2)

                                                               tagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                               >  1:4093252/1‑150 (MQ=34)

                                                               tagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                               <  2:2743812/150‑1 (MQ=34)

                                                               tagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGACATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                               >  1:1985282/1‑150 (MQ=21)

                                                                agtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACCAGGAGTGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                                                                              <  2:247696/150‑1 (MQ=18)

                                                                    tCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAc                                                                          >  1:1803009/1‑150 (MQ=33)

                                                                             tAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGtt                                                                 <  2:2042024/150‑1 (MQ=33)

                                                                              aGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGttt                                                                >  1:2304800/1‑150 (MQ=33)

                                                                              aGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGttt                                                                <  2:1128621/150‑1 (MQ=33)

                                                                                cTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTa                                                              >  1:446714/1‑150 (MQ=33)

                                                                                 tCAGCTTGGAGATCACCTCCCTTACAAGGAGGGGGTCGTAGGTTCTATCCCGTCATCACCAACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTaa                                                             <  1:4076961/150‑1 (MQ=11)

                                                                                 tCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑Cgg                                                                                                                                              <  2:2871221/69‑1 (MQ=35)

                                                                                 tCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTaa                                                             <  1:507470/150‑1 (MQ=33)

                                                                                    gCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAt                                                          <  2:2245681/150‑1 (MQ=33)

                                                                                    gCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAt                                                          <  2:898047/150‑1 (MQ=33)

                                                                                        ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGaa                                                      <  1:2678235/150‑1 (MQ=35)

                                                                                          tgagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATAACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCTAATCCTGCACGACCCACCAGTTTTAACATCGAAGa                                                    <  1:2008783/149‑1 (MQ=255)

                                                                                              cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCACAGGTGGTAGAGCAGTTGACTTTTAATCAAATGGTCGCACGTTAGAATCCTGCACCACCCACCAGTTTTAACAAAGAACACAGa                                                >  2:360474/1‑150 (MQ=11)

                                                                                                    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAAACAAATTTTAGCAGGTTCGAATACGGCACCACCCAACCCTTTTAAAAACAAAGACACATGTTaa                                          >  1:1932059/1‑150 (MQ=11)

                                                                                                          aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGTGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCgtgt                                    >  2:1999922/1‑150 (MQ=255)

                                                                                                           aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTCGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTCTTAACATCGAAGACAGATGTTAAGCGTGTa                                   <  1:1355373/150‑1 (MQ=255)

                                                                                                            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAg                                  <  1:3836917/150‑1 (MQ=255)

                                                                                                            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAg                                  <  2:3326173/150‑1 (MQ=255)

                                                                                                            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAg                                  <  1:3327866/150‑1 (MQ=255)

                                                                                                            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAg                                  <  1:3723904/150‑1 (MQ=255)

                                                                                                             gaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAgg                                 <  1:1663353/150‑1 (MQ=255)

                                                                                                                ggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGGGCCGGTGGTAGAGCAGTTGACATTTAATAAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAAAAAAAAAAAAACGTGTTAAGCGTGTAGGATa                              >  1:917084/1‑150 (MQ=11)

                                                                                                                 gggTCGGCGGTTCGATCCCGTCATCACCCACCAG‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATaa                             <  2:3943671/150‑1 (MQ=255)

                                                                                                                 gggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATaa                             <  1:3326404/150‑1 (MQ=255)

                                                                                                                    tCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGt                          >  2:2977634/1‑150 (MQ=255)

                                                                                                                       gcggTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGc                       >  2:1575103/1‑150 (MQ=255)

                                                                                                                        cggTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCg                      <  1:4091751/150‑1 (MQ=255)

                                                                                                                         ggTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCGGCACGACCCACCAGTTTTAACAAAGAAGACAGAGGGTAAGAGTGTAGGATAAAGGTGCGt                     >  1:2573477/1‑150 (MQ=14)

                                                                                                                           ttCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGTGGAGTTGACTTTTCATCTATTGGCCTCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCa                   <  1:650616/150‑1 (MQ=14)

                                                                                                                               aTCCCGTCATCACCCACCAC‑CGGGTCGTTAGGTCAGTTGGGAGAGCAGGTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACATTTAAAAACGAAGAAAGAAGTTAAGCGTGTAGGATAAAGTTGCGTCAGCaa               >  1:3351063/1‑150 (MQ=14)

                                                                                                                               aTCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCaa               >  2:2740632/1‑150 (MQ=255)

                                                                                                                                tCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAAc              <  1:2977634/150‑1 (MQ=255)

                                                                                                                                   cggcatcaCCCACCAC‑CGGGGCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGc           <  2:1361637/147‑1 (MQ=255)

                                                                                                                                    gTCATCACCAACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGcc          <  2:2153561/150‑1 (MQ=255)

                                                                                                                                        tcaCCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTa      <  2:4080836/150‑1 (MQ=255)

                                                                                                                                        tcaCCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTa      <  2:2304800/150‑1 (MQ=255)

                                                                                                                                            ccaccaC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgc  >  2:3242012/1‑150 (MQ=255)

CGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGC  >  NC_000913/780695‑780984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`