| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,581 | A→C | 22.6% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,581 | 0 | A | C | 22.6% | 132.8 / 15.3 | 99 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (24/51); new base C (4/20); total (28/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAA > NC_000913/164435‑164727 | tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCTAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:1436991/150‑1 (MQ=255) gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAg > 1:809722/1‑150 (MQ=255) tagaTTAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAAATTATTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTtca < 2:2044239/149‑1 (MQ=37) gagaTGAACGGGCTTTAGCCCACAAATGTCGGGCGGGACGACCCGGAAAATACTGCACCGCCGCGACGGACGAAAAATTTTTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGCCATCAGCGCGCACGTtca < 2:233831/150‑1 (MQ=25) gagaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca < 2:2776201/150‑1 (MQ=255) gagaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca < 2:2776278/150‑1 (MQ=255) agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggc > 1:411475/1‑146 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAACGCGCAGGtggaggc > 2:3280071/1‑144 (MQ=38) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc > 1:1413019/1‑150 (MQ=255) gAATGGGGGTTTTCCAAAATTTGTCTGGCCGGAGGCCCGGGAAACTACTGCACCGAGGCGCGGGACTAAAATTTTTTGTTATTGTTGTTTTTCTCCCTCTCCGTGTGGGATTGGGCGGGGGTTTGGGAATCAGCGCGCACGTtcaccctc < 2:1127772/150‑1 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccaca > 1:3876526/1‑150 (MQ=255) gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggacccgcaccc > 2:89373/1‑150 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCCaaa > 2:1474040/1‑149 (MQ=38) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCACACCCCAAcc > 1:4211319/1‑150 (MQ=38) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcgcccgcacccccaac > 1:1624298/1‑145 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCaaaac > 1:1624297/1‑148 (MQ=38) ttATCCCAAACAGGGCTGGCCGGACGACCCGGCAAAAACTGCCCCGACGCGCCGTACTAAAAAGTATTCTTACGGTGGTTTTTCTCCCTCCCCCTGTGGGCGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:291053/150‑1 (MQ=255) ccAAACTTGTGGGGCCGCACCCCCCGCAAAATACGCCCCCGCCGCGCCGGACCAAATAGTTTTCGTATGGTTGGTTTTCCCCCCCCCCCTGGGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:3066291/150‑1 (MQ=255) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCGCCCGCCCCCGCACCCCCGccc > 2:2982695/1‑150 (MQ=37) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCACCCGCCCCCAAACCCGCAcccaccc > 2:2510068/1‑146 (MQ=37) tgtggggCCGGACCCCCCGCCAAATACTGCACCGACGCCCCGCCCTAAACAGTTTTGGTATGGTGGGTTTTCGCCCTCCCCGGGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3943832/145‑1 (MQ=255) gtgtGGCCGGACGACCCGGAAAATACTGCACCTCCGCGACGGACTAAAAAGTTTTCTTACGGTTGGTTTTCTCCCACTCCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:4068293/147‑1 (MQ=37) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCAAACCCAAAACCACACCCAAAAggg > 2:244875/1‑150 (MQ=37) cgggCCGGACGCCCCGGCAAATACGGCACCGACGCGCCGGACGAAAAAGTTTTCGTAAGTTTGGTTTTCGCCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 2:547689/148‑1 (MQ=255) cgggCCGGACGACCCGGCAAATCCTGCACCGACGCGAGGGACGAAAAAGTTGTCGTATGGTTGGTTTTCTCCCTCTCCCGGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTTACCCTCTCCCTCAATGga < 2:3482144/148‑1 (MQ=37) gcgggACGACCGGGCAAATACTGCACGGCCGCGCGGTACGAAAAAGTTTTGTTATGGGTGTTTTTCTCCCTCTCCCTGTGGGCGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:1307330/147‑1 (MQ=25) gCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCGCCTTCGCCCTGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:2574445/150‑1 (MQ=255) cccGGCCGACCCGTCAAAAACTGCACCGCCGCGACGGACCAAAATGTATTCGTACGGTTGTTTTTCACCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 1:3767623/149‑1 (MQ=255) ccGGACGACCCGTAAAATCCTGCACCGAGGCGACGGACGAAAAATTTTACTTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:3545120/150‑1 (MQ=37) ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTTTCCGTACGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 2:3696314/150‑1 (MQ=38) ccGGACCACCGGCCAACTCCTGCACGGAGGCGAGGTACGAAATAGTTCTCTATTGTTTGGTTTTCGCCCTCCCCCTGGGGGTGTGGGCGGGGTTTTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 2:1627914/150‑1 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCAAAACCACAAGGGAggggggg > 2:3426600/1‑149 (MQ=37) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCAAACCCACTCCCGCACGGGAGAggggc > 2:1029593/1‑149 (MQ=37) cacgacCCGCAAAATCCTCCACCTACGCCCCGGACTAAAAATTTTTCTTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGGCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:2708890/149‑1 (MQ=21) ccgacCCGGCAAATTCTGCCCCGCCGCGACGCACGAAAAAGTATTCTTACGGTTGGTTTTCTCCCACTCCGTTTGTGAGCGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc < 2:2009846/149‑1 (MQ=255) cccGGAAATTAGTGCACCGCCGCGACGGACGAAAAAGTTTTCGTATGGTTGTTTTTCCCCCCCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 2:441466/150‑1 (MQ=38) ccGGCAAAGACTGCCCCGCCGCGACGGACTAACAAGTATTCGAACGGGGGGTTTTCTCCCGCACCCTGGGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:645723/150‑1 (MQ=255) ccGGAAAACACGGCACCGCCGCGCCGGACGAAAAAGTTTGCGTACGTTTGGTTTACGCCCTCTCCCTGGGGGAGCGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:1634120/150‑1 (MQ=255) ggCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:2411474/150‑1 (MQ=255) gCAAATACTGCACCGACGCGCCGGACGAACAAGTATTGGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:3876526/150‑1 (MQ=255) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCAACCGAACCCACTCCCACCCGGGCGAGGGGCCCGATCgcgcc > 1:3125608/1‑149 (MQ=25) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCCAAACCACAACCACAACGGGGTGGGGGCCGATAGAGcc > 2:291223/1‑149 (MQ=25) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCCCCCCAACCCACACCCCCAACGGGGGGGGGGCCCAACGaccc > 1:3697680/1‑147 (MQ=21) aaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCCCAAGGGAGAGGGGACCGATCGAGca‑c > 1:1799595/1‑150 (MQ=255) cTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAACCCACACCCCCAACGGGGGGGGGGCCGATAGAGCA‑Caaaa > 1:3560967/1‑149 (MQ=25) cACCGACGCGACGTACTAAAATGTATTCTTACGGTTGTTTTCCCCCCTCCCCCTGTGGTTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAAtttt < 1:2538491/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCAAACCCACACCCACAAGGGAGAGGGGACCCAACGAGCA‑CAAAattt > 2:3878760/1‑150 (MQ=37) cccGACGCGACGGACTAAAATGTTTTCTTATGGTTGTTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTg < 2:411475/149‑1 (MQ=38) cggacgggacgTACGAAAATGAATTCGTATGTTTGGTTTTCTCCATCGCCGTGTGGGTGTGGGCGGGGGTTTGGGAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTga < 2:3008704/148‑1 (MQ=255) ccgccgCGAGGGACGAAAAAGTTTTGGTAAGGTTGGTTTTCGCCCTCTCCGTGTGGGCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTga < 2:989074/150‑1 (MQ=255) ccGACGCGCCGTACCAAATATTATTCTTAGGGTTTGTTTTCACCCTGCCCGTGTGGTTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTga < 1:3398326/150‑1 (MQ=255) ccGACGCGCCGGACTAAAATGTATTGTTTATGTTGTTTTTCCCCCTCTCCGTGGGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTga < 2:3251727/150‑1 (MQ=255) ccGACGCGACGGACTAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCCCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTga < 1:3157682/150‑1 (MQ=38) cgacgcgacgGACTAAATTTTTTTCTTATGTTTTTTTTTCTCCCTCTCCCTGTGTTTGTGGGCGGGGGTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTgag < 1:656023/150‑1 (MQ=255) cgacgcgacgGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGACCCGCCCCCAAACCCACAACCACAACCGCGAGGGGGACGCACGGGCA‑Caaaaatttag > 1:604481/1‑143 (MQ=16) cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGTCCCACCCCCCAACCCACACCCCCAACGGCGTGGGGGCCGAGCGAGCC‑CAAAAATTgag > 2:1151080/1‑150 (MQ=21) cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAAGGGCGAGGGGGCCGCACGAGCACCAAAttttag > 1:4133127/1‑148 (MQ=25) cgcgacgGACTAAAAAGTATTCTTATGGTTGGTTTTATCCCTCTCCCTTTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTgagaga < 2:3073845/150‑1 (MQ=38) cgcgacgGACGAAAAAGTATTCTTAAGGTTGGTTTACTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTgagaga < 2:262084/150‑1 (MQ=255) gcgacgGCGGAAAATTTTTTCGTTTGGTTGTTTTTCTCCTTCTCCCTGGGGGAGTGGGCGGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGat < 2:4044595/150‑1 (MQ=255) gacggactAAAATGTATTGGTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGATTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGatat < 2:3421885/150‑1 (MQ=255) acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGCCCCACCCCCCAACCCACACCCCCCACGGCGCCGGGCCCCCTCGCGCC‑CCAAATTTGAGAGAGAtc > 1:2057285/1‑150 (MQ=12) gacgAAAAATTATTCGTATGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATAtcttc < 1:2011807/150‑1 (MQ=255) cgAAAACGTATACGTCAGGTTGCTTTACTCCCGCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATAtcttctt < 1:3438748/150‑1 (MQ=255) gAAAATGTTTTCGTACGGTGGGTTTTCGCCCTCTCCGTGGGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATAtcttcttc < 1:2687260/150‑1 (MQ=255) aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTc < 2:2484298/150‑1 (MQ=255) tCGTATGGTTGTTTTTCACCCTCTCCCTGTGGTTGTGGGCGGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:4023742/150‑1 (MQ=255) acggttggttTTCGCCCTCTCCCTGTGGTAGTGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:4088023/148‑1 (MQ=255) tgtttgtttTCCTCCCCCTCCGGGGGGTTGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:1285360/147‑1 (MQ=255) tggttgtttTTCTCCCACCCCCGGTGGTAGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:27338/149‑1 (MQ=255) cggttggttTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:2033135/149‑1 (MQ=255) gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCGAACCCACTCCCGCAAGGGGGAGGGGCCCGCTCGAGCA‑CAAAGTTTGGGAGATATCTTCTTATGTCGTGGTACagacga > 2:4207704/1‑150 (MQ=25) ggtggttTTCCCCCTCCCCCTGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:1212575/148‑1 (MQ=255) tgggttTCCCCCCCCGCCGTGGGGTAGTGGGCCGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 1:3216906/148‑1 (MQ=255) tggttTTCTCCCTCTCGCTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:2055821/150‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 2:2065972/150‑1 (MQ=255) tggttTTCCCCCCCGCCCTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:2372591/150‑1 (MQ=255) ttttCGCCCGCGCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 2:3652795/150‑1 (MQ=255) cctctcccaCAGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCTCCCCCACCCGAAACCACTCCCGCCATGGCGCGGGGCCCGTACGATCC‑CCAAGTTTGGGGGATTACGTCTTAGTGCTTGTATCCGCAGCACAGGAAATCgg > 2:1374367/1‑150 (MQ=255) cctccccctGGGGGTGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:2929938/150‑1 (MQ=255) ctctccctGTGGGAGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:604481/150‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:3507321/150‑1 (MQ=255) ctcgccctGTGGGAGCGGGCCGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCAACCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:406781/150‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:3206629/150‑1 (MQ=255) ggggAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:258570/149‑1 (MQ=255) ggAGAGGGCCGGGTTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:3878259/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCCCAAGGGAGAGGGGGCCCAACCACCA‑CAAAAATTGAGAGATATCATATTAAGTCTTGTAAAACAAGAACAAAAAAAAGGGCTTTTTCGCCGGAatat > 2:978566/1‑150 (MQ=255) gCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATTTCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt < 1:4133466/150‑1 (MQ=255) ggggggTTCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:3492/148‑1 (MQ=255) cgggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 1:161722/148‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:10832/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2129769/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1241899/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1880648/150‑1 (MQ=255) gggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:3757864/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:2225198/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:2225224/150‑1 (MQ=255) ggCCTCAGCGCGCAAGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATCTCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:1666054/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑AAAATTTTGAGAGATATCTTCTTCTGTCTTGAAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:1755540/150‑1 (MQ=255) | TGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCA‑CAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAA > NC_000913/164435‑164727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |