| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,613 | T→A | 8.0% | intergenic (+79/‑117) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,613 | 0 | T | A | 8.0% | 784.6 / 10.9 | 254 | intergenic (+79/‑117) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (32/191); new base A (22/2); total (61/193) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 282 total aligned reads displayed.
CCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGC > NC_000913/164466‑164762 | ccAAACTTGTCTGGCCGGACGACCCGTCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:6903706/150‑1 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCCCCCAAACCcacta > 1:6064634/1‑149 (MQ=37) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGAGGGACGAAAAAGTATTCGTATGTTTGTTTTT‑CTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:7012915/150‑1 (MQ=38) cAAACATGTCGGGCCGGACGCCCCGGCAAATCCGGCCCCGCGGGGACGGACGAAAATGTATTCGTACGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:4628663/150‑1 (MQ=37) cggcggggCCGCCCGCCCCGCCAAATACGTCACCGCCGCCCCGCACGAAAACGTATCCGGACGGTTGTTGGA‑GCCCCACTCGCTGGGGGTGAGGGCGGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:4190603/144‑1 (MQ=255) aTGTCTGGCCGGCCGACCGGGCAAATACTGCACCGACGGGAGGTACGAAATTGTTTTCGTATGGTTGGTTTT‑GTCCCTGTCGGTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:5207784/150‑1 (MQ=255) tttCTGGCCGGACGCCCCGCCAAATACTGCACCGACGCGACGTACGAAAAATTATTCGTATGGTTGGTTTT‑CCCCAGCCCCCTGGGGGATTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3499879/148‑1 (MQ=255) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCACCCGAAACCACACCCGCAAggg > 2:1379426/1‑150 (MQ=37) ggCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACCCCCAAACCCGCAACCACAAgggggg > 1:6313335/1‑147 (MQ=37) ccGGACCACCCGTAAAATCCTGCACCGACGCTACGTACTAAAATGTTTTCTTATGGTGGGTTTT‑CCCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 1:5969312/150‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGCCCCCACACCCCAAACCGCACCCCCACCGGAGAggg > 1:9331439/1‑150 (MQ=25) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:3930379/150‑1 (MQ=255) cctacCCGCAAAATCCTCCCCCGCCGCGCCGTACTAAATATTTTTCGTAGGGTTGTTTTT‑CCCCCTCTCCCTGTGGTAGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggtta < 1:8608242/147‑4 (MQ=255) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCCACCCCAAAACCACAACCACAACGGAGAGGGGCCCCATCg > 1:7774483/1‑150 (MQ=25) cGGCAAAGACGGCCCCGCCGCGACGGACGAAAACGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGTGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg < 2:647948/150‑1 (MQ=38) gCAAATCCGGCACCGAGGCGCCGGCCTAAAAAGTTTTCGTATGGTTGGTTTG‑CTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:8038232/150‑1 (MQ=255) caaaaaACTGCCCCGCCGCGACGGACCAAAATTTTTTCTTTCGGTTGTTTTT‑CCCCCTCGCCGTGTGGGTTTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:9213046/148‑1 (MQ=255) caaaaTACTGCCCCCCCGCGACGGACTAAAAATTTTTCTTACGGTTTTTTTT‑CTCCCCCACCCGGTGGGTGTGGGCGGGGGTTTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:3908711/148‑1 (MQ=255) atccTGCACCGACGCGACGTACTAAAAATTTTGCGTATGGTTGTTTTT‑CTCCCTCCCCCTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑caca < 1:9693884/147‑1 (MQ=255) ccgccgCGACGTACTAAAATGTTGTCGTATGGTTGGTTTT‑CCCCCTCTCCCTGGGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTga < 1:3021060/150‑1 (MQ=37) cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTTCACCCGCACCCTCACCCAAACCCACACCGGGGCGGGGACCCATCGCG‑CCCACAAttttag > 2:5577449/1‑148 (MQ=21) gaggcgccgGACGAAAAAGTATTCGTACGTTTGTTTTT‑CTCCCTCTCCCTTTGGGAGAGGGCCGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTgaga < 2:8721897/147‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCAAACCCTCTCCCCCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTtagag > 1:4291444/1‑150 (MQ=255) cgcgccgGACTAAAATGTTGTCTTAC‑GTTGGTTTTACCCCCACGCCCTGTGGGCGTGGGCGGGGTGGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTgagaga < 2:1852449/150‑1 (MQ=255) aaaaaGTATTCGTAAGGTTTGTTTT‑CTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATAtcttcttct < 2:5480745/150‑1 (MQ=255) aaaaTTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTg < 1:1230075/150‑1 (MQ=39) aaaaGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCCCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTg > 2:2285994/1‑150 (MQ=255) acgTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTc < 2:9285968/148‑1 (MQ=255) aaGTATTCGTATGGTTGGTTTT‑CTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTc < 1:5396041/150‑1 (MQ=39) tATGCGTATGGTTGGTTTT‑CTCCCTCCCCCGGGGGGTGAGGGCGGGGGGGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTg < 2:2678192/150‑1 (MQ=255) tttCGTAAGGTGGGTTGT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:7282081/149‑1 (MQ=255) aTTCTTAAGGTTGGATTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:2245014/150‑1 (MQ=255) tggTATGGTGGGTTTT‑CTCCCACCCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:7525898/148‑1 (MQ=255) gTACGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTaaaa < 2:9610921/150‑3 (MQ=255) tAAGGTTGGTTTC‑CTCCCTCGCCCTGTGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACag < 2:7650630/150‑1 (MQ=255) atggttggttTG‑CTCCCTCTCCCGGGGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:4999867/148‑1 (MQ=39) tggttgtttTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:9459006/149‑1 (MQ=255) tggttggttTT‑CTCCCTCTCCCTGTGGTTGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:4801186/149‑1 (MQ=255) tggttggttGT‑CTCCCACTCCGGGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:9591583/149‑1 (MQ=255) ggttTT‑CTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 2:6019568/150‑1 (MQ=255) tttt‑CTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:7090340/150‑1 (MQ=255) tttgcgccctcgcgcgGTGGGAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 2:1237331/144‑1 (MQ=255) gtgctccctctcgggGGGGGCGTGGGGGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaa < 2:2550021/147‑1 (MQ=255) ccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCACCCGCCCCCGAAACCACACCCACCCGGGGGGGGGGGCCCCGCGAG‑CACA‑AAATTTGGGCGGTATATTCTTCTGTGTTGTAACAGAAGAACAGcaa > 2:6113001/5‑150 (MQ=255) cctctccggGTGGGCGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑Cgg < 1:2099588/150‑1 (MQ=255) ccacaccctCTGGCAGAGGCCCGGGGTGAGGCCAACACCGCCCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAACGAG‑CACA‑AATATTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑Cgg < 2:3094224/145‑1 (MQ=255) ctctccgtGTGGGTGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑Cggg < 1:1144726/150‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCCAAACCACTCCCACCAGGGAGAGGGGGCCCCTAGAG‑CCCA‑AAATTTGGGAGATATATTAATATGTCTTGTTACAGAAGAACAGAAAAT‑Cggg > 2:7999694/1‑150 (MQ=21) ggtgGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTgc < 1:1554108/149‑1 (MQ=255) gagtGGGCGGGGGTGCGGGCCTCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTg < 1:256502/150‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTg < 1:5754414/150‑1 (MQ=255) agagGGCCGGGGTGAGGGCATCAGCGCGCACGGGGACCCCCAACCCAACCCACAACCACAAGGGGGGGGGGGCCCCTCCCGCCCCA‑AATTTTG‑GAGATATAATAATAGGTGTTGTTAAAGAAGAACAGAAAATACGGCCTTTTggcccgg > 2:4846065/1‑144 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCACCCGAAAACCCACCCACCACGGAGGGGGGGCCCAACGAG‑CACA‑AAAATTGAGGGATAAAATAATAATTATTGTAAAAAAAAAAAAGAAAAA‑CCGGCTTTTTCGCCGGaaaaa > 2:7942286/1‑147 (MQ=255) cGGGGTGCGGCCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGc < 1:7381562/150‑1 (MQ=255) gggtTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 2:5790433/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 2:7884337/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 1:9195021/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 1:4023986/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 2:3223863/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCg < 2:5162255/150‑1 (MQ=255) gttGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGga < 2:8357461/148‑1 (MQ=255) ggAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGgaga < 2:6583722/149‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 1:1379358/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAATAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:5749873/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:9454310/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:7607479/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:296743/150‑1 (MQ=255) gcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCggg < 1:5577449/150‑1 (MQ=255) ccGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa < 1:3689025/149‑1 (MQ=255) ttCACCCTCACCCTACCGCTCTCCCTCAAGGGCGAGGGGACCGATCGAG‑CACA‑AATTTTGAGTGTTATCTTCTTCTGTCTTGTTACAGATGAACAGAAAAT‑CGGGCTTTTGCGCGTGAATATTGCGGAGAAATAGCATGGCCGGGTATGa < 1:8933445/150‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAc < 2:8723515/150‑1 (MQ=255) caccctcaccctAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATATTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAcc < 1:2421456/150‑1 (MQ=255) ccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgc < 1:8525854/150‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg > 2:686633/1‑150 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 2:4940559/1‑150 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg > 1:2681508/1‑150 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg < 2:1498456/150‑1 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg < 2:5996249/150‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCa < 1:5709061/150‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCa < 1:198442/150‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCa < 1:6832780/150‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGCCaa > 1:8302540/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 1:1730797/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 1:7944282/1‑150 (MQ=255) taccccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 1:3317464/147‑1 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 2:3374224/150‑1 (MQ=255) gaaCCCTCTCCCACAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGCGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 1:4624168/149‑1 (MQ=255) tccccTCTCCCTTGAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCCATTg > 2:7128646/3‑150 (MQ=255) ccccCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 1:2286138/150‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 1:3554817/150‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 1:2589656/150‑1 (MQ=255) cccTCTCCCGTGAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAATCATGGCCGGGAATGACCGCGAGCCAATTGGa > 1:1335409/1‑150 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc > 2:3654299/1‑150 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATGTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAG‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc > 2:9589468/1‑150 (MQ=255) ctctCCCTCCCGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGTTTTCTTCTTCTGTCTTGTACCAGAAGAACAGTAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCACTTGGACg < 2:1307269/150‑1 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:5918616/1‑150 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:7841115/1‑150 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc > 2:7626497/1‑150 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc > 1:2190192/1‑150 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc > 2:290264/1‑150 (MQ=255) | CCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAT‑CGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGC > NC_000913/164466‑164762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |