| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,648,458 | T→G | 7.5% | N1277T (AAC→ACC) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,648,458 | 0 | T | G | 7.5% | 355.8 / 15.8 | 140 | N1277T (AAC→ACC) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (62/66); new base G (4/7); total (67/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
CACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGG > NC_000913/2648312‑2648604 | cACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTaa > 2:1303663/1‑150 (MQ=255) cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATg < 1:1748862/150‑1 (MQ=255) cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATg > 2:1613227/1‑150 (MQ=255) ccATCGGAGCCTTTTGCTTACGGGCCAACACCAGCGCAGCGTATGACTGTTCGTCGAATTTAGTGGCTTTGAGATTATCGGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATg‑g < 2:1004056/150‑1 (MQ=255) aGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCg < 2:820049/150‑1 (MQ=255) gCGGAGCCTTTTGCTGACGGGCCAACACCCGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGt < 1:1495793/150‑1 (MQ=255) cGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTc > 1:229139/1‑150 (MQ=255) ggAGCCTTTTGCTGACGGGCCAACACCAGCGCCGCGTAAGACTGTACAGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTTTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCt < 2:294593/150‑1 (MQ=255) gAGCCTTTTGCTGACGGGCCAACCCAAGCGCAGCGTAAGCCTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTCCCCCGGTTAATG‑GCGACTg > 2:150113/1‑150 (MQ=255) gAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTg < 1:1260073/150‑1 (MQ=255) aGCCTTTTGCTGCCGGGCCCACACCAGCGCAGCGTAAGACTGTACGGCGACTTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc < 2:31985/150‑2 (MQ=255) aGCCTTTTGCTGACGGTCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTGGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc < 2:1126282/150‑2 (MQ=255) aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTCCTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGTTAATGAGCGTCTgc < 2:987202/150‑2 (MQ=255) aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGt < 1:1559701/150‑1 (MQ=255) aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgc < 2:1001433/150‑2 (MQ=255) gCCTTTTGCTGACGGGCCAAACCCATCGAAGCGTAATACTGTCCGTCTAATTTACTGGCTATGAGATTATCCGCGTACGGTATCGACATCATTCCCGGATCTTGTAAATAGCGCAGAAGACGC‑CATTAACCGGGGTAATGAGCGTCTgct < 2:1304224/150‑3 (MQ=255) ccTTTTGCTGCCGGGCCAACACCAGCGCAGCGTAAGACTGGACGGCGAATATACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCg < 2:1215312/150‑1 (MQ=255) cTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGC‑CATTAACCGGGGTAATGAGCGTCTgctgc < 1:883705/150‑5 (MQ=38) ttttGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGc > 1:1461997/1‑150 (MQ=255) tttGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTGTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCa < 2:1013549/150‑1 (MQ=255) gACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGt > 2:1572610/1‑150 (MQ=255) ccGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTa < 1:1031671/149‑1 (MQ=255) aCGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTa < 2:1809818/150‑1 (MQ=255) ggacAACACCCGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGAGCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAAcc < 1:1261924/147‑1 (MQ=255) ccAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGT‑ACCCTg > 2:1775034/1‑150 (MQ=255) cAACCCCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTg < 2:96057/150‑1 (MQ=255) cacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTc > 2:141009/1‑150 (MQ=255) cacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCCGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTc > 1:784764/1‑150 (MQ=255) cacCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTATGAGATTATCCGCGTACGGGATCGCCATCATGACCGGCACTTGTAAATAGCTCAGCAGACGCTCATTCCCCCGGTAAATG‑GCGTCTGTCGGCACGCTCTACCCCTCTTc > 2:1157559/1‑150 (MQ=255) ccAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATTGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGc > 2:158934/1‑150 (MQ=255) ccAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGAAGACGCTCATTACCCCGGTTAAAG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGc > 2:1777391/1‑150 (MQ=255) ccAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGACCGGATCTTGTAAATAGCGCAGCAGACGCTAATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGAAACCCTGTTCGc > 2:1770035/1‑150 (MQ=255) cagcgcagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGcc < 1:1321514/150‑1 (MQ=255) cagcgTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcgc < 2:1035879/150‑1 (MQ=255) aGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAg < 1:1821668/150‑1 (MQ=255) aCTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGa > 1:561236/1‑150 (MQ=255) gTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAAt < 1:420780/150‑1 (MQ=255) gTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAAt < 1:29280/150‑1 (MQ=255) aCGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATcc < 2:841290/150‑1 (MQ=255) cGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCa > 1:1173906/1‑150 (MQ=255) ggCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGTCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCAt < 1:71686/150‑1 (MQ=255) ggCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCAt > 2:1087519/1‑150 (MQ=255) ggCGAAGTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCTGATAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGCCCAGGAAATCCAt > 2:899020/1‑150 (MQ=255) gCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATc > 2:10936/1‑150 (MQ=255) gCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATc > 1:1088028/1‑150 (MQ=255) cGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCCTGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGACGGCACGCTGTTACCCTGTTCGCCTGCGCGGACCAGGATATCCATCa > 2:91363/1‑150 (MQ=255) cGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATcc > 2:462337/1‑149 (MQ=255) tACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAg < 2:68345/150‑1 (MQ=255) gCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTc < 2:486647/150‑1 (MQ=255) cTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCa > 1:47423/1‑150 (MQ=255) tGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGcc > 1:690485/1‑150 (MQ=255) tGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGcc > 2:1822710/1‑150 (MQ=255) ttATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTAc > 2:1693183/1‑150 (MQ=255) aTCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTActc < 1:999280/150‑1 (MQ=255) ccGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCtt > 2:1834853/1‑150 (MQ=255) cgTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGt > 1:741812/1‑150 (MQ=255) ggaCGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTc < 1:607689/148‑1 (MQ=255) gTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTc < 2:510026/150‑1 (MQ=255) cGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCAcc > 2:1385744/1‑150 (MQ=255) gggATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCg < 1:1303663/150‑1 (MQ=255) ggATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGt > 1:1589234/1‑150 (MQ=255) ggATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATACATAACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGt > 1:1155263/1‑150 (MQ=255) aCATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTAt < 2:1234836/150‑1 (MQ=255) aatcatGCGCGGTTGTTGTAATTAGCGCTGCAGACGCTCATTACCGCGTTTTTTG‑GCGTCTGTCGGCACGCTGTAACTCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATc < 1:150113/149‑1 (MQ=255) atcatGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATcc < 2:1049625/150‑1 (MQ=255) tcatGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATccc < 1:1570303/150‑1 (MQ=255) catGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCca > 1:1459312/1‑150 (MQ=255) tGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCcaca < 1:1529193/150‑1 (MQ=255) tGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCcaca < 1:820766/150‑1 (MQ=255) gCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGACATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCcaccg > 1:1516300/1‑148 (MQ=255) cccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgc < 1:889924/150‑1 (MQ=255) ccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgcg < 1:1733185/150‑1 (MQ=255) ccGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAgcc > 2:158292/1‑149 (MQ=255) cTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgc > 1:195218/1‑150 (MQ=255) gTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAgccgc > 2:515581/1‑150 (MQ=255) aaTAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGt > 2:1700880/1‑150 (MQ=255) aTAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGtt < 2:357983/150‑1 (MQ=255) aGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTAt > 1:327202/1‑150 (MQ=255) gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAACTCCATCCCGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc > 1:264682/1‑150 (MQ=255) gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc > 1:921413/1‑150 (MQ=255) gcgcAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATc > 1:1758027/1‑150 (MQ=255) gcagcaGACGCTCATTACCCCGGTTTATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAACTCCATCACGTAAGCCGTCCGCCAGTACTCTTCGTCCGCGTTTTTATCCCCCAGCGCAAAGCCGCCGTTATctc > 2:282508/1‑148 (MQ=255) cagcagACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACg > 2:690300/1‑150 (MQ=255) gacagACGC‑CATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGt > 2:1635422/3‑150 (MQ=255) gcagACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGGTATCACGtt > 2:188206/1‑150 (MQ=255) agACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc < 1:703246/150‑1 (MQ=255) agACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTATTCTACACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGc > 2:245014/1‑150 (MQ=255) gACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCCCAGCGCAAAGCCGCCGTTATCACGTTGCa > 2:239987/1‑150 (MQ=255) gacgccATTAACCGGGGTAATGAGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTAGCACGTTGCa > 1:477173/6‑150 (MQ=255) aCGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCAt < 2:897723/150‑1 (MQ=255) aCGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCAt < 1:1265715/150‑1 (MQ=255) cGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCATGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTCAGCCTTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCAtt < 2:1065161/150‑1 (MQ=255) tCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgc < 2:637888/150‑1 (MQ=255) tCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgc > 1:1507312/1‑150 (MQ=255) tCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgc < 1:1054087/150‑1 (MQ=255) cATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCCGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgca > 1:796571/1‑150 (MQ=255) ttACCCCGTTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTgcagc < 2:229139/150‑1 (MQ=255) ccGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACg < 2:47423/150‑1 (MQ=255) ccGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACg < 2:1854133/150‑1 (MQ=255) gggTAAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACgg < 1:248219/149‑1 (MQ=255) | CACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATG‑GCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCGCGGACCAGGAAATCCATCACGTAAGCCGTCAGCCAGTACTCTTCGTCACCGTTTTTATCCCACAGCGCAAAGCCGCCGTTATCACGTTGCATTTGCAGCAAACGG > NC_000913/2648312‑2648604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |