| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 657,533 | A→T | 6.8% | intergenic (+32/+22) | cspE → / ← flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 657,533 | 0 | A | T | 6.8% | 298.1 / 6.9 | 108 | intergenic (+32/+22) | cspE/flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (47/52); new base T (3/5); total (50/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACT > NC_000913/657390‑657681 | ctctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg < 1:314318/150‑1 (MQ=255) tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑t > 1:1077767/1‑150 (MQ=255) gCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tt > 2:642354/1‑150 (MQ=255) gCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tt > 2:1778654/1‑150 (MQ=255) aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTTCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 2:592309/150‑1 (MQ=255) aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 1:1159790/150‑1 (MQ=255) aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 2:220475/150‑1 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑ttttt > 1:1221463/1‑150 (MQ=255) ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGAAAGATACGTCAGCAAGAATTCAAAACCCCCTTAATCAGCGGG‑ttttttt > 1:1186645/1‑150 (MQ=255) cAGACTAATGGTTTTAAAACTCTTGCTGAAGGGCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tttttttt < 2:1401429/150‑1 (MQ=255) cAGACTAATGGTCTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGAAAGAATTCAAAACCCGCTTAATCAGCGGG‑tttttttt > 2:1518148/1‑150 (MQ=255) ataCTAATTGTTTTATAACTCTTGCTGAATGTCAGCGCGTAGAGTTCGTAATCACTAACGGTGCCAAAGGGCCTTATGCTGCAAACGTAATCGCTCTTTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTg < 1:675072/148‑1 (MQ=255) aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTg < 1:839823/150‑1 (MQ=255) aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTg < 2:189233/150‑1 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTgg < 1:483309/150‑1 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGGCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCTAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTgg < 1:1312778/150‑1 (MQ=255) aaTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAATCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCtt < 1:131893/150‑1 (MQ=255) aTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCttt < 1:189799/150‑1 (MQ=255) tGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTa > 1:1565408/1‑150 (MQ=255) gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAgt > 2:451577/1‑150 (MQ=255) tttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCGTAATCAGCGGG‑TTTTTTTTGGTCTTTAgtgt > 2:1619914/1‑150 (MQ=255) ttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGGTCGTAATCACAAACGGTGCCTAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTAAATCAGCGGG‑TTTTTTTTGGTCTTTAGtgtg < 1:1757450/150‑1 (MQ=255) tAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTATtgtgg > 2:356790/1‑149 (MQ=255) tAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGc > 1:465803/1‑150 (MQ=255) aaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCgg < 1:811024/150‑1 (MQ=255) aaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCgg < 1:760602/150‑1 (MQ=255) tctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGa > 2:1363141/1‑150 (MQ=255) gCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg < 2:188532/150‑1 (MQ=255) ggTCAGCGCGTAGAGTTCGAAATCACTAACTGTGCCAAAGGCCCTTATTCTGCAAACGTAATCGCTCTGTAATATACGTCAGCAAGAATTCAAAACCCGCTTAATAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc < 2:263430/150‑1 (MQ=255) gTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGAATAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa > 2:270075/1‑150 (MQ=255) tCAGCGCGTAGAGTTCGAAATCACTAACTGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTAAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg < 2:909243/150‑1 (MQ=255) tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg > 1:475158/1‑150 (MQ=255) tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg < 2:1369155/150‑1 (MQ=255) aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGCGGCCGAAAACAGcc > 2:1265752/1‑150 (MQ=255) tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATg < 2:959025/150‑1 (MQ=255) agTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCa > 2:1107136/1‑150 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg < 2:1284859/150‑1 (MQ=255) gTTCGAAAGCACTAACGGTGCCACAGGCCCTTCTGCATCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTATTCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg < 2:82686/150‑1 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTATTCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc > 2:808607/1‑150 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc < 2:1767105/150‑1 (MQ=255) gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg > 2:1632426/1‑150 (MQ=255) aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGt < 2:172126/150‑1 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCa > 1:994192/1‑150 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCa > 2:931712/1‑150 (MQ=255) cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGATCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGTG‑‑TTTTTTTGGTCTTTAGTGTGCGGTTGAGTCCGAAAACAGCCAGAATGCCAGTGCGGTCATg > 2:1329283/1‑150 (MQ=255) aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg > 2:900530/1‑150 (MQ=255) cTAACGGTGCTAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg > 1:939715/1‑150 (MQ=255) tAACTGTGCCAAAGGCCCTTATGCTGCAATCGTAATCTCTCTGTAATATACGTCAGCAAGTATTCAAAACCCGCTTAATAAGCGGG‑TTTTTTTTTGTCTTTAGTGTGCGGTTGAGGCCGAATACAGCCAGAATGCCAGTGCGGTCATGGc < 2:672420/150‑1 (MQ=255) aCGGTGCCAAAGTCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGT‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaa < 1:987379/150‑1 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 2:1514875/150‑1 (MQ=255) ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc < 2:1723311/150‑1 (MQ=255) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc < 2:566461/150‑1 (MQ=255) cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCACCGGG‑TTTTTTTTGCTCTTTTGTGTGCGGTTGAGGACGAAAACAGCCAGAATGCCTGTGCGGTCAAGGCAAAAGACCAcagcag > 2:1233498/1‑150 (MQ=255) tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg > 2:97676/1‑150 (MQ=255) ctgctgCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGa < 2:576386/150‑1 (MQ=255) tgctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc < 2:638175/150‑1 (MQ=255) tgcgccAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 2:1186645/145‑1 (MQ=255) gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 2:465803/150‑1 (MQ=255) gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 2:804297/150‑1 (MQ=255) gcggcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 2:1226504/147‑1 (MQ=255) ctgcAAACGTAATCGCTCTGTAAGATACGGCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGa < 1:1243516/150‑1 (MQ=255) tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa > 1:45834/1‑150 (MQ=255) aaaCTTAATGGCTCTGTAAGATACCTCAGCAAGAATTCAACACCCGCTAAATCATCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAc < 2:330433/150‑1 (MQ=255) aaaCGTAATCGCTCTGTAAGATACGGCAGCAAGAATTCAAAACCCGCTTACTCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGGTGACGAAAAc < 2:916359/150‑1 (MQ=255) aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg > 2:413724/1‑150 (MQ=255) cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt > 1:1226536/1‑150 (MQ=255) gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc < 1:1701615/150‑1 (MQ=255) tactCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCa < 1:1499951/147‑1 (MQ=255) tAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCa > 2:1540628/1‑150 (MQ=255) aaTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCTGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCACAGAAGGTTTACGAAAACGTTCAg > 1:361149/1‑150 (MQ=255) aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGt > 1:1692334/1‑150 (MQ=255) tCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGCCCCCAGAAGGTTGACGAAAACGTTCAGTa > 2:1663522/1‑150 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATg < 1:1047946/150‑1 (MQ=255) gTAATATACGTCATCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCa < 2:1622193/150‑1 (MQ=255) gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGATAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGGTGACGAAAACGTTAAGTAATGgccc > 1:352851/1‑149 (MQ=255) gTAAGATACGTCAGCAAGAATCCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCa < 2:1100315/150‑1 (MQ=255) tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAg > 1:1823957/1‑150 (MQ=255) tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAg < 2:1701911/150‑1 (MQ=255) gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCa > 2:454074/1‑150 (MQ=255) cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGc > 1:1444229/1‑150 (MQ=255) cAGCAAGAATTCAAAAACCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAATGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGc < 1:1644008/150‑1 (MQ=255) gCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACCGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGccc > 1:1035192/1‑150 (MQ=255) cAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTAGAGGCCGAAAACAGCCAGAATGCCAGTCCGGACATGGAAAAAGACCCCAGAAGGTTGCCGAAAACGTTCAGTAATGCCCAGCCAAAGAGGcccg > 2:132957/1‑149 (MQ=255) gAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCATAGCGGCCCTCtt > 1:1357113/1‑150 (MQ=255) aaaaaaaaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGt > 1:778112/5‑150 (MQ=255) aTTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGCGCGTTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCATCTTGt > 2:419747/1‑150 (MQ=255) tCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTaa > 2:382742/1‑150 (MQ=255) tCAAAACCAGCTTCATCAGCGGG‑TTTTTTTTTGTCTTTAGTGTGCGGTTGAGGCCTAAAACAGACAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa < 2:1257609/150‑1 (MQ=255) cAAAAGCCGCTTAATCAGCGGG‑TTTTTTTTTGTCTTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCATAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc < 1:1167387/150‑1 (MQ=255) cAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCGTCTTGTatc > 2:894748/1‑148 (MQ=255) aacaCCCGCTTAATCAGCGGG‑TTTTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAACGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa < 1:920961/147‑1 (MQ=255) aaaaCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa < 1:1518148/150‑1 (MQ=255) aaaaCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGATGAGGCCGAAAACAGACAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa < 1:270075/150‑1 (MQ=255) aaaCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaa > 1:438395/1‑150 (MQ=255) aaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAAAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAAGAAAACGTTCAGTAATTCCCAGCCAAAGCGGCCCTCTTGTTACaaa > 1:1577212/1‑150 (MQ=255) aCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaa < 2:1796407/150‑1 (MQ=255) aCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaa < 2:1221463/150‑1 (MQ=255) ttAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacca < 1:1476707/150‑1 (MQ=255) tAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac < 1:1491843/150‑1 (MQ=255) aaTCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTGCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACt > 2:257673/1‑150 (MQ=255) | CTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACT > NC_000913/657390‑657681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |