| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,296,061 | 0 | C | T | 30.0% | 45.8 / 27.4 | 42 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (17/11); new base T (7/5); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC > NC_000913_3_bme_pgi/4295913‑4296207 | gttgggtAAGGCGTTTAGGCCGCATCCGACAGCAACGCCTGATGCGACGCTTAACGCGTATTATCAGGCCTATGAAACCTCGAGGATAAGTCTGATGCAGCGTCACTGGTTAG‑GTCGGATAAGGCGTTAACGCCCCATCCGACATCAAc < 2:516629/143‑1 (MQ=2) tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg > 2:438705/1‑149 (MQ=32) tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCAGATGCGACGCTTAACGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTACCGTGACTTCGAAG‑GTCGGATAATGCCTTCACGCCCCAACCCACACCAAca > 2:242831/1‑148 (MQ=11) ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc < 1:501055/149‑1 (MQ=32) tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCAGGTTTAGCATGATTTTGTAG‑GACGGATACGGCGTTTACACCGCATCCGACATCAACGCCTGa > 2:19383/1‑149 (MQ=11) aaGGCGTTTATGCCGCACCCGAGATCAACGCCGGATCCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt < 1:167796/149‑1 (MQ=11) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTGTTAACGGTCGGACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGGAG‑GTCGGATAAGGCGTTTACGCCCCATCCGCCCTCAACGCCGGATGc > 1:104019/1‑149 (MQ=11) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTCTACGCCGCATCCGACATCAATGCCTGATGc < 1:129425/149‑1 (MQ=17) gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg > 2:283096/1‑149 (MQ=17) cttctATGCCGCAGCCCGCAGCAAGGCCTGATGCGACGCTCAACGCGTCTTATCAGGCCTACGGCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCATGATGCGa < 2:334164/145‑1 (MQ=11) gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc > 1:338681/1‑149 (MQ=17) tgtATTCCGCATCCGCCATCCACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg < 1:62056/147‑1 (MQ=11) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc > 1:494021/1‑149 (MQ=21) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGAAAGCGGAATAGCCTGATTTAGCGTGATTTTGTAG‑GTGGGATAGGGCGTTTACGCCGCATCCGACATCACAGCCTGATGCGAAGCttaca > 1:269763/1‑146 (MQ=11) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg > 2:98025/1‑149 (MQ=25) tgggagaTCAACGCCTGATGCGACGCTTAATGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc < 2:131095/143‑1 (MQ=255) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa > 1:457635/1‑149 (MQ=25) aaaTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:283214/147‑1 (MQ=39) tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 1:400156/1‑149 (MQ=21) tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:283096/149‑1 (MQ=21) aaCGCCTGACGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:4027/149‑1 (MQ=255) caccTGATGCCACGCTGAACACGACTTAGCAGGACTACGCCAGCCAGCACAATAGACGGATCTAGCGTGATTTTCTAG‑GACGGATAAGGCGTTTACGCCGCATCCGACATCGCTTCCTGATGCGACGGTTGCTGCGTCTTAACAGGCCt > 2:76751/3‑149 (MQ=19) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg > 2:18354/1‑149 (MQ=21) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:473523/124‑1 (MQ=25) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:473523/1‑124 (MQ=40) ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAAGGCCTGATGCGACGCTTGCCGCGTCTTATAAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCg > 2:197202/1‑149 (MQ=255) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAAGCGTTTACGCCGCATCCGACATCCACGCCTGATGCGACGCTTGACGCGTCTTATCGGGACTACGCTAGACAACGCCTTATCCTGATTTAGCGtcac > 2:188260/1‑146 (MQ=11) gCCAGACAGCGCAATAGCCTGATTTAGCGAGATTTTGTGG‑GTCGAATAAGTCGTATATGCCACATCCGACGTCAACGCCTGATGCGACGCTTGCCGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCtttt > 1:454205/1‑149 (MQ=11) aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTGTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg > 2:492504/1‑149 (MQ=37) gcgcAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg < 1:492504/149‑1 (MQ=39) aaTAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCATAAGCCTGATTTAGCGTGATTTTGTAGGTCggtca > 2:404144/1‑146 (MQ=255) ttAGGGTGATTTTGTAGTGACGG‑TAAGGCATTTCCCCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg < 2:472914/149‑1 (MQ=11) tGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAt > 1:60613/1‑149 (MQ=38) gATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATc > 2:302966/1‑149 (MQ=38) gATTTTGGAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATc < 1:376875/149‑1 (MQ=255) g‑gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCCTCaa > 1:75925/1‑149 (MQ=255) gacagggCGTGTCCGCCGCGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt < 2:186579/144‑1 (MQ=255) gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCt > 2:534136/1‑149 (MQ=255) gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc > 1:364444/1‑149 (MQ=38) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTATGCCGCGTCCGACATCAATGCCTGATTCGAcc > 2:120734/1‑148 (MQ=255) ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc < 1:302966/149‑1 (MQ=35) gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGTATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa > 1:527619/1‑149 (MQ=25) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg < 1:18354/149‑1 (MQ=33) aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTCTCAGGc > 1:193703/1‑149 (MQ=17) | GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAG‑GTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC > NC_000913_3_bme_pgi/4295913‑4296207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |