| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 3,966,742 | G→T | 22.8% | R109L (CGC→CTC) | rho → | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 3,966,742 | 0 | G | T | 22.8% | 90.8 / 26.8 | 67 | R109L (CGC→CTC) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base G (32/18); new base T (8/7); total (42/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01 | |||||||||||
GATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA > NC_000913_3_bme_pgi/3966594‑3966888 | gatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTCATACCATCTTTGGTAAGATTCGCCCGCCGAAAAAAGGTA‑AACg > 1:157242/1‑149 (MQ=255) gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAACGTG‑ACCGCtct > 1:441009/1‑147 (MQ=255) atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTAtt > 2:262529/1‑149 (MQ=255) atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTGCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTAtt < 1:863224/149‑1 (MQ=255) tttGGTTTCCTCCGTTCAGCAGACAGATCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGACAAATCCGCCGTTTCAACCTCCGCACTGGTGATCCCATTCCTGGAAAAATACGCCAGCCGAAAGAAGGGG‑AACCCAAttc > 2:897394/1‑148 (MQ=255) ttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCGTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCACGACAAGAAGGTG‑AAGGCTAtttt > 1:792101/1‑149 (MQ=255) tGGTTGCCTCCTTTCCGCAACCCGCTCCTACCACGCCGTGCCTTATTACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTg < 1:244372/149‑1 (MQ=255) tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTGTCCCCTCGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTgggc > 1:173164/1‑149 (MQ=255) ttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCACCCGCCGAAAGAAGACG‑AAGACGATTTTGCgct > 1:156210/1‑149 (MQ=255) ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCgctgct > 1:561194/1‑149 (MQ=255) ctccGTTCCGCAGACAGCTCATACCTCGTCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCGCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCgctgct < 1:386401/149‑1 (MQ=255) tccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATAACATCTACGTTTCACCTAGCCAAATCCGCCGTTTCAACCTCCGCACAGCAGGACCCGTGTTGGGTAAGATTCGCCCGCCGCAGGAAGGTG‑AACGCTGTTTTACgctgcta > 1:429109/1‑148 (MQ=255) agcggACAGTGCCGACCTCGACGGTCCGGATGACATCTACGTTTCACCTAGCCAAAGCCGCTGTTTCAACCTACGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGGCGAAAGCAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTa < 2:517851/145‑1 (MQ=255) gCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTaa > 1:411998/1‑149 (MQ=255) gACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGa < 1:694635/149‑1 (MQ=255) cctccctcGCCGGTCCTTATTACATTTTCGTTTCCCTTATTCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCGGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAAc < 1:140090/149‑1 (MQ=255) cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTTTCAGTACTTACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑TACGTTATTTTGGGCTGCTGAAAGTTAACGAAGTTAACt < 1:429977/149‑1 (MQ=255) cTCGCCGGTCCTGATGACATATACGGTTCCCCGAACGAAATCCGCAGTTGCAACATCCGCACTGGTGAGACCAGCTCTGGTAAGATTCGCCCGCCGAAAGCAGTTG‑AACGCAATTTTGATCTACTGCAAGTTACCAAAGTTAACTTctc > 2:843085/1‑147 (MQ=255) gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa > 1:476683/1‑149 (MQ=255) gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt > 1:50798/1‑149 (MQ=255) tgatgaCATCTACGCTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGCTGATACCATCTCTGGTAAGCTTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGCTAACTCCGACAAACCTGaaa > 2:628451/1‑149 (MQ=255) gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAGGGTG‑AACGCTATTTTGAGCGGCTGAAAGTTAACGAAGTTAACTTCGACAGACCTGacaa > 2:495237/1‑149 (MQ=255) gatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaaa < 1:463157/149‑1 (MQ=255) tgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATGCCCTCTCTGGTAAGATTCGCCCGCCGAAAGACGGTG‑AAGGCTATTTGGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCGGaaaaag > 2:726729/1‑147 (MQ=255) aTCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg > 2:471966/1‑149 (MQ=255) tCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTTTAGTAGGATTTGCCTTCTGAAA‑ATGGAGTAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAATACTGAAAACGCCCga > 2:676913/1‑148 (MQ=255) tACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGATGCTGAAAGTTAACGAAGTTAACTTCGACAACCCTGAAAAAGccaccac > 2:48309/1‑144 (MQ=255) tttCCCCTAGCCAAATCCGGCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTATGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaccaac < 1:854402/149‑2 (MQ=255) tCCCCTAGCCAAATCCGGCGTTTCAACCTCCTCCCTGGTGATACCATCTCTTGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaagg < 2:855573/149‑3 (MQ=255) ccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATCCGCCCGCCGAAAGAAGGTA‑AACGCTATTTTGCGCTGCTGAAAGGTAACGAAGTTAACTTCCACAAACCTGCAAACGCCCGCAACACACTCct > 2:727731/1‑149 (MQ=255) cTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc > 1:580467/1‑149 (MQ=255) cTAGCCAAATCCGCCGTTTAAACCTCCGCACTGGTGATACCATATCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCGCTTTTGCGCTGCTGAAAGTTAACGACGTTAACGTCGACAAACCGGAAGACGCCCGCAACAAAATCctc > 2:861527/1‑149 (MQ=255) tAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctct > 2:609808/1‑149 (MQ=255) ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg > 1:159508/1‑149 (MQ=255) ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg > 1:849953/1‑149 (MQ=255) cAAACCCGCCGTTTCAACCTCCGAACAGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga < 2:827044/149‑1 (MQ=255) tCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTGACTTCGACAAACCTGAAAACGTCCGCAACAAAATCCTCTTTGAGAAc > 1:116790/1‑149 (MQ=255) tCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc < 1:539150/149‑1 (MQ=255) tCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc > 1:493120/1‑149 (MQ=255) ccgccgTTTGATAGTCCGCGTGGGTGCTACCATCTCTGGTAAGATTGGCCGGGCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCGGAAAGTTAACGAAGGTAACTTCGACAAACCTGAAAACGGCCGCAACAAAATCCTCTGTGAGAACt < 2:868138/149‑1 (MQ=255) gccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCAGCAACAAAATACTCTTTAAGAACTTa > 1:743630/1‑149 (MQ=255) tttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACAAATTTAACTACGACAAACCTGACAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcac > 2:738806/1‑147 (MQ=255) aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACGACGACAAACCGAAAAACGCCCGCAACAAAAACCTCTTTGAGAACTTAACCCCGCt > 1:718962/1‑149 (MQ=255) ccTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAGGTTAACGAAGTTAACTTCGACAAACCCAAAAACGCCCGCAACAAAATCCCCTTTGGGAACTTATCCCCGCGGc > 2:186804/1‑149 (MQ=255) cTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGACGGTG‑AACTCTATTTTGAGCTGCTGAAAGTCAACGAAGTTAACTTCGACAAACCTGAAAACGCCAGCAACAAAATCCTCTTTGAGATCTGAACCCCGCTGCa > 1:79315/1‑149 (MQ=255) aTACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt < 2:561194/149‑1 (MQ=255) tACCATTTCTGGTAAGATTCGCCGGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc < 2:131270/149‑1 (MQ=255) aCCATTCCTGGTAAGATTCGCCCGCCTAAAGAAGGTG‑AACTCTATTTTGAGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:135525/149‑1 (MQ=255) aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGATG‑AACGTTATTTTGCGCTGCTGAAAGGTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt < 1:135689/149‑1 (MQ=255) cggTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTAAGGGGTAACGGAGTTAACTTCGCCAAACCTGAAAATGCCAGCAACGAAATCTTCTTTGAGAACTTAACCCAGCTGCACGCAACATCTCTTTTGCGTATgg > 2:680707/2‑149 (MQ=255) gTAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa < 1:471966/149‑1 (MQ=255) gTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAATAAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGCGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCATATGGaa > 1:569241/1‑149 (MQ=255) tAAGATTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc > 1:275684/1‑149 (MQ=255) gATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 2:572509/1‑149 (MQ=255) aTTCGCCCTACGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACTAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg < 1:262529/149‑1 (MQ=255) aTTCGCCCGCCGAAAGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg < 1:613573/149‑1 (MQ=255) ccgccgAAAGAAGGTG‑AACTCTATTTTTCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg < 1:103708/149‑1 (MQ=255) gccgACAGAAGGTG‑AACGCTATTTTGCGCAGCTGAAAGTTAACGAAGTTAACTTCGACAACCCTGAAAACGCCCGCCCGAAAATCACCTTTGAGAGCTTAACCCCGCTTCACGCAAACACACGTCTGCGTATGGAAGGTGGTAACgggt > 2:779583/1‑147 (MQ=255) gccgAAAGAAGGTG‑AACTCTATTTTGCGATGGTGAATGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAAAAATTAACACCGATGACCACAAACTCTCGTATGCGTATGGAAAGTCGTAACCGtt > 2:503331/1‑149 (MQ=255) aaaGAAGGTG‑AACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGTGAACGTGTCTAc > 2:623897/1‑149 (MQ=255) aagaagGTT‑AACGTTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAGCCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACt < 2:550414/149‑1 (MQ=255) aagGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAATCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGaa < 1:824702/149‑1 (MQ=255) agGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTAAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAg > 1:632014/1‑149 (MQ=255) gTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGACCGGGGTAACGGTTCTACTAAAGAt > 2:251205/1‑149 (MQ=255) gTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAt > 1:536797/1‑149 (MQ=255) aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTaa < 1:556758/149‑1 (MQ=255) aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTaa < 1:649296/149‑1 (MQ=255) aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTCCGTATGGAACGTGGTAACGGTTCTACTAAAGATTaaa > 1:397862/1‑149 (MQ=255) | GATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTG‑AACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA > NC_000913_3_bme_pgi/3966594‑3966888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |