Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,235,408	Δ1 bp	intergenic (‑2175/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,235,407	0	A	.	100.0%	369.9 / NA	80	intergenic (‑2174/‑500)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (39/41);  total (39/41)

ATCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_bme_pgi/4235258‑4235550                                                                                                                                                      |                                                                                                                                                cctaaTGCCTTCGATCAATGGGGCGTGGAACCGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                 <  1:370702/147‑1 (MQ=255) aTCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGAGACGCTTCCACGCAAGGGCTTGTCGCGGATCTGCAGGGGCGGAGGAAAGCTTGa                                                                                                                                                 >  2:750748/1‑150 (MQ=255) aTCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCCCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                 <  1:750748/150‑1 (MQ=255)  tCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGat                                                                                                                                                >  2:217810/1‑149 (MQ=255)   cAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                               <  1:628335/150‑3 (MQ=255)    aaTGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATTCGCATGGGTAAGCTTGatca                                                                                                                                              >  2:918386/1‑147 (MQ=255)     aTGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatcat                                                                                                                                             <  1:600790/150‑5 (MQ=255)      tGCCCTCGATCAATGGGGGGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGTATGCGCGAAGGAAAGCTTG‑atcatc                                                                                                                                           <  1:714212/150‑1 (MQ=255)         cTTCGTTCAATGGGGAGTGGAACGGGGTAAGGAACTGGCGACGGAACTTTTGTCGGTTGTATCGGGCAAGGAAGGGGCGCGTGGTCACGACGCTTCCACGCAAGCGCTTTTTGTTTATTTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtc                                                                                                                                        <  2:301139/150‑1 (MQ=255)         cTTCGATCAATGGGGTGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtc                                                                                                                                        <  1:833329/150‑1 (MQ=255)         cTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAGGGAAAGCTTG‑ATCAtcgtc                                                                                                                                        >  1:1227434/1‑150 (MQ=255)          ttCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGCGGTCGCGGCGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtcg                                                                                                                                       >  2:853098/1‑150 (MQ=255)          ttCGATCAATGGGGCGGGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGTGTGGTCGCGAGGCTTCCACGCAAGGGCTTGTTGCGCATCTGCATGCGCGAAGGTAAGCTTG‑Atcgtcgtcg                                                                                                                                       >  1:151168/1‑150 (MQ=255)                cAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGt                                                                                                                                 <  1:1191324/150‑1 (MQ=255)                 aaTGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGCTATgca                                                                                                                                >  2:1098316/1‑148 (MQ=255)                 aaTGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTa                                                                                                                                >  2:974326/1‑150 (MQ=255)                      ggCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCg                                                                                                                           <  2:369868/150‑1 (MQ=255)                         gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGAt                                                                                                                        >  1:221477/1‑150 (MQ=255)                           ggAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTCGGCCGGATaa                                                                                                                      >  1:604292/1‑150 (MQ=255)                            gAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGGTAAg                                                                                                                     >  2:728902/1‑150 (MQ=255)                              aCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   >  1:1011583/1‑150 (MQ=255)                                tGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATGGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                 >  1:123460/1‑150 (MQ=255)                                tGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                 >  1:395810/1‑150 (MQ=255)                                 gggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                >  2:1245945/1‑150 (MQ=255)                                     aaGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                            <  2:979083/150‑1 (MQ=255)                                      aGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           <  1:217810/150‑1 (MQ=255)                                      aGGAACGGGGGACGGGACTGTTGCCGGTTGTATCGGGCAAGGAAGGGGCGGGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           <  2:556447/150‑1 (MQ=255)                                       ggccccggCCACGGATCTTGTGCCGGTTGTATCGGGCAAGGAAGGGTCGAGTGGTCGCGACGCTCCCACGCAAGGGCTTCTCGCGCATTTGCCTGCGCGCAGGAATGCTTG‑TTCATCGTCTATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                          <  1:1180222/144‑1 (MQ=255)                                       ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                          <  1:918386/150‑1 (MQ=255)                                          aCTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                 >  2:737936/1‑109 (MQ=255)                                          aCTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                 <  1:737936/109‑1 (MQ=255)                                                gACGGAACTTTTGCCGGTTGTATGGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                 <  2:123460/150‑1 (MQ=255)                                                gACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                 >  2:183559/1‑150 (MQ=255)                                                 aCGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                >  2:846190/1‑150 (MQ=255)                                                        ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGGGTTCACGCCGCATCCGGCAACCGAtc                                                                                         >  2:1189086/1‑149 (MQ=255)                                                        ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                                         >  1:468940/1‑150 (MQ=255)                                                           tGCCGGTTGTATCGGGCAAGGAAGTGGCGAGTGGTCGCGACGCTTCCACGGAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCt                                                                                      <  2:706853/150‑1 (MQ=255)                                                              cGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                                   <  2:58657/150‑1 (MQ=255)                                                               ggTTGTATCGGGCACGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAta                                                                                  >  1:1207468/1‑149 (MQ=255)                                                                gTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGTGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                                                                 <  2:763676/150‑1 (MQ=255)                                                                 ttGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCg                                                                                >  2:729081/1‑150 (MQ=255)                                                                    tATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                             >  2:1039351/1‑150 (MQ=255)                                                                      tCGGGCAAGGAAGGGGCGAGTGGGCGCGACGCGTGCACGCAAGGGCTTGTCGCGGATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGCGATGCCTGATGCGAcacg                                                                           >  2:689462/1‑150 (MQ=38)                                                                         ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGACATGAACACCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                        <  1:1098316/150‑1 (MQ=39)                                                                           gaagggaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      <  1:76630/149‑1 (MQ=255)                                                                           gaaggaaggGGGGAGTGGGCGCGACGCTGCCACGCAAGGGCGTGTCGCGCATCCGCATGCGCCAAGGAAAGCTTG‑ATCCTCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCTCATCCGGCAACCGATGCCTTATGCGACGCGGTctc                                                                      <  2:743961/149‑3 (MQ=37)                                                                           cAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      <  1:974326/150‑1 (MQ=255)                                                                            aaggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgcg                                                                     >  1:501139/1‑150 (MQ=255)                                                                             aggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                    <  1:130675/150‑1 (MQ=255)                                                                               gaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                  >  2:211727/1‑150 (MQ=255)                                                                               gaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATCCAGCACGTTATAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                  >  1:1025183/1‑150 (MQ=38)                                                                                 aggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGa                                                                                                                         >  1:1016513/1‑93 (MQ=255)                                                                                 aggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGa                                                                                                                         <  2:1016513/93‑1 (MQ=255)                                                                                 aggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGCCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                <  1:727109/150‑1 (MQ=255)                                                                                  ggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCCGCTTAt                                                               >  1:209339/1‑150 (MQ=255)                                                                                   gggCGAGTTGTCGCGAGGGTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                              <  2:1025183/150‑1 (MQ=255)                                                                                    ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATTATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTcgcatca                                                             <  2:1207468/150‑7 (MQ=38)                                                                                    ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             <  1:74508/150‑1 (MQ=255)                                                                                     gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                            <  2:395810/150‑1 (MQ=255)                                                                                         gTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                        <  1:183559/150‑1 (MQ=255)                                                                                           ggTCGCGACGCTGCCAAGCAAGGGCTTGTCGCGGATCTGCATGGGCGAAGGAAAGCTTG‑AGCAGCGTCGATATGTAGGCCGGGTAAGGCGTTCCCGCCGCAGCCGTCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                      <  2:586144/150‑1 (MQ=25)                                                                                            gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACCCGATGCCTGATGCGACGCGGTCGCGCCTTCTCAGGCCTACa                                                     >  1:793298/1‑150 (MQ=255)                                                                                            gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                     <  1:1341693/150‑1 (MQ=255)                                                                                             tCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCGGATGCGACACGGTCGCGTCTTGTCAGTGCTACAg                                                    >  1:93603/1‑150 (MQ=38)                                                                                               gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  <  1:996496/150‑1 (MQ=255)                                                                                                      ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                           >  1:581631/1‑150 (MQ=255)                                                                                                         cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTCTCAGACCTACAGGTCGATGCCGat                                        >  2:1248346/1‑150 (MQ=255)                                                                                                         cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                        <  1:757648/150‑1 (MQ=255)                                                                                                          aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                       <  2:45487/150‑1 (MQ=255)                                                                                                          aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                       >  2:1051438/1‑150 (MQ=255)                                                                                                          aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                       >  2:1092404/1‑150 (MQ=255)                                                                                                                   cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATAGGTACATCg                              >  1:168255/1‑150 (MQ=255)                                                                                                                     tGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                            <  2:1011583/150‑1 (MQ=255)                                                                                                                       tCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                          >  1:1248713/1‑150 (MQ=255)                                                                                                                         gcgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                        >  1:90297/1‑86 (MQ=255)                                                                                                                         gcgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                        <  2:90297/86‑1 (MQ=255)                                                                                                                         gcgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGTTATGTACATCGTGTTCg                        >  1:94898/1‑150 (MQ=255)                                                                                                                          cggaccggCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                       <  1:853098/143‑1 (MQ=255)                                                                                                                          cgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCTACGCGGTAGAGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                       <  1:1245945/150‑1 (MQ=255)                                                                                                                          cgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                       <  1:1039351/150‑1 (MQ=255)                                                                                                                             ggcTGCATGCGCGAAGGAAAGCTTG‑AGCATCGGCGATATGTAGGCCGGATAAGGCGTTGCCGCCGCAGCCGGCAACCGATGCTTGATGCGCCGCGGTCGCGTCTTACCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                    <  2:930337/148‑1 (MQ=25)                                                                                                                             aTCTTCATGCGCGAAGGAAAGCTCG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCTACGCGGTCGCGTCTTATCAGGTCTACAGGTCGATGCCGATATATACATCGTATTCGGCaa                    >  2:1287807/1‑150 (MQ=37)                                                                                                                             aTCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                          <  1:1357437/64‑1 (MQ=255)                                                                                                                             aTCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                          >  2:1357437/1‑64 (MQ=255)                                                                                                                             aTCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                    <  1:1346921/150‑1 (MQ=255)                                                                                                                                 gCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAGTaa                <  1:211727/150‑1 (MQ=255)                                                                                                                                  cATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCATTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaaa               >  1:1143447/1‑149 (MQ=255)                                                                                                                                       gcgAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                 <  1:532338/127‑1 (MQ=255)                                                                                                                                       gcgAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                 >  2:532338/1‑127 (MQ=255)                                                                                                                                             gAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACga    >  2:922909/1‑150 (MQ=255)                                                                                                                                               aaGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  2:1227434/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                ATCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_bme_pgi/4235258‑4235550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑