Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,233,746	A→G	intergenic (‑513/‑2161)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,233,746	0	A	G	100.0%	286.7 / NA	83	intergenic (‑513/‑2161)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/31);  total (52/31)

CTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGC  >  NC_000913_3_bme_pgi/4233603‑4233889                                                                                                                                                |                                                                                                                                                  cTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTAt                                                                                                                                             <  2:25717/150‑1 (MQ=255)       gCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTACAACCAACACAGTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCCCAATTCTCAAAATCAGAGGAGGATTGCTaa                                                                                                                                       >  2:854469/1‑150 (MQ=255)                  ttCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAc                                                                                                                            <  2:772322/150‑1 (MQ=255)                        taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAGGTCACAATTCTCAAAATCAGAGGAGTATTGATAATGGCAAGAGACGcgacg                                                                                                                      >  1:555795/1‑150 (MQ=255)                         aataaATGCTTCACTGCGCTAAGGGTTTACACCCAACATTACGCTAACGGCACTAAAACCATCACATATTTCCTTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGCAGTATCACAAATGGCAAGAGACGcgacga                                                                                                                     >  2:962685/1‑150 (MQ=255)                             aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCt                                                                                                                 >  1:665822/1‑150 (MQ=255)                               tGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTg                                                                                                               >  2:888246/1‑150 (MQ=255)                                gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATg                                                                                                                                     <  2:1273394/127‑1 (MQ=255)                                gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATg                                                                                                                                     >  1:1273394/1‑127 (MQ=255)                                 cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                                                                             <  1:105608/150‑1 (MQ=255)                                  ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAACCTTGaac                                                                                                            >  1:849515/1‑149 (MQ=255)                                   tCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGc                                                                                                           <  2:708670/150‑1 (MQ=255)                                        gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCACAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGGGACGAGGCTTGAAGCAACgg                                                                                                      >  1:371823/1‑150 (MQ=255)                                            tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGACCAGAGACGCGACGAAGCTTGAAGCAACGGTCGc                                                                                                  >  2:1041358/1‑150 (MQ=255)                                             aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCa                                                                                                 >  1:111519/1‑150 (MQ=255)                                              aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACGTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAACTCCGAGGAGTATTGCTAATGGCAAGAGACGGGACGAAGCTTGTAGCAAAGTTCGcca                                                                                                >  1:257516/1‑148 (MQ=255)                                              aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCaa                                                                                                >  1:422112/1‑150 (MQ=255)                                               gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTATTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCACAATCAGAGGAGTATTGCTAATGTCAAGAGACGCGAATAAGCTTTGAGCAACGGTCGCaaa                                                                                               >  2:1162329/1‑150 (MQ=255)                                                ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGGCGAAGGTTGCAGCAACGGTCGCAAAg                                                                                              >  1:1352376/1‑150 (MQ=255)                                                ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                              >  2:1304425/1‑150 (MQ=255)                                                 gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAAACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                             >  2:485994/1‑150 (MQ=255)                                                  tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGAAAGAGAAGCGACGCAGCTTGAACCAACGGTTACAAAACt                                                                                            >  2:271813/1‑150 (MQ=255)                                                  ttaacacTCAACATGAGTGTAACGGCACGAAAACCATCACATGTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                                                                            <  2:216060/147‑1 (MQ=255)                                                      cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaa                                                                                        <  2:1272599/150‑1 (MQ=255)                                                       acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACCATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaa                                                                                       >  1:893378/1‑150 (MQ=255)                                                       acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTCAAGCAACGGCCGCAAAGCTGaaaa                                                                                       <  1:1207316/150‑1 (MQ=255)                                                       acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTAAAGCAGCGGTCGCAAAGCTGaaaa                                                                                       >  1:881527/1‑150 (MQ=255)                                                        cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                      >  1:781282/1‑150 (MQ=255)                                                         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCACGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGACAAAg                                                                                     >  1:102299/1‑150 (MQ=255)                                                         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                     >  1:105031/1‑150 (MQ=255)                                                         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                     >  1:739365/1‑150 (MQ=255)                                                         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                     >  2:1186019/1‑150 (MQ=255)                                                           aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGACAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCa                                                                                   >  1:646001/1‑150 (MQ=255)                                                              aatACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAACCTGAAGAAGCATTg                                                                                >  2:241252/3‑150 (MQ=255)                                                              aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                             >  2:1238140/1‑137 (MQ=255)                                                              aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                             <  1:1238140/137‑1 (MQ=255)                                                              aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTGCAAGCAACGGACGCAAAGCTGAAAAAGCATTg                                                                                >  1:1122472/1‑150 (MQ=255)                                                              aTTAAGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                >  2:241229/1‑150 (MQ=255)                                                               ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg‑g                                                                              >  1:787472/1‑150 (MQ=255)                                                               ttACGCTAACGGCAATAAAACCACCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGAGGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg‑g                                                                              <  2:970508/150‑1 (MQ=255)                                                                tACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGaa                                                                                                                    >  2:989927/1‑112 (MQ=255)                                                                tACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGaa                                                                                                                    <  1:989927/112‑1 (MQ=255)                                                                tACGCTAACGGCACTAAAACCATCACAGTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGGATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg‑gg                                                                             <  2:221570/150‑1 (MQ=255)                                                                 aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGc                                                                            <  1:68194/150‑1 (MQ=255)                                                                         ggCACTAAAACCATCACATTTTTCTGTGCCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCgg                                                                    <  1:285108/150‑1 (MQ=255)                                                                         ggCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCgg                                                                    <  1:1221057/150‑1 (MQ=255)                                                                         ggCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCgg                                                                    <  2:815803/150‑1 (MQ=255)                                                                          gCACTAAAACCACCACATTTTTCTTTGTGGGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGc                                                                   <  1:1360578/150‑1 (MQ=255)                                                                                 aaCGATCACATTTTTCTGGGACTGGGGCTACAATCTTCCAAAGTCAAAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCcgcgc                                                            <  2:182128/150‑1 (MQ=255)                                                                                  aCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCcgcgcg                                                           >  2:684780/1‑150 (MQ=255)                                                                                        acaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCTACGGTCGCAAAGCTGAAAGAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATgg                                                    >  1:421492/1‑149 (MQ=255)                                                                                           tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATgcgcg                                                 >  1:852503/1‑150 (MQ=255)                                                                                               tCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCGCATAT‑CGCGCggg                                             <  1:2610/150‑2 (MQ=255)                                                                                                    gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGACAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCa                                        <  2:1379280/150‑1 (MQ=255)                                                                                                      cTGGCGCATCAATCTTCCAAAGGCACAATCCTCAAAAGCAGAGCAGTATTGCTAATGGCAAAAGACGCGACGAAGCTTGAAGCAACCGTCCCAAAGCTCAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCT‑ATATCCGCGCGGCATTCAgc                                      <  2:609455/150‑1 (MQ=255)                                                                                                       tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAgcg                                     >  2:1259500/1‑150 (MQ=255)                                                                                                        ggCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAgcgc                                    <  2:781282/150‑1 (MQ=255)                                                                                                         gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGcc                                   >  1:61779/1‑150 (MQ=255)                                                                                                           gcTACAATCTTCCAAAGTCACAATTCCCAAAACCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGa                                 <  1:281349/150‑1 (MQ=255)                                                                                                               cAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGAcccc                             >  2:1127180/1‑150 (MQ=255)                                                                                                                 aTCTTCCAAAGTCACAATTCGCCAAATCAGAGGAGGATTGCTAAGGGCAAGAGACGCGACGAAGCTTGAAGCCACGGTCGCAAAGCTGAAACAGCATTG‑GGCAGAATCGGCCCCGCGCG‑ATATACGCACGCCAGTCAGCGCCGCACccgg                           >  2:616026/1‑150 (MQ=255)                                                                                                                    ttCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggccg                        >  2:1190602/1‑150 (MQ=255)                                                                                                                       cAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                     >  1:692255/1‑150 (MQ=255)                                                                                                                        aaaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGTGCGGCAGTCAGCGCCGACCCCGGCCGGTTc                    <  2:1004464/150‑1 (MQ=255)                                                                                                                        aaaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCACTCTCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                    >  1:960225/1‑150 (MQ=255)                                                                                                                           gTCACAATTCCCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                 <  1:296111/150‑1 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAGGCACCGGTCGCAAAGCGGAAAACCCATTGAGGCCAAATCAG‑GCCTCGCT‑AGAGGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                >  2:296111/1‑150 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                 <  2:559355/149‑1 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                 >  1:559355/1‑149 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                >  2:1211474/1‑150 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                >  1:1325630/1‑150 (MQ=255)                                                                                                                            tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCCGTTCgggg                >  1:202826/1‑149 (MQ=255)                                                                                                                              acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                 <  2:541631/147‑1 (MQ=255)                                                                                                                              acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                 >  1:541631/1‑147 (MQ=255)                                                                                                                              acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCgt              >  2:1328279/1‑149 (MQ=255)                                                                                                                              acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGa              >  2:978238/1‑150 (MQ=255)                                                                                                                                  ttCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGcc                                   >  2:1280710/1‑125 (MQ=255)                                                                                                                                  ttCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGcc                                   <  1:1280710/125‑1 (MQ=255)                                                                                                                                    ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCAAAGAAGCTTGCAGCCACGGTCGCAAAGCTGCAAAAGGATCG‑GGCGGAAGCGGCCCATCGCG‑GTATGCGGGCGGCAGGAAGCGCCGACCCCGGCCGGTTCGGGGCATATTcc        >  2:475154/1‑149 (MQ=255)                                                                                                                                     tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc       >  1:70861/1‑150 (MQ=255)                                                                                                                                       aaaaGCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctc     <  2:1301150/150‑1 (MQ=255)                                                                                                                                         aaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTg   <  1:1186019/150‑1 (MQ=255)                                                                                                                                          aTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc  <  1:1390412/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  CTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTG‑GGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGC  >  NC_000913_3_bme_pgi/4233603‑4233889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑