Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,235,408	Δ1 bp	intergenic (‑2175/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,235,407	0	A	.	100.0%	419.7 / NA	92	intergenic (‑2174/‑500)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (46/46);  total (46/46)

TCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGA  >  NC_000913_3_bme_pgi/4235259‑4235548                                                                                                                                                     |                                                                                                                                              gcAATGCCTTCGAGCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                               <  2:175991/148‑1 (MQ=255)  cAATGCCTTACAACAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTCGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGat                                                                                                                                              <  1:1157499/149‑2 (MQ=255)   aaTGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCCAGTGTTCTCTACGCATCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                             <  1:647820/149‑3 (MQ=255)      gCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑atcatc                                                                                                                                         <  1:861237/149‑1 (MQ=255)         ttCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtc                                                                                                                                      >  1:157977/1‑149 (MQ=255)         ctCGATCAATGGGGCGGGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGTAAAGCTTG‑ATCAtcgtc                                                                                                                                      <  2:157605/148‑1 (MQ=255)           ggATCAATGGGGCGTGGAACCGGGCAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGa                                                                                                                                    <  1:81583/148‑1 (MQ=255)             aTCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCCTGCGCGAAGGAAAGCTTG‑ATCATCGTCGata                                                                                                                                  >  1:217753/1‑149 (MQ=255)                aaTGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCAGGCATTTGGCGGTCTCGCATCTGCATGCGCGGAGGAAAGCTTG‑ATCATCGTCGATATGt                                                                                                                               >  1:1037468/1‑149 (MQ=255)                      gCGTGGAACTGGGTAAGGATCTGGCGACTGAACTTTGGCGGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAATGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCg                                                                                                                         <  1:670461/149‑1 (MQ=255)                        gcggAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGa                                                                                                                       >  2:1222267/3‑149 (MQ=255)                        gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTCGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGa                                                                                                                       >  1:748762/1‑149 (MQ=255)                         tGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATCTGTAGGCCGGAt                                                                                                                      >  1:761403/1‑149 (MQ=255)                         tGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGAt                                                                                                                      >  1:97649/1‑149 (MQ=255)                         gtgAACGGGGTAAGGAACTGGCGCCGGAACTGTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGAt                                                                                                                      <  1:86494/147‑1 (MQ=255)                            aaCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATTGTCGATATGTAGGCCGGATAAg                                                                                                                   <  2:122732/149‑1 (MQ=255)                            aaCTGGGTAAGGAACGGGCCACGAGACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACTCTTCCACGCAATGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                   <  2:127724/149‑1 (MQ=255)                            aaCTGGGGGAGGAACTGGCGAGGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGGGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                   <  2:16003/149‑1 (MQ=255)                            aaCTGGGGAAGGAACTGGGGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                   <  2:784415/149‑1 (MQ=255)                                 ggTAAGGAACTGGCGACGGAACTTTTGTCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                              <  2:820052/149‑1 (MQ=255)                                 ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                              >  1:744873/1‑149 (MQ=255)                                    aaGGAACTGGCGACGGAACTTTTGTCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                                                                           <  1:907610/149‑1 (MQ=255)                                      ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                         >  1:996233/1‑149 (MQ=255)                                      ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                         <  1:1212605/149‑1 (MQ=255)                                      ggAACTGGCGACGGAACTTGTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                         <  1:1075508/149‑1 (MQ=255)                                       taacggtcGACGGTACTGTGGTCTGTTCTATCGGGCAAGGAAGGGGCGAGGGGTGGGGACGCTTCCACGGAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                        <  2:193114/142‑1 (MQ=255)                                       gAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                        >  1:86998/1‑149 (MQ=255)                                         aCTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                      >  1:1218264/1‑149 (MQ=255)                                              cGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGAt                                                                                                                      >  2:1185432/1‑128 (MQ=255)                                              cGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGAt                                                                                                                      <  1:1185432/128‑1 (MQ=255)                                               gACGGAACTTTTGCCGGTTGTATCGGGCGAGGAAGGCGCGAGTGGTCGCGACGCTTCCACGCAAGGGTTTGTTGTCCACCTGTATGCGCGAAGGAACGCTTG‑ATCATCGTCGTTATGTAGGCTGGCTAATGTCTGCACGCCGCATCCgg                                                                                                <  2:692150/149‑1 (MQ=255)                                               gACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                >  1:958463/1‑149 (MQ=255)                                                aCGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGGGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                               <  1:349838/149‑1 (MQ=255)                                                  ggAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                             <  1:1168110/149‑1 (MQ=255)                                                  ggAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                             >  1:728524/1‑149 (MQ=255)                                                  ggAACTTTTGCCGGTTGTAACGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                             <  2:573655/149‑1 (MQ=255)                                                  ggAACTTTGGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                             <  2:249819/149‑1 (MQ=255)                                                   gAACTTTTGCGGGGTGTGTGGGGCAAGGACGGGGGGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATTTGGAGGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                            <  2:70312/149‑1 (MQ=37)                                                       ttttGCGGCTTGTAGGGGGGAAGGAGGGGGGGAGGGGTCGGGACGCTTCCACGCAAGGGCTTGCCGGGCATCTGCATGCGCGACGGTATGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                        <  2:599021/149‑1 (MQ=25)                                                       ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                               <  1:838847/142‑1 (MQ=255)                                                       ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                               >  2:838847/1‑142 (MQ=255)                                                       ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                        >  1:507804/1‑149 (MQ=255)                                                            ccGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCCCATCCGACAACCGATGCCTg                                                                                   >  1:459609/1‑149 (MQ=255)                                                             cgcttGTATCGGGCAAGGAAGGGGTGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                  <  1:140601/146‑1 (MQ=255)                                                             cGGTTGTATCGGGCAAGGAGGGGGCGAGTGGTCGCGAGGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                  <  2:106296/149‑1 (MQ=255)                                                              ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGa                                                                                                                                    <  2:575237/98‑1 (MQ=255)                                                              ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGa                                                                                                                                    >  1:575237/1‑98 (MQ=255)                                                                   tATCGGGCAAGTAGGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGTCCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGACATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                            <  2:94743/149‑1 (MQ=38)                                                                    aTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATACCTGATGCGAcg                                                                           >  2:1010587/1‑149 (MQ=255)                                                                        ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                       <  2:774149/149‑1 (MQ=255)                                                                        ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                       >  1:1035879/1‑149 (MQ=255)                                                                         gCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                      >  1:696442/1‑149 (MQ=255)                                                                             ggaaggGGCGAGTGGTCGCGGGGCTTCCACGCAAGGGTTTGTCGGGCATGTGCATGCGCGAAGGAAAGCTTG‑ATCAGCGTCGGTATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                  <  2:584429/149‑1 (MQ=37)                                                                               aaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgt                                                                                                                                       >  2:556105/1‑78 (MQ=255)                                                                               aaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgt                                                                                                                                       <  1:556105/78‑1 (MQ=255)                                                                                   ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                            >  1:857258/1‑149 (MQ=255)                                                                                     cGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                          >  1:203322/1‑149 (MQ=255)                                                                                      gAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTGGAGGCTTATCAgg                                                         >  1:655069/1‑149 (MQ=255)                                                                                       aGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGGGACGCGGTCGCGTCTTATCAGGc                                                        >  1:422342/1‑149 (MQ=255)                                                                                          ggTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                     <  2:857258/149‑1 (MQ=255)                                                                                          ggTCGCGACGCTTCCACGCAAGGGCTTGGCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                     <  1:1248450/149‑1 (MQ=255)                                                                                              gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                 >  2:1312991/1‑149 (MQ=255)                                                                                                gtcGCTTCTTCGCAAGGGCTTGTTGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                               <  1:23028/147‑1 (MQ=25)                                                                                                gACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGCCGGACAGCTTG‑ATCATCGTCGCTATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACCCGATGCCTGATGCGCCGCGGTCGCGTTTTTTGCGGCCTACTGGTc                                               >  2:382719/1‑149 (MQ=25)                                                                                                caCGCTTGCACGCAAGGGCTTGTCGCGCATCTGCAGGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                               <  2:1272631/148‑1 (MQ=255)                                                                                                     ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATGAGGCCTACAGGTCGATGc                                          >  1:217102/1‑149 (MQ=255)                                                                                                     ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                          >  1:879801/1‑149 (MQ=255)                                                                                                       ccACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGTTGCCTGATTCGACGCGTTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                        >  2:611027/1‑149 (MQ=255)                                                                                                        cACGCAAGGGCTTGTCGCGCCTCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                       <  1:762127/149‑1 (MQ=255)                                                                                                        cACGCAAGGGCTTGTCGCGCATCTGCCTGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGACGGAAAAGGCGTTCACTCCGCATCCGGCAACCGATACCTCATGCGAACCGGCCGCGTCCTATCAGACCTACGCGTCGCTgacca                                       >  2:67960/1‑145 (MQ=255)                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                          >  1:1203411/1‑129 (MQ=255)                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                          <  2:1203411/129‑1 (MQ=255)                                                                                                          cGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                     <  2:870664/149‑1 (MQ=255)                                                                                                            cAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                     <  1:682010/99‑1 (MQ=255)                                                                                                            cAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                     >  2:682010/1‑99 (MQ=255)                                                                                                             aaGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                  <  2:696442/149‑1 (MQ=255)                                                                                                               gggCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                <  1:1298075/149‑1 (MQ=255)                                                                                                                 gCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                              >  2:821526/1‑149 (MQ=255)                                                                                                                 gCTTGTCGCGCATCAGCATGCGCGAAGGAAACCTTG‑ATCATCGACGATATGTAATCCTGATAAGGCGTTCACGCCGCATCCAGCAACCGATGCCTGATTCGACCCGGCCCCCTCTTATCAGGCCCACACATCGCTGCCGATATGTACAt                              >  2:757906/1‑149 (MQ=255)                                                                                                                  cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                             >  2:504484/1‑149 (MQ=255)                                                                                                                  cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                             <  2:372758/149‑1 (MQ=255)                                                                                                                   ttGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCCATATGTACATCg                            >  2:581120/1‑149 (MQ=255)                                                                                                                      tCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGACGACATGTAGAGCGGATAAGGAGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGGTATGTACATCGTAt                         >  1:314590/1‑149 (MQ=37)                                                                                                                        gcgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                       >  1:1164914/1‑149 (MQ=255)                                                                                                                         cgcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAAAGCGAGAGGTCGATGCCGATATGTACATCGTATTCg                      <  1:1312991/149‑1 (MQ=37)                                                                                                                          gcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                >  1:1077748/1‑122 (MQ=255)                                                                                                                          gcATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                <  2:1077748/122‑1 (MQ=255)                                                                                                                             tCTGGATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                  <  1:611027/149‑1 (MQ=255)                                                                                                                                gCATGCGCGAAGGAAAGCTTG‑ATCATCGCAGATCTCGTGTTCTGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTGCATCGTATTCGGCAATTa               >  1:652657/1‑149 (MQ=21)                                                                                                                                 cATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa              <  1:504484/149‑1 (MQ=255)                                                                                                                                   ggcgcGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa            <  2:652657/148‑1 (MQ=255)                                                                                                                                    gcgcGTAGGAAAGCTTG‑ATCATCGTTGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc           <  1:1004399/149‑1 (MQ=255)                                                                                                                                       cgAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa        >  1:497715/1‑149 (MQ=255)                                                                                                                                        gAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg       >  2:67164/1‑149 (MQ=255)                                                                                                                                            gAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACg   >  2:1176085/1‑149 (MQ=255)                                                                                                                                             aaaGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCActa  >  1:400768/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                              TCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGA  >  NC_000913_3_bme_pgi/4235259‑4235548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑