Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	4,015,910	Δ1 bp	coding (260/816 nt)	ysgA ←	putative carboxymethylenebutenolidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	4,015,910	0	A	.	100.0%	312.9 / NA	64	coding (260/816 nt)	ysgA	putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (36/28);  total (36/28)

GCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATC  >  NC_000913_3_KO_pgi/4015765‑4016052                                                                                                                                                  |                                                                                                                                               gccgTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGcc                                                                                                                                           <  3:397787/150‑1 (MQ=255)    gTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGa                                                                                                                                        <  3:22156/150‑1 (MQ=255)       gCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAc                                                                                                                                     <  1:639414/150‑1 (MQ=255)                ccAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgc                                                                                                                            <  2:475598/150‑1 (MQ=255)                  aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                          >  1:653511/1‑150 (MQ=255)                  aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                          >  3:415095/1‑150 (MQ=255)                     tGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAg                                                                                                                       <  4:83127/150‑1 (MQ=255)                     tGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAg                                                                                                                       >  1:192522/1‑150 (MQ=255)                                   agaTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATa                                                                                                                                      <  1:248340/121‑1 (MQ=255)                                   agaTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATa                                                                                                                                      >  2:248340/1‑121 (MQ=255)                                    gaTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGTGGATATCGGCAAAATCCTTCTGATCGACTACACGGAAGTAAAGATCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTc                                                                                                        >  2:573536/1‑150 (MQ=255)                                      tCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGAGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgc                                                                                                      <  2:656104/150‑1 (MQ=255)                                       cGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgcg                                                                                                     <  3:58189/150‑1 (MQ=255)                                       cGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgcg                                                                                                     >  3:21980/1‑150 (MQ=255)                                       cGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgcg                                                                                                     <  2:420187/150‑1 (MQ=255)                                        ggCCAGCACCTGCGAGTCGGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGCTCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAAATACCCCCGCAGCGCCAGACGGCGACAAATGTCGCgg                                                                                                    >  2:60105/1‑150 (MQ=255)                                         gCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGa                                                                                                   <  2:300499/150‑1 (MQ=255)                                          ccAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGat                                                                                                  >  1:92801/1‑150 (MQ=255)                                                   gCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATg                                                                                         <  3:319794/150‑1 (MQ=255)                                                          aGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCa                                                                                  >  3:153951/1‑150 (MQ=255)                                                                      tACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGc                                                                      <  4:86952/150‑1 (MQ=255)                                                                       aCCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCa                                                                     >  3:82674/1‑150 (MQ=255)                                                                        ccAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCaaa                                                                                <  4:93512/138‑1 (MQ=255)                                                                        ccAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCaaa                                                                                >  3:93512/1‑138 (MQ=255)                                                                         cAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCGCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACt                                                                   >  2:484619/1‑150 (MQ=255)                                                                          aGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTa                                                                  >  4:202906/1‑150 (MQ=255)                                                                               gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTcc                                                                                                                               <  4:291061/84‑1 (MQ=255)                                                                               gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTcc                                                                                                                               >  3:291061/1‑84 (MQ=255)                                                                               gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCt                                                                        >  2:65383/1‑139 (MQ=255)                                                                               gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCt                                                                        <  1:65383/139‑1 (MQ=255)                                                                                 tAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGAc                                                           <  1:651020/150‑1 (MQ=255)                                                                                  aaGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                          >  1:569/1‑134 (MQ=255)                                                                                  aaGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                          <  2:569/134‑1 (MQ=255)                                                                                      aaCGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTg                                                      <  1:461718/150‑1 (MQ=255)                                                                                         gTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCa                                                   >  4:192462/1‑150 (MQ=255)                                                                                         gTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCa                                                   >  2:505446/1‑150 (MQ=255)                                                                                              gATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTggg                                              <  3:292296/150‑1 (MQ=255)                                                                                                 atCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCa                                           <  1:505446/150‑1 (MQ=255)                                                                                                 atCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCa                                           >  3:112978/1‑150 (MQ=255)                                                                                                   cGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGCTCCGGTGTG‑TAGCAAGATACCCCTCCAGCGCCAGACGGCGCCAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTCCAATCACCAATGGCAGTGGGCCATc                                         >  2:62464/1‑150 (MQ=255)                                                                                                    ggCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCg                                        >  4:425882/1‑150 (MQ=255)                                                                                                    ggCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCg                                        >  3:167234/1‑150 (MQ=255)                                                                                                     gCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGTCAGTGGGCCATCgc                                       >  2:156106/1‑150 (MQ=255)                                                                                                         aaTCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttg                                   <  1:454084/150‑1 (MQ=255)                                                                                                           tCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgct                                 >  2:623797/1‑150 (MQ=255)                                                                                                               tCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTgg                             >  3:428863/1‑150 (MQ=255)                                                                                                                      gCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCa                      <  4:415095/150‑1 (MQ=255)                                                                                                                       ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACaa                                                                                                             >  3:371217/1‑62 (MQ=255)                                                                                                                       ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACaa                                                                                                             <  4:371217/62‑1 (MQ=255)                                                                                                                        cTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATg                    <  4:428863/150‑1 (MQ=255)                                                                                                                         ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTAc                                                                 <  1:131083/104‑1 (MQ=255)                                                                                                                         ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTAc                                                                 >  2:131083/1‑104 (MQ=255)                                                                                                                         ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATgg                   >  1:27456/1‑150 (MQ=255)                                                                                                                         ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATgg                   >  2:621550/1‑150 (MQ=255)                                                                                                                         ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATgg                   >  4:407194/1‑150 (MQ=255)                                                                                                                            gcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGa                                                                                                                      >  3:89462/1‑48 (MQ=255)                                                                                                                            gcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGa                                                                                                                      <  4:89462/48‑1 (MQ=255)                                                                                                                            gcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTaa                >  2:484401/1‑150 (MQ=255)                                                                                                                               gAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCa             <  3:157819/150‑1 (MQ=255)                                                                                                                                   tAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCa         >  3:325385/1‑150 (MQ=255)                                                                                                                                     aaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTAGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATg       >  2:395638/1‑150 (MQ=255)                                                                                                                                      aGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGt      >  3:235289/1‑150 (MQ=255)                                                                                                                                       gTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGtt     >  4:310630/1‑150 (MQ=255)                                                                                                                                          cAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATc  <  4:82674/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               GCCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATC  >  NC_000913_3_KO_pgi/4015765‑4016052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑