| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_pgi | 3,815,812 | G→T | intergenic (‑44/+22) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_pgi | 3,815,812 | 0 | G | T | 93.3% | 38.3 / ‑6.6 | 15 | intergenic (‑44/+22) | pyrE/rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (5/9); minor base C (0/1); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA > NC_000913_3_vch_pgi/3815666‑3815957 | agaaATAGGTGCTTTCGCGCTCGGAGTTAAGCTTAAACCTCACAAACTTTAACACCTGTATGCTAAGCGCAAATACAATAAACTGGCGCTGATA‑TGGTTTCATTCCTTCGCTCCTGATCTTACTTTTATACAGAC‑AAAAAAAAGGCTAc < 2:88843/149‑1 (MQ=255) tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTccc > 2:180627/1‑147 (MQ=255) ttCAAGAAACTGGCGCTAATC‑TGGTTTCATGACTTCCCTGCCGATCTTACATGTCTACAGAA‑AAAAGAAATGCTACTCATCAGTCTTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg < 2:156718/149‑1 (MQ=255) cAATAATCTGGCTCTGGTAGAGGTTTC‑GGCCTTCGTCCCTCCTCTTACTTTTACACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc < 2:52477/149‑1 (MQ=255) aaCTGGCGCGGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGCCGCCTTAAAAATCAGGTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCagag < 1:222988/149‑1 (MQ=255) gcgcTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa < 1:259047/149‑1 (MQ=255) gcgcTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCGTCAGTCGCCTTAAAAATCTGTTTGCCAGCGCCGCCTTCTGCGTCGGTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac > 2:201178/1‑149 (MQ=255) gcgcTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCCTCGCCGCCTTCTACGTCGCTACAATGGATTCGCTTCCCCTCGGGCCAGAGCcaac > 2:201207/1‑149 (MQ=255) gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCa < 1:3375/68‑1 (MQ=255) gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCa > 2:3375/1‑68 (MQ=255) cTTCGCTCCTCATCTTACTTTTCTACATAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg < 1:70471/149‑1 (MQ=255) cGCTCCTGATCTTACCTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt < 1:135203/149‑1 (MQ=255) cctcATCTTACTTTTCGACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGTCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGTTGAAc < 2:224644/149‑1 (MQ=255) tCTTACTTTTCTACAGAC‑AAAAAAAAGGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAAGAGTAGATCTTCATGGGTGAACGGCtg > 2:78234/1‑148 (MQ=255) ggCCACTCATCAGCCGCCTCAAAAATCCGTTTGCCAGCGCCCCCTGCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGACCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCCTCCCCTGCACTTCaa < 2:156081/149‑1 (MQ=255) | AGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATA‑TGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA > NC_000913_3_vch_pgi/3815666‑3815957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |