Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,817,170	C→A	E112* (GAA→TAA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,817,170	0	C	A	100.0%	236.8 / NA	76	E112* (GAA→TAA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/44);  total (32/44)

TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_hsa_tpiA/2817026‑2817315                                                                                                                                                 |                                                                                                                                                   tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGCTGGCGCTTTAACTc                                                                                                                                                >  2:65501/1‑149 (MQ=255)    gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGCTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcccc                                                                                                                                             >  2:508391/1‑149 (MQ=255)         aCGCCCATACGCTGCGCGCACAACGAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCGTCAGCGAGGGCGCGTTAACTCCCCGTTcc                                                                                                                                        >  1:483361/1‑149 (MQ=255)         aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                        <  2:598279/149‑1 (MQ=255)              cATAGGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAg                                                                                                                                   <  2:798017/149‑1 (MQ=255)               aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGc                                                                                                                                  >  2:1280741/1‑149 (MQ=255)                   gCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                              <  1:1225415/149‑1 (MQ=255)                          gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                       <  1:561876/149‑1 (MQ=255)                           cacaACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacg                                                                                                                      >  1:26253/1‑149 (MQ=255)                            acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAAc                                                                                                                                                  <  2:527523/120‑1 (MQ=255)                              aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                   >  1:1115283/1‑149 (MQ=255)                              aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                   >  1:692776/1‑149 (MQ=255)                                caaAAATGTGCCTGGCGCGGGTTTCTGGTGTTTGACGTAATCGGCAGCGACGACGGCGCAAAAACACTGGCGTAATCCCAGGGGCGCCAGCAATGCCTCTGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                 <  1:419119/149‑1 (MQ=255)                                 aaaaaTGTGTCTGGCGCGGGTTTATGGTGATTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGACAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCATACAGCCATTGGGCGACGACCATg                                                                                                                >  2:1297356/1‑149 (MQ=255)                                        tgtCTGGCGCGGGTTTATGGTGTTTGACGTGATTGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACt‑t                                                                                                        <  2:795574/149‑1 (MQ=255)                                           cTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTc                                                                                                     <  2:1333257/149‑1 (MQ=255)                                            tGGCGCGTGTTTCTGGTGTTTCGGGTGATCGGCAGCGACGACGGCGTCAGAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCGCAGCGATGGTGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTca                                                                                                    <  2:88324/149‑1 (MQ=255)                                            tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATATTGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTca                                                                                                    <  1:1233828/149‑1 (MQ=255)                                            tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCGTGCCAACT‑TTTca                                                                                                    >  2:927547/1‑149 (MQ=255)                                            tGGCGCGGGTTTATGGTGTGTGACGGGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTca                                                                                                    <  2:248998/149‑1 (MQ=255)                                             ggggcgGGTTTATGGGGTTGGACGGGATCGGCGGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTcac                                                                                                   <  2:196265/146‑1 (MQ=255)                                              gcgcGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTcacc                                                                                                  >  2:278636/1‑149 (MQ=255)                                               cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTcacca                                                                                                 >  1:133523/1‑149 (MQ=255)                                               cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTcacca                                                                                                 <  2:585356/149‑1 (MQ=255)                                                  gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAGTGGGCGACGACCATGCCAACT‑TTTCACCACAt                                                                                              >  1:484070/1‑149 (MQ=255)                                                   ggTTTATGGTGATTGAAGTGATCGGCAGCGACGACGGCGTCAAAAGAATCGCGTAATCCCAGCTGCGCCAGCAATGCCTCAGTGAAGGCGCTTTAACTCCACGTTCCTACAGCCATTGGGCCACGACCATGCCAACT‑TTTcaagatcgg                                                                                             >  2:1293128/1‑142 (MQ=255)                                                    gTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATca                                                                                            >  1:1332653/1‑149 (MQ=255)                                                    gTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTAACCACATca                                                                                            >  1:1332569/1‑149 (MQ=255)                                                      ttATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACTTcgac                                                                                          <  2:896978/149‑1 (MQ=255)                                                         tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATNCNANCN‑TNTCACCACATcaccacg                                                                                       >  1:790401/1‑147 (MQ=255)                                                          ggggtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAAGGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAaga                                                                                      <  1:278636/146‑1 (MQ=255)                                                           gtgtgtGACGGGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAagag                                                                                     <  2:688537/149‑1 (MQ=255)                                                            tgtTTGACGTGATCGGCAGCGACGCCGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTAAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAATT‑GTTCACCACAGCAACAAgggg                                                                                    >  1:462385/1‑149 (MQ=255)                                                            tgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAgg                                                                                    <  2:133523/149‑1 (MQ=255)                                                              tttGACGTGATCGGCAGCGACGACTGCGTCAAAATAATTGCGTAATCCCAGGTGCGCCAGCAACGCCTCATAGATGGCACGTGAACTTCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGaa                                                                                  <  2:1231772/149‑1 (MQ=255)                                                              tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGaa                                                                                  >  2:1070601/1‑149 (MQ=255)                                                               ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAg                                                                                 <  2:26253/149‑1 (MQ=255)                                                                 gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCg                                                                               >  1:1250295/1‑149 (MQ=255)                                                                 gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAACGATGGCGCTTTAACTCCACGTTACCAAAGCCATTTGGCGACAACCATG‑CAACTGTGTCACCACATCAACAAGAGGAAGCg                                                                               >  2:1302856/1‑149 (MQ=255)                                                                 gACGGGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCg                                                                               <  2:1042424/149‑1 (MQ=255)                                                                  aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCgg                                                                              <  1:944556/149‑1 (MQ=255)                                                                    gggATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAAGCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGtt                                                                            <  2:1344668/147‑1 (MQ=255)                                                                     tGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTc                                                                           >  1:4156/1‑149 (MQ=255)                                                                        tCGGGAGTGATGACGGCGTGAAAATAGCGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                        <  2:915946/149‑1 (MQ=255)                                                                        tCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                        <  1:1346201/149‑1 (MQ=255)                                                                         cGGCAGCGGCGACGGCGTTAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                       <  1:301479/149‑1 (MQ=255)                                                                            cAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTa                                                                    >  1:135946/1‑149 (MQ=255)                                                                              gCGACGACGGCGTCAAAATAATGGCGTAAGCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCCCTTTAACCCCCCGTCCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATc                                                                  <  2:599201/149‑1 (MQ=255)                                                                                gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca                                                                <  2:692776/149‑1 (MQ=255)                                                                                 acgacgGCGTCGAAATAATGGCGTAATCCCGGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                               <  1:499685/149‑1 (MQ=255)                                                                                     cgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCagcagc                                                           >  2:597956/1‑149 (MQ=255)                                                                                        cggcAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATa                                                        <  1:17403/146‑1 (MQ=255)                                                                                             aaaGAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                                   <  2:565185/149‑1 (MQ=255)                                                                                               ataatTGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa                                                 <  1:1344225/149‑1 (MQ=255)                                                                                               ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                    >  2:300663/1‑67 (MQ=255)                                                                                               ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                    <  1:300663/67‑1 (MQ=255)                                                                                                   tGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGc                                             <  1:508391/149‑1 (MQ=255)                                                                                                         aaTCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCGTTTCTACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                       <  2:941536/149‑1 (MQ=255)                                                                                                           tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttt                                     <  2:747673/149‑1 (MQ=255)                                                                                                           tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttt                                     <  2:747674/149‑1 (MQ=255)                                                                                                             ccAGTTGCGCCAGGAATGCCTGAGGGATGGCGCTTTAACTCGCCGTTCGTATAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt                                   <  2:790401/149‑1 (MQ=255)                                                                                                                 gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTccc                               >  2:137662/1‑147 (MQ=255)                                                                                                                       cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTa                         >  2:572356/1‑149 (MQ=255)                                                                                                                              gCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATg                  <  1:572356/149‑1 (MQ=255)                                                                                                                                cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg                >  1:1293187/1‑149 (MQ=255)                                                                                                                                 tccgCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGATGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               <  2:362964/146‑1 (MQ=255)                                                                                                                                    gCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc            <  1:109266/149‑1 (MQ=255)                                                                                                                                     cGATGGACCTTTAACACCCCGCTCCCACAGCCATTGGGCGACGGCCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCACCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTCACCCATGCgggccc           >  2:494649/1‑146 (MQ=255)                                                                                                                                      gATGGCGCTTTAACTCCCTGTTCCTACAGCCATTGGGCGACGATCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCga          <  1:823388/149‑1 (MQ=255)                                                                                                                                      gATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGAGGACCATGCCAACT‑TTTCACCAAATCAACAAGAGGAAGCGGTTCGACGCTATCAAGCAGCGTACTTCTTACTGCTTCTGGTTTTTCACGCGATAACGCATGCGGGTCga          >  1:285834/1‑149 (MQ=255)                                                                                                                                        tGGCGGTTTAACGCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCgaga        <  2:788759/149‑1 (MQ=255)                                                                                                                                         ggCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCCACGACCATGCCAACT‑TTTCACCACATCAACACGAGGAAGCGGTTCGACGCTATCCACCAGCATACTTCTTACTCCTTCTGTATTTTCACGCGCTAACGCATGCGGGTCGAGAt       >  2:1366635/1‑149 (MQ=255)                                                                                                                                           cgcTTTAACTCCCCTTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAATAGGAAGCCGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg     <  1:303208/149‑1 (MQ=255)                                                                                                                                           cgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCCGCATACTTCTTCCTGCTTCTGTTTTTTCACGCGCTACCGCATGCGGGTCGAGATCg     >  1:724003/1‑149 (MQ=255)                                                                                                                                           cgcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg     <  1:1385450/149‑1 (MQ=255)                                                                                                                                              tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTATTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc  >  2:26420/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACT‑TTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_hsa_tpiA/2817026‑2817315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑