Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,775,562	Δ1 bp	intergenic (+118/‑25)	aroD → / → ydiF	3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,775,559	0	A	.	98.8%	379.0 / ‑2.7	80	intergenic (+115/‑28)	aroD/ydiF	3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (36/43);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTGGCGGCG  >  NC_000913_3_hsa_tpiA/1775413‑1775701                                                                                                                                                   |                                                                                                                                               gcACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaat                                                                                                                                             <  2:367808/150‑2 (MQ=255)   aCGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCCCTAATTCTCCATTTGCCGTAGGGaaatcc                                                                                                                                           >  2:158751/1‑147 (MQ=255)     ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATcaca                                                                                                                                        <  1:595443/150‑1 (MQ=255)     ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATcaca                                                                                                                                        >  4:172764/1‑150 (MQ=255)       tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATc                                                                                                                                      >  2:509111/1‑150 (MQ=255)        aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCt                                                                                                                                     <  1:753238/150‑1 (MQ=255)            aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAAt                                                                                                                                 >  2:304344/1‑150 (MQ=255)              tATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCa                                                                                                                               <  2:523929/150‑1 (MQ=255)               aTTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCACTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAg                                                                                                                              <  3:344001/150‑1 (MQ=255)                 tttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGa                                                                                                                            >  1:901654/1‑150 (MQ=255)                        cAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACa                                                                                                                     >  3:86651/1‑150 (MQ=255)                        cAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACa                                                                                                                     >  3:534610/1‑150 (MQ=255)                          ggCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTA‑AAAAAATCCAATCTGAATCAGGAATTAACAAt                                                                                                                   <  4:506182/150‑1 (MQ=255)                                aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAAcc                                                                                                             >  2:346114/1‑150 (MQ=255)                                  cAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGACTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AACTCACATCTGAATCAGGAATTAACAATGAAACCTg                                                                                                           >  1:944897/1‑150 (MQ=255)                                   aataatATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAGCAATGAAACCTGt                                                                                                          >  4:597877/1‑150 (MQ=255)                                   aataatATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                                                                          <  1:1124357/150‑1 (MQ=255)                                   aataatATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                                                                          <  4:520283/150‑1 (MQ=255)                                     taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaa                                                                                                        >  1:501393/1‑150 (MQ=255)                                      aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                                                                       >  2:141384/1‑150 (MQ=255)                                        tatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                                                                     <  1:304344/150‑1 (MQ=255)                                             cggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                                                                                <  4:466227/150‑1 (MQ=255)                                               gcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCg                                                                                              >  2:662719/1‑150 (MQ=255)                                                 gggAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                                                                            <  1:509111/150‑1 (MQ=255)                                                   gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAtt                                                                                          >  3:590512/1‑150 (MQ=255)                                                     acacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAaccac                                                                                                  >  4:298316/1‑140 (MQ=255)                                                     acacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAaccac                                                                                                  <  3:298316/140‑1 (MQ=255)                                                     acacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTaa                                                                                        <  4:164659/150‑1 (MQ=255)                                                      cacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                                                                       >  2:583573/1‑150 (MQ=255)                                                         ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGa                                                                                    >  2:96342/1‑150 (MQ=255)                                                         ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGa                                                                                    <  2:859056/150‑1 (MQ=255)                                                          cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGAc                                                                                   >  1:950286/1‑150 (MQ=255)                                                            ccccGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGa                                                                                 >  2:30908/1‑150 (MQ=255)                                                               cgccgAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTg                                                                              <  4:86651/150‑1 (MQ=255)                                                                   gAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                                                                          <  2:656788/150‑1 (MQ=255)                                                                        aaaaaaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTg                                                                     >  1:651005/1‑150 (MQ=255)                                                                         aaaaaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGt                                                                    <  4:649478/150‑1 (MQ=255)                                                                         aaaaaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCACTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGt                                                                    >  2:740431/1‑150 (MQ=255)                                                                           aaaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg                                                                  >  2:129028/1‑150 (MQ=255)                                                                               atatTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcac                                                              <  4:426423/150‑1 (MQ=255)                                                                                tatTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca                                                             <  3:597877/150‑1 (MQ=255)                                                                                tatTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca                                                             <  1:994198/150‑1 (MQ=255)                                                                                 atTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAg                                                            <  4:555981/150‑1 (MQ=255)                                                                                     attCGTATTTAATATAGATATTTCGTGAGTCTCTGTGCGCTCATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAACCCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGGCGGCACAGGAAg                                                        <  2:1061126/148‑1 (MQ=255)                                                                                       tCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAAc                                                                                                                      <  1:821119/86‑1 (MQ=255)                                                                                       tCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAAc                                                                                                                      >  2:821119/1‑86 (MQ=255)                                                                                       tCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg                                                      >  2:299671/1‑150 (MQ=255)                                                                                          tATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                                                                    >  1:716510/1‑101 (MQ=255)                                                                                          tATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                                                                    <  2:716510/101‑1 (MQ=255)                                                                                            tttAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGaa                                                 <  1:374283/150‑1 (MQ=255)                                                                                              taataaAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAtt                                               <  1:248914/150‑1 (MQ=255)                                                                                              taataaAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAAtt                                               <  4:590512/150‑1 (MQ=255)                                                                                               aataaAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                                                                       <  1:149855/109‑1 (MQ=255)                                                                                               aataaAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                                                                       >  2:149855/1‑109 (MQ=255)                                                                                                 taaAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATtata                                            <  2:321220/150‑1 (MQ=255)                                                                                                 taaAAATATTTCGTGAGGCTCTGTGCGCTAATTCTCCATTTAGCGCAGGG‑AAAGCACATCTGAGTCAGGAATGAACAATGAAACCTGTAAAACCACCTCGTAGTAATGGACGAGTGCCGGTCCTGGCGGCACAGGGAGCGGTGAATtata                                            >  4:357206/1‑150 (MQ=255)                                                                                                 taaAAAGATTTCGTGAGTCTGTGTGCGCTAATTGTCCTTTTGGCGTAGGG‑AAATCACATATGAAGCAGGAATTAACAATGAAACCTTTAAAACCACCTCGTATTACTGGACGAGTGCCGGTCTTGTTGGCACAGGCAGGGGTGAAGtata                                            <  2:27922/150‑1 (MQ=255)                                                                                                          ttCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGc                                                                             <  1:1129988/108‑1 (MQ=255)                                                                                                          ttCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGc                                                                             >  2:1129988/1‑108 (MQ=255)                                                                                                          ttCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCcgacg                                   <  2:832375/150‑1 (MQ=255)                                                                                                           tCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaat                                                                                                                                             >  3:191190/1‑43 (MQ=255)                                                                                                           tCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaat                                                                                                                                             <  4:191190/44‑2 (MQ=255)                                                                                                           tCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCcgacga                                  <  3:172764/150‑1 (MQ=255)                                                                                                              tGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGc                               <  1:141384/150‑1 (MQ=255)                                                                                                                 gTCTCTGTGCGCTAATTCTCCATTTGGCGTCGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAac                            >  2:156463/1‑150 (MQ=255)                                                                                                                      tgtgCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCacatccat                       >  2:85537/1‑143 (MQ=255)                                                                                                                      tgtgCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACttt                       <  1:346114/150‑1 (MQ=255)                                                                                                                       gtgCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTGTATTCCCGACGAAGCAACACTTtg                      <  3:196074/150‑1 (MQ=255)                                                                                                                        tgCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTtgt                     >  1:781/1‑150 (MQ=255)                                                                                                                        tgCGCTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTtgt                     >  1:578613/1‑150 (MQ=255)                                                                                                                        tgCGCTAATTCTCCATTTGGCCTAGCG‑AACTCCCATCTGCATCAGGCATTCCCACTGCAAACTGTACTCCCCCCTCGTATTAACGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTtgt                     <  3:357206/150‑1 (MQ=255)                                                                                                                         gcgcTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTtgtg                    <  2:715950/150‑1 (MQ=255)                                                                                                                          cgcTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTtgtgt                   <  2:651005/150‑1 (MQ=255)                                                                                                                            cTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTgtgtgt                 <  2:901654/150‑1 (MQ=255)                                                                                                                            cTAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTgtgtgt                 <  3:227306/150‑1 (MQ=255)                                                                                                                             tAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGtt                <  4:592696/150‑1 (MQ=255)                                                                                                                             tAATTCTCCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACAAAGCAACACTTTGTGTGtt                >  2:1132964/1‑150 (MQ=255)                                                                                                                                   tcCATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGgcgc          >  4:283462/1‑150 (MQ=255)                                                                                                                                    ccATTTGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCt         >  1:550932/1‑150 (MQ=255)                                                                                                                                       tttGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTggc      >  2:186442/1‑150 (MQ=255)                                                                                                                                         tGGCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTggcgg    <  2:550932/150‑1 (MQ=255)                                                                                                                                          ggCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTggcggc   >  1:668577/1‑150 (MQ=255)                                                                                                                                          ggCGTAGGG‑AAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTggcggc   <  2:950286/150‑1 (MQ=255)                                                                                                                                           gCGTAGGG‑AAATCACATCTGAACCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATCAATGGATGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTAAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTGgcggcg  <  2:1146167/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               GCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGTGAATTATATTCCCGACGAAGCAACACTTTGTGTGTTAGGCGCTGGCGGCG  >  NC_000913_3_hsa_tpiA/1775413‑1775701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑