| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,544,021 | 1 | . | A | 79.0% | 40.2 / 8.0 | 19 | coding (40/1389 nt) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base A (9/6); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| * | NC_000913_3_pae_tpiA | 1,544,022 | 0 | C | A | 94.7% | 46.6 / NA | 19 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (11/7); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATG > NC_000913_3_pae_tpiA/1543896‑1544146 ||| tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATACCGACTATTTTtc > 2:76591/1‑149 (MQ=255) tAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCAtttttc > 2:236207/1‑147 (MQ=255) tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGc > 1:503531/1‑149 (MQ=255) cAGAAACAGAAGGTAAGAAGTAAACAACTGGCTGATATCCAGAGATTTCTTCGAGTAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAATAACG‑C‑ATAA‑A‑GATAACGACTCTGTTTCTCATTTTGCAGTGCCa < 2:88812/149‑1 (MQ=255) agaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCGGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCCGTGCCATATTGTTCCTCACAt > 2:403124/1‑149 (MQ=255) agcaGACAACCGACTGATATCCAGCGATTTCTTCGACTAATATGTGTTCCTTTATTTTCTCAGCAGGCCGGATTTTCCGGTTTCCAGTCGCG‑A‑A‑AAGA‑GATAACCACTATTTTGCTCATTTTGCAGTGCCATATTGTTCCTCCCGTGct < 2:482100/146‑2 (MQ=255) aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTACTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg < 1:434276/149‑1 (MQ=255) tgactgaTATCAAGATGGTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCCCCCATTGGTa > 2:324598/1‑149 (MQ=255) gaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACaa < 1:245115/149‑1 (MQ=255) gttgAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTTCAGTGCCATGTTGTTCCTCACAGGCACACATCGGTAATGC‑AAAAAACACAAAACACg > 2:138321/4‑149 (MQ=255) ctacagaaaTAGGTTTTCCTTTATTTTCCCAGAAGGCCGAATTTTCTGGTTTGCAGTCGCG‑A‑A‑AA‑AGGATAACGACTATTTTTCTCATTTTGCAGTGCCATCTTGTTCCTCACATTCACACATT‑GTTATGAGAAAAAAGACTAAACACg > 2:114638/8‑149 (MQ=255) cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCAATATTGTTCCTCACATGCACACATTGGTAGTGA‑AAAAACAACAAAAAACGAGGTAAGGCGCAATGGcc > 2:360296/1‑149 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACaaaa < 2:442770/126‑1 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACaaaa > 1:442770/1‑126 (MQ=255) tttATTTTCCCAAGAGCCTGGATTTTCGGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATGTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa < 1:68960/149‑1 (MQ=255) tCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatta < 1:180524/149‑1 (MQ=255) aGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGGCTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAGGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa < 1:82073/149‑1 (MQ=255) gCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAgg > 1:330921/1‑149 (MQ=255) ggATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATg > 2:442548/1‑149 (MQ=255) ||| TGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AA‑A‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGA‑AAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATG > NC_000913_3_pae_tpiA/1543896‑1544146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |