| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 2,391,417 | C→G | 8.3% | W30S (TGG→TCG) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 2,391,417 | 0 | C | G | 8.3% | 131.5 / 4.6 | 49 | W30S (TGG→TCG) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base C (20/24); new base G (2/2); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01 | |||||||||||
GCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGC > NC_000913_3_pae_tpiA/2391271‑2391561 | gCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTACCGTCCATAGCGCCCGCCTGGCCAACAAACCAGACCGAAACCAGCGCCGCGTGAAGCCCAATAACGGAGAGCGTAGCGTTCAGCAAATGCTAGCGTcccccc > 2:244584/1‑147 (MQ=255) gggggaaaGCATGGCGAAACTATCAACGCGCATCAGCGGCGGAACGGCCATAGCGCCCGCCTGGCCAACAAACCATAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCaa < 2:32706/144‑1 (MQ=255) tgtAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATg < 1:610963/149‑1 (MQ=255) aaGCATGGCGAAACCATCAACGGGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgaga < 1:398083/149‑1 (MQ=255) aGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttc < 1:729532/149‑7 (MQ=255) aaaCCATCAACGCGCAGCAGCTGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAac < 2:386121/149‑1 (MQ=255) aCCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCTCCACGCACTGGAGAGCATC‑ACAac‑ca > 2:471917/1‑149 (MQ=255) cATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAac‑cacc > 2:360834/1‑149 (MQ=255) tCAACGCCCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAGACCGAGCGCAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAac‑caccac < 2:573821/149‑1 (MQ=255) cgcgCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCATc > 2:435715/1‑149 (MQ=255) gcATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTAAGGAAATGATTGCGTCGCAACGCAAGCGAGAGCATC‑ACAAC‑CACGCACGTCAAg > 2:703089/1‑149 (MQ=255) aGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCcgac < 1:536166/149‑1 (MQ=255) ggcgTAACGTCCACAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGAt < 1:587132/149‑1 (MQ=255) cgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCa < 2:463715/149‑1 (MQ=255) gtacATACCGCCCACCTGGCCACCAAACCAGAGCGAAACCAGCCCCGCGTTACACCCAATAACCGAGAGCGTAGCAGTGAGGCAATGACTGCGTCTCCACCCAATGGAAAGCCAC‑ACACC‑ACCCACCGAAAAGACGACCACGAGCAACg > 2:547785/4‑149 (MQ=255) ccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGGGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCACCCACGGTAGCAGAGca > 2:820204/1‑148 (MQ=255) gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGAAATGGTGAGCATC‑ACAAC‑CAACACCGTCAAGCCACCGATCAGCAACGGTAGCAGGGCGAt > 2:530346/1‑149 (MQ=255) tGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAg < 1:295735/149‑1 (MQ=255) gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt > 2:846019/1‑149 (MQ=255) gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGGTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCCACGGTGGAAGGGCGATCAGGt > 2:845982/1‑149 (MQ=255) aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGGAATGGAGAGCATG‑AGAAG‑CACCACCGTCAAGCCGATGATCAGCTACGGTAGCAGTGCGGTCAGGtttt > 2:154257/1‑149 (MQ=255) caaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATATCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg < 2:355390/148‑1 (MQ=255) agCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAt < 2:766940/149‑1 (MQ=255) gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCAACGTCAAGCCGACGATCAGCAACGGTAGGAGGCAGATCAAGTTTGGGGGAggaatagt > 2:350851/1‑142 (MQ=255) aaCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACCAACAGCAAAGATAGCAATACAATCAGAATTAGTGGAGTTATTGTCa > 1:277313/1‑149 (MQ=255) ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg > 1:587180/1‑149 (MQ=255) cAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCATAGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGCTCAAGTTGTAGGTAGTGATTGTCATgg > 2:478860/1‑149 (MQ=255) aGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGAGTCGCCAAGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGAAACGGTAGCACTGCGATCAGGTTTTGGGGAGTTATTGTCATAGc > 2:789048/1‑149 (MQ=255) cgcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAttt > 1:322925/1‑149 (MQ=255) gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCCACGCCAAGCCGATGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGGGTTGTTGTCATGGCGACTAACTGCCtt > 2:575138/1‑149 (MQ=255) cAATACCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta < 2:237669/149‑1 (MQ=255) cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCGGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta < 1:897245/149‑1 (MQ=255) tAACCGAGGGCGTAGCGTTGAGGAANTGATTGCGTCGCCAAGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTTTTGTCATGGCGATTTACGGCCTTgtagta < 1:614848/149‑1 (MQ=255) tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagta < 1:659492/149‑1 (MQ=255) tAACCGAGAGCGTAGCGTGGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagta < 1:462443/149‑1 (MQ=255) aaCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATGTGGGTGCTGGGAGTTATTGTCATGGCGATTTACGGCCTTgtagtat > 1:312777/1‑148 (MQ=255) gagaTCGTAGCGTTCAGGAAATGATTGCGTCGCCACGGAAGGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGAAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAAc < 2:454001/149‑1 (MQ=255) gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCCACGGTAGCAGTGCTATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAAc < 2:541822/149‑1 (MQ=255) tgcctagcgtTGAGCAAATCATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGa < 2:66484/145‑1 (MQ=255) gcgtagcgtTGAGGAAATGATAGTGGCACGATTCAGTCCACACCATC‑‑CAACTCCCCGCCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGaa < 1:125895/149‑1 (MQ=255) gcgtagcgtTGAGGAAATGAGTGCGGCGCCACGAAATGGCGACCATCGACAA‑‑TAGCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGaa < 2:440506/149‑1 (MQ=255) cgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACa < 1:518324/149‑1 (MQ=255) tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAAc < 1:122385/149‑1 (MQ=255) gAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt < 1:883493/90‑1 (MQ=255) gAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt > 2:883493/1‑90 (MQ=255) gAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCAct > 2:418989/1‑149 (MQ=255) caTTGCGTGGCGACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTgg > 1:666453/2‑149 (MQ=255) caTTGCGTGGCGACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTgg > 1:666408/2‑149 (MQ=255) gTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTg > 2:520009/1‑149 (MQ=255) gcGCCACGCAATGGAGCGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGc < 2:386777/148‑1 (MQ=255) | GCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATC‑ACAAC‑CACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGC > NC_000913_3_pae_tpiA/2391271‑2391561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |