| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 4,604,632 | C→A | A186E (GCA→GAA) | bglJ → | bgl operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,604,632 | 0 | C | A | 97.0% | 100.1 / ‑3.6 | 33 | coding (557/678 nt) | bglJ | bgl operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (16/16); minor base . (0/1); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGG > NC_000913_3_pae_tpiA/4604491‑4604768 | accgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGTCTACTCAATTACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATaaa > 1:325134/1‑149 (MQ=255) acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTCCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTa > 1:196622/1‑149 (MQ=255) ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGCTGTCGAAACTGAGc > 2:318175/1‑149 (MQ=255) ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGc < 2:134590/149‑1 (MQ=255) ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAAATGGGc > 2:253604/1‑149 (MQ=255) aCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCATTACGACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATAACAAAACGATCCGTAAACATAAATTTA‑ATGTGAAGTCGAAACTGGGCGTc > 1:560735/1‑149 (MQ=255) gCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCt < 1:613539/149‑1 (MQ=255) cGGAAGTTAATGCCGACGTAGAGAGAAATATTGTGTTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGa < 2:416208/149‑1 (MQ=255) ccgggcGGAGAGAGAAATATTGCGCTTGATGTCGGGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATATATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgt < 2:414078/144‑1 (MQ=255) agaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAACCGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGGGTCAGTTTTAACACAGGGTTGTTGGAGGCCGCAg > 1:595819/1‑149 (MQ=255) ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGc > 2:575706/1‑108 (MQ=255) ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGc < 1:575706/108‑1 (MQ=255) ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt > 2:665737/1‑149 (MQ=255) gTCGCGTGGCTACTCAATGACACGAATTGCCGAGCAGCTTAAGCGAAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAAGGTTGTTGGAGGCCGCAGATATTCTGTtatgtatga > 1:565485/1‑148 (MQ=255) gcgTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAGACGGAATCCATAGACTGTACGTGAATAGAGATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCAGCAGATTTTCTGTTATGTATGCgtc > 1:699324/1‑147 (MQ=255) cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGaaa < 2:500830/149‑1 (MQ=255) aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa > 1:435075/1‑149 (MQ=255) cacaAATTGCCGAGCAGCTTAACAGCAATTTCTTAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTGTGCGGCATTGCGAAACAAGTAAt > 2:156400/1‑149 (MQ=255) cacaAATTGCCGAGCAGCGTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt < 1:340819/149‑1 (MQ=255) acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg > 2:275011/1‑149 (MQ=255) acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTG‑AAACCAATAGATATTGCATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg < 1:318175/149‑1 (MQ=255) aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt < 1:665737/149‑1 (MQ=255) aTTGCCGAGCAGCTGACACGGCATATCAAATCGATACGTCAACTTAAATTAA‑ATGTTCGCTCGAAATATGGAGTCAGTCTTGACGCATGGTTGTGGGAAGCCGCAGATATGCTGTTATGTACGCGGCATTGCGAAACAAGTAATGTGtt < 2:699324/149‑1 (MQ=255) tgccagcTTAAACTTAATATCAAAACCATCCTTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc < 1:249471/146‑1 (MQ=255) gaagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCt < 2:390974/147‑1 (MQ=255) agcTTAAACGCAATATTAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTAt < 2:196622/149‑1 (MQ=255) cGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGTAACAAGTATGGTGTTGCATCCCTCTTAATCCGc > 1:181559/1‑149 (MQ=255) cGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATccc < 1:464814/138‑1 (MQ=255) cGCAATATCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATccc > 2:464814/1‑138 (MQ=255) cAATATCAAAACAGACCGGTAATATAAATTTA‑ATTTGATGTCGAAACTGGGCATCAGTTCTGACTCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat < 2:462310/149‑1 (MQ=255) tatCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg > 1:447893/1‑74 (MQ=255) tatCAAAACGATCCGTGAACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:447893/74‑1 (MQ=255) aaaCTATCCGTGAACTTAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGTGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCgg < 1:564241/149‑1 (MQ=255) | ACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTA‑ATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGG > NC_000913_3_pae_tpiA/4604491‑4604768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |