| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 4,295,976 | C→T | 14.8% | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,295,976 | 0 | C | T | 14.8% | 77.2 / 6.1 | 51 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (20/20); new base T (4/3); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC > NC_000913_3_pae_tpiA/4295828‑4296123 | gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAAc > 2:670923/1‑149 (MQ=17) gATAAGGCGTTTATTCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCt < 1:720787/149‑1 (MQ=17) tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGa < 1:169802/149‑1 (MQ=17) aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCAGCCGACATCAATGCCGGAtc > 2:479752/1‑148 (MQ=12) gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc > 2:467432/1‑149 (MQ=17) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt < 1:346339/149‑1 (MQ=17) tccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc < 1:304930/148‑1 (MQ=18) tcGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAAGGCCTGATGCGACCCTTGcc > 2:660130/2‑149 (MQ=21) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc > 2:40732/1‑149 (MQ=21) ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGATAAG‑GCGTTTACGACGCGTCCGAACTCAACGCCGGCTGCGACGCttcca > 2:58215/1‑146 (MQ=11) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAATCCCTGATGCTACCCGTGCcg > 2:285034/1‑149 (MQ=11) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg < 1:478046/149‑1 (MQ=35) cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg < 1:477924/149‑1 (MQ=35) gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCAGCCGCCGTCACGGCCTGCTGCGACGCTTGAcgc > 2:636595/1‑149 (MQ=11) cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg > 1:95720/1‑149 (MQ=38) gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCGAGACAGCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCCCATCCGACATCAACGCCTGATGAGGCGCTTGCCGCGTCTTa > 1:647074/1‑149 (MQ=11) cATCAACGCCTGATGCGACGCTTAACGCGTTTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc < 1:380869/149‑1 (MQ=255) cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc > 1:313419/1‑149 (MQ=38) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:735343/1‑149 (MQ=37) gCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAACAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa < 1:201179/149‑1 (MQ=255) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGATCCACCAC‑CCTCATGCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc > 1:168112/1‑149 (MQ=11) gACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGGTTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGAACTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 2:488882/149‑1 (MQ=11) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcaccccg > 2:163354/1‑143 (MQ=255) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCACCGCCTGCTGCGACGCTTGCCGCGGCTTATCCGGCACACGctcgact > 1:200049/1‑143 (MQ=11) aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa < 1:263450/149‑1 (MQ=39) aCGCGTCTTAGCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa < 1:126349/149‑1 (MQ=255) gaGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAG‑GCGTTTCCGCCGCATACGGCATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg < 2:658335/148‑1 (MQ=11) ggCCGACGGCAGAGAGAGCAAGGGTGTGATTGAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATTCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg < 2:587042/149‑1 (MQ=11) tcaCGTCAGACAGATGAATAGCTATATTTGGCGTTATTTTGTAGGGCGGATAAG‑GCGTTTACGCCGCATTCGACATCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa < 2:408498/147‑1 (MQ=11) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCTACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCATACAGAGCAATAGCCTGAATTAGTGTGAt > 1:6931/1‑149 (MQ=11) ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCACGCCTATCCCAGCCAGCGGAATAGCTTGATTTAGCGGCATTTTg > 2:366326/1‑149 (MQ=11) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg < 1:518032/149‑1 (MQ=38) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc < 1:379338/149‑1 (MQ=39) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:513663/92‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:513663/1‑92 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGCGTCTCGAATAAGGCg > 2:296518/1‑149 (MQ=11) ccTGATTTAGCGTGATTTTGTAGCTTGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTCATTTTGTAGGTCGGATACGGCg > 2:413524/1‑149 (MQ=14) tcgCGTGATTTTGTAGGTCGGATAAG‑GGGTTTACGGAGCTGCCCGCAGCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc < 1:503037/147‑1 (MQ=11) agtgTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc < 1:278451/146‑1 (MQ=255) gTGATTTTGTAGGTCGCATAAG‑GCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCATTAGCCTGATTTCGCGTGACTTTGTAGGTCGGATACGGCCTTTATGCCGCa > 1:641290/1‑149 (MQ=11) ggtcgggcGGACAAG‑GCGTTTACGCCCCACCCTACATCAACGCTTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa < 1:297377/142‑1 (MQ=11) tggggcGGATAGG‑GCGTTTAGGCCGCAGACGGCGTCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc < 2:233031/144‑1 (MQ=11) gatgTCGGATGCG‑GCGTAAACGCCTTATCCGACCTCAACGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTGTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc > 1:22408/4‑149 (MQ=11) gTCGGATAAG‑GCGTTTACTCCGCATCCGACATCAACGCCTGATGCGGCGCTTGCCGCATCTTATCAGGCCTACGCCAGACAGTGCAATAGCCTGATTATGCGTGATTTTCTAGGCGGGATCAGGCCATTCGGCCACATCCCtcatcaca > 2:699595/1‑147 (MQ=11) tCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGACATCCACg > 2:12001/1‑149 (MQ=255) gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc < 1:704299/149‑1 (MQ=38) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATGAGTGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg > 1:389109/1‑149 (MQ=255) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg > 1:709220/1‑149 (MQ=39) gcATCCGACATCAACGCCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc < 2:95720/149‑1 (MQ=21) acccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt < 1:360387/147‑1 (MQ=25) aaCGCCTGATGGGACGCTTGGCGGGGCTTATCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc < 2:440667/149‑1 (MQ=11) aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGTCATCAACGCCTGATGCGCCGTTTGACGCGTTTTGTCAAGca > 2:164550/1‑148 (MQ=11) | GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC > NC_000913_3_pae_tpiA/4295828‑4296123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |