| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 3,560,455 | 1 | . | G | 95.9% | 132.4 / ‑1.1 | 49 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (15/32); total (16/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGT > NC_000913_3_pae_tpiA/3560308‑3560594 | ccGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGcg < 1:557055/149‑2 (MQ=255) atCGATAAACAGCGTCGAGCCATTGGGGATTTGCTTCGTCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGa < 1:103097/149‑1 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTGCTTCGCTACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATc < 1:809969/149‑1 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATc > 2:772062/1‑149 (MQ=255) tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAgg < 1:951222/149‑1 (MQ=255) cAGCGTCGAGCCATTGGGCATTTGCTCCGCCACTTTGCGTGCGATGCGCTCTTTTTCTTCGGTCAGGGTGGCCTTGCGATCGTGCCACGGCTTGTTAACCGAACTGTAAGGGAGCGCCGCACCGCAGTGAGT‑GGC‑AAGAATCAGGtttt > 2:162951/1‑149 (MQ=255) aGCGTCGTGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTTTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTg < 1:200205/149‑1 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGGCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTcc < 1:277958/149‑1 (MQ=255) gtgcgaTTGGGGATTGGCTCGGCTACTTTGCGGCCGATGCGTTCTTTTTCTTGGGGCTGGGTGGCCTTGGGATCGGGCCACGGCGTGTTAACCGACCGGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTTCGc < 2:508744/144‑1 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACCGCGTGTTAACCGAAGTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCa > 2:789287/1‑149 (MQ=255) cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGc > 1:341851/1‑149 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 1:779074/149‑1 (MQ=255) aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 1:948930/149‑1 (MQ=255) cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc > 1:391760/1‑149 (MQ=255) ggCCACGTGGCGGGGTGTGCGCTCTTTTTGTTCGGTTTGGGTGGTCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 2:827544/148‑1 (MQ=255) cGCCACTGTGCGGGCGATGCGCTCTTTTTCTGCGGTGTGGGTGGGATGGCGATCGGTCCACCGCGCATTTAACAAACACGTATGTTGTTCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 2:521257/149‑1 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc > 1:824593/1‑149 (MQ=255) cccctcaTTTTCTGCCGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa < 2:200015/146‑1 (MQ=255) cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGTTTGTGTCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc < 1:193942/149‑1 (MQ=255) tcgtgggTCGGGGTGGCCTTGGGTTCGGGCTCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGA‑T‑GGCGCGGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGATCAGTCTGCGGGCTGACGGAGATATg < 2:86320/144‑1 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGAATCGTCTGCGGGCTGACGGCGAAATgtt > 2:607550/1‑147 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa < 2:763645/149‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc > 2:407006/1‑149 (MQ=255) ggcggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACAGCCATGA‑T‑GCCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGGTAGCGACGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAccgact > 2:841120/4‑145 (MQ=255) tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct > 2:875668/1‑149 (MQ=255) gcTTGCGATCGTGCCCCGGCGTGGTATCCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 2:678929/148‑1 (MQ=255) ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTGGCGGCGAATAGTCTGCGGTCTGACGGAGAAATGTTCTACCAGCTCTTCg > 2:194560/1‑149 (MQ=255) ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAGGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 1:331565/149‑1 (MQ=255) ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCATCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt < 1:330985/149‑1 (MQ=255) catgttccACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa < 2:213162/143‑1 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGTGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa < 1:378777/149‑1 (MQ=255) tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGTTCGCGTCGAATAATCTTCGGGCTGACAGAGACATCAACGACCACCGCTTAGGTACtaaa > 1:281162/1‑146 (MQ=255) ccgcctgtTAACCCAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTCCGGGCTGACGGAGAAATGCTCTATCAGCTCTTCGGTCCTGACATAACCct < 1:856136/144‑1 (MQ=255) tgtTAGCCGAACGTGAAGGCAGCGTCGCACCGCCATGA‑TGGGG‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGCCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 2:7630/149‑1 (MQ=255) gtTCACCTAACTGGAAGCCAGCTCCGCACCGCCAGGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTCACGTAGAAATGCTCTACCATCTCTTCGGTACTCATATTACCCTGCTGtt < 2:946944/149‑1 (MQ=255) gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 2:391760/149‑1 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa < 1:372625/149‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTTTTTAACCAGTTCg > 2:809509/1‑149 (MQ=255) ggAAGGCAGCGTAGCACGGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGGTCATTGAGGTCGCGGTGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGTTATTCGGTACTGACATAACCCTGCTGTTTAACCTGTTCGa < 2:734300/149‑1 (MQ=255) ggAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGc > 1:785279/1‑51 (MQ=255) ggAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGc < 2:785279/51‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATTCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 2:341851/149‑1 (MQ=255) aGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 1:178433/149‑1 (MQ=255) ggCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat > 1:289624/1‑149 (MQ=255) gCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata < 1:891479/149‑1 (MQ=255) aGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc < 1:599635/149‑1 (MQ=255) cgccgcACCGCCATGA‑T‑GGCGCAGGATCAGGTCTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt < 2:51438/149‑1 (MQ=255) ccATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTAGATAATACCGTTGTGACGTtg > 1:908696/1‑149 (MQ=255) tatga‑t‑gGCGCAGGATTAGGTTTTGCTCCGCCTGTTGATGGAGGGGGTTGTGAATGGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACTTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt < 2:477625/148‑1 (MQ=255) cATGA‑G‑GGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt < 2:979351/149‑1 (MQ=255) | CCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGT > NC_000913_3_pae_tpiA/3560308‑3560594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |