| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 4,296,297 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 1 | . | C | 92.5% | 92.6 / 2.6 | 42 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (18/19); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 2 | . | G | 92.7% | 98.6 / 2.7 | 42 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (19/19); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
AACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_pae_tpiA/4296200‑4296430 || aaCGCCTGATGCGACGCTTAACGAGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑A‑A‑‑CATC‑GTATCAGGCATATATGACTGGAGCGACGCCTCCCGAGTCTTATCTGGc > 1:139607/1‑149 (MQ=11) gCGACGCTTAGCGCGTCTTCTCAGGCCTACGCCAGACTGCCCATTAGTCTCATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc < 1:605935/149‑1 (MQ=1) cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt > 2:880457/1‑149 (MQ=255) aCGCGTTTTATCTGGCCTACGCCAGACATCGCAATTGCTTGATTTGGCGTGCATTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑TCATCTGACATCAATGCTTGATGCGTCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa < 2:53939/149‑1 (MQ=11) aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGttca > 2:412873/1‑147 (MQ=255) aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa > 1:597251/1‑149 (MQ=255) cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt < 1:603319/149‑1 (MQ=255) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc < 1:361027/149‑1 (MQ=255) aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGATCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCGTGCTGCGACGCTGTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCtaa > 2:112520/1‑149 (MQ=14) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCTCGTCTTATCATGCCTATCTTAACCGATAGTTAATTTTTCCAAc > 1:175693/1‑149 (MQ=14) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCacat > 1:386286/1‑146 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGTGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 1:787210/149‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 1:787223/149‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATATAGCGTGATTTTGTAGGTCGAATAGGGAGTT‑T‑ACGC‑‑C‑GCCGCCGACATCAATGCCTGCTGCGACGCTTGCCGCACCTTATCAGGCCTATCTTAACTGTGGGTTAATATTTCAAAGTCtt > 1:882580/1‑149 (MQ=14) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca < 1:340061/149‑1 (MQ=255) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑CG‑CGGCATCCGACATCAATGCCTGATAAGCCGCTTCCCGCGTCTTAACAGGCCTATCTTAACCGCTTGTTCATTTTTCATACTCTTca > 2:58704/1‑149 (MQ=14) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc > 2:223202/1‑149 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGAATCaa > 1:173798/1‑149 (MQ=255) cgcAATAGCCTGACTTCGCGTGATATAACTTGCCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCCGTCTCaa > 2:749201/1‑149 (MQ=17) gaaaTAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:209422/147‑1 (MQ=18) aatatgcTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCCTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc < 1:414028/143‑1 (MQ=18) aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc < 2:645119/149‑1 (MQ=255) aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTCTCCTAACTGTTGGTTACATCTTCAAACTCTTCACACCCGGTATCAAAcc > 1:923074/1‑149 (MQ=17) tAGCCTGATTCAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GGACCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt < 2:1019658/149‑1 (MQ=18) attcTGAGTTAGCGTGATTGTGTCGGTCGGATAAGGCGTC‑T‑ACGC‑‑C‑GCCTGCGCCATCAATGTCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACCGTGGGTTAATTTTTCAAACTCGTCACACCCGGTATCAAACCCtt < 2:561276/146‑1 (MQ=18) aGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCATT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt < 1:264369/149‑1 (MQ=21) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt < 1:813161/149‑1 (MQ=255) ggcTGATTTTCTGTGATGTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 2:462528/147‑1 (MQ=21) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACCCCCGGTATCAAACCCTTc > 2:295694/1‑149 (MQ=18) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ATGC‑‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc > 2:388800/1‑149 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 1:141720/149‑1 (MQ=255) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACACTTCCATACa > 2:388618/1‑149 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCCAACCCTTCCATACACCTCa > 2:504984/1‑149 (MQ=21) tGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCTGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg > 2:992316/1‑149 (MQ=255) gATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCATTACATACAGCTCAGGt > 2:135243/1‑149 (MQ=21) ttttGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc < 1:880457/149‑1 (MQ=255) tAGGTCGGATAAGGCGTT‑T‑ATGC‑‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa < 1:388800/149‑1 (MQ=255) ggTCGGATATGGCGTTGTCG‑‑C‑‑C‑GCTTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGt < 2:296222/149‑1 (MQ=37) gTCGGATAAGGCGTT‑A‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCAATACAGCTCCGGTTCAACCAGTg > 1:785318/1‑149 (MQ=25) tCGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACACTGGCCTTGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACATCTCAGGTTCAACCAGTGc > 1:464419/1‑149 (MQ=37) cGGATAAGGCGTT‑T‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGTGTTTTGCGGTGGCGATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa < 1:502016/149‑1 (MQ=25) taTAAGGCGTT‑T‑ACTC‑‑C‑TCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc < 1:489509/148‑1 (MQ=255) || AACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_pae_tpiA/4296200‑4296430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |