| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 1,544,022 | C→A | L13F (TTG→TTT) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 1,544,022 | 0 | C | A | 95.1% | 115.8 / ‑5.9 | 42 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (24/15); minor base T (2/0); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC > NC_000913_3_vch_tpiA/1543879‑1544161 | ttGAGATTAACGGTAACTGCGCTACATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTGTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑a‑aaaa < 1:337399/149‑2 (MQ=255) tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑a‑aaaag > 1:242931/1‑147 (MQ=255) gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTTTGGTTTCCAGTCTCG‑A‑a‑aaaaga > 2:577956/1‑146 (MQ=255) agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGCCTGCTATCCAGAGATTTCTTCGAGCAATATGATTTCCTTTATTTTGGCTGTAGCCCGGCTTTTCTGGTTTCCAGTCGCGAC‑A‑AAA‑GAt > 2:505904/1‑149 (MQ=255) gaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCTATTCGCG‑T‑a‑aaaagata > 2:815038/1‑144 (MQ=255) gaTTAACGGTAACTGCGCTAAATAGCATCAAGACACAGAAGGCAGGAAGTAGACGACAGACAGATATCCAAAGATTTCTTCGAGCACTATGTTTTCCTTTATTGTCCAAGAAGGCCGGATTTTCTCGTTTCCAGTCGCG‑A‑A‑AAA‑ggata > 1:703587/1‑145 (MQ=255) ttAACGGTAACTGCGCTAATTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGTAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAAc < 2:606348/149‑1 (MQ=255) ttAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATACCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAAc < 1:546495/149‑1 (MQ=255) tAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AAA‑GATAACg > 1:212362/1‑149 (MQ=255) gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTTCGGTTGTG‑T‑ATAAA‑GATAACGACTAttttttt > 1:361043/1‑147 (MQ=255) gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTtct > 1:362141/1‑149 (MQ=255) gcgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTAGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGAATATTTTtct > 1:572998/1‑149 (MQ=255) aGCATCCAGACACAGAAGGAAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCa < 1:226629/149‑1 (MQ=255) cATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCATTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGt < 1:121951/149‑1 (MQ=255) aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAAAAGGCCGGATTTTCTGGTTTCCAATCGCGAA‑A‑AAA‑GATAACGACTCTTTTTCTCATTTTGCatag > 2:21903/1‑146 (MQ=255) acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTg > 1:746485/1‑149 (MQ=255) aGAAGGCAAGAAGTAGACAACTGGCTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt > 1:331872/1‑149 (MQ=255) aGAACGCAAGAAGTAGACAACTGACTTATCACCAGAGCTGTCTTCGATCAATATGTTTTCCTTTATTTTCCCAGACGGCCGCTTTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATCACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt < 2:507421/149‑1 (MQ=255) tcgCAAAAAGTACACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGACGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt < 2:534670/147‑1 (MQ=255) agaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTATCATTTTGCAGTGCCATATTGTTCCTCACAt > 1:646326/1‑149 (MQ=255) aGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTATCATTTTGCAGTGCCATATTGTTCCTCACATGcaaac > 2:515730/1‑149 (MQ=255) aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg < 1:566520/149‑1 (MQ=255) aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg > 1:284324/1‑149 (MQ=255) tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTGTGGTTTCCAGTCGCG‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCCTATTGTTCCTCACATTCAAACATTGGTAATGa > 1:729594/1‑149 (MQ=255) tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTTTTCCTCACATGCACACATTGTTAATGa > 1:450815/1‑149 (MQ=255) cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg > 1:462564/1‑149 (MQ=255) agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATCCACACATTGTTAATGAAAAAAAGa > 2:278984/1‑149 (MQ=255) ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGCCTATTTTTCTCCTTTTGCAGTGCCATATTGTTCGTCCCATGCACTCTTTGGTAATGAAAAAAAGACACAACACg > 2:444463/1‑149 (MQ=255) tcGAGAAATATGTTTCCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 1:680287/149‑1 (MQ=255) gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATGGGTAATGACACAAAGACAAAACACGAgg > 1:68033/1‑149 (MQ=255) aaTATGTTTTCCTTTATTCTCCCAGAAGGCGGGATTTTCTAGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTCTTTTTCTCATTTTGCAGAGCCACATTGTTCCCCACATGCACCCATAGGTAATCAAAAAAAGACAAAACACGAGCTAAg > 2:580146/1‑149 (MQ=255) tatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTGTGGTTTCCAGTCGCG‑A‑A‑AAATGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCGCACATGCACACATTGGTAATGAAAAAAAACCAAAACACGAGGTAAGgc > 1:489221/1‑149 (MQ=255) tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa < 2:323833/138‑1 (MQ=255) tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa > 1:323833/1‑138 (MQ=255) ttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt < 1:624271/149‑1 (MQ=255) cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa > 2:207875/1‑114 (MQ=255) cAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa < 1:207875/114‑1 (MQ=255) gAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTTTTTTTTTTTAGTGCCATATTGTTCCTTACATGTACACATTGGTAATGAAAAAAAAACAAAATAGGTTGTACGTCGCAATAAGAAGTTATTAGAAtt > 2:324366/1‑149 (MQ=255) cggCCGGAGTTTCTCGATTCAAGACGAGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATGGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa < 2:451608/148‑1 (MQ=255) ggCGGGATTTTCGGGTTGCAAGTCGCGAA‑A‑AAA‑GATAACAACTATTTTTCTCATTTTGAAGTGCCATATTGTTCCTCACATGCAAACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg < 2:203282/149‑1 (MQ=255) ccGGATTTTCGGGTTTCCAGTCGCGAA‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa < 1:50586/149‑1 (MQ=255) cAGTCGCG‑AAA‑AAA‑GATAACGACTATTTTTTTTATTTTTCTTTGCCATATTGTTCCTCACATGAACACCTTGGTAATGAAAAAAAGACAAAACACGACGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATAAATTGAGTGAAATGc > 2:233588/1‑149 (MQ=255) | TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AAA‑GATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC > NC_000913_3_vch_tpiA/1543879‑1544161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |