| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 2,173,361 | 0 | C | . | 95.8% | 209.6 / 0.1 | 48 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base . (22/24); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_vch_tpiA | 2,173,362 | 0 | C | . | 95.8% | 205.7 / 0.9 | 48 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (22/24); minor base A (2/0); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAT > NC_000913_3_vch_tpiA/2173214‑2173501 || ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccacc < 2:675979/149‑1 (MQ=255) aTTTTCACGATGCACGGCGACCGTCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAATAGGCAGCACCTGATTACCCGGCACTCAATCAGCAACTAAAACGGTGACTGGGATAAGAATCAGGATTGCCGATaccaccgccgtat < 1:712831/149‑8 (MQ=255) aTTTCCACGATGGACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat < 1:270256/149‑8 (MQ=255) gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagc > 2:454639/1‑149 (MQ=255) atcgaCGGCGACCGCCATCGCCAAGAAGAAGCTGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca < 2:503042/145‑1 (MQ=255) gtgCGACCGGCATAGGCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc < 1:261481/147‑1 (MQ=255) cGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc < 1:831891/149‑1 (MQ=255) ccgccaccgccaCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑Ga < 1:244753/149‑1 (MQ=255) gCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATc > 1:599560/1‑149 (MQ=255) ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCa > 2:623010/1‑149 (MQ=255) cATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCaa < 1:590591/149‑1 (MQ=255) aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCGCCATCACATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAg < 1:112955/149‑1 (MQ=255) aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAg < 2:316938/149‑1 (MQ=255) aTCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGTCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAg < 1:302092/149‑1 (MQ=255) cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGc < 2:335740/149‑1 (MQ=255) cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCaa > 1:709522/1‑149 (MQ=255) gaagaaGCACATGGTGGCAAGATCGCCAAACCGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCa < 2:419267/149‑1 (MQ=255) gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCG‑GACCAAGGCCAATCAGGa > 1:152785/1‑149 (MQ=255) aagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGaa > 2:693205/1‑149 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCA‑GATCAAGGCCAATCAGGAGCTCCt > 2:408637/1‑149 (MQ=255) gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCGCATCAGGAATTCCTGACTGCCGAACtttg > 1:152024/1‑147 (MQ=255) gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCg > 1:719039/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc < 1:693205/149‑1 (MQ=255) gcagcaCGTGATGCCCGGGCCCTCAACCAGCAGTTAAAATGGCTGGTGGGCTAAAAGTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa < 2:106527/149‑1 (MQ=255) gcagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATTGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa < 2:106495/149‑1 (MQ=255) cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc < 1:454639/149‑1 (MQ=255) gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa > 2:624306/1‑149 (MQ=255) caCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAAGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGAc > 2:128951/1‑149 (MQ=255) tgaagaCCCGCCACACAAACAGCCAATAAAATGGGAATTGGGATAAAAATAAGGCTTACCGATACAA‑‑CCGCAGTATGGCCCAGCAGCAACGCCG‑GATCAAGGCAAATCAAGAACTACTGACCGCCCAGCTTCGCCTACAACCGACTAcc > 2:195511/6‑148 (MQ=255) gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa < 1:718093/149‑1 (MQ=255) gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa > 1:839087/1‑149 (MQ=255) tACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc > 1:350970/1‑149 (MQ=255) gCACACAGATAGTAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCAACGTCGTCATC‑GGGCCGATGAGGAAATCCTGGTCGCCGAACTTCGCCTTTAAACGACTACGAGCCTGCTTa < 2:390875/149‑1 (MQ=255) acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa > 1:699366/1‑149 (MQ=255) acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa > 1:699250/1‑149 (MQ=255) aCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg < 1:461020/149‑1 (MQ=255) aCAGCAATTAAAAAGGAAAGTGGGGTAAAAATCAGGCTTGCCGATA‑‑ACACCGCCGTATGTCCCAGGAGCAAGGCCG‑GATCAAGGCCAATGAGGAACTCCTGACCGCCGAACTTCGCCTGTCAACGACTACGAGCCTGCTTAGCGGTggg > 2:522391/1‑149 (MQ=255) gaagaaTTGAAATGGTGAGTGGGATTAAATTTTTGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCG‑GATCAAGTCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTTTAAACGACTACGAGCCTGCTTAGCGATGGGc < 2:658460/145‑1 (MQ=255) gCAATCAAAATGGTGAGTGGGATAAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt < 1:99539/149‑1 (MQ=255) aaTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa > 1:127794/1‑149 (MQ=255) ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa > 2:151601/1‑149 (MQ=255) ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCAG‑GATCAAGGCCAATCAGGAAATCCTGACCGCCGAACATCGCCTGTAAAAGACTACGAGCCTGCTTAGCGAAGGGAGATaaa > 1:174584/1‑149 (MQ=255) tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGTTGGGCGTTACACCATCCATGATgg < 1:729722/149‑1 (MQ=255) tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa < 2:558002/136‑1 (MQ=255) tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa > 1:558002/1‑136 (MQ=255) ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt > 2:367825/1‑124 (MQ=255) ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt < 1:367825/124‑1 (MQ=255) tAAAAATCAGGCTTGGCAATA‑‑ACACCGCCGTATGTCCCAGCAGCAACGCCT‑GATCAAGAACAATCAGGGACTCCTGACCTCCGTACTTAGCCTGTAAACGACTACGAGCCTGCTTAGTGATGGGCGTTGAACCATCCATAATGGGttga > 1:691241/1‑147 (MQ=255) gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAAGCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg > 1:586387/1‑149 (MQ=255) tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa < 1:285550/149‑1 (MQ=255) gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt < 2:719039/149‑1 (MQ=255) || GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCG‑GATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAT > NC_000913_3_vch_tpiA/2173214‑2173501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |