Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	3,815,825	C→T	intergenic (‑57/+9)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	3,815,825	0	C	T	100.0%	183.3 / NA	63	intergenic (‑57/+9)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

CCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCC  >  NC_000913_3_vch_tpiA/3815686‑3815967                                                                                                                                             |                                                                                                                                               ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAAt                                                                                                                                       >  1:70961/1‑149 (MQ=255) ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCACCCCTCCTCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGCCGTCTTAAAAAt                                                                                                                                       >  1:93722/1‑149 (MQ=255) cagtaTTTCAGAGTAAACTCGCTAAACTTTAACAACTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAAt                                                                                                                                       <  2:480156/145‑1 (MQ=255)   gtgtttCAGCGTAAACTCGGCAAACTTTAACACCTGCTTGCTAAGGGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACCTTGCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCa                                                                                                                                     <  2:269920/146‑1 (MQ=255)              tAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAgcg                                                                                                                          <  2:870102/149‑1 (MQ=255)                   tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCgccgcc                                                                                                                     >  1:412594/1‑149 (MQ=255)                   tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCCGATATCGTTACATGCCTTCTCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAACTCAGTTTGCCCGCgccgcc                                                                                                                     >  1:465845/1‑149 (MQ=255)                         aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATGTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                                               >  2:1440/1‑149 (MQ=255)                            tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                 <  2:846772/144‑1 (MQ=255)                            tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                 >  1:846772/1‑144 (MQ=255)                            tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                                            >  2:750043/1‑149 (MQ=255)                              tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                          <  2:70961/149‑1 (MQ=255)                              acactcttgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCAAAAAAC‑AAAAAAAACGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                          <  2:1040177/142‑1 (MQ=255)                                  acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                                                                      >  1:1005184/1‑149 (MQ=255)                                    cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                    <  1:625850/149‑1 (MQ=255)                                        ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                                <  2:1005184/149‑1 (MQ=255)                                          gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                                                                                              <  1:638016/149‑1 (MQ=255)                                            ttactgcAAATTTAATAATCTTGCTCTCATATGGATTCATCCCTTCTCTCCTCATCTTACTATTCTACAAAC‑AAACAAAACGCTCCTCATCAGTCGTCTTAAAAATTACTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgc                                                                                            <  2:22934/144‑2 (MQ=255)                                             aaGCGCAAGTTGAATAAACTGGCGCTTATGTGGTTTAATGTTTTGTGTTGGGTTCTTAGTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGTGTCGCTACAATGgattcgat                                                                                           <  2:111104/149‑1 (MQ=255)                                               gcgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                                         >  1:619757/1‑149 (MQ=255)                                                cgcAAATTCAATAAGCTGGCGCTGATACGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTcc                                                                                        <  2:697610/149‑1 (MQ=255)                                                 gcAAATTCAATAAACTGGCGCTGATATGGTTTCTTACCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccc                                                                                       >  1:1032851/1‑149 (MQ=255)                                                   aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGCTTCGATCCCCCt                                                                                     >  1:663726/1‑149 (MQ=255)                                                     aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCg                                                                                   >  2:828915/1‑149 (MQ=255)                                                            aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCgaga                                                                            <  2:800232/149‑1 (MQ=255)                                                             aaCTGGCGCTGATATGGTTTCAGGTGTTCGCTGTTCATCTTACTTTTCAACAGACAAAAAAAAAGGCGACTTATCAGCCGTGTTAAATATCAGTTTGCCAGCGCCGTCTTCTGCGTCGCTACAATGGATTCGATAGCTCTGTGGCCaga                                                                            <  2:549074/149‑1 (MQ=255)                                                                  gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac                                                                      <  1:1440/149‑1 (MQ=255)                                                                   cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGATTCGATTCCCCTCGGGCCAGAGccacca                                                                     >  2:582873/1‑149 (MQ=255)                                                                    gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                                    <  2:93722/149‑1 (MQ=255)                                                                       gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGCCCAGAGCCAACAAgtt                                                                 <  2:1032851/149‑3 (MQ=255)                                                                         ttaggTTTCATGCCTCCGCCCCTCATCTTACTTTTCAACAGAA‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa                                                               <  2:138842/146‑1 (MQ=255)                                                                           tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGCCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGCGt                                                             >  1:568833/1‑149 (MQ=255)                                                                           gggTTTCATGCCTGCGCTCTACATCTTACTGTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                             <  2:213629/148‑1 (MQ=255)                                                                             gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                           >  1:45023/1‑149 (MQ=255)                                                                              tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCCTCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCACCAACATGattggc                                                          >  2:645004/1‑144 (MQ=255)                                                                              tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCCTCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGGCGCTACAATGGGTTCGATTCCCATCGGGCCAGAGCCAACAAGCTGAGTaac                                                          >  1:539330/1‑147 (MQ=255)                                                                              tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCCACAAGCTGAGTacc                                                          >  2:104505/1‑147 (MQ=255)                                                                                         cGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                               <  1:689794/149‑1 (MQ=255)                                                                                          gCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                                         <  2:442035/138‑1 (MQ=255)                                                                                          gCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                                         >  1:442035/1‑138 (MQ=255)                                                                                             cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                           <  1:940438/149‑1 (MQ=255)                                                                                              ctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                          >  2:939497/1‑149 (MQ=255)                                                                                               tcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGCTTCAATTCCCCTCGGGCCAGAGCCAACAAGATTAGTAGCTCTTCTTGCGTGAACgg                                         >  1:419386/1‑149 (MQ=255)                                                                                                  tCTTACTTTACTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                      <  1:784194/149‑1 (MQ=255)                                                                                                     tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                   >  1:881649/1‑149 (MQ=255)                                                                                                        ttttCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAAAAAGATGGGTAGGTCTTCATGGGTGAACGGCTCGCCTTCt                               >  1:973564/1‑149 (MQ=255)                                                                                                            cTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                            <  2:881649/149‑1 (MQ=255)                                                                                                              aCAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                                 >  2:1039342/1‑126 (MQ=255)                                                                                                              aCAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                                 <  1:1039342/126‑1 (MQ=255)                                                                                                              aCAGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCCCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt                          <  1:320629/149‑1 (MQ=255)                                                                                                                aGAC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTACTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCCTGGGTGAACAGCTCGCCTTCTGACGGcc                        >  2:387297/1‑149 (MQ=255)                                                                                                                  aC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                                           <  1:131368/64‑1 (MQ=255)                                                                                                                  aC‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                                           >  2:131368/1‑64 (MQ=255)                                                                                                                   c‑AAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCCTGGGTGAACGGCTCGCCTTCTGCCGTCCCCt                     >  1:61734/1‑149 (MQ=255)                                                                                                                       aaaaaaGGCTACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                  <  1:939497/149‑1 (MQ=255)                                                                                                                         aaaaGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACAGCTCGCCTTCTGCCGTCCCTTGccgt                >  2:786946/1‑146 (MQ=255)                                                                                                                              gCGACTCATCATTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAat           <  2:864665/149‑1 (MQ=255)                                                                                                                              gCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTCGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCact           >  2:678893/1‑147 (MQ=255)                                                                                                                                gACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga         <  1:750043/149‑1 (MQ=255)                                                                                                                                     atcaGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc    >  1:349297/1‑149 (MQ=255)                                                                                                                                     atcaGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCACCTCGGGCCAGAGCCAACAAGTTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGATgcgc    >  1:909663/1‑149 (MQ=255)                                                                                                                                      tcaGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc   >  1:621895/1‑149 (MQ=255)                                                                                                                                       caGTCGTCTTAAAAATCAGTGTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc  <  1:31472/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               CCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCC  >  NC_000913_3_vch_tpiA/3815686‑3815967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑