Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,296,381	+GC	intergenic (+587/+55)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,380	1	.	C	95.7%	280.2 / 1.6	93	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (41/47);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*	NC_000913	4,296,380	2	.	G	95.7%	281.8 / 1.8	93	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (42/46);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTT  >  NC_000913/4296144‑4296666                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                                                                   taatgtctttagcgcccATCGGACATCAACGCCTGCGGCGACTGTTACCGCTTCTTCGCAGACCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                                                                                    <  1:773132/285‑1 (MQ=11) aaaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                                                                                    <  2:723757/300‑1 (MQ=2) aaaGGCGATGACGACGAATCCGACATCAAGGACGGATGCGACGCGGGCGGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                                                                                    <  2:83794/300‑1 (MQ=11)  aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                                                                                     <  2:141058/299‑1 (MQ=2)       gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                                                                                                                                                                                              >  2:456544/1‑301 (MQ=12)                 cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                                                                                                                                                                                                                     >  2:484974/1‑300 (MQ=255)                   tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑CCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCCGGCCTATCTTTACCGTTGGTTAATTTTTCaaaa                                                                                                                                                                                                                  >  1:366256/1‑300 (MQ=255)                             aCGCCTGATGCGACGCGTGCCGCGTCTTATCAGGGCTACGCACGACAGCGCAATAGTCTGATTTAGCGTTATTGTGTAGGTCGGATAAGGCGTTTAGGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                                        <  2:733324/301‑1 (MQ=14)                             aCGCCAGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                                        <  2:536797/301‑1 (MQ=255)                              cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTCGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACCTCCATTCCTGATGCTACCCTTGCCCCGGCTTATCAAGCCTACCTTAACCGTTGGGTACGTTTTTAACCtttttcaacc                                                                                                                                                                                                        >  1:156568/1‑294 (MQ=11)                                      gccacggttgcctcgTCTTAGTAGGCTTAGGCACGACAGCGCAGTGGCCTGATTTAGCGTGGTTTTGTAGGTCGGAGAAGCCGTGTATGCCGCACCCGAAATCAACGCCTGATGCGACCCCTAACGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGATCTAGCGTGATCTTGCAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGAGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc                                                                                                                                                                                                 <  2:519469/286‑1 (MQ=14)                                        caCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                                             <  2:797731/300‑1 (MQ=255)                                         gaggtggcgggtgttgaTCAGGCCGACGCAAGACGGCGCAATAGCATGATTTATCGTGATTTGGTAGGTCGGAGAAGGCGTTTATGCCGCATGCGACAGCAACGCCTGATGCGGCGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                                             <  1:518430/284‑1 (MQ=14)                                         aCGCTTGCCGCTTCTTAGCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                                                                                                                                                            <  2:704273/301‑1 (MQ=255)                                                ccgcgGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAt                                                                                                                                                                                     <  2:366266/300‑1 (MQ=255)                                                  tggTCTTATCATGCATACGACAGACAGCGTAATTGCCTGACTTAGCGTCATTTTTTAGGGCGTATAATGCGTTGAGACCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGGAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  1:310879/299‑1 (MQ=17)                                                  ccgTCTTATCAGGACTACGACAGACAGAGCCATGGCCTGATTTAGCGGTATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:722098/300‑1 (MQ=17)                                                   tgTCGTAGCAGGCATACGCCAGACAGCGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:183630/299‑1 (MQ=255)                                                   cttCTTATCAGGCCAAGGCCAGACAGCGCAAGAGCCTGATTGAGCGGGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCGTCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:170046/298‑1 (MQ=17)                                                   cggcTTAGCAGGCTTAGCGCAGACAGGTCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTGATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  1:154633/297‑1 (MQ=17)                                                   cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:668524/300‑1 (MQ=255)                                                   cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:449129/300‑1 (MQ=255)                                                    tttgtttCAGGGCTACGCCATTCATCTCAAGAGCCTGTAGTAGCGTCATTTTGTAGGTCGTATAGGTCGTTTATGCCTCATCCGACATCAACGCCTGTTGCGACGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg                                                                                                                                                                                 <  1:124034/295‑1 (MQ=17)                                                    gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAg                                                                                                                                                                                 >  2:510492/1‑301 (MQ=255)                                                       tgttCAGGCATACGCCAGAACGGGCAATGGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑Tc                                                                                                                                                                             <  1:346208/298‑1 (MQ=255)                                                        tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCa                                                                                                                                                                            >  2:112068/1‑301 (MQ=255)                                                        tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC‑ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATAAAGC‑TCAg                                                                                                                                                                           >  2:629187/1‑301 (MQ=255)                                                                 aaagCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAAcc                                                                                                                                                                   <  2:521352/298‑1 (MQ=255)                                                                  aCGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc                                                                                                                                                                                                 <  2:276408/271‑1 (MQ=255)                                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCTAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑AAGCAGC‑ATCT‑GACATCAATGACTGATGCGAGGCTTGCCGCGTCGTATCAGGCATATCTTAAACGTTGGTTACTTTTTCCAACTCTGTCCACCCGTTATAAAACCCTTCCATACCGC‑TAAGGTTccacca                                                                                                                                                                  >  1:32695/1‑301 (MQ=14)                                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGC‑TCAGGTTCAACCa                                                                                                                                                                  >  2:719316/1‑301 (MQ=255)                                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGTTTCACACCCGGTATCAAACCCTTCCCTACCGC‑TCGGGTTCAACCa                                                                                                                                                                  >  2:218922/1‑301 (MQ=255)                                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCAACTCTTGCCTCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTTAACACTCTTCACACCCGGtctccggtcccttataccactttcacgcttcct                                                                                                                                                                    >  1:276398/1‑271 (MQ=14)                                                                            gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCTCGTCTTATCTGGCCTATCTTCACCGTTGTTTAATTATTCAAAATCTTCACACCCGGTATCAAACCCTTCCATACACC‑TCAGGTTCAACCAGTTCAgcgc                                                                                                                                                         >  2:647531/1‑300 (MQ=17)                                                                             cgcAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAgcgc                                                                                                                                                         <  2:45974/299‑1 (MQ=255)                                                                             agaatcagccggttttggatggATTTTGTAGTGCGGATAAGGCGTTGACGCCGCGTCCGACCTAAAGGCCGTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAgcgc                                                                                                                                                         <  1:28383/278‑1 (MQ=18)                                                                                 aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGAAGCGCCTTTTg                                                                                                                                                   <  2:506935/301‑1 (MQ=255)                                                                                    gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGTCCTATCTTATCCGTTGGTTTCTTTTTCAAACTCTTCACACCCGTTATCTAACCCTTCCATATCGC‑TCACGTTCCACCATTCTACACCCTTTTGCtt                                                                                                                                                >  1:228405/1‑301 (MQ=14)                                                                                                   aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCCGTGCAACGCCTTTTGCTTGTTCTTCTCGCTaaa                                                                                                                                 >  2:171710/1‑301 (MQ=255)                                                                                                              tgggcaaGGCGTTTATGCCCGATCCTCCATCAACGCCGGAGGCTACGCGTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                                                                      <  1:383722/296‑1 (MQ=21)                                                                                                                gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAAcc                                                                                                                                                                   >  1:317792/1‑254 (MQ=255)                                                                                                                gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAAcc                                                                                                                                                                   <  2:317802/254‑1 (MQ=255)                                                                                                                       cgtttgttccgcaggcgatctgacagCCTGATACGAGGCGTATCGCGTCCTGTGAGGCCTACGGCGAAGAGCGGAATTGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCACTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                                              <  1:550251/275‑1 (MQ=25)                                                                                                                       cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGa                                                                                                             <  2:132038/301‑1 (MQ=255)                                                                                                                        tttatttccGCAGCAGACACCAACGCATGAGGCGACTCTTACCGCGTATTATCAGGCCGACGCCAGAAAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGaa                                                                                                            <  1:856333/294‑1 (MQ=25)                                                                                                                         tttgtgccggtgacgaagtgaaCGCCTGAGGCGCGCCTTGACGCGTCTTATAAGGCGTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAc                                                                                                           <  1:56867/281‑1 (MQ=25)                                                                                                                         tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGCTGGTTAATTTTTCAAACTCTTCACACCCGTTATCCAACCCTTCCATCCAGCTTCAGTTTCAA‑CATTTCTCTCCCTTTTTCTTTTTCCTCCCGTTAAAGCTTTTCTCACCGTTTGAGaaa                                                                                                           >  1:551572/1‑300 (MQ=14)                                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                                                                                         <  2:872129/301‑1 (MQ=255)                                                                                                                            aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                                                                      >  2:280634/1‑287 (MQ=255)                                                                                                                            aTGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                                                                      <  1:280624/287‑1 (MQ=255)                                                                                                                                      cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttc                                                                                                                                            >  2:747767/1‑255 (MQ=255)                                                                                                                                         cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTCTCTTAACCGTTGGTTACTTTTTCAAACTCTTCACACACGGTATCAACCCCTTCCATACATC‑TCAGGTTCACCCAGTCCAGCCCCTTTTGCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTaccaccactgtcgcttctaca                                                                                            >  1:743225/1‑280 (MQ=18)                                                                                                                                         cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAca                                                                                                          <  2:743252/286‑1 (MQ=255)                                                                                                                                           acaagtgcTGATGCTACGATTAAGGAGTCGTGACAGGCCTGAGCCGAGCAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGACGAATAAGGCGTTTACA‑‑CCAC‑ATCC‑GACATCAATGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGATATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATGCCCGCCCAGT‑Ac                                                                                        <  2:696969/294‑1 (MQ=25)                                                                                                                                             acggtcttaggCGACGATTAGCGCGTCTTGTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTc                                                                                      <  1:898711/291‑1 (MQ=255)                                                                                                                                               cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTAAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAAATCATTCCCGCCCAGT‑CCTCgg                                                                                    >  1:687289/1‑301 (MQ=255)                                                                                                                                                cccgtgtGCGACGCTTAACGCGTATGCTTGGGCCTAGGCCAGGAAGCGCAATAGGCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGa                                                                                   <  1:600778/295‑1 (MQ=37)                                                                                                                                                 ctggATGGGACGCTTAACGCGTCGTATAAGGCCTACGCGAGAAAGCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGa                                                                                   <  2:230460/297‑1 (MQ=37)                                                                                                                                                 ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGa                                                                                   <  2:730877/300‑1 (MQ=255)                                                                                                                                                        agtgttatacgagacagtATCAGGCCTGCGCCAGACGGCGCAATAGGCTGATGTAGCGTGATTGTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCg                                                                            <  2:8006/283‑1 (MQ=37)                                                                                                                                                               tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTtctc                                                                                                                                       >  2:234634/1‑235 (MQ=255)                                                                                                                                                               tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTtctc                                                                                                                                       <  1:234623/235‑1 (MQ=255)                                                                                                                                                                    cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                                             >  2:828501/1‑177 (MQ=255)                                                                                                                                                                    cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                                             <  1:828470/177‑1 (MQ=255)                                                                                                                                                                         tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATACAGC‑CCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCCCCGTTTACGAACATCATTCCCCCCCAGT‑ACTCGGAATacacgccgtcccttaaacaccta                                                          >  1:8006/1‑279 (MQ=25)                                                                                                                                                                          ttaccgcatACGCCCTACTGCGCAATCGCCCGATTTCGTGTGCTTTTTTGGCGCGTATACGGCCTTT‑‑A‑‑CCCCGCTCCACACATCAATGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTTGTTAAGTTTTCAACCTCTTCACACCCTGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGc                                                         <  1:484959/293‑1 (MQ=255)                                                                                                                                                                              ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCg                                                     >  2:799427/1‑301 (MQ=255)                                                                                                                                                                              ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCg                                                     >  2:373351/1‑301 (MQ=255)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAGTCTTCACACCCGGTATCAAACACTACCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTGCCCGCTAAAACCTTCGTCACCGTTTAAGAACATCATCCCAGCCCAGG‑CCCAGGAATAACCGGTTCGGTAAATTGCAGAGCTGCGGTTgg                                                >  2:532046/1‑300 (MQ=25)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATCCAGC‑TCAGGTTCAACAAGGGCAGCGACTTTTGCTTGTTCTTCTAGCTACACGCCTGTTCTCATTTTAAGAACAGATTTCCCGACCAGTCACT‑GGATCCACCTGTTCGGGCATTTGCacacgttcgtttca                                                >  1:242399/1‑288 (MQ=18)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   >  1:299773/1‑172 (MQ=255)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                   <  2:299783/172‑1 (MQ=255)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTATGACCATCATTCCCGCCCAGT‑ACTCGGAATACCCGGTTCGGGAAATTGCAGAGCTgattttaa                                                >  2:639210/1‑294 (MQ=255)                                                                                                                                                                                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCACTCCCGCCCAGT‑ACTCGGCATAACCGTTTCGGGAAATTGCAGATCTGCGTTTGa                                                >  2:635051/1‑301 (MQ=255)                                                                                                                                                                                    cgcgAGACAACGCATTAGCCTGATTTAGCGTGAATTTGTAGGTCGGATAAGGCGTTT‑‑ACTCCGC‑ATCC‑TACATCAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGaa                                               <  1:351413/301‑1 (MQ=255)                                                                                                                                                                                        aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCCCGATAAAACCTTCTTCACTGTTTAAGAACATCATTCCCGCCCAGT‑ACCCGGAATAACCGGTTCGGGAATTTGCAGAGCTGCGTTTgcacta                                            >  2:794004/1‑295 (MQ=255)                                                                                                                                                                                             gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCACTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATCATACCTTCTTCCCCGTTTAAGAACATCTTTCCCGCTCAGT‑ACGCGGAATCACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAcaacgt                                      >  1:149875/1‑296 (MQ=37)                                                                                                                                                                                              cgcAATGGCCTGTTTTATCGTTATTTTTTAGGTCGGATAATGCGTTTCCG‑‑CCGC‑ATCT‑GACATCAAGGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGt                                      <  1:799397/300‑1 (MQ=255)                                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAc                                           <  2:269543/295‑1 (MQ=255)                                                                                                                                                                                               gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGttt                                    >  1:75756/1‑300 (MQ=255)                                                                                                                                                                                                     gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTAATAACATCATTCCCGCCCAGT‑CCTCGGAATAAcc                                                                             >  1:1552/1‑254 (MQ=255)                                                                                                                                                                                                         gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATACCCGGTGCGGACCATTGCAGAGCTGCGTTTGAAATACCCGGTTGCCTTTTCAtt                          >  1:601887/1‑300 (MQ=38)                                                                                                                                                                                                                    aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTaa               >  2:91947/1‑301 (MQ=255)                                                                                                                                                                                                                    aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTAGATAAAACCTTCTTTACCGTTTAATAACATAATGCCCGCCAAGT‑ACTCGGCATAACCGGTTCGGGAAATTGCACAGATGCTTTTGATATACCAGGTTGCCTTGTCATCGTCGCCCTTaa               >  2:713647/1‑301 (MQ=38)                                                                                                                                                                                                                    aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑CCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCTCCGTTTAAGAACATCATTCACGCCCAGT‑ACCAGGAATAACCGGTTCGGGAAATTGCAGAGCCTGGTTTGAACTACACGGTAGCCTTTGCAtcgtcacgcttaa               >  1:389760/1‑293 (MQ=37)                                                                                                                                                                                                                      tttGTAGGTCGGATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATg             <  2:563194/301‑1 (MQ=255)                                                                                                                                                                                                                      ttggTAGGTCGGATAAGGCGTTT‑‑ACGCCGA‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATg             <  2:156575/298‑1 (MQ=255)                                                                                                                                                                                                                              ccGGAGAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGcc     <  2:903896/300‑1 (MQ=255)                                                                                                                                                                                                                                ggATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑CCTCGGAATAACCGGTTCGGGAAATTGAAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCTTCGGCCTTAATGCCCACCCCGt   >  1:443741/1‑301 (MQ=255)                                                                                                                                                                                                                                 gATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCATTTTGCTTGTTCTTCTCGATAAACCCTTCTTACCCGTCTATGAATATCATTCCCGCCCAGT‑ACTCGGACTCACCGGTTCGAGACATCGCAGAGCTGCATTTGAAATACCAGGTTGCCTTGTCATCTTCTGCCTTCATCCCCAccctg    >  1:618826/1‑295 (MQ=38)                                                                                                                                                                                                                                 gATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGCATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATTCCCACGCCGtt  >  1:277168/1‑301 (MQ=255)                                                                                                                                                                                                                                 gATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGTTTGCCTTGTCATCTTCGGCCTTATTGCCCACGCCGtt  >  2:480376/1‑301 (MQ=255)                                                                                                                                                                                                                                 gATAAGGCGTTT‑‑ACGCCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATTCCCACGCCGtt  >  1:454925/1‑301 (MQ=255)                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                                                                   TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGC‑ATCC‑GACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGC‑TCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTT  >  NC_000913/4296144‑4296666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑