Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	42.9%	100.7 / 58.9	91	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (8/44);  new base T (39/0);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 3.84e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206286‑3206585                                                                                                                                                      |                                                                                                                                                        aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTcc                                                                                                                                                        <  2:678908/151‑1 (MQ=255)     tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCaca                                                                                                                                                     >  1:449913/1‑148 (MQ=255)      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                                   <  1:1170902/151‑1 (MQ=255)      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGGTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAc                                                                                                                                                    <  2:708306/150‑1 (MQ=255)        ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa                                                                                                                                                  <  2:688145/150‑1 (MQ=255)        ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa                                                                                                                                                  <  2:1354775/150‑1 (MQ=255)          cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAt                                                                                                                                                <  2:262404/150‑1 (MQ=255)                aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑aaaaa                                                                                                                                        <  2:98613/151‑1 (MQ=255)                 cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATAA‑AAAAAc                                                                                                                                       >  1:629192/1‑151 (MQ=255)                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAAAAAATAC‑aaaaaat                                                                                                                                      >  2:109599/1‑149 (MQ=255)                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAACt                                                                                                                                      >  1:600746/1‑151 (MQ=255)                    tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTg                                                                                                                                     <  1:1149345/150‑1 (MQ=255)                     gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTgg                                                                                                                                    <  2:820421/150‑1 (MQ=255)                      ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATgg                                                                                                                                    >  1:1021297/1‑149 (MQ=255)                               gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCatga                                                                                                                         <  1:1045240/151‑1 (MQ=255)                                ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAA‑AAAAAATGGAGGTTCCatgat                                                                                                                        >  1:792215/1‑151 (MQ=255)                                 tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCatgatg                                                                                                                       >  2:85800/1‑151 (MQ=255)                                   tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCctgatg                                                                                                                       >  2:840831/1‑149 (MQ=255)                                   tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAC‑AAAAAATTGAGTTGCCatgatg                                                                                                                       >  1:1417989/1‑149 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAg                                                                                                                     <  2:343282/150‑1 (MQ=255)                                       gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTAAAAAAAAC‑AAAAAATGGCGCTGTCATGATGAGTGa                                                                                                                  >  1:974511/1‑150 (MQ=255)                                        cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGAAATTTTCAGCTAAATAA‑AAAAAACTGAGTTGTCaaaattatcgtt                                                                                                                 >  1:1271224/1‑139 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAA‑AAAAAATGGAGTTGTCATGATGAGCgga                                                                                                                 >  1:972423/1‑147 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAAATGGAGGTTCCACGATGAGCGaaa                                                                                                                >  2:47792/1‑150 (MQ=255)                                            attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCATGATGAGGGAAAgaa                                                                                                             >  1:686284/1‑148 (MQ=255)                                            attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAATAC‑AAAAACTGGAGATGCCCTGATTAGCGAAAGta                                                                                                             >  2:1261918/1‑150 (MQ=255)                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCATGATGAGCGAAAGtaat                                                                                                           >  2:1344296/1‑150 (MQ=255)                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                           <  1:1143267/150‑1 (MQ=255)                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGtaata                                                                                                          <  2:188563/150‑1 (MQ=255)                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAAATGGAGTTGGCATGATGAGCGAAAGtaata                                                                                                          >  2:411503/1‑150 (MQ=255)                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAA‑AAAAAATGGGGTTGGCCTGATGAGCGGAAGtaaaa                                                                                                          >  1:98653/1‑148 (MQ=255)                                                 ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAA‑AAAAAATGGAGTTTTTATGAATAGCGGAAGgaataaac                                                                                                       >  1:893060/1‑149 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                      <  2:132741/148‑1 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                      <  1:1138078/148‑1 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                    <  2:129678/150‑1 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                    <  2:257995/150‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAAATAA‑AAAAACTGGAGTTGCCATGATTAGCGAAAGTAATAAacaaca                                                                                                   >  2:473185/1‑150 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                  <  2:1031049/151‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAC‑AAAAAATGGGGGTGCCCAGATGAGCGAAAGTAATAAAAAACa                                                                                                   >  1:81533/1‑150 (MQ=255)                                                         ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGATGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                <  2:548421/150‑1 (MQ=255)                                                           cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAA‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAgg                                                                                             >  2:271137/1‑150 (MQ=255)                                                            gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCTATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                            <  2:808271/151‑1 (MQ=255)                                                             cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                           <  2:443829/151‑1 (MQ=255)                                                             cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCTGATGAGCGGAAGTAATAAACAAAAGGCAGTg                                                                                            >  1:747438/1‑150 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                         <  1:1409510/150‑1 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                         <  2:122208/150‑1 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAA‑AAAAAATTGAGGTGGCCTGATGAGGGAAAGGAAAAAAAAAAAGGCAGTGAAt                                                                                         >  1:863097/1‑150 (MQ=255)                                                                 gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATACAAAAAACTGG‑GTTGCCATGATGAGCGAAAGTAATAAAAAAAAGGCAGGGaaaa                                                                                        >  2:869951/1‑148 (MQ=255)                                                                 gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                        <  1:102481/150‑1 (MQ=255)                                                                   ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAATGGAGGTGCCCTGATGAGCGAAAGTAATAAGCAACAAGCAGTGGATAAGt                                                                                     >  2:166949/1‑151 (MQ=255)                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAACAACAGGCAGTGAATAAGttt                                                                                   >  2:787362/1‑149 (MQ=255)                                                                       cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAAAAAAAAA‑AAAAAATGGAGGTGCCATGGTGAGGGGAAGTAATAAACAACAAGCAGTGAaaaaagtga                                                                                  >  1:618531/1‑145 (MQ=255)                                                                       cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc                                                                                 <  2:470526/151‑1 (MQ=255)                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                <  2:963098/151‑1 (MQ=255)                                                                            tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACCAATAC‑AAAAACTTGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                               >  2:1380710/1‑148 (MQ=255)                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                             <  1:1167578/149‑1 (MQ=255)                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                           <  2:690332/151‑1 (MQ=255)                                                                               tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACCAATAA‑AAAAACTGGAGGTGCCATGATGAGTGGAAGGAATAAACAAAAGGCAGTGAAAAAATTGACAgggaatt                                                                         >  2:1193644/1‑148 (MQ=255)                                                                               tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAAAAGGAGAGAAAAAGATGACAGAGATTg                                                                         >  2:156517/1‑151 (MQ=255)                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                         <  1:1237907/150‑1 (MQ=255)                                                                                 aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAAC‑AAAAAAAGGAGTTGGCATGAAGAGCGAAAATAATAAGCAACAAGAAGGGAATAAAGTGACAGAGATTGtt                                                                       >  1:1155434/1‑150 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                   <  1:443445/151‑1 (MQ=255)                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAATGTCGCTa                                                                   >  2:1008810/1‑150 (MQ=255)                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa                                                                  <  1:976353/151‑1 (MQ=255)                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGTTGCCATTATGAGCGAAAGTAATAAACAACAAGCAGTGAAAAAAATTACAGAGAATTTTGCCAACt                                                                >  2:55411/1‑151 (MQ=255)                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                             <  2:777848/151‑1 (MQ=255)                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCCGACAAATAT‑ACAAAACGGAGTTGGCACTGTGAGCGAAAAGAAAAAACAAAACGGAGGGCATTAGATGACAGCGATTGTTGCTCACtttt                                                             >  2:14127/1‑150 (MQ=255)                                                                                              ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                          <  2:789322/151‑1 (MQ=255)                                                                                               gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAAAC‑AAAAAATGGAGGTGGCATGATGAGCGAAAATAAAAAACAACAAGCAGAGAAAAAGTTGACAGAGATTGTCGCTAACTTTTCCGc                                                         >  1:737520/1‑151 (MQ=255)                                                                                                 ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGCCATGATGAGCGAAAGGAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                        >  2:1258818/1‑150 (MQ=255)                                                                                                  gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCAt                                                      >  2:766488/1‑151 (MQ=255)                                                                                                       aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAACGAAATAC‑AAAAACAGGGGGTGGGATTAGGCGGGAAAGAAATAAACAAAAGGGAGGGGAAAAGGTTATACAGAATTTCGTTTAATTTACCGCCAAGAttt                                                 >  1:1142545/1‑151 (MQ=255)                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtttt                                                >  2:660421/1‑150 (MQ=255)                                                                                                         atatAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAGGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCTCTAACTTTACCGCCATGATTTCt                                               <  2:220079/151‑1 (MQ=255)                                                                                                         atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAAATCGGGCTGCTCTGATCAGTGAAAGTAATAAGCAAAAATAAGCGAAAAAGATGACAACAAATTTTGATAACTTTACCGccctccatttt                                               >  1:434035/1‑142 (MQ=255)                                                                                                         aaaaaaaaCCACATTTTTAGGGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                               <  1:949523/147‑1 (MQ=255)                                                                                                           ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGCCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                             <  2:192662/151‑1 (MQ=255)                                                                                                           aaaaaaCCACATTTTAAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAA‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                             <  1:403834/149‑1 (MQ=255)                                                                                                            taAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCAGACAAATAA‑AAAAACTGGAGGTGCCATGATTAGCGAAAGAAATAAGCAAAAAGCAGTGAATAAGTTGAAAGAGATTTTCGCTAACTTTACCGCCACGATTTTTAcc                                            >  2:1183761/1‑151 (MQ=255)                                                                                                             aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                                           <  1:339383/151‑1 (MQ=255)                                                                                                               aaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                         <  2:1251381/151‑1 (MQ=255)                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                      <  1:840832/151‑1 (MQ=255)                                                                                                                   ccaTTTTTAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGCCAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGc                                     <  2:711120/150‑1 (MQ=255)                                                                                                                    caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGGGCGAAAGGAATAAACAAAAGGGAGTGAATAAGGTGACAGAGATTGTCGCAAACTTTAACGCCATGATTTTTATCCGAATGcc                                    >  2:911129/1‑151 (MQ=255)                                                                                                                       ttttAGTGGTATTTTGGGGGGTTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGACCAAAAGAAACAGGCAACAGGCAGTGAATAATTGGCCAAAGTTTTTCGCAACCTTTACCGCCATGATTTCTCCCCGAATGCCtg                                  <  2:656995/150‑1 (MQ=255)                                                                                                                           aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                             <  2:345589/151‑1 (MQ=255)                                                                                                                                 aGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTTGCATGATGAGGGGAAGTAATAAACAACAAGCAGTGGATAAATTGACAGAGATTTTTGCTAAATTTAACGCCATGATGTTTACCCGAATgacgaaggtggagggg                       >  1:1135944/1‑136 (MQ=255)                                                                                                                                    ttGTGGCGGTGAATTTTTTCCGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGGAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCGAAAGCCTGATGAAGTGGGGGAt                    >  1:452763/1‑151 (MQ=255)                                                                                                                                      gtgGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                  <  2:972427/151‑1 (MQ=255)                                                                                                                                      gtgGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                   <  2:147228/150‑1 (MQ=255)                                                                                                                                                 tttttCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTGCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc       >  1:1425987/1‑151 (MQ=255)                                                                                                                                                  ttttCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  1:434844/1‑151 (MQ=255)                                                                                                                                                      ccAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:332639/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206286‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑