Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	45.5%	124.6 / 24.9	88	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (18/30);  new base G (40/0);  total (58/30)
Fisher's exact test for biased strand distribution p-value = 4.61e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434740‑2435030                                                                                                                                               |                                                                                                                                                      atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                               <  1:152509/151‑1 (MQ=255) atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGAAGATCATACCGGGCAAGGTAAaccacc                                                                                                                                               >  2:1141672/1‑151 (MQ=255)  tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAGCATACCGGGCAGGGGAAaccaccc                                                                                                                                              >  2:1286615/1‑150 (MQ=255)   atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCAcccc                                                                                                                                             >  1:429053/1‑149 (MQ=255)     ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACcccg                                                                                                                                            >  2:611978/1‑150 (MQ=255)      tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAAAACATAACGGTCAGGGGAATTCACCACGAc                                                                                                                                          >  1:585772/1‑151 (MQ=255)        ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCa                                                                                                                                        <  2:454131/151‑1 (MQ=255)           aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGAcc                                                                                                                                         >  1:52818/1‑147 (MQ=255)           aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGTTCATACCGGTCGGGGTAATCCACCGCGACCAgg                                                                                                                                      <  2:516044/150‑2 (MQ=255)           aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGCGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAg                                                                                                                                       <  2:525251/149‑1 (MQ=255)                  gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGccc                                                                                                                              >  2:353208/1‑151 (MQ=255)                    aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt                                                                                                                            >  1:337811/1‑151 (MQ=255)                    aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCtt                                                                                                                            >  1:329870/1‑151 (MQ=255)                       cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                          <  1:874771/150‑1 (MQ=255)                        gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑c                                                                                                                       >  1:1254945/1‑151 (MQ=255)                        gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCACCGGCGGCTTTGGCAGATCATACCTGCCCGGGTAATCCACCGCGACCAGATATAGCCCTTTCG‑c                                                                                                                       >  1:1416236/1‑151 (MQ=255)                          cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑Ctt                                                                                                                     >  2:1448853/1‑151 (MQ=255)                              tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGc                                                                                                                 >  1:298131/1‑151 (MQ=255)                               ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCG‑CCTTTGcc                                                                                                                >  1:51477/1‑151 (MQ=255)                               ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTg                                                                                                                  <  2:39013/149‑1 (MQ=255)                               ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAATCCCCCGCGACCAGATACAGCCCCTCCC‑CTTTTGcc                                                                                                                >  2:1218624/1‑151 (MQ=255)                                tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATATCGGTCACGGGAATACACCGCGACCAAGGACAGCCCTTCCT‑Ctttttgcg                                                                                                               >  2:1363068/1‑147 (MQ=255)                                    gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTtgc                                                                                                           <  2:1295882/151‑1 (MQ=255)                                    gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTGCCGtttc                                                                                                           >  2:639477/1‑149 (MQ=255)                                       gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTtgctgc                                                                                                        >  1:379468/1‑151 (MQ=255)                                       gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTGTTTGCAAATCACACCGGTCCCGGGCATCCACCGCGAACCGATAAAGTCCTTTCC‑GTTTTGGCGttcatgc                                                                                                        >  2:390912/1‑146 (MQ=255)                                        tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgcc                                                                                                       >  2:294628/1‑151 (MQ=255)                                         aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgccc                                                                                                      >  1:592378/1‑150 (MQ=255)                                             aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCCGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTGCTgccccccg                                                                                                  >  2:25986/1‑149 (MQ=255)                                             aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTgccgcc                                                                                                    <  2:718369/149‑1 (MQ=255)                                             aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCa                                                                                                   <  1:1330383/150‑1 (MQ=255)                                                 tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGGCGTTGCTGCCGCCAGCGtt                                                                                              >  1:555703/1‑151 (MQ=255)                                                 tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAAACCACCGCGACCAGAGACACCCCTTTCC‑CTTTTGCCGGTGCTCCCCCCCGCGtt                                                                                              >  1:935449/1‑151 (MQ=255)                                                   gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCt                                                                                            <  1:180318/151‑1 (MQ=255)                                                     tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGGTGCTGCCGCCAGCGTTCTGt                                                                                          >  1:238997/1‑151 (MQ=255)                                                        aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCt                                                                                       >  1:631430/1‑151 (MQ=255)                                                              aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACCTACCGGTCCGGGTAAATCACCGCGACCAGAAACAACCCTTCCG‑CTTTTTCCGTTGCTGGCGCCCGCGCTCTGTCCTTTtccg                                                                                 >  2:824705/1‑151 (MQ=255)                                                                aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCAACGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTTCTGCCGCCCGCGGTccggtcggaagagcg                                                                               >  2:851852/1‑137 (MQ=255)                                                                aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTc                                                                                             <  1:851861/137‑1 (MQ=255)                                                                 tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGTAAACCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTtccgccc                                                                              >  2:959134/1‑150 (MQ=255)                                                                  gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATTAAACCGGCTAGGGGAATCCACCGCGACCCGAAAAAACCCTTCCG‑CCTTTGTCGTTCTTCCCGCCAACGGTCTGTCCTTTtccgcc                                                                               >  1:1247280/1‑149 (MQ=255)                                                                  gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccgcc                                                                               >  1:878575/1‑149 (MQ=255)                                                                     ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                           <  1:1302080/150‑1 (MQ=255)                                                                          cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                     <  2:1320097/151‑1 (MQ=255)                                                                              attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CCTTTTCCGGTGCTGCCGCCAGCGGTCTGGCCTTTGCCGCCCGCAACTCTGCTAt                                                                 >  2:24193/1‑151 (MQ=255)                                                                               ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTGAAGGTAATCCACCCCGACCAGAAACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTTCTATc                                                                >  2:853802/1‑151 (MQ=255)                                                                                tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAACCCTTCCG‑CTTTTGCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATcc                                                               >  1:281647/1‑151 (MQ=255)                                                                                  tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTTTCCTTTGCCGCCAGCAACTCTGCTATCCac                                                             >  1:1021696/1‑150 (MQ=255)                                                                                   cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                               <  2:441195/148‑1 (MQ=255)                                                                                     ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                          >  1:429474/1‑151 (MQ=255)                                                                                     ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCC‑CTTTTGCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGctc                                                          >  1:851973/1‑151 (MQ=255)                                                                                     ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                          >  1:316752/1‑151 (MQ=255)                                                                                        gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAGCATAACGGTCAGGGGAATACACCGCGGCCAGATACAGCCCCTCCG‑GTTTTGCCCGTGCTGGCCCCCGCGTTCTGGCCCTTGCCGCCAGCCACTCTGGTATCCACCTCTcc                                                       >  2:196287/1‑151 (MQ=255)                                                                                        gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                       <  1:145676/151‑1 (MQ=255)                                                                                        gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                       <  1:975520/151‑1 (MQ=255)                                                                                        gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                       >  1:704685/1‑151 (MQ=255)                                                                                          ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg                                                     <  2:1220757/151‑1 (MQ=255)                                                                                             ccAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGTCAAGGGAATTCATCCCGACCCGATAAAGCCCTTCCGACTTTTG‑CGTTTCTACCCCCGGCGGTCTGTCCTTTGCCGCCAGCAACTCTTCTATCCAGCCCTTCGGGTg                                                  >  2:1145130/1‑151 (MQ=255)                                                                                               aGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCCTCCG‑CTTTTTCCGCTGCTGCCGCCAGCGGTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTggg                                                >  1:434067/1‑150 (MQ=255)                                                                                                 aaaTAGCGGGCCCATTGGCGGTTTTGGGAGATCGTACCGGTCAGGGGAAACCACCGCCGCCAGATAAAGACCCTTCG‑CGTTTTCCGGTTGTGCCCCCACCGTTCTGTCCTTTGCCGCCAGCAACCCTGTTATCCAGCTCTTCCGCTgggtt                                              >  2:1379655/1‑148 (MQ=255)                                                                                                 aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAACGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTTCGGCTGGGtg                                              >  2:593518/1‑151 (MQ=255)                                                                                                   aTAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCG‑CTTTTTCCGTTGGTGCCGCCCGCGGTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGGtgtg                                            >  2:938764/1‑151 (MQ=255)                                                                                                   aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg                                            <  1:599567/151‑1 (MQ=255)                                                                                                   aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGtgtg                                            >  1:962286/1‑151 (MQ=255)                                                                                                     aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAAAGCCCTTCCG‑CTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCCGCTGGTTGTggg                                          >  1:901307/1‑151 (MQ=255)                                                                                                       cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa                                        <  1:742027/151‑1 (MQ=255)                                                                                                         ggCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCG‑CTTTTTCCGGTGCTGCCGCCAGCGGTCTGTCCCTTGCCGCCAGCAACTCTGCTATTCCGCTCTCCGGCTGGTTGTGGGCAcc                                      >  2:481455/1‑151 (MQ=255)                                                                                                         ggCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAAACCACCCCGACCAGATACAGCCCTTCCC‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCAcc                                      >  2:1108440/1‑151 (MQ=255)                                                                                                                tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                               >  1:1183078/1‑151 (MQ=255)                                                                                                                    ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                           <  2:1217896/151‑1 (MQ=255)                                                                                                                     gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                            >  2:378236/1‑149 (MQ=255)                                                                                                                       tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                         <  2:308687/150‑1 (MQ=255)                                                                                                                       tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        >  1:499614/1‑151 (MQ=255)                                                                                                                        ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        <  1:1228785/150‑1 (MQ=255)                                                                                                                        ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                       >  2:1170052/1‑151 (MQ=255)                                                                                                                           gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                    >  2:1357454/1‑151 (MQ=255)                                                                                                                               aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                <  1:387106/151‑1 (MQ=255)                                                                                                                                 cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat              <  1:1287349/151‑1 (MQ=255)                                                                                                                                 cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat              <  1:390906/151‑1 (MQ=255)                                                                                                                                    aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt           >  1:1273590/1‑151 (MQ=255)                                                                                                                                      cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         >  2:232443/1‑151 (MQ=255)                                                                                                                                      cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CCTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         >  1:56210/1‑151 (MQ=255)                                                                                                                                        gTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg       >  1:109593/1‑151 (MQ=255)                                                                                                                                          cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  2:591246/151‑1 (MQ=255)                                                                                                                                          cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  1:1218618/151‑1 (MQ=255)                                                                                                                                          cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     <  1:1099795/151‑1 (MQ=255)                                                                                                                                             ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  2:592377/151‑1 (MQ=255)                                                                                                                                             ggTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  1:611978/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                      ATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCG‑CTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434740‑2435030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑