Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,861	(T)8→7	intergenic (‑101/‑45)	dapA ← / → gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,854	0	T	.	100.0%	82.3 / NA	19	intergenic (‑94/‑52)	dapA/gcvR	dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/10);  total (9/10)

GAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGT  >  NC_000913/2599727‑2599998                                                                                                                                |                                                                                                                                                 gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGaa                                                                                                                           >  2:271086/1‑150 (MQ=255)             cgACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCtt                                                                                                              >  1:43486/1‑151 (MQ=255)                  aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                         <  2:143103/151‑1 (MQ=255)                        cccGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATaa                                                                                                    >  1:147719/1‑150 (MQ=255)                             gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                              >  2:262757/1‑151 (MQ=255)                                                     aCAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATc                                                                      <  1:198123/151‑1 (MQ=255)                                                                    gTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGt                                                       >  1:280417/1‑151 (MQ=255)                                                                        gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                   >  2:45582/1‑151 (MQ=255)                                                                         tttGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                   <  1:141414/150‑1 (MQ=255)                                                                               aTGGCATTAAAAGCAATCAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCTCCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGAt                                            <  2:146426/151‑1 (MQ=255)                                                                                         aagcaagcAGACAGAACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGaa                                  <  2:272062/151‑1 (MQ=255)                                                                                                cagacagaACCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGa                            <  1:267386/150‑1 (MQ=255)                                                                                                       aaCCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAacac                        <  1:89297/147‑1 (MQ=255)                                                                                                       aaCCGTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAac                          <  1:139297/145‑1 (MQ=255)                                                                                                           gTTCTGATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAcc                 >  1:38777/1‑150 (MQ=255)                                                                                                                gATTGTTGTATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCAt           >  1:52655/1‑151 (MQ=255)                                                                                                                   tgttgtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTc        <  1:168760/151‑1 (MQ=255)                                                                                                                       gtATGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagta    >  1:253666/1‑151 (MQ=255)                                                                                                                         aTGCATG‑TTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtagt  <  2:252606/151‑1 (MQ=255)                                                                                                                                |                                                                                                                                                 GAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGT  >  NC_000913/2599727‑2599998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑