| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,578,068 | 0 | T | C | 53.8% | 9.6 / 14.2 | 13 | I104V (ATT→GTT) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (0/7); total (2/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
CGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAA > NC_000913/2577926‑2578215 | cgcAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCg < 2:203867/150‑1 (MQ=255) aTGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGtt < 2:194391/151‑1 (MQ=255) aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑c < 1:16686/150‑1 (MQ=255) cAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑CACCGCCGTACCGTTaga > 2:108656/1‑151 (MQ=255) aaaaaaTTTGTCCGGGTCGATTTCGTCAACTTCAATGTCAAATCCATCAACCCCGGCGAATTTCTAAAACAGACCGCCCTTGCCTTCCATCCCCGGTTTGCCGGCCGGCGCGCCAATGTTGCCTACCCCCAG‑CACCGCCGTACCGTTagag < 2:276482/148‑1 (MQ=255) aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAACCCCGGCGAATTTCTAAAACAGAACGCCCTTGCCTCCCACCACCGGTTTGCCGGCCAGCGCGCAAATGTTGCCTACCCCCAG‑CACCGCCGTACCGTTagag < 2:99780/151‑1 (MQ=255) aTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTAAAATACATCAACCCCGGCGAATTTCTAAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTACCCCCAG‑CACCGCCGTACCGTTAgaga < 1:204125/151‑1 (MQ=255) ttaaaaTACATCAACCCCGGGGAATTTCTAAAACAGAACGCCCTTTCCTTCCATCCCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 1:224107/148‑1 (MQ=255) ataaaatccacaaaTCCCGGCGAATTTCTAAAAAAAAACCCCCTTCCCTCCAATCCCGGGTTTGCCTGCGCGCGCGCCAAT‑TTGCCCAACCCCCGAAACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 1:106577/139‑1 (MQ=255) taaaaTACATCAACCCCGGCGAATTTCTTAAACAGACCGCCCTTCCCTTCCATCCCCGTTTTGCCTGCCAGCGCGCCAATGTTGCTTACCCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 1:149473/148‑1 (MQ=255) taaaaTACACCACCCCGGGCAATTTTCTAAAAAAGACCCCCTTTCCCTCCCACCCCCGTTTTGCCTGCCAGCGCCCCAATGTTCCCTACCCCCAG‑CGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 2:186701/148‑1 (MQ=255) tCAAATCCATCAACCCCGGCGAATTTCTAAAACAGACCGCCCTTGCCTCCCACCCCGGTTTTGCCGGCCGGCGCCCCAATGTGCCCTACCCCCAG‑CCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 2:269837/150‑1 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa > 1:151929/1‑150 (MQ=255) | CGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAG‑CACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAA > NC_000913/2577926‑2578215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |