Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,599,4080AC61.1% 12.2 / 17.2 18V84G (GTG→GGG) livJbranched‑chain amino acid ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base C (0/11);  total (5/13)
Fisher's exact test for biased strand distribution p-value = 2.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-02

TCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATGAAGAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTACTGCGCAA  >  NC_000913/3599301‑3599546
                                                                                                             |                                                                                                                                          
tCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATGAAGAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTa                                                                                                  >  2:200144/1‑150 (MQ=255)
                   ttcgtAGATGTCAGACGCAGGCTGCGTTGATGAAGAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGc                                                                                >  2:144944/1‑149 (MQ=255)
                          aTGTCAGACGCAGGCTGCGTTGATGAAAAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCCCCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATgc                                                                       <  2:287439/151‑1 (MQ=255)
                                       gCTGCGTTGATGAAGAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCCCCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGt                                                           <  2:286650/150‑1 (MQ=255)
                                                   aagaaCAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAAc                                              <  1:277700/151‑1 (MQ=255)
                                                                    aTCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCgg                             >  1:270168/1‑151 (MQ=255)
                                                                         attttaaaT‑CCCTTCTTTAACGATTTTGTT‑CCCCCCCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAc                        <  1:308871/145‑1 (MQ=255)
                                                                          attttAATGGCCGTTTTTACCGA‑TTTGTTCCCCCCCCGAACCCGCCTGTTTCGGGTCACAGGCATCTTCATATTTTACGATTTGCAGTTTGTTGCTTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAAc                        <  2:121530/149‑1 (MQ=255)
                                                                           atTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACt                       >  2:262072/1‑150 (MQ=255)
                                                                                 t‑GCCGTCTTAAACGTTTTTTTT‑CCCCCCCCCAACCGCCTTTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTc                <  2:305657/151‑1 (MQ=255)
                                                                                   gcggtcgtaaccgtttttGTC‑CCCCCCCGCACCCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTc                <  2:259704/135‑1 (MQ=255)
                                                                                   gCCGTCTTTAACGATTTTTTT‑CGCCCCCGCACCCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTc                <  1:221636/150‑1 (MQ=255)
                                                                                         tttAGCGTCTTGGTC‑CCCCACCCCATCCGCCTGTTTCGGGTCACAGTCTTCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTAc         <  1:229805/150‑1 (MQ=255)
                                                                                         tttAACGCTTTTGTC‑CCCCCCCGCACCCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTAc         <  2:75449/150‑1 (MQ=255)
                                                                                         ttaaccgcttttGTC‑CCCCCCCCCACCCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTAc         <  2:14266/142‑1 (MQ=255)
                                                                                         ttaaacgcttttGTT‑CCCCCCCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTAc         <  1:127771/142‑1 (MQ=255)
                                                                                              cGATTTTTTT‑CCCCCCCGCACCCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTACTgcgc    <  1:36495/151‑1 (MQ=255)
                                                                                                aCTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTACTGCGCaa  >  1:236849/1‑151 (MQ=255)
                                                                                                             |                                                                                                                                          
TCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATGAAGAACAGAGGTGACCAATCACATATTTAAT‑GCCGTCGTTAACGACTTTGTT‑CGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGGTAAACTCCTGGTCACCGTACTGCGCAA  >  NC_000913/3599301‑3599546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑