| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,578,287 | 0 | T | C | 54.4% | 37.0 / 57.8 | 47 | E147G (GAA→GGA) | gntR | d‑gluconate inducible gluconate regulon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (14/7); new base C (0/25); total (14/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-08 | |||||||||||
TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAG > NC_000913/3578141‑3578427 | tgatAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTcca > 2:173207/1‑150 (MQ=255) atAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTccacca < 2:492482/151‑1 (MQ=255) tACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACggg < 1:432189/151‑1 (MQ=255) tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATAc < 1:390250/151‑1 (MQ=255) tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATAc < 2:107534/151‑1 (MQ=255) gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACtt > 2:455655/1‑150 (MQ=255) cgcCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAat > 2:252392/1‑151 (MQ=255) gagaTAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAatcat > 1:632371/1‑150 (MQ=255) aTAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCttt > 1:479335/1‑151 (MQ=255) caagggggCGACGCCCGCGAGAAATAAGGGCAGTGGTCATCTGGCGTGCTGCTTAAAAGTAACAAAAACC‑GACGGCAATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTCCCCCCACGGGAATACCCGCCACTTCAATCATCTTTAAgg < 1:190024/144‑1 (MQ=255) cAATGTGGCGATGCCCGCGAGCAATAAGGGCGGTGGTCATCGGGCGTGCTGCTTAAAATTTATAAAAACC‑GCCGGCGATATCAAGGCATGGCGACTG‑GCTGTCCATCAGTCCCACCACGGAAATACCCGCCACTTCAATCATCTTTAAgg < 1:12664/150‑1 (MQ=255) cAATGGGGCGATGCCCGCGAGAAATAAGGGAAGTGGTCATCTGGCGTGCTGCTTAAAATTTATAAAACCC‑GACGGCGATATCAAGGCAGGGCGACTT‑GCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg < 1:27183/150‑1 (MQ=255) aattgtgGCGACGCCCGCGAAAAATAAGGGCAGTGGTCATCGGGCGTGCTGTTTAAAAGTTATCAAACCC‑GCCGGCGATATAAAGGCAGGGCGTCTT‑GCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg < 2:357139/147‑1 (MQ=255) aatgggggggATGCCCGGGAGAAAAAAGGGCGGGGGTCATCTGGCGTGCTGCTAAAAATTTAAAAAACCC‑GACGGCGATATCAAGGCAGGGCGTCTT‑GCTGTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAgg < 2:62102/141‑1 (MQ=255) aaaTGTGGCGAGGCCCGCGAGCAATAATGGCGGTGGTCATCGGGGGTGCTGCTTAAAAGTTACAAAACCC‑GCCGGCGATATAAAGGCAGGGCGCCTT‑GCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg < 1:428389/149‑1 (MQ=255) aaTGGGGCGACGCCCGCGAGAAATAATGGCAGTGGTCTTCTGGCGTGCTGCTTCAAATTTACCAAAACC‑GACGGCAATATAAAGGCATGGCGACTT‑GCTTTCCATCAGTCCCCCCCGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTg < 2:394185/151‑1 (MQ=255) aTGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTg > 1:588891/1‑150 (MQ=255) cgAGAAATAATGGCGGTGCTCATCTGGCGCGTTTTTACAAAGTCA‑CAAAACCAGACGCCGATAGCAAGG‑GTGGGGGCTTCGCTTTCTTGCGTTGCCGCCGGGGAAAAACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTa < 1:269352/150‑1 (MQ=255) cAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt > 2:311843/1‑150 (MQ=255) cAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGCGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt > 1:465563/1‑150 (MQ=255) aaaaaatGGAGGTGTTCATCGGGCGTGCTCCTTAAAATTTATAAAACCC‑GCCGGCGATATCAGGGCAGGGCGATTT‑GCTGCCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 1:37704/147‑1 (MQ=255) taatGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGt > 1:377511/1‑151 (MQ=255) cggTGGTCATCTGGCGTGCTGCTTAAAATTTAACAAACCC‑GAGGGCGATATCAAGGCATGGCGACTT‑GCTGCCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGa < 1:84159/148‑1 (MQ=255) aaGTGGTCTTCGGGCGTGCTGCTTCAAATTTATAAAACCC‑GCCGGCAATATAAAGGCTTGGCGTCTT‑GCTGTCCATCATTCCCCCCAGGGAAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGa < 1:119031/149‑1 (MQ=255) ttattcgggcggcctgctTCAAATTTAAAAAACCC‑GCCGGCGATACAAGGGCAGGGCGATTT‑GCTGTCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGc < 2:259673/138‑1 (MQ=255) tCATCGGGCGTCCTCTTAAAAAGTTACCAAACCC‑GCCGGCAATAACAAGCCATGGCGTCTT‑GCTCCCCATCATTCCCCCCCCGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc < 1:637708/151‑1 (MQ=255) cATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg > 1:39222/1‑151 (MQ=255) gggTGCTGCTTAAAAGTTATCAAACCC‑GACGGCGATATCAAGGCAGGGCGACTT‑GCTGTCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:538113/148‑1 (MQ=255) ctgttaaaaaTTTATCAACCCC‑GCGGGGAATATCAGGGAGTGGCGTTTT‑TTTGCCCATCAGTCCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc < 2:347213/143‑1 (MQ=255) gctTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg > 1:269301/1‑151 (MQ=255) cttaaaattaaaaaaacCC‑GACGGCAATATCAAGGCATGGCGCTTT‑GCTTTCCATCAGTTCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg < 2:422272/137‑1 (MQ=255) ctTCAAATTTATCAAAACC‑GACGGCTATATCAAGGCATGGCGCCTT‑GCTGCCCATCATTCCCACCACGGGAATCCCCGCCACTTCAGTCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg < 1:216630/150‑1 (MQ=255) ttaaaattaaacAAACCC‑GCCGGCGATATAAAGGCATGGCGACTT‑CCTGCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 2:335659/140‑1 (MQ=255) ttaaaaGTTATAAAACCC‑GACGGCGATATCAAGGCAGGGCGACTT‑GCTGTCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 1:152877/148‑1 (MQ=255) tccAAAGTAACCAAAACC‑GACGAAAATATAAAGGAATGCCACGTT‑GCTGTCCTTCTGTCCCGCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 1:71417/149‑1 (MQ=255) tacAAAGTTAACAAAACC‑GACGGCGATACCAAGCCATGGCGCCTT‑GCTGTCCATCTGTCCCCCCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 2:43970/149‑1 (MQ=255) taaaaatttaaaaaacCC‑GCCGGCGATATCAAGGCAGGGCGTCTT‑GCTGTCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 2:611118/139‑1 (MQ=255) ccc‑GACGGCGATATCAAGGCATGGCGCCTT‑GCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAgg < 1:173210/150‑1 (MQ=255) tatCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt > 1:402241/1‑150 (MQ=255) tatCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt > 1:130840/1‑150 (MQ=255) tatCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt > 2:425368/1‑150 (MQ=255) cgtttt‑GCTGTCCATCATTTCCCCCACGGGAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGttt < 1:74510/146‑1 (MQ=255) ctattggCTGCCCATCAGTTCCACCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGttt < 1:162935/144‑1 (MQ=255) tt‑GCTGTCCATCATTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 1:153930/151‑1 (MQ=255) tggcgctCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 1:154311/145‑1 (MQ=255) cTGCCCATCATTCCCCCCAGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCg < 1:58036/150‑1 (MQ=255) tCCATCGGTCCCACCACGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAg < 1:538100/150‑1 (MQ=255) | TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACC‑GACGGCGATATCAAGGCATGGCGACTT‑GCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAG > NC_000913/3578141‑3578427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |