Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,862	0	G	A	71.4%	29.0 / 26.9	42	intergenic (+31/‑72)	yfbL/yfbM	putative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (3/9);  new base A (30/0);  total (33/9)
Fisher's exact test for biased strand distribution p-value = 4.93e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-06

GTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAA  >  NC_000913/2386725‑2387004                                                                                                                                          |                                                                                                                                                 gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACatt                                                                                                                                     >  1:487979/1‑151 (MQ=255) gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACatt                                                                                                                                     >  2:469855/1‑151 (MQ=255)      aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAAAAAAAAAGACAAGCACaatttttt                                                                                                                                >  1:581969/1‑151 (MQ=255)      aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACattttttt                                                                                                                                >  1:433690/1‑151 (MQ=255)         cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACatttttttttt                                                                                                                             >  1:610492/1‑150 (MQ=255)                         gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACACGCACATTTTTTTTTTTCTCCTCTCtttttaa                                                                                                             >  1:438616/1‑148 (MQ=255)                         gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTCGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGGCATGCACAATTTTTTTTTCCTCATCTGtttttaa                                                                                                             >  1:215570/1‑148 (MQ=255)                         gTGATACCGCAGACAGATTGAATTACCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAGACAAGCACATTTTTTTTTTTCTCATCTGtttttaa                                                                                                             >  2:239981/1‑148 (MQ=255)                          tGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGa                                                                                                            <  1:257416/151‑1 (MQ=255)                              aCCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGAAGC‑g                                                                                                       >  1:581704/1‑151 (MQ=255)                               ccGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTAAGATGC‑G‑a                                                                                                     >  1:332275/1‑151 (MQ=255)                                cGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑aa                                                                                                    <  1:547545/151‑1 (MQ=255)                                      cagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAAAAGC‑G‑GAAAATGa                                                                                              >  1:335721/1‑151 (MQ=255)                                        gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAAATGC‑G‑AAACATGaaa                                                                                            >  1:386996/1‑151 (MQ=255)                                         aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAAt                                                                                           <  1:198689/151‑1 (MQ=255)                                                       aaaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGC‑G‑GAAAAATAAAATCAAAGGGGGaaaa                                                                             >  1:78632/1‑151 (MQ=255)                                                               cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTATTTTTCCTCCTCTGTTTTTAGATGC‑G‑AAAAATGAAATTTAACGGAGGAAAAAAAAATgg                                                                     >  1:339229/1‑151 (MQ=255)                                                                 ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAGACAAGCACATTTTATTTTTTCTCCTCTGTTTTTAGATGT‑G‑AAAAATGAAAATCAACGGAGGAAAAAAAAATgggg                                                                   >  2:213815/1‑150 (MQ=255)                                                                     gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAGAAAAAACGACAAGAACAATTTTTTTTTCCTCATCTGTTTTTAAAAGC‑GAAAAAATAAAATTAAAGGGGGGAAAAAAAAATGGGatga                                                                >  2:162641/1‑148 (MQ=255)                                                                     gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAGGACATGGACAATTTTTTTTTCCTTATCTTTTTTTAAAAGC‑G‑GAAAATGAAAATTAAAGGGGGGAAAAAAAATGGGGATgg                                                               >  1:315394/1‑150 (MQ=255)                                                                       ggATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAAAGACAAGCACATTTTATTTTTCCTCCTCTGTTTTAAAAGAC‑G‑AGAAAAGAAAATTAACGGGGGGAAAATAAATGGGGATGAtt                                                             >  2:45172/1‑151 (MQ=255)                                                                            gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAAAGACAAGAAAATTTTATTTTTTCTCCTCTGTTTTTAGATGA‑G‑AAACAAGAAATTTAAAGGAGGGAAAATAAAATGGAATGAtttggta                                                        >  2:88330/1‑146 (MQ=255)                                                                            gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGC‑G‑AAAAATGAAATTCAAAGGGGGGAAAATAAATGGGGATGATTGGCTa                                                        >  1:72369/1‑151 (MQ=255)                                                                            gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAATGCACAATTTTTTTTTCCTCCTCTTTTTTAAGAAGCGG‑AAAAAGAAAATTCAA‑GGGGGGAAAATAAATTGGGATGGTTgggtt                                                        >  1:311320/1‑148 (MQ=255)                                                                            gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTATTTTTTCTCATCTGTTTTTAGATGC‑G‑AAACATGAAAATTAACAGGGGAAAAAAAAATGGGAATGATTGGCTa                                                        >  2:513936/1‑151 (MQ=255)                                                                            gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGACCATTTTATTTTTTCTCCTCTGTTTTTAAATGC‑G‑AAACATGAAAATTAAAGGGGGGAAAATAAATGGGAAAGATTTGCTa                                                        >  2:264243/1‑151 (MQ=255)                                                                                       ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAGATGC‑G‑AAACAAGAAAATTAACGGAGGAAAAAAAAATGGGAATGATTGGCTATTTTGCAGaaa                                             >  1:486989/1‑151 (MQ=255)                                                                                         gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAACGAGATGCACATTTTTTTTTTCCTTATCTGTTTTTAGAAAT‑G‑GAAAATGAAAATTAAAGGGGGGAAAATAAAAGGGGAAGATTGGGTATTTTTCCGaaaat                                           >  2:215690/1‑149 (MQ=255)                                                                                         gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTAAGAAGC‑G‑AAAAATGAAATTTAACGGAGGGAAAAAAAATGGGGATGATTGGCTATTTTGCAGAAAtt                                           >  1:565753/1‑151 (MQ=255)                                                                                         gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAAAGAAAAGAAAATTTTATTTTTTCTTATCTGTTTTAAGAAGA‑G‑AAAAATGAAATTTAAAGGAGGAAAAAAAAATGGGAATGATTGGGTATTTTTTAGAAAtt                                           >  2:169274/1‑151 (MQ=255)                                                                                          ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAACGAAAAACACATTTTATTTTTTCTCATCTTTTTTTAGAAGC‑G‑AAAAATGAAAATTAAAGGAGGGAAAATAAATGGGAATGATTGGGTGTTTTTGAGaaaatt                                          >  2:147617/1‑148 (MQ=255)                                                                                          ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCAAATTTTTTTTTTCCTCCTCTGTTTTTAGATGC‑G‑AAACATGAAAATTAACGGGGGGAAAAAAAATGGGGATGATTTGGTATTTTTCAGAAAttt                                          >  2:147603/1‑150 (MQ=255)                                                                                            ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGa                                                                                                            <  1:406723/85‑1 (MQ=255)                                                                                                 acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAACACAAGCACATTTTTTTTTTTCTCCTCTGTTTTTAGATGG‑G‑AAAAAAGAAATTCAAAGGAGGGAAAATAAATTGGGATGATTGGGTATTTTTCAGAAAATGACTCAGa                                   >  2:370766/1‑151 (MQ=255)                                                                                                    gTAAATAATAAAAATTTCTCGAGGCGGGTTCAAAAAAAAAGACATGCACATTTTTTTTTTTCTCATCTGGTTTTAGATGA‑G‑AAAAATGAAAATTAACGGAGGGAAAATAAATGGGGAAGAATTGGTATTTTTCAGAAAATGAATCAGaaaa                                >  2:630869/1‑151 (MQ=255)                                                                                                       aataatAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGAt                             <  2:170603/151‑1 (MQ=255)                                                                                                            taaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                        <  1:436331/151‑1 (MQ=255)                                                                                                            taaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                        <  1:256653/151‑1 (MQ=255)                                                                                                                       cGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAg             <  2:505879/151‑1 (MQ=255)                                                                                                                               gTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGa     >  1:125236/1‑151 (MQ=255)                                                                                                                                 ccAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa   <  1:354218/151‑1 (MQ=255)                                                                                                                                  cAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaa  >  1:565068/1‑151 (MQ=255)                                                                                                                                          |                                                                                                                                                 GTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGC‑G‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAA  >  NC_000913/2386725‑2387004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑