Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,157,289	0	A	G	47.7%	38.4 / 14.4	44	G171G (GGA→GGG)	ycfH	putative DNase
Reads supporting (aligned to +/- strand):  ref base A (8/15);  new base G (20/1);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 2.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAAGAGAAT  >  NC_000913/1157140‑1157433                                                                                                                                                      |                                                                                                                                                  gaactgaacAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAAATAATCGATTTCTGAt                                                                                                                                                  >  2:597937/1‑151 (MQ=255)      gaacAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGGCGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAATTACTTGGTCTCGGGtttttt                                                                                                                                             >  1:844867/1‑149 (MQ=255)       aacAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGAGACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTAc                                                                                                                                             <  1:410337/150‑1 (MQ=255)            gCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTATTGGGTTTCGGGTTTTACAtctc                                                                                                                                        >  2:137894/1‑150 (MQ=255)                gTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGGTCTCGGGTTTTACATCTCCtttt                                                                                                                                   >  2:289947/1‑151 (MQ=255)                gTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTTGGTCGCGGGTTTTAAATCTCCtttt                                                                                                                                   >  1:116112/1‑151 (MQ=255)                       tCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTAAATCTCCTTTTCCGGCAt                                                                                                                            >  2:623968/1‑151 (MQ=255)                              ccGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GAcc                                                                                                                    <  1:688489/151‑1 (MQ=255)                              ccGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTAATGGGTCTCGGATTTTATATCTCCTTTTTCGGCATTGT‑GAc                                                                                                                     >  2:588278/1‑150 (MQ=255)                                 tGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTc                                                                                                                 <  1:168131/151‑1 (MQ=255)                                     gCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTACATCTCCTTTTTCGGCATTTT‑GACCTTCCGTa                                                                                                             >  2:421266/1‑151 (MQ=255)                                         gTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATgg                                                                                                         >  2:424552/1‑150 (MQ=255)                                          tGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGc                                                                                                         <  2:290573/150‑1 (MQ=255)                                                      ggCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTAATGGATCTCGGGTTTTAAAACTCCTTTTCCCGCATTTT‑GACCCTCCCTAATGCGGAGCAAATgcg                                                                                             >  2:751235/1‑150 (MQ=255)                                                       gCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTgcgc                                                                                            <  2:624419/150‑1 (MQ=255)                                                                cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTgcgc                                                                                  <  1:194400/151‑1 (MQ=255)                                                                       aaaaaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt                                                                           <  2:372493/151‑1 (MQ=255)                                                                        aaaaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTc                                                                          <  1:751225/151‑1 (MQ=255)                                                                          aaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTccc                                                                        <  1:766974/151‑1 (MQ=255)                                                                                cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTAAATATCCTTTTCCCGGATTTT‑GGCCTTCCGTAATGCGGAGCAAATGCGCGATGCTGCGCGGTTTTTCCCCCTggg                                                                  >  2:463507/1‑150 (MQ=255)                                                                                  gATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGAt                                                                 <  2:649521/150‑1 (MQ=255)                                                                                              gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATGGAGATCGGGTTTTACATCTCCTTTTTCGGGATTGT‑GTGCTTCCGTAATGCGGAGCAAATGCGCGATGCTGCGCGGTATGTTCCCCCGGATCGGttgttggggg                                                    >  2:238799/1‑143 (MQ=255)                                                                                              gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGGTTTGTTCCCCTGGATCGGTTACtggggg                                                    >  2:615520/1‑151 (MQ=255)                                                                                              gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTACAACTCCTTTTTCCGCATTTT‑GACCTTCCGTAATGCGGGGCAACCGCGCGATGCTGCGCGGTATGTTCCCCCGGATCGGGTACtggggg                                                    >  2:619888/1‑151 (MQ=255)                                                                                              gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATTGGTCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTTCGTAATGCGGGGCAACCGCGCGATGCTGCGCGGTTTGTTCCCCCGGATCgggttattgggg                                                    >  1:594513/1‑141 (MQ=255)                                                                                                actacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTACATCTCCTTTTCCGGCATTGT‑GACCTTTCGTAATGCGGAGCAACTGCGCGATGCTGCGCGGTATGTCCCCCTGGATCGGGTAATGGTGGaa                                                  >  1:819374/1‑151 (MQ=255)                                                                                                 ctacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGGTCGGTTACTGGGGGgaa                                                 >  2:105644/1‑151 (MQ=255)                                                                                                   acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCAAATGCGCGATGCTGCGCGTTATGTCCCCCTGGGTCGGGTACTGGTGGgaa                                                 >  2:451347/1‑149 (MQ=255)                                                                                                      cTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGGTCTCGGGTTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCCACTGCGCGATGCTGCGCGTTTTGTCCCCCCGGATCGGTTACTGGGGGGAACTGa                                             >  2:218351/1‑150 (MQ=255)                                                                                                      cTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTATCGGATCTCGGGTTTTTTATTTCCTTTTTCGGGCTTGTGGACCTT‑CGTAATGCGGGGCAAATGTGCGGTGCTGCGCGTTATGTCCCCCCGGATCGGTTTATGGGGGGAAATGa                                             >  1:514009/1‑150 (MQ=255)                                                                                                        gTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTTGATCTCGGGTTTTACATTTCCTTTTTCGGCATTGT‑GGCCTTCCGTAATTCGGGGCAACTGCGCGATGCTGCGCGTTATGTCCCCCCTGATCGGTTACTGGTGGGAACTGACta                                          >  2:869680/1‑150 (MQ=255)                                                                                                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTACATCTCCTTTTCCGGCCTTGT‑GAACTTCCGTAATGCGGGGCAACCGGGCGGTGCTGCGCGTTTTGTCCCCCCCGGTCGGGTACTGGGGGGAACTGACt                                           >  2:259917/1‑150 (MQ=255)                                                                                                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGATTTTAAATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAAATGACt                                           >  2:353496/1‑150 (MQ=255)                                                                                                        gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATCTCGGGTTTTAAATCTCCCTTTTCGGCAGTTT‑GAACTTCCGTAATGCGGGGGAACTGCGCGATGCTGCGCGTTTTGTCCCCCCGGATCGGTTACTTGTGGGAAATGACt                                           >  1:75783/1‑150 (MQ=255)                                                                                                                 agGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCaccttacc                                  <  1:213522/150‑1 (MQ=255)                                                                                                                  ggACAGAGAAACGGCGGGGAAAATAATGGATCTCGGATTTTACATCTCCTTTTTCGGCATTGT‑GACCTTCCGTAATGCGGAGCAAATGCGCGATGCTGCGCGTTTTGTCCCCCCTGATCGGGTACTGGGGGGAACTGACTCaccttacctt                                >  1:379242/1‑151 (MQ=255)                                                                                                                   gACAGAGAGACGGGGGGTAAATTTCTGGATCTCGGGTTTTACATCTCCTTTACCGCCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCgccttacct                                 <  1:427511/149‑1 (MQ=255)                                                                                                                       gagaAACGGCGGGGAAAATACTTGGTCTCGGATTTTACATCTCCTTTTTCGGCAATGT‑GACCTTCCGTAATGCGGAGGAACTGCGCGATGCTGCGCGGTTTGTCCCCCCGGAACGGGTACTGGTGGGAAATGACTCCCCCTTCCTTgcgc                            >  1:556514/1‑150 (MQ=255)                                                                                                                                 gggTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTgcgc                            <  1:695892/140‑1 (MQ=255)                                                                                                                                 gggTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTgcgc                            >  2:695899/1‑140 (MQ=255)                                                                                                                                      aaTTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAgg            <  1:323315/151‑1 (MQ=255)                                                                                                                                        ttACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGaa          >  2:665595/1‑151 (MQ=255)                                                                                                                                              gATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAagag     <  2:346842/150‑1 (MQ=255)                                                                                                                                                tctcGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAAGAGAAt  <  2:844878/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGT‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAAGAGAAT  >  NC_000913/1157140‑1157433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑