Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,581	0	G	T	53.3%	21.5 / 43.1	32	D133Y (GAT→TAT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (6/8);  new base T (16/0);  total (24/8)
Fisher's exact test for biased strand distribution p-value = 5.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-05

TATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCG  >  NC_000913/2151431‑2151727                                                                                                                                                       |                                                                                                                                                      tATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATGCGCGCGGGGATAAAATTTACGGCGGTTATACCATTg                                                                                                                                                       <  1:487663/151‑1 (MQ=255)    tCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATTTATACGCTCGAGGATAAAATTTACGGCGGTTATACCATTGATc                                                                                                                                                    <  1:722241/151‑1 (MQ=255)             aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAAGTTACGGCGGTTATACCATTGATCCTTTA‑TTgg                                                                                                                                          <  1:761449/151‑1 (MQ=255)               gTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTA‑TTGGtg                                                                                                                                        >  2:148406/1‑150 (MQ=255)                        cGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATcc                                                                                                                                <  1:368991/150‑1 (MQ=255)                                gCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATTCTTTT‑TTTTTCACCTATTCAAaaaaaga                                                                                                                       >  2:493696/1‑151 (MQ=255)                                       cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATATCATTGATCCTTTT‑TTTTTTACCTATCCAAAAAAAAAAACcgca                                                                                                                >  1:611234/1‑149 (MQ=255)                                       cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACACTTTTTTCTTTT‑TTTTTTACCCATCCCAAAAAAAAAACcga                                                                                                                 >  1:684355/1‑150 (MQ=255)                                         cTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTTACAATATTCCTTTT‑TTTGTTAACTATCCAAaaaaagaaaacggcga                                                                                                              >  1:493165/1‑143 (MQ=255)                                                 gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTATTCTTTT‑TTTGTGACCCATCCCAAAAAAGAAAACGACGAGGTAaaag                                                                                                      >  1:16338/1‑151 (MQ=255)                                                       aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTT‑TTTGTTACCTATCCAAAAAAAGAAGACGACGAGCTAAGAG‑Caaaaa                                                                                               >  2:42793/1‑150 (MQ=255)                                                       aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAAt                                                                                               <  2:135126/151‑1 (MQ=255)                                                        aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACCTTTTTCCTTTT‑TTTTTTTTCTACCCAAAAGAAAAAGGCGGCCAGCCAAGGGTCAAAAt                                                                                               >  1:130737/1‑151 (MQ=255)                                                        aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTT‑TTTGTTACCTATCCCAAAAAAGAAACCGACGAGGTAAAAG‑Gaaaaat                                                                                              >  2:44918/1‑149 (MQ=255)                                                         cGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTTTCCTTTT‑TTTGTTACCCATCCAAAAAAAAAAAGCGACGAGCTAAAAG‑CAAAAATa                                                                                             >  2:165896/1‑151 (MQ=255)                                                          gTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAATACATTATTGTTTTATTTGGGCACC‑ATCCAAAAAAAAAAACCGACGAGTAAAAAT‑AAAAATTat                                                                                            >  1:560479/1‑150 (MQ=255)                                                                     ccAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCa                                                                                 <  2:456841/151‑1 (MQ=255)                                                                               aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTata‑t                                                                      <  2:234579/151‑1 (MQ=255)                                                                                             agaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAAACCATTGATCCTTTA‑TTTGTTACCTATCCAAAAGAAGAAGGCGACGAGCTCAAAG‑CCAAAAAAGGTTGCTAAACAAACATTATA‑TTTATTATTTaaaaa                                                        >  2:703136/1‑149 (MQ=255)                                                                                               atatTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTACCATTTATTCTTTT‑TTTGTCACCTATCCAAAAAAAAAAACCGACGAGCTAAGAG‑CAAAAATAATTTGCTAATCAGCCGGTATA‑TTTTTTTTTTaaaaagg                                                      >  2:842927/1‑147 (MQ=255)                                                                                                 atTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACATTTATCCTTTT‑TTTGTCACCTATCCAAAAAAAAAAGACGACGAGCTTAGAG‑CAAAAATAGTTTTCTAATCAGCCGTTATA‑TTTATTGTTTTAACTGGCg                                                    >  1:628046/1‑151 (MQ=255)                                                                                                   ttCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGt                                                  <  2:297351/151‑1 (MQ=255)                                                                                                       gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTATCCTTTA‑TTTGGTACCTATCCAAAAAAAAAAGACGACGAGCTAAGAG‑CAAAAATTATTCGCTAATCAGCCGTTTTATTTTTTTTTTTAAATTGGGGGtgta                                               >  2:640663/1‑148 (MQ=255)                                                                                                           ggATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACCTTTATCCTTTT‑TTTGTTACCCATCCAAAAAAAGAAACCGACGAGCTAAGAG‑CAAAAATAGTTTGCTTATCAGCCGGTTTA‑TTTTTTTTTTTAAATGGCGGGTGGAtttt                                          >  2:425075/1‑149 (MQ=255)                                                                                                            gATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTTACCTTTATCCTTTT‑TTTGTGACCTATCCAAAAAAAGAAAACGACGAGCTCAGAG‑GAAAAATAATTTGCTAAACAACCGGTTTA‑TTTTTTTGTTAAACTGGCGGGTGTAttttt                                         >  1:663880/1‑151 (MQ=255)                                                                                                             aTGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTT‑TTGGTTACCTATTCCAAAAAAGAAGACGACCAGCCAAAAG‑GAAAATTAGTTTTCTTATTAAACGTTATA‑TTTTTTTTTTTAAATGTCggggttttttttt                                        >  1:474596/1‑140 (MQ=255)                                                                                                               gTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATCCTTTT‑TTTGTCACCTATCCAAAAAAAAAAGACGACGAGCTAAGAG‑CAAAATTAGTTTGCTAATCAGCCGGTATA‑TTTTTTTTTTTAAATGGCGGGTGGAtttttttt                                      >  2:43002/1‑148 (MQ=255)                                                                                                               gTATACGCGCGGGGATAAAATTTACGGCGGGTATTAAACATATTCTATT‑TTTGGGGCCCATACAAAAGAAAAAGACGACGAGCTAAAAG‑CAAAAATAGATCCCTAATCACGCGTTATA‑TTTTTTGTTTTAACTGGCgggttatattttttt                                      >  1:534864/1‑138 (MQ=255)                                                                                                                      cgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAc                                <  1:477804/150‑1 (MQ=255)                                                                                                                                      aCGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAactca                >  2:379251/1‑150 (MQ=255)                                                                                                                                           ggTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTg           >  2:693672/1‑150 (MQ=255)                                                                                                                                           ggTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTg           >  1:111233/1‑150 (MQ=255)                                                                                                                                                    aTTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAgcg  >  2:139996/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      TATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTA‑TTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCG  >  NC_000913/2151431‑2151727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑