Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,676	0	A	C	49.2%	78.6 / 27.6	67	V88G (GTG→GGG)	flc	fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base A (20/14);  new base C (0/33);  total (20/47)
Fisher's exact test for biased strand distribution p-value = 3.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTC  >  NC_000913/657526‑657825                                                                                                                                                       |                                                                                                                                                       ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca                                                                                                                                                        <  1:433902/151‑1 (MQ=255)  cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac                                                                                                                                                       >  1:643113/1‑151 (MQ=255)     ttAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac                                                                                                                                                    >  1:542837/1‑151 (MQ=255)      tAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac                                                                                                                                                    <  1:1126571/150‑1 (MQ=255)        aTCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTc                                                                                                                                                 >  1:1226064/1‑151 (MQ=255)             cGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑g                                                                                                                                           <  2:1233286/151‑1 (MQ=255)               ggTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑Gaa                                                                                                                                         <  2:773815/151‑1 (MQ=255)                   ttttttGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAAt                                                                                                                                      <  2:1033250/150‑1 (MQ=255)                         ggTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTgag                                                                                                                                <  2:622998/150‑1 (MQ=255)                              ttAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAgg                                                                                                                            <  1:1106471/149‑1 (MQ=255)                              ttAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAg                                                                                                                             <  2:38051/148‑1 (MQ=255)                               tAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGgttg                                                                                                                         >  1:113778/1‑151 (MQ=255)                               tAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGgtt                                                                                                                          <  1:781369/150‑1 (MQ=255)                                   gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTa                                                                                                                      <  2:1079692/150‑1 (MQ=255)                                    tgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGgttgtt                                                                                                                       >  1:1024084/1‑148 (MQ=255)                                      cGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGAcc                                                                                                                  <  1:898137/151‑1 (MQ=255)                                       ggTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCg                                                                                                                 >  1:1023890/1‑151 (MQ=255)                                       ggTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCg                                                                                                                 >  1:419130/1‑151 (MQ=255)                                             ggCCGAAAACAGCCAAAATGCCAGGGCGGTCATGCCAAAAGCCCCCAAAAGGTTGACAAAAACGTTCAGAAATGCCCAGCAAAAGCGGCCCTCTTGTAACAAAACCACCCCTTCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACaa                                                                                                           <  2:232043/151‑1 (MQ=255)                                              gCCAAAAACACCCAGAATGCCAGTGCGGTCATGGAAAAAGCCCCCAGAAGGTTGACAAAAACTTTAAGTAATGCCCAGCCAAAGCGGCCCTCTTGAAAAAAAAACCCCACTTCGGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACaaa                                                                                                          <  2:83972/151‑1 (MQ=255)                                                      cAGCCAGAATGCCAGTGCGGTCATGGAAAAAGCCCCCAGAAGGTTGACGAAACCGTTCAGTAATCCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtg                                                                                                  <  1:857452/151‑1 (MQ=255)                                                        gCCAAAATGCCAGTGCGGTCATGCCAAACGCCCCCAGAAGTTTGACAAAACCTTTCATTAATCCCCAGCCAAAGCGCCCCTCTTTTACCAAACACCCCCCTCCTCCC‑AAAAATTTTGAAAGGTTTTTTAGCCCGCCACAAAATCCggtggt                                                                                                <  2:181882/151‑1 (MQ=255)                                                         ccAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGgtggtg                                                                                               <  1:229908/151‑1 (MQ=255)                                                         ccAGAATGCCAGTGCGGTCAGGGCAAAAGACCCCAGAAGGTTGAAGAAAACGTTCAGTAATCCCCACCCAAAGCGCCCCTTTTGTAAAAAAAACCCCACTCCTGCC‑AAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGgtggtg                                                                                               <  2:1078759/151‑1 (MQ=255)                                                          cAGAAGGCCAGTGCGGTCAGGGAAAAAGCCCCAAGAAGGTTGAAAAAACCGTTCAGTAATCCCCAGCAAAAGCGGCCCTTTTTAAAAAAAAACCCCACTCCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGa                                                                                              <  2:1018232/151‑1 (MQ=255)                                                           aGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAt                                                                                             >  2:171584/1‑151 (MQ=255)                                                            gAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAtt                                                                                            <  1:994949/151‑1 (MQ=255)                                                              aggCCAGTGCGGTCATGGCAAAAGCCCCCAGAGGGTTGACAAAAACGTTCAGAAATCCCCAGCAAAAGCGGCCCTTTTGTAAAAAAACCCCCCCTTCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTaa                                                                                          <  1:831261/149‑1 (MQ=255)                                                              aTGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTa                                                                                           >  2:862031/1‑150 (MQ=255)                                                                gCCAGTGCGGTCATGGCAAAAGCCCCCAGAAGGTTGACGAAACCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAACCCGGTGGTGATTAATa                                                                                        <  1:1333321/151‑1 (MQ=255)                                                                gCCAGTGCGGTCATGGAAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt                                                                                         <  1:193937/150‑1 (MQ=255)                                                                cccAGTGCGGTCGGGACAAAAGCCCCCAGAAGGTTGAAAAAAACGTTCAGTAATGCCCAGCAAAGGCGGCCCTTTTGTAACAAAACCCCCACTCCTGCA‑GAAAGTTTAGAAAAGTTTGTTGGACCGCCACAAAATCCGGTGGTGATTAATa                                                                                        <  2:98655/150‑1 (MQ=255)                                                                  cAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACt                                                                                      >  2:1034988/1‑151 (MQ=255)                                                                   ggggCGGTAATGGAAAAAGCCCCAAGAAGTTTGACAAAAACGTTAAGTAATGCCCAGCAAAGGCGGCCCTTTTGAAAAAAAACCCCCACTCCTGCG‑AAAAATTTTGAGAGGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACtt                                                                                     <  2:779395/148‑1 (MQ=255)                                                                    ggggggTAATGGCAAAAGCCCCAAGAGGGTTGAAAAAACCGTTCAGAAATCCCCACCCAAGGCGGCCCTTTTGTAAAAAAACCACCACTTCGGCG‑GAAAATTTTGAAAATGTTTTTACACCGCCACAAAATCCGGTGGTGATTAATACttt                                                                                    <  2:170761/147‑1 (MQ=255)                                                                     tGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc                                                                                   >  1:790241/1‑151 (MQ=255)                                                                     tGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc                                                                                   >  1:777137/1‑151 (MQ=255)                                                                     ggCGGTAATGGCAAAAGCCCCCAGAAGGTGGACAAAAACGTTCAGTAAGGCCCAGCAAAGGCGGCCCTCTTGAAAAAAAACCCCCACTTCGGCG‑AAAAATTTTGAGAAGTTTGTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc                                                                                   <  2:1267899/150‑1 (MQ=255)                                                                      gCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc                                                                                  >  2:1008034/1‑151 (MQ=255)                                                                      gCGGTCATGGAAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATCCCCAGCAAAAGCGGCCCTCTTGTAAAAAAAACCCCACTTCTGCC‑GAAAATTTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc                                                                                  <  2:1029568/151‑1 (MQ=255)                                                                          tCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                              >  1:1009363/1‑151 (MQ=255)                                                                           aagggaaaaaGACCCCAAAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTTTTGTAAAAAAACCCCCACTTCTGCC‑GAAAATGTTGAGAAGTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACt                                                                             <  2:167172/145‑1 (MQ=255)                                                                              ggCAAAAGCCCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGAAACAAAAACACCACTTCGGCG‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGa                                                                          <  1:1254976/151‑1 (MQ=255)                                                                                 aaaaGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATc                                                                        >  1:818836/1‑150 (MQ=255)                                                                                 aaaaGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATc                                                                        >  2:1264001/1‑150 (MQ=255)                                                                                                   gACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCctgct                                                      <  1:228739/150‑1 (MQ=255)                                                                                                     aaaaaaCTTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAAAAAACCCCCACTCCTGCC‑AAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGaa                                                   <  2:1112079/149‑1 (MQ=255)                                                                                                       aaaaCTTCCAGTAATCCCCAGCAAAAGCGCCCTTCTTG‑AAAAAAAACCCCCCCTCCGCCAAAAAATTTTGAGAGGTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAcc                                                 <  2:1242826/151‑1 (MQ=255)                                                                                                        aaaCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAAAAAAACCCCACTTCGGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCa                                                <  1:98583/151‑1 (MQ=255)                                                                                                          actttCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTAAAAAAAACCCCCCTTCGGCC‑GAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATg                                              <  1:327321/148‑1 (MQ=255)                                                                                                          actttCAGTAAGGCCCACCCAAAGGGGCCCTCTTGTAAAAAAACCCCCACTCCTGCA‑AAAAAGTTGGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATg                                              <  2:29546/148‑1 (MQ=255)                                                                                                           cGTTCAGTAATGCCCAGCAAAAGCGGCCCTTTTGTAAAAAAAACCCCACTTCTGCC‑GAAATTGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGc                                             <  1:71159/151‑1 (MQ=255)                                                                                                           cGTTCAGAAATGCCCAGCAAAAGCGCCCCTCTTGTAACAAAAACCCCCCTTCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGc                                             <  1:139414/151‑1 (MQ=255)                                                                                                            gTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCg                                            >  2:1304029/1‑151 (MQ=255)                                                                                                             ttCAATAATCCCCAGCCAAAGCGGCCCTTTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGc                                             <  1:290138/149‑1 (MQ=255)                                                                                                                aGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtc                                        <  1:845227/151‑1 (MQ=255)                                                                                                                  aaaTCCCCAGCCAAAGCGGCCCTCTTGAAACAAAACCCCCACTTCTGCC‑AAAAATGTTAAAAGGGTTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcct                                      <  2:1021538/150‑1 (MQ=255)                                                                                                                   aacgCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcct                                      <  1:1113321/147‑1 (MQ=255)                                                                                                                   aaTGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcct                                      >  2:220952/1‑150 (MQ=255)                                                                                                                   aaTGCCCAGCCAAAGCGGCCCTCTTGAAACAAAAACCCCACTTCTGCC‑AAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcct                                      <  1:1040277/150‑1 (MQ=255)                                                                                                                      gCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                  <  1:379052/151‑1 (MQ=255)                                                                                                                        ccAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcct                                >  1:693135/1‑151 (MQ=255)                                                                                                                         cAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCCCTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta                               <  1:346518/151‑1 (MQ=255)                                                                                                                            ccAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatg                             >  1:1175877/1‑150 (MQ=255)                                                                                                                                aGCGGCCCTCTTGTAACAAAAACCCCCCTCCCGCC‑GAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGaa                        <  1:638834/151‑1 (MQ=255)                                                                                                                                      ccTCTTGTAACAAAAACCCCACTTCTGCC‑GAAATTGTTGAAAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGcccc                  <  1:340435/151‑1 (MQ=255)                                                                                                                                          ttGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATc              >  1:948812/1‑151 (MQ=255)                                                                                                                                           tgaaaCAAAAACCCCACTTCGGCC‑AAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAAt               <  2:143190/146‑1 (MQ=255)                                                                                                                                               aCAAAAACCCCACTTCTGCC‑AAAAATGTTAAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGtt         <  1:358421/151‑1 (MQ=255)                                                                                                                                                aaaaacCCCCACTTCTGCG‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGttt        <  2:264572/150‑1 (MQ=255)                                                                                                                                                    aaCACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTg    >  2:732613/1‑151 (MQ=255)                                                                                                                                                       accacTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTc  >  1:139777/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                       CCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCC‑GAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTC  >  NC_000913/657526‑657825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑