Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	48.1%	72.1 / 49.7	81	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (3/39);  new base T (39/0);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 6.49e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583                                                                                                                                                       |                                                                                                                                                      cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                                       <  2:654685/151‑1 (MQ=255)   gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                     <  2:200525/151‑1 (MQ=255)    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCa                                                                                                                                                     >  1:226012/1‑150 (MQ=255)        tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACa                                                                                                                                                 >  1:867442/1‑150 (MQ=255)          tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAaaaaaa                                                                                                                                               >  2:980167/1‑150 (MQ=255)           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAt                                                                                                                                              <  2:711061/150‑1 (MQ=255)                 aCATTTCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                       <  1:585144/151‑1 (MQ=255)                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  2:105590/151‑1 (MQ=255)                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  1:906728/151‑1 (MQ=255)                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  1:569158/151‑1 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAGACAAATACaaaaaa                                                                                                                                      >  1:1344917/1‑149 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACt                                                                                                                                     <  2:663005/151‑1 (MQ=255)                    ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAcaaaaacaaaaaatt                                                                                                                                    >  2:1299414/1‑148 (MQ=255)                      gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                  <  2:764153/151‑1 (MQ=255)                      gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                  <  1:1213838/151‑1 (MQ=255)                           tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                              <  1:1349203/150‑1 (MQ=255)                             ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATTGAGGTggc                                                                                                                            >  1:218619/1‑148 (MQ=255)                             ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa                                                                                                                           <  1:919808/151‑1 (MQ=255)                                 ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCatgat                                                                                                                       >  1:1363566/1‑151 (MQ=255)                                   ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTCAGAAAAAAAAAAAAAATGGAGTTGCCatgttg                                                                                                                      >  1:247089/1‑150 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAAAAGAGTTGCCATGATGA‑gg                                                                                                                  >  2:1175096/1‑150 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑Gc                                                                                                                  <  2:1083030/151‑1 (MQ=255)                                        gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGaa                                                                                                               <  1:825549/151‑1 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCCAAAAAAACAAAAAAAAGAGTTGCCGTGATGA‑GGGaaa                                                                                                              >  2:716655/1‑150 (MQ=255)                                          aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCCTGATGA‑ggggaa                                                                                                              >  1:904587/1‑145 (MQ=255)                                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGt                                                                                                            <  2:299912/151‑1 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTTGCATGATGA‑GGGGAAgaa                                                                                                           >  1:180955/1‑148 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGta                                                                                                           <  1:100004/150‑1 (MQ=255)                                               tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATTGAGGTGCCATGATGA‑GCGAAAGgaat                                                                                                         >  1:581575/1‑150 (MQ=255)                                               tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGtaat                                                                                                         <  1:1170902/150‑1 (MQ=255)                                               tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGAATTTTTCACACAAAAACAAAAAATAGAGTTGGCATTGTGA‑GGGGAAGtaaa                                                                                                         >  2:1357325/1‑149 (MQ=255)                                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGtaataa                                                                                                       <  1:13667/151‑1 (MQ=255)                                                 attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGATGA‑GCGAAAGtaataa                                                                                                       >  1:158444/1‑150 (MQ=255)                                                   tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAAAGGAGGTGGCCTGATGA‑GCGGAAGgaataaaa                                                                                                     >  2:1256785/1‑148 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCATGATGA‑GCGAAAGGAAAAAACaaaaagc                                                                                              >  1:1253056/1‑145 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGc                                                                                              <  1:1165342/150‑1 (MQ=255)                                                              cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGa                                                                                         <  1:1165471/151‑1 (MQ=255)                                                               gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGA‑GCGAAAGTAATAAACAAAAAGCAGTGa                                                                                         >  2:135344/1‑150 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGAGGTGCCATGATGA‑GCGAAAGGAAAAAACAACAAGCAGGGaaa                                                                                       >  1:474772/1‑149 (MQ=255)                                                                  gATTCACGAACTGTTAATTACATATTCACGGTGTCAAAAAATATAAAACCACATTTTTAGTTGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  2:810522/150‑1 (MQ=255)                                                                  gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                     <  1:776093/151‑1 (MQ=255)                                                                  gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  2:451981/150‑1 (MQ=255)                                                                  gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  2:1062406/150‑1 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                    <  2:913683/151‑1 (MQ=255)                                                                    ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGGATTTTTTCATAAAAAAAAAAAAAAAAGAGTTTGGATGCTGA‑GGGAAAATAATAAAAAAAAGGGAGTGacga                                                                                      >  2:219562/1‑145 (MQ=255)                                                                     tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATGA‑GCGAAAGAAATAAACAACAAGCAGGGAATAAGtt                                                                                  >  1:893060/1‑151 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATGGAGGTGCCATTATGA‑GCGAAAGAAATAAACAAAAGGCAGTGAAAAAGATg                                                                                 >  1:100275/1‑151 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                 <  1:750136/151‑1 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGTCATGATGA‑GCGAAAGTAATAAACAAAAAGCAGAGAATAAGATGAc                                                                               >  2:643624/1‑150 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                              <  1:1031720/151‑1 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAATTGAGTTGGCCTGATGA‑GCGGAAAGAATAAACAACAAGCAGGGGAAAAATTGAc                                                                               >  1:595387/1‑150 (MQ=255)                                                                            cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATGGAGTTGGCATGATGA‑GCGGAAGGAATAAGAAACAGGCAGTGAATAAGTTGACaa                                                                             >  2:497240/1‑148 (MQ=255)                                                                            cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATAAAAAAAATGGAGGTGTCCTGATGG‑GCGGAAGTAATAAAAAAAAAGCAGTGAATAAGTTGACag                                                                             >  2:1111292/1‑149 (MQ=255)                                                                                tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GGGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGAttt                                                                       >  2:454359/1‑150 (MQ=255)                                                                                tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATAGGGTTGGCATGATGA‑GCGGAAGTAAAAAAAAACAAGCAGGGAATAAGATGAAAGAGAttt                                                                       >  2:773583/1‑150 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                     >  1:210940/1‑151 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATAGAGTTGGCATGATGA‑GCGGAAATAATAAGAAACAAGCAGGGAATAAATTGACAGAGAATGTCGCTa                                                                 >  2:970261/1‑150 (MQ=255)                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                             <  1:1347612/150‑1 (MQ=255)                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                             <  1:1318687/150‑1 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                            <  1:985039/150‑1 (MQ=255)                                                                                                gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGc                                                       <  1:1299352/151‑1 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGGGTTGCCATGATGA‑GCGGAAGTAAAAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTg                                                   >  1:272741/1‑150 (MQ=255)                                                                                                            aaaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAc                                           <  2:933903/149‑1 (MQ=255)                                                                                                              aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATACAAAAAATGGAGTTGTCATGATGA‑GGGGAAGTAATAAACAAAAAGGAGTGAAAAAGATTAAAGAGAATGTTGCTAACTTTACCGCCATGAATTTTAccc                                         >  2:587477/1‑151 (MQ=255)                                                                                                               aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCACAAAAATAAAAAAAATGGAGTTGTCCTGATGA‑GGGAAAATAATAAACAACAGACAGGGAATAAAATGAAAGAGAATGTTGCTAAATTTTACGCCATGATTTTTAcccc                                        >  2:280094/1‑150 (MQ=255)                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCCACCAAAAAAAAAAATTGGGGTGCCCTGGTGA‑GGGGAAGGAATAAACAAAAAGCAGGGAAAAAATTTAAAGAGATTGTTGCTAACTTTTCCCCCCTGATTTTTAccccca                                      >  2:666867/1‑147 (MQ=255)                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                     <  1:183284/151‑1 (MQ=255)                                                                                                                   cacaTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGGGGTGCCATGATGA‑GGGAAAGGAAAAAACAAAAAGCAGTGAAAAAATTGACAGAGAATGTCGCTAACTTTACCGCCCTGATTTTTAACCCaa                                      >  1:279122/1‑149 (MQ=255)                                                                                                                       tttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGA‑GCGAAAGAAATAAACAAAAAGGAGGGAAAAAGTTGACAGAGATTGTCGCTAACTTTAACGCCCTGATTTTTACCCGAATGCCtg                                >  1:779176/1‑151 (MQ=255)                                                                                                                       tttttAGTGGTAGTTTGTGGCGGGGGATTTTTTCCGACCAAAACAAAAAATGGGGTTGGCATGGTGAGGGGAAAGAAAAAAAAAAAAGG‑AGGGGAAAAATTGAACGAGATTGTTGCTAACTTTAACGCCCTGATTTTTTCCCGCATGCCtg                                >  2:1070931/1‑151 (MQ=255)                                                                                                                         tttAGTGTTATTTTGTGGCGGTGATTTTTCCAAAAAAAATACAAAAACTGGAGTTGCCAGGATGA‑GCAAAAGAAATAAGAACCAGGAAATGAATAAGTCAACAGGGATTTTCGCTAAATTAACCGCCATGATTTCTACCCGAATGCCtga                               <  2:1364966/150‑1 (MQ=255)                                                                                                                           tAGTGGTATTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                             <  1:535910/150‑1 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGGGGTGGCATGATTA‑GGGAAAGGAAAAAAAAAAAAGCAGGGAATAAAATGACAGAGATTGTTGCTAAATTTAACGCCATGATTTTTACCCCAAAGCCcgatg                             >  2:591625/1‑150 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAATGGGGTTTGCCTGATGA‑GGGAAAAAAATAAACAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCCAAAGCCtggtga                            >  1:968835/1‑150 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGCCATGATGA‑GCGAAAGAAATAAACAAAAAGCAGGGAATAAAGTGAAAGAGATTGTTGCTAACTTTTCCGCCATGATTTTTACCCGAAAGCCTGATGAc                           >  1:239556/1‑151 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                           <  2:226002/151‑1 (MQ=255)                                                                                                                                  aGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAACTGGAGTTGCCATGATGA‑GGGGAAGGAAAAAACAACAAGGAGTGAATAAAATGACAGAGAATGTCGCTAACTTTACCGCCCTGAATTTTACCCGAATGCCtgatgaagagggg                     >  2:780286/1‑144 (MQ=255)                                                                                                                                    tttGTGGCGGTGAATTTTTCCAGAAAAAAAAAAAAACGGGAGTTGCCATGAGGA‑GCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                    <  2:332560/150‑1 (MQ=255)                                                                                                                                      ggtgGCGGTAAATTTTTCCAGAAAAATACAAAAACGGGAGTGCCCATGATGA‑GGAAAAGTAATAACCAACGGCCAGTAAATAGGTTGAAAGGCATTGTCGCTACCTGTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  2:290951/149‑1 (MQ=255)                                                                                                                                             gTGAATTTTCCCAAAAAAAAAAAAAAACGGGATTTGCAATAATGA‑GCAAAAGTAAAAAGAAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGTTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          <  2:806322/151‑1 (MQ=255)                                                                                                                                               gAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:563896/150‑1 (MQ=255)                                                                                                                                                      tCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:1287970/1‑150 (MQ=255)                                                                                                                                                       ccAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:1254082/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206285‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑