| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,578,143 | 0 | A | C | 57.1% | 4.6 / 12.3 | 14 | L79V (TTA→GTA) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (1/7); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02 | |||||||||||
AATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGT > NC_000913/2578018‑2578287 | aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAACCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCCCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTACCCCCA‑GCACCGCCGTACCGTTagag < 2:62506/151‑1 (MQ=255) aaaTTTGTCCGGGTCGAGTTCGTCAACTTAAATGTCAAATCCATCAACCCCGGCGAATTTCTTAAACAGAACGCCTTTGCCTTCCACCCCCGGTTTGCCTGCCGGCGCGCCAATGTTGCCTACCCCCA‑GCACCGCCGTACCGTTAGAGATc < 1:430342/151‑1 (MQ=255) gTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg > 2:359772/1‑151 (MQ=255) taaatcaCAGCAACCCCGGACAATTTCTTAACAATCCCCCTCGTGCCTCCCATCCCTGGTTGGCCTTCCGGCGCCCCAATTGCGATTACCCCAA‑GCTCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 1:429419/144‑1 (MQ=255) taaaaTACATCAACCCCGGCGAATTTCTAAAACAGAACGCCCTTCCCTCCCATCCCCGGTTTGCCTGCCAGCGCGCAAATGTTCCCAACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 2:374175/148‑1 (MQ=255) aTACATCACCCCCGGCAAATTTTTTAAACAGAACGCCTTGGCCTTCCATCACCGTTTGGCCTGCCGGCGCGCCAATGTTGCCTAACCCCC‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc < 2:509575/151‑1 (MQ=255) aTACATCAACCCCGGCGATTTTCTTAAACAGACCCCCTTCCCCCCCCACCCCCGGTTTGCCTCCCGCCGCGCCAATGTTCCCTACCCCCA‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc < 1:164293/151‑1 (MQ=255) gCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt > 2:78598/1‑151 (MQ=255) ttcttcAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGACAATGTTGCCTACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGa > 1:532847/1‑151 (MQ=255) ccacccccGGTTTGCCTCCCAGCGCGCCATTGTTGCCTACCCCCA‑CCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGcc < 1:491725/145‑1 (MQ=255) cgttttGCCTGCCAGCGCCCCAATTTTGCCTACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAg < 2:338135/148‑1 (MQ=255) ccctgccAGCGCGCAATTGTT‑CCCAACCCCAGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGcc < 1:194767/150‑1 (MQ=255) tGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTgtgt > 1:178757/1‑151 (MQ=255) tGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTgtgt > 1:511157/1‑151 (MQ=255) | AATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGT > NC_000913/2578018‑2578287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |