| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,285,877 | 0 | C | G | 55.6% | 4.6 / 10.8 | 9 | P134A (CCG→GCG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base G (5/0); total (6/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
AAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAA > NC_000913/3285727‑3286009 | aaaGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGc < 2:304807/151‑1 (MQ=255) ccgttAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGAATTTTTCCTCCTTTTCCACCCCGGGAAACCTGCTGGGGGCGCGTCTTTTTTTTTCCGt > 1:208416/4‑151 (MQ=255) gCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGCGGGGAATTTG‑TTATTCTTTTCCCTCCAGGGGAAACCGCTGGGGGCGGGTTTTTTTTTTCTCGTTTTCCTg > 1:208325/1‑149 (MQ=255) aaGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGGTTTGGTCATCCTTTGCCCGCCAGGGGAACCCGCTGGGGGCGGTTTTATTTTTTGCCGTTTTTCTGCTTAtttt > 1:146888/1‑151 (MQ=255) gTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTTCTCCTCATTTTTCCGCCAGGGGAACCTGCTGGGGGCGGTTCTTTTTTTTTCCGTTTTCCTGcttttctttt > 2:33833/1‑151 (MQ=255) tGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGGGTTTTGTTATCCTTTGTCCGCCCCGGAAACCCTCTCGGGGCGATTCTATTTTTTTCCTTTTTTCTGCCTATTTTTTTTCTtcg > 1:200986/1‑151 (MQ=255) atgcgatTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTc < 1:405001/151‑1 (MQ=255) cgatTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAg < 1:192295/150‑1 (MQ=255) ttCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCg < 2:283250/151‑1 (MQ=255) agGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCaa > 2:67868/1‑150 (MQ=255) | AAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACTTTGTTGCCGATTATGGC‑GGGAATTTGCTCATCATTTGCCAGCCAGGGAAACCTGCTGGGGCCGATTCTATTTTTCGCCGTTTACCTGCTTATCTTTTTCCTGCGCGTCGGCTGGACCCACGTCGGTTATTCAGTCGGCGTGAAGGCGATCGATAAAGTGCGAGAGAACTCGCAGATGATTGCCCGTTCGGCAA > NC_000913/3285727‑3286009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |