BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,347,989	G→A	5.6%	R38R (AGG→AGA)	cls_1 →	Cardiolipin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,347,989	0	G	A	5.6%	385.8 / 6.7	125	R38R (AGG→AGA)	cls_1	Cardiolipin synthase
Reads supporting (aligned to +/- strand): ref base G (56/61); new base A (5/2); total (62/63)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 126 total aligned reads displayed.

GCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATT  >  USA300TCH1516_ALE/1347851‑1348128

tccTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGTTTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCg                                                                                                                                           >  2:350288/2‑141 (MQ=255)

gCCTTTCTTTATAGGAGCAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCg                                                                                                                                           <  1:282918/141‑1 (MQ=255)

  ctttctttATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGCTTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTa                                                                                                                                         >  2:110283/1‑141 (MQ=255)

   tttctttATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTAc                                                                                                                                        <  2:963770/141‑1 (MQ=255)

      ctttATAGGAGAAAAGACTATGCGATATACATTTTCAAATGATTTAGGAACACTTTTTACCATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGACGTACAGc                                                                                                                                     >  3:47896/1‑141 (MQ=255)

       tttATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCg                                                                                                                                    >  4:109229/1‑141 (MQ=255)

       tttATAGGAGAAAAGACTATGCGATATACATTTTCAAATGATTTAGGAACACTTTTTACCATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGACGTACAGCg                                                                                                                                    >  4:150648/1‑141 (MQ=255)

       tttATAGGAGAAAAGACTATGCGATATACATTCTCAAATGATTTAGGAACACTTTTTACCATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑ATTAGACGTACAGCg                                                                                                                                    >  2:1004228/1‑141 (MQ=255)

        ttATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGa                                                                                                                                   >  2:969533/1‑141 (MQ=255)

        ttATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGa                                                                                                                                   >  3:301878/1‑141 (MQ=255)

         tataGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAg                                                                                                                                  <  1:414968/141‑1 (MQ=255)

         tataGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAg                                                                                                                                  <  2:990605/141‑1 (MQ=255)

          ataGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGt                                                                                                                                 <  2:382605/141‑1 (MQ=255)

           taGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGtt                                                                                                                                >  2:236200/1‑141 (MQ=255)

            aagagaAAAGACTATGCGATTTACATTTTCAAACTATTTAGGAACGTTATTTACGATTATTTTAGCCAGTTGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTc                                                                                                                               <  1:811079/139‑1 (MQ=255)

            aGGAGAAAAGACTATGCGATATACATTTTCAAATGATTTAGGAACACTTTTTACCATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGACGTACAGCGAGTTc                                                                                                                               >  3:157697/1‑141 (MQ=255)

             ggAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCa                                                                                                                              >  2:336855/1‑141 (MQ=255)

              gagaAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCaa                                                                                                                             <  2:805764/141‑1 (MQ=255)

              gagaAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTAGTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCaa                                                                                                                             <  1:573282/141‑1 (MQ=255)

                   aaGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTgg                                                                                                                        <  3:125399/141‑1 (MQ=255)

                       cTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCAt                                                                                                                    >  1:1073302/1‑141 (MQ=255)

                         aTGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATgg                                                                                                                  <  3:287231/141‑1 (MQ=255)

                         aTGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATgg                                                                                                                  >  3:370026/1‑141 (MQ=255)

                         aTGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATgg                                                                                                                  <  2:837421/141‑1 (MQ=255)

                          tGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGc                                                                                                                 >  3:86315/1‑141 (MQ=255)

                          tGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGACTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGCTCAACTTGGGCATGGc                                                                                                                 >  2:99503/1‑141 (MQ=255)

                              aTTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTCtt                                                                                                             >  1:716585/1‑141 (MQ=255)

                              aTTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTAtt                                                                                                             >  2:778922/1‑141 (MQ=255)

                                 tACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGt                                                                                                          >  2:153562/1‑141 (MQ=255)

                                 tACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGt                                                                                                          <  2:602964/141‑1 (MQ=255)

                                 tACATTTTCAAACGAGTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGt                                                                                                          <  2:846028/141‑1 (MQ=255)

                                  aCATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTa                                                                                                         <  1:786709/141‑1 (MQ=255)

                                   cATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTAc                                                                                                        >  1:62628/1‑141 (MQ=255)

                                    aTTTTCANACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCACCTTGTTGTTGGCTATTTGTACt                                                                                                       <  2:817079/141‑1 (MQ=255)

                                     ttttCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACtt                                                                                                      <  1:74240/141‑1 (MQ=255)

                                          aaaCGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGt                                                                                                 <  1:581483/141‑1 (MQ=255)

                                             cGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCtt                                                                                              >  4:417882/1‑141 (MQ=255)

                                             cGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCtt                                                                                              >  2:787340/1‑141 (MQ=255)

                                                tttAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTAcc                                                                                           <  1:1086269/141‑1 (MQ=255)

                                                 ttAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCa                                                                                          <  2:981357/141‑1 (MQ=255)

                                                  tAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCat                                                                                         <  1:725810/141‑1 (MQ=255)

                                                   aGGAACGTAATGTACTAATATTTTAGACATTGGATTCAACATTAATTTAGTATTGGCTGTAATAATTATCTTTTGAGAAAGA‑AATAGGCGTACAGCGAGGTCAACTTGGGCATGGGTATTAGTACTTTTAGTCTTACCatt                                                                                        >  2:335017/1‑141 (MQ=255)

                                                    ggAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCattg                                                                                       <  2:1073097/141‑1 (MQ=255)

                                                     gAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCattga                                                                                      >  3:326285/1‑141 (MQ=255)

                                                       aCGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCattgatt                                                                                    <  2:420084/141‑1 (MQ=255)

                                                         gTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTgg                                                                                  >  2:20897/1‑141 (MQ=255)

                                                         gTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTgg                                                                                  <  2:980103/141‑1 (MQ=255)

                                                          ttATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGt                                                                                 <  1:530658/141‑1 (MQ=255)

                                                              ttACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTa                                                                             >  3:430848/1‑141 (MQ=255)

                                                              ttACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTa                                                                             <  4:40278/141‑1 (MQ=255)

                                                               tACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTAt                                                                            >  3:133291/1‑141 (MQ=255)

                                                               tACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTAt                                                                            >  1:182272/1‑141 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        >  4:45949/1‑141 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        <  2:824803/141‑1 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        >  4:68642/1‑141 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        <  1:949253/141‑1 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        >  2:1047633/1‑141 (MQ=255)

                                                                   attattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCtt                                                                        >  1:284275/1‑141 (MQ=255)

                                                                    ttattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTg                                                                                                           <  2:670056/105‑1 (MQ=255)

                                                                    ttattTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTg                                                                                                           >  1:670056/1‑105 (MQ=255)

                                                                      attTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTAc                                                                     <  4:211605/141‑1 (MQ=255)

                                                                       ttttAGCCTTTGAATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTCTACt                                                                    <  2:119491/141‑1 (MQ=255)

                                                                       ttttAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGTGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACt                                                                    <  1:294423/141‑1 (MQ=255)

                                                                       ttttAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACt                                                                    >  2:828659/1‑141 (MQ=255)

                                                                             ccATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGtttt                                                              >  1:277586/1‑141 (MQ=255)

                                                                             ccATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGtttt                                                              <  1:371255/141‑1 (MQ=255)

                                                                              cATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGttttt                                                             <  3:365252/141‑1 (MQ=255)

                                                                                ttGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTg                                                           >  2:507123/1‑141 (MQ=255)

                                                                                    aTTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAg                                                       <  4:57286/141‑1 (MQ=255)

                                                                                       catcatTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAAc                                                    <  4:211202/141‑1 (MQ=255)

                                                                                        atcatTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAAcc                                                   >  2:60531/1‑141 (MQ=255)

                                                                                        atcatTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAAcc                                                   >  1:698495/1‑141 (MQ=255)

                                                                                        atcatTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAAcc                                                   >  2:568452/1‑141 (MQ=255)

                                                                                         tcatTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCGACTTGGGCATGGCTGGTGGGACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCg                                                  >  1:648404/1‑141 (MQ=255)

                                                                                          catTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGt                                                 <  1:466342/141‑1 (MQ=255)

                                                                                               aTTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCgg                                            <  1:112091/141‑1 (MQ=255)

                                                                                               aTTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGACGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCgg                                            <  4:157697/141‑1 (MQ=255)

                                                                                                   aGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACg                                        <  2:337761/141‑1 (MQ=255)

                                                                                                   aGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACg                                        <  1:401011/141‑1 (MQ=255)

                                                                                                    gTATTGGCTTTTATTATTATCTTTTTTTTTTTT‑TTTTGTCGTACAGCGAGTTCAACGTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCTTTTcttctctt                                       >  4:447684/1‑134 (MQ=255)

                                                                                                          gCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTg                                 <  1:106530/141‑1 (MQ=255)

                                                                                                             tttATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAAt                              >  1:232685/1‑141 (MQ=255)

                                                                                                             tttATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAAt                              <  1:176318/141‑1 (MQ=255)

                                                                                                             tttATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAAt                              <  1:748994/141‑1 (MQ=255)

                                                                                                                   attatCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAAc                        <  2:636096/141‑1 (MQ=255)

                                                                                                                    ttatCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGTTATAACCGTTTCGGCACGCAAATTGAATAAAAACa                       >  2:939602/1‑141 (MQ=255)

                                                                                                                     tatCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTTTTTTTTTTTTGATTTGTTTTATTCTTTACTTTTGTTTTGGTAGATTCGTTTTTGCACGCAAATTGAATAAAATCaa                      >  2:1035452/1‑141 (MQ=255)

                                                                                                                       tCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATg                    <  2:336393/141‑1 (MQ=255)

                                                                                                                       tCTTTCTAAAAAGATAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAAt                     >  3:298909/1‑141 (MQ=255)

                                                                                                                         tttttAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGt                  >  1:914609/1‑141 (MQ=255)

                                                                                                                              agaaaga‑aATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTCCCATCGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACgt             <  3:317249/141‑1 (MQ=255)

                                                                                                                               gaaaga‑aaTAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACgtg            <  2:160207/141‑1 (MQ=255)

                                                                                                                                  aga‑aaTAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTTGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTa         >  1:222814/1‑141 (MQ=255)

                                                                                                                                    a‑aaTAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAAc       <  4:397934/141‑1 (MQ=255)

                                                                                                                                      aaTAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACg      >  3:222298/1‑141 (MQ=255)

                                                                                                                                      aaTAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACg      <  2:1002871/141‑1 (MQ=255)

                                                                                                                                        tAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGa    >  2:488484/1‑141 (MQ=255)

                                                                                                                                         aGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGAt   >  2:501810/1‑141 (MQ=255)

                                                                                                                                         aGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTGTTTGTCTGACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCTGGCAATTTGGATGAAAACAATGGTAACTGGTTAACGGAt   >  2:103024/1‑141 (MQ=255)

                                                                                                                                          ggCGTACAGCGAGTTCAACGTGGGCAGGGCGATGGGTACGTTGTGTCGGACCATTGATTGGTTTTATTCTTTGCTTGTTTGTTGGGAGAACCGTTTCGGCACGAAAAAAAAAAAAAAACAATGGTAACGTGTTAACGGAtt  <  2:916128/141‑1 (MQ=255)

GCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGA‑AATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATT  >  USA300TCH1516_ALE/1347851‑1348128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`