BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,372,042	A→T	6.1%	V524V (GTA→GTT)	tkt →	Transketolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,372,042	0	A	T	6.1%	403.1 / 10.3	129	V524V (GTA→GTT)	tkt	Transketolase
Reads supporting (aligned to +/- strand): ref base A (56/64); new base T (2/6); total (59/70)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAGA  >  USA300TCH1516_ALE/1371903‑1372179

tAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTAt                                                                                                                                           <  1:440298/141‑1 (MQ=255)

   cTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAATTGTATTTTTAATGGTTCGGCGACCACCGAGATCTACACCTACTTCA‑TAGTATTGACACGTCAAAACTTACCGGTATTAGa                                                                                                                                       <  2:122999/141‑1 (MQ=255)

      gATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATg                                                                                                                                     >  1:717816/1‑141 (MQ=255)

       aTTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAGGTTGGCTGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑t                                                                                                                                   <  2:818727/141‑1 (MQ=255)

       aTTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑t                                                                                                                                   <  2:104292/141‑1 (MQ=255)

       aTTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑t                                                                                                                                   <  3:69765/141‑1 (MQ=255)

          aaGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TAcc                                                                                                                                >  3:176869/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                              >  2:448220/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                              >  1:90826/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                              >  2:954608/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                              >  1:260197/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                              >  1:175534/1‑141 (MQ=255)

             agCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCaga                                                                                                                             <  4:152063/141‑1 (MQ=255)

              gCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCagaa                                                                                                                            <  3:376413/141‑1 (MQ=255)

               ccATTCCAAATGTGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCagaag                                                                                                                           <  3:257927/141‑1 (MQ=255)

               ccATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCCGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCagaag                                                                                                                           <  2:302577/141‑1 (MQ=255)

                  ttCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTCGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATg                                                                                                                        <  3:371479/141‑1 (MQ=255)

                  ttCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATg                                                                                                                        >  2:193743/1‑141 (MQ=255)

                   tCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGTGTAGCATGGGAAGTTGCCTTACAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGt                                                                                                                       <  2:668538/141‑1 (MQ=255)

                   tCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGt                                                                                                                       <  3:8733/141‑1 (MQ=255)

                    ccAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTa                                                                                                                      >  1:167359/1‑141 (MQ=255)

                    ccAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTa                                                                                                                      >  2:418883/1‑141 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAAGAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAg                                                                                                                     <  1:801949/141‑1 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAg                                                                                                                     <  1:820201/141‑1 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAg                                                                                                                     >  3:57991/1‑141 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACAAGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAg                                                                                                                     >  3:16311/1‑141 (MQ=255)

                     cAAATATGAATGATATCCGTCCTGCAGATCGTAATGAAACAAGAGTAGAATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAg                                                                                                                     >  2:138502/1‑141 (MQ=255)

                      aaaTATTAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGCGTAGCATGGGAAGTTGCCTGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGt                                                                                                                    <  2:1037601/141‑1 (MQ=255)

                      aaaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGAAGAGTCACCACTTACTGGT‑GTAGGTGTTACCAGAGGATGTAGt                                                                                                                    >  1:313874/1‑141 (MQ=255)

                       aaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGtt                                                                                                                   >  1:107043/1‑141 (MQ=255)

                       aaTATGAATGTTATCCGTCCGGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGtt                                                                                                                   <  2:846927/141‑1 (MQ=255)

                        atatGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTg                                                                                                                  >  3:58692/1‑141 (MQ=255)

                           tgtattttATCCGTTCTGCTGATGGCAATGAAACAAGAGTAGCAGTGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAATATGTAGTTgaag                                                                                                               <  2:972005/135‑1 (MQ=255)

                                gTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGc                                                                                                          >  3:352576/1‑141 (MQ=255)

                                gTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGc                                                                                                          <  2:496534/141‑1 (MQ=255)

                                 ttATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCg                                                                                                         >  3:291135/1‑141 (MQ=255)

                                    tCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTc                                                                                                      >  2:34650/1‑141 (MQ=255)

                                     ccGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCg                                                                                                     <  2:772524/141‑1 (MQ=255)

                                     ccGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCg                                                                                                     <  2:630443/141‑1 (MQ=255)

                                       gTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGaa                                                                                                   >  2:1002149/1‑141 (MQ=255)

                                               gATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGcc                                                                                           <  1:615401/141‑1 (MQ=255)

                                               gATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGcc                                                                                           >  1:805148/1‑141 (MQ=255)

                                                aTGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAggggtct                                                                                          >  4:329929/1‑136 (MQ=255)

                                                 tGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTCGTTGAAGAAGGCGTTCGAAAAGGTGCCta                                                                                         <  4:45487/141‑1 (MQ=255)

                                                 tGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCta                                                                                         >  2:172394/1‑141 (MQ=255)

                                                 tGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCta                                                                                         >  1:988281/1‑141 (MQ=255)

                                                  ggTAATGAGAAAAGAGTAGCATGGGTAGTTGCCTTAGGATATGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGGTG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAGAGGTGCCttt                                                                                        <  2:116623/141‑3 (MQ=255)

                                                  ggTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCtat                                                                                        <  4:172977/141‑1 (MQ=255)

                                                  ggTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCtat                                                                                        <  2:116688/141‑1 (MQ=255)

                                                     aaTGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTCGAAGAAGGCGTTCGAAAAGGTGCCTATACa                                                                                     <  1:305879/141‑1 (MQ=255)

                                                      aTGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAg                                                                                    >  3:121456/1‑141 (MQ=255)

                                                       tGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGt                                                                                   >  3:234529/1‑141 (MQ=255)

                                                       tGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGt                                                                                   <  2:497754/141‑1 (MQ=255)

                                                         aaaCAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGttt                                                                                 >  2:517569/1‑141 (MQ=255)

                                                           aCAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTAt                                                                               <  1:620342/141‑1 (MQ=255)

                                                            cAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATg                                                                              <  1:566119/141‑1 (MQ=255)

                                                             aaGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATgg                                                                             <  3:53125/141‑1 (MQ=255)

                                                             aaGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATgg                                                                             <  2:332176/141‑1 (MQ=255)

                                                                  tAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTg                                                                        <  2:847009/141‑1 (MQ=255)

                                                                   aGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGa                                                                       >  1:869664/1‑141 (MQ=255)

                                                                    gCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGaa                                                                      >  4:325552/1‑141 (MQ=255)

                                                                    gCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGaa                                                                      >  1:151987/1‑141 (MQ=255)

                                                                      aTGGGAAGTTGCCTTAGACTCTGTATCTGCACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAaga                                                                    <  2:757956/141‑1 (MQ=255)

                                                                             gTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCa                                                             <  3:310806/141‑1 (MQ=255)

                                                                                  cTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAAtt                                                        <  1:170167/141‑1 (MQ=255)

                                                                                   ttAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCAGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTc                                                       <  1:543767/141‑1 (MQ=255)

                                                                                     acaatctgcatccACACCAACTTCATTAGTACTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGAGCCTATACCGTTTATGGCTCTGAAGGGACACCAGAATTACt                                                     <  4:47488/139‑1 (MQ=255)

                                                                                       aatctgaatctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTGTGGCTCTGAAGAGACACCAGAATTCCtat                                                   >  4:220377/1‑141 (MQ=255)

                                                                                           tgaatctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTattatt                                               >  1:34646/1‑141 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                            <  2:415659/141‑1 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGAGG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                            <  1:822624/141‑1 (MQ=255)

                                                                                                  acacCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCa                                        >  1:21362/1‑141 (MQ=255)

                                                                                                     ccTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGt                                     <  1:703636/141‑1 (MQ=255)

                                                                                                      cTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGtt                                    >  1:1035428/1‑141 (MQ=255)

                                                                                                      cTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGtt                                    >  3:345313/1‑141 (MQ=255)

                                                                                                          ttCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGATGAAGAAGGGTTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGa                                <  1:102127/141‑1 (MQ=255)

                                                                                                            cATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAg                              <  1:863665/141‑1 (MQ=255)

                                                                                                             aTTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGt                             >  1:167955/1‑141 (MQ=255)

                                                                                                              ttAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGtt                            <  2:1053160/141‑1 (MQ=255)

                                                                                                               tAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTa                           >  2:146819/1‑141 (MQ=255)

                                                                                                                  tATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTc                        <  2:382859/141‑1 (MQ=255)

                                                                                                                   aTTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCt                       >  1:649378/1‑141 (MQ=255)

                                                                                                                      gACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGc                    >  4:455033/1‑141 (MQ=255)

                                                                                                                      gACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGc                    >  1:370407/1‑141 (MQ=255)

                                                                                                                        cacGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAg                  <  1:952918/141‑1 (MQ=255)

                                                                                                                        cacGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAg                  <  2:717816/141‑1 (MQ=255)

                                                                                                                         acGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGt                 <  1:593083/141‑1 (MQ=255)

                                                                                                                             cAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGaa             <  1:1081062/141‑1 (MQ=255)

                                                                                                                              aaaaCTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAg            >  2:732711/1‑141 (MQ=255)

                                                                                                                              aaaaCTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAg            <  2:956504/141‑1 (MQ=255)

                                                                                                                                  cTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAgctgc        <  4:39952/141‑1 (MQ=255)

                                                                                                                                    tACCTGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTa      <  1:38601/141‑1 (MQ=255)

                                                                                                                                     aCCGGTTTTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaa     <  2:595128/141‑1 (MQ=255)

                                                                                                                                     aCCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaa     <  2:1048362/141‑1 (MQ=255)

                                                                                                                                      ccGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaaa    >  2:971858/1‑141 (MQ=255)

                                                                                                                                      ccGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaaa    <  1:740018/141‑1 (MQ=255)

                                                                                                                                      ccGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaaa    >  3:22259/1‑141 (MQ=255)

                                                                                                                                       cGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAg   <  1:34364/141‑1 (MQ=255)

                                                                                                                                       cGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAg   >  1:191836/1‑141 (MQ=255)

                                                                                                                                        ggTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAGa  >  3:378882/1‑141 (MQ=255)

TAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAGA  >  USA300TCH1516_ALE/1371903‑1372179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`