| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,493,285 | A→T | 5.0% | V2913V (GTT→GTA) | ebh_1 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,493,285 | 0 | A | T | 5.0% | 423.6 / 8.7 | 133 | V2913V (GTT→GTA) | ebh_1 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base A (64/62); new base T (4/3); total (68/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
TTGTTTGCGTGTCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCATTTTGCAACTG > USA300TCH1516_ALE/1493146‑1493425 | ttGTTTGCGTGTCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCaa < 4:191657/141‑1 (MQ=255)ttGTTTGCGTGTCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCaa < 1:74866/141‑1 (MQ=255) tttGCGTGTCAATACTATGCTGTAGTTGATTTTTCGCATTTTCTAAAGGCGATTTATCACCTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGCTTTGACGTATTGTGGCAACAt > 2:762684/1‑141 (MQ=255) cgtatCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGTGCTTTATCGACTGTTAAGCCATTGCGCGCTTGATCAAGTGCTGTTTTAGCAGCATTCGCTGCTGTCACGTTTTGACGAATCGTTGCTACATCTg‑g < 3:372137/137‑1 (MQ=255) tgtCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GAt > 3:416327/1‑141 (MQ=255) gtCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GAtg < 1:747416/141‑1 (MQ=255) tACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAgg > 2:34516/1‑141 (MQ=255) cTATGTTGTAGTTGTTTTTTCGCATTTTCTAAAGGCGCCTTATCGACTGTTAAGCCATTCCGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCt < 3:186986/141‑1 (MQ=255) aTGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCCTTCTCGACGGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTaa > 1:520823/1‑141 (MQ=255) gttgtAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATa > 2:33545/1‑141 (MQ=255) ttgtAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTAGCAACATCTG‑GATGTGAGACTAATac > 4:126956/1‑141 (MQ=255) tttAGTTGATTTTTCGCATGTTCTAAAGGCGCTGTATCGGCTGTTAAGCCATGACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGNTAATaca > 2:813331/3‑141 (MQ=255) tgtAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATaca > 1:981577/1‑141 (MQ=255) gtAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATacat > 3:165848/1‑140 (MQ=255) gtAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATacac < 2:94478/141‑1 (MQ=255) aGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGa < 2:73421/141‑1 (MQ=255) gTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGAt < 3:449186/141‑1 (MQ=255) gTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGAt > 2:673538/1‑141 (MQ=255) ttttCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAAttt > 1:337701/1‑141 (MQ=255) ttCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCt < 3:289538/141‑1 (MQ=255) ttCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCt < 4:232933/141‑1 (MQ=255) tCGCATTTTCTAATGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCtt < 4:116460/141‑1 (MQ=255) tCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTTATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCtt < 4:264327/141‑1 (MQ=255) cGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTg > 1:164531/1‑141 (MQ=255) tttttttAAAGGCGCTTTATCGACAGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGTCGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAAt > 4:163134/2‑141 (MQ=255) aTTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTTTTGAAt > 3:180865/1‑141 (MQ=255) aTTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAAt > 3:180862/1‑141 (MQ=255) cTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAg < 1:403888/141‑1 (MQ=255) cTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAg > 1:409355/1‑141 (MQ=255) cTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAg > 4:109112/1‑141 (MQ=255) tAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTATCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGt < 2:1076197/141‑1 (MQ=255) ggCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTa > 1:67739/1‑141 (MQ=255) ggCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTa > 3:49590/1‑141 (MQ=255) gcgcTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAg < 1:727056/141‑1 (MQ=255) gcTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAgct < 1:804590/141‑1 (MQ=255) gcTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAgct > 1:816874/1‑141 (MQ=255) gcTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAgct > 2:854382/1‑141 (MQ=255) tttATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAgctgc > 2:54197/1‑141 (MQ=255) ttATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAgctgct < 1:86602/141‑1 (MQ=255) tgtCGACTGTTAGGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGctgctg < 2:177048/139‑1 (MQ=255) aTCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGa < 1:141267/141‑1 (MQ=255) gACTGTTAAGCCATTTCGTGCTTGATCAAGTGCTGATTGAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAg < 1:65935/141‑1 (MQ=255) aCTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTGTTGAATTTTAGTTCTAGCTGCTGAAAGt > 2:916043/1‑141 (MQ=255) aaGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCa < 1:885724/141‑1 (MQ=255) gCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCAtt > 1:985730/1‑141 (MQ=255) gCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCAtt > 2:795840/1‑141 (MQ=255) gCCATTACGTGCTTGATCAAGTGCTGACTTAGCGACATTCGCTGCTTTCACGGTTTGACGTATTGTCTCTTCTTCTG‑GTTGTGGGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCAtt > 1:319584/1‑141 (MQ=255) gtaatggCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAt > 3:313415/7‑141 (MQ=255) aCGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgc < 2:566762/141‑1 (MQ=255) aCGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgc > 2:168093/1‑141 (MQ=255) cGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgct > 2:344887/1‑141 (MQ=255) cGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgct < 1:1049035/141‑1 (MQ=255) cGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgct < 3:367443/141‑1 (MQ=255) cGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAAtgct < 2:152050/141‑1 (MQ=255) gCTTGATCAAGTGCTGTTTTAGCAGCATTCGCTGCTGTCACGTTTTGACGAATCGTTGCTACATCTG‑GATGAGATGCTAGTACGCGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTGTCATTAAATgcagca > 1:376142/1‑141 (MQ=255) cTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCaa > 2:402976/1‑141 (MQ=255) ttGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAAt < 4:34725/141‑1 (MQ=255) tGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATa < 2:691562/141‑1 (MQ=255) gATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAg < 3:388357/141‑1 (MQ=255) aGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATgcg < 1:130945/141‑1 (MQ=255) gTGCTGATTTGGCGGCATTGGCGGTTGTCTCATTGGAATGTTTGGTCGCAAGATCTGTGTTGT‑AGTCGAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATgcgc < 1:762684/141‑1 (MQ=255) gTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATgcgc > 1:497707/1‑141 (MQ=255) gTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATgcgc > 1:1084571/1‑141 (MQ=255) gCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCtt > 2:1082552/1‑141 (MQ=255) cTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTg < 4:313856/141‑1 (MQ=255) tGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGt < 4:49932/141‑1 (MQ=255) aTTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCa > 2:185650/1‑141 (MQ=255) tAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATAAATTTCTTGAATTCTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATAc > 2:138796/1‑141 (MQ=255) aGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATAcc < 2:613081/141‑1 (MQ=255) aGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATAcc > 1:877994/1‑141 (MQ=255) gCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCa < 2:981577/141‑1 (MQ=255) cGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAg > 2:872963/1‑141 (MQ=255) cGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAg > 2:873064/1‑141 (MQ=255) cGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAg > 4:267224/1‑141 (MQ=255) cGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACAAGATCAATTTCTTGAATTTTCGTTCTAGCTGCTGAAAGTTTTTCATTACATGCTGCAATAGCTGCGCTTGTCATACCAg > 1:593636/1‑141 (MQ=255) cGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGGGAGGCTAATACACGATCAAGTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTCTTTCATTAAATGCTGCAATAGATGCGCCTGTCATACCAg > 2:666836/1‑141 (MQ=255) ggCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGt > 1:300623/1‑141 (MQ=255) aTTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGt < 2:144904/141‑1 (MQ=255) aTTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GACGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGt < 1:854382/141‑1 (MQ=255) tCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTAc < 1:737826/141‑1 (MQ=255) ctgctgTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCg < 2:27453/141‑1 (MQ=255) tgctgTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTTAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGt < 3:129989/141‑1 (MQ=255) tgctgTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGt > 2:491653/1‑141 (MQ=255) tgctgTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGt < 3:64749/141‑1 (MQ=255) tgTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTgg < 3:17620/141‑1 (MQ=255) tCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCt > 3:76813/1‑141 (MQ=255) aCGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGa > 1:821163/1‑141 (MQ=255) cGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCGGTCGTACTCGTTGGCTGAt > 1:483644/1‑141 (MQ=255) cGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGAt < 2:728423/141‑1 (MQ=255) tttGACGAATCGTTGCTACATCTG‑GATGAGATGCTAGTACGCGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTGTCATTAAATGCAGCAACAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCaa < 4:257487/141‑1 (MQ=255) gACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGGTGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAatat > 1:101328/1‑141 (MQ=255) aCGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCCGTCGTACTCGTTGGCTGATCAATATc > 2:303012/1‑141 (MQ=255) cGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCa > 4:71782/1‑141 (MQ=255) cGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCa > 2:59704/1‑141 (MQ=255) gTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCAt < 1:898636/141‑1 (MQ=255) tATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCAtt > 1:184670/1‑141 (MQ=255) aTTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCAttt < 2:374388/141‑1 (MQ=255) gCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCATTTTGCAAc < 1:448031/141‑1 (MQ=255) cAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCATTTTGCAACt > 2:1056148/1‑141 (MQ=255) aaCATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCATTTTGCAACTg < 2:365964/141‑1 (MQ=255) | TTGTTTGCGTGTCAATACTATGTTGTAGTTGATTTTTCGCATTTTCTAAAGGCGCTTTATCGACTGTTAAGCCATTACGTGCTTGATCAAGTGCTGATTTAGCGGCATTCGCTGCTGTCACGTTTTGACGTATTGTCGCAACATCTG‑GATGTGAGGCTAATACACGATCAATTTCTTGAATTTTAGTTCTAGCTGCTGAAAGTTTTTCATTAAATGCTGCAATAGATGCGCTTGTCATACCAGTCGTACTCGTTGGCTGATCAATATCATTTTGCAACTG > USA300TCH1516_ALE/1493146‑1493425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |