BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,083,000	G→T	12.1%	S580* (TCA→TAA)	USA300HOU_RS10525 ←	65 kDa membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,083,000	0	G	T	12.1%	337.9 / 7.6	127	S580* (TCA→TAA)	USA300HOU_RS10525	65 kDa membrane protein
Reads supporting (aligned to +/- strand): ref base G (52/56); new base T (17/2); total (69/58)
Fisher's exact test for biased strand distribution p-value = 8.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GCTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTT  >  USA300TCH1516_ALE/2082861‑2083138

gCTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGa                                                                                                                                           >  1:1071201/1‑141 (MQ=255)

gCTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGa                                                                                                                                           >  3:306729/1‑141 (MQ=255)

gCTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGa                                                                                                                                           >  1:417124/1‑141 (MQ=255)

gCTGATTTAAGTTTGATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTGTTTTGa                                                                                                                                           <  4:374940/141‑1 (MQ=255)

 cTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TATTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGAt                                                                                                                                          >  1:786119/1‑141 (MQ=255)

    aTTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTa                                                                                                                                       >  3:215299/1‑141 (MQ=255)

     tttAAGTTTTATATTTAAATCATTAATTGTAA‑TTTTGATTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTTTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAg                                                                                                                                      <  2:105921/141‑1 (MQ=255)

         aGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAgtgtt                                                                                                                                  >  2:528122/1‑140 (MQ=255)

          tttttATACTTAAATCATTAATTGTAA‑TTTTGTTTGCATTATAGAACAAAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                 <  1:871847/140‑1 (MQ=255)

          gTTTTATNCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                 <  2:813753/141‑1 (MQ=255)

          gTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                 >  1:654756/1‑141 (MQ=255)

           ttttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAtt                                                                                                                                >  4:22003/1‑141 (MQ=255)

             ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTATTTTTTTTTTGATTTAGTGTATTg                                                                                                                               >  1:433052/1‑141 (MQ=255)

             ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGTTTTCTTGAATTGt                                                                                                                              >  1:321427/1‑141 (MQ=255)

             ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGt                                                                                                                              >  1:154988/1‑141 (MQ=255)

               ataCTTAAATCATTAGTTGTAA‑TATTGCTTGCGTTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTAtttttt                                                                                                                            >  4:69109/1‑141 (MQ=255)

               ataCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGttt                                                                                                                            >  1:677669/1‑141 (MQ=255)

               ataCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGttt                                                                                                                            <  1:528769/141‑1 (MQ=255)

                taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                           >  3:114656/1‑141 (MQ=255)

                taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                           >  1:416617/1‑141 (MQ=255)

                taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                           >  3:98378/1‑141 (MQ=255)

                taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                           >  3:98395/1‑141 (MQ=255)

                 aCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTa                                                                                                                          >  1:310483/1‑141 (MQ=255)

                 aCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTa                                                                                                                          >  1:584152/1‑141 (MQ=255)

                   ttAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACa                                                                                                                        >  1:858256/1‑141 (MQ=255)

                     aactCATTATTTGTTG‑TTTTGCTTTCATTATAGTACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGATTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatc                                                                                                                      <  1:143162/138‑1 (MQ=255)

                     aaaTCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatc                                                                                                                      <  2:618321/141‑1 (MQ=255)

                        tCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaat                                                                                                                   >  2:609646/1‑141 (MQ=255)

                          atcatttGTAA‑TTTTGCTTTCATTATATAACATAAATTCATTTAAAAATTTGTTTTAAAATTTTTTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatat                                                                                                                 <  1:306305/136‑1 (MQ=255)

                          aTTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTTAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatat                                                                                                                 <  4:169591/141‑1 (MQ=255)

                          aTTAATTGTAA‑TTTTGCTTGCATCATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTTTTGTTTTATttcattat                                                                                                                 >  2:194547/1‑141 (MQ=255)

                           ttAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTATATTCTTTTACatcaatatc                                                                                                                >  4:40895/1‑141 (MQ=255)

                             aaTTGTAATTTTTGCTTTGATTATAGAACACATATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatca                                                                                                               <  2:909392/141‑1 (MQ=255)

                              aTTTTAA‑TTTTGCTTGCATTATAGAACATAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTATATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatcaat                                                                                                             <  4:333709/141‑1 (MQ=255)

                              aTTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatcaat                                                                                                             >  1:886851/1‑141 (MQ=255)

                              aTTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatcaat                                                                                                             <  2:462461/141‑1 (MQ=255)

                                 gTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTATTACATCAATATAAATCtt                                                                                                          >  2:99707/1‑141 (MQ=255)

                                  tAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttt                                                                                                         >  4:309889/1‑141 (MQ=255)

                                   aa‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCtttt                                                                                                        >  3:204869/1‑141 (MQ=255)

                                    a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTATATTGTTTTACATCAATATCAATCttttt                                                                                                       >  2:804961/1‑141 (MQ=255)

                                    a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttttt                                                                                                       >  1:366983/1‑141 (MQ=255)

                                    a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttttt                                                                                                       <  3:324735/141‑1 (MQ=255)

                                       tttGCTTGCATTATAGAACACAAATTTATTTGAAACTATGTTTTAAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTaa                                                                                                     <  4:174806/141‑1 (MQ=255)

                                       tttGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTaa                                                                                                     <  2:137617/141‑1 (MQ=255)

                                              gcATTATAGAACACAANTTCATTTGAAACNATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCttt                                                                                              >  1:975582/1‑141 (MQ=255)

                                                 ttATAGAACACAAATTCATTTGAAACTTTTTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGc                                                                                           <  2:758404/141‑1 (MQ=255)

                                                    taGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACt                                                                                        >  1:844247/1‑141 (MQ=255)

                                                     aGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTTGTGTATTGTTTTACATCAATATCAATTTTTTTAATATCTTTGGCACta                                                                                       >  2:931240/1‑141 (MQ=255)

                                                      gAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTAATGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaa                                                                                      <  1:502302/141‑1 (MQ=255)

                                                       aaCACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTTTTTTACATTAATATTAATTTTTTTAATATTTTTGGTATtaaa                                                                                     >  2:529577/1‑141 (MQ=255)

                                                        acacAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCTATATCAATCTTTTTAATATCTTTGGCACtaaat                                                                                    <  2:393438/141‑1 (MQ=255)

                                                         cacaAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTATTTTATTGTTTTACATCAATATCAATCTTTTTAATATTTTTTGCTCtatata                                                                                   >  2:502548/1‑141 (MQ=255)

                                                         cacaAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaata                                                                                   >  1:549102/1‑141 (MQ=255)

                                                           caAATTCATTTGAATCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaataaa                                                                                 <  3:430407/141‑1 (MQ=255)

                                                           caAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaataaa                                                                                 <  1:950355/141‑1 (MQ=255)

                                                           caAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaataaa                                                                                 <  3:430397/141‑1 (MQ=255)

                                                                tCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAgg                                                                            >  1:398022/1‑141 (MQ=255)

                                                                 cATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGgt                                                                           >  1:426119/1‑141 (MQ=255)

                                                                   tttGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGgtgt                                                                         >  2:1001039/1‑141 (MQ=255)

                                                                   tttGAAACTATGTTTTCAAATTTATTTATTGTTACTAGGAATTTGACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTATTATCTTTGGCACTAAATAAATTAGgtgt                                                                         >  1:106069/1‑141 (MQ=255)

                                                                        aaCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTTTATAGa                                                                    >  1:52887/1‑141 (MQ=255)

                                                                        aaCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGa                                                                    >  1:946438/1‑141 (MQ=255)

                                                                          ttATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATg                                                                  <  2:710793/140‑1 (MQ=255)

                                                                           tATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTATTTTTTTTTTTTTTTTCTGTCTTGCTTTAAATTCACCTCAATATCTTTAATCTCTTTGGCAATCAATCAATTAGGTGTCCAGATg                                                                  >  1:539755/1‑141 (MQ=255)

                                                                           tATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTTTTTTACATCAATATCAATTTTTTTAATATCTTTGGTATTAAATAAATTATGTGTTAATATGt                                                                 >  2:979985/1‑141 (MQ=255)

                                                                             tGTTTTCCAATTTATTTATTGTTATTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTATTATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                               <  4:276204/141‑1 (MQ=255)

                                                                             tGTTTTCAAATTTATTTATTGTTATTTGTAATTTCACATTATTAGATTTATTTA‑TTTTTTTGTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                               <  1:1032776/141‑1 (MQ=255)

                                                                             tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTATTTTTTTGTTTTATATCAATATTACTCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTATAGTTGttt                                                               >  2:180971/1‑139 (MQ=255)

                                                                             tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                               >  1:601398/1‑141 (MQ=255)

                                                                             tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                               <  2:290426/141‑1 (MQ=255)

                                                                             tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                               <  1:211674/141‑1 (MQ=255)

                                                                                  tCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGAttt                                                          <  2:867703/141‑1 (MQ=255)

                                                                                  tCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGAttt                                                          <  4:306729/141‑1 (MQ=255)

                                                                                      atttatttATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTaaa                                                      <  3:22003/141‑1 (MQ=255)

                                                                                        ttatttatTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATCTTTTTAATATTTTTGGCACTAAATAACTTAGGTTTAAAGATGTCTGATTTTatttt                                                    >  4:422294/1‑137 (MQ=255)

                                                                                           tttattGTTACTGGGAATTTCATATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTctc                                                 <  4:73684/141‑3 (MQ=255)

                                                                                             tattGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCacta                                               >  3:393998/1‑141 (MQ=255)

                                                                                               ttGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCactact                                             >  4:56785/1‑141 (MQ=255)

                                                                                                 gTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTg                                           <  4:411560/141‑1 (MQ=255)

                                                                                                     ttGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttc                                       <  2:607150/140‑1 (MQ=255)

                                                                                                     cTGAGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTGTTTTACATCAATATCAATTATTTTACTATCTTTGGCAATCAATAAATTAGTTGTAACGATTTTTGTTTTTAAATTCACTACTTGtttt                                       >  4:390316/1‑140 (MQ=255)

                                                                                                      tGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttct                                      <  1:1003294/141‑1 (MQ=255)

                                                                                                       tgtaaTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttctt                                     <  4:225318/138‑1 (MQ=255)

                                                                                                       gggAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttctt                                     <  3:59110/141‑1 (MQ=255)

                                                                                                        ggAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAATTTAGGTGTAAATATGTCTGTTTTTAAATTCACTACTTGtttcttt                                    >  2:70277/1‑141 (MQ=255)

                                                                                                          aaTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTcc                                  <  1:100432/141‑1 (MQ=255)

                                                                                                           aTTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAATGATGTCTGATTTTAAATTCACTACTTGTTTCTTTcct                                 >  2:1053304/1‑140 (MQ=255)

                                                                                                           aTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCa                                 >  1:306666/1‑141 (MQ=255)

                                                                                                                        aGATTTATTTA‑TTTTTTTTTTTTTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAAtata                    >  2:292527/1‑141 (MQ=255)

                                                                                                                         tatttattta‑tttTTTTTTGATTTAGTTTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATAc                   <  2:170576/140‑1 (MQ=255)

                                                                                                                            ttattta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGt                <  1:791071/141‑1 (MQ=255)

                                                                                                                             tattta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTTGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATGTTTGAAATATACTGta               <  2:436844/141‑1 (MQ=255)

                                                                                                                               ttta‑tttTTTTTTTTTTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTTGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGtata             >  2:327437/1‑141 (MQ=255)

                                                                                                                               ttta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGtata             <  2:393838/141‑1 (MQ=255)

                                                                                                                                  a‑tttTTTTTTTTTTTTTTTTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTTTAAAGATGTCTGATTTTAAATTTTTTTCTTTTTTTTTTTCATTTTTTAATTATACTTTATAttt          >  2:301483/1‑141 (MQ=255)

                                                                                                                                  a‑tttTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGTACTAAATAAATTAGGTGTAAAGATGTCTTATTTTAAATTCAATACTTGTTTCTTTCCATTTTTGGGATATAAGGTTTAttt          <  2:694948/141‑1 (MQ=255)

                                                                                                                                  a‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAttt          <  1:277847/141‑1 (MQ=255)

                                                                                                                                   ttttttttttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGCCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAttt          <  2:568087/140‑1 (MQ=255)

                                                                                                                                    tttttttttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAtttt         <  2:196089/141‑1 (MQ=255)

                                                                                                                                           ttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGtt  >  3:406895/1‑141 (MQ=255)

GCTGATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTT  >  USA300TCH1516_ALE/2082861‑2083138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`