BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,083,005	A→T	9.9%	T578T (ACT→ACA)	USA300HOU_RS10525 ←	65 kDa membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,083,005	0	A	T	9.9%	313.7 / 4.2	124	T578T (ACT→ACA)	USA300HOU_RS10525	65 kDa membrane protein
Reads supporting (aligned to +/- strand): ref base A (50/58); new base T (13/2); total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.58e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTTTCGCA  >  USA300TCH1516_ALE/2082865‑2083143

aTTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTa                                                                                                                                            >  3:215299/1‑141 (MQ=255)

 tttAAGTTTTATATTTAAATCATTAATTGTAA‑TTTTGATTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTTTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAg                                                                                                                                           <  2:105921/141‑1 (MQ=255)

     aGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAgtgtt                                                                                                                                       >  2:528122/1‑140 (MQ=255)

      tttttATACTTAAATCATTAATTGTAA‑TTTTGTTTGCATTATAGAACAAAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                      <  1:871847/140‑1 (MQ=255)

      gTTTTATNCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                      <  2:813753/141‑1 (MQ=255)

      gTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAt                                                                                                                                      >  1:654756/1‑141 (MQ=255)

       ttttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAtt                                                                                                                                     >  4:22003/1‑141 (MQ=255)

       ttttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAAAACAAAAATTTATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTAtt                                                                                                                                     <  1:625482/141‑1 (MQ=255)

         ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTATTTTTTTTTTGATTTAGTGTATTg                                                                                                                                    >  1:433052/1‑141 (MQ=255)

         ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGTTTTCTTGAATTGt                                                                                                                                   >  1:321427/1‑141 (MQ=255)

         ttATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGt                                                                                                                                   >  1:154988/1‑141 (MQ=255)

           ataCTTAAATCATTAGTTGTAA‑TATTGCTTGCGTTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTAtttttt                                                                                                                                 >  4:69109/1‑141 (MQ=255)

           ataCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGttt                                                                                                                                 >  1:808950/1‑141 (MQ=255)

           ataCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGttt                                                                                                                                 >  1:677669/1‑141 (MQ=255)

            taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                                >  3:114656/1‑141 (MQ=255)

            taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                                >  1:416272/1‑141 (MQ=255)

            taCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGtttt                                                                                                                                >  3:98378/1‑141 (MQ=255)

             aCTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTa                                                                                                                               >  1:310483/1‑141 (MQ=255)

               ttAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACa                                                                                                                             >  1:858256/1‑141 (MQ=255)

                 aactCATTATTTGTTG‑TTTTGCTTTCATTATAGTACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGATTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatc                                                                                                                           <  1:143162/138‑1 (MQ=255)

                 aaaTCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatc                                                                                                                           <  2:618321/141‑1 (MQ=255)

                    tCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaat                                                                                                                        >  2:609646/1‑141 (MQ=255)

                      atcatttGTAA‑TTTTGCTTTCATTATATAACATAAATTCATTTAAAAATTTGTTTTAAAATTTTTTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatat                                                                                                                      <  1:306305/136‑1 (MQ=255)

                      aTTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTTAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatat                                                                                                                      <  4:169591/141‑1 (MQ=255)

                      aTTAATTGTAA‑TTTTGCTTGCATCATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTTTTGTTTTATttcattat                                                                                                                      >  2:194547/1‑141 (MQ=255)

                       ttAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTATATTCTTTTACatcaatatc                                                                                                                     >  4:40895/1‑141 (MQ=255)

                         aaTTGTAATTTTTGCTTTGATTATAGAACACATATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatca                                                                                                                    <  2:909392/141‑1 (MQ=255)

                          aTTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACatcaatatcaat                                                                                                                  <  2:462461/141‑1 (MQ=255)

                             gTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTATTACATCAATATAAATCtt                                                                                                               >  2:99707/1‑141 (MQ=255)

                              tAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttt                                                                                                              >  4:309889/1‑141 (MQ=255)

                               aa‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCtttt                                                                                                             >  3:204869/1‑141 (MQ=255)

                                a‑TTTTGCTTTCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttttt                                                                                                            <  3:79620/141‑1 (MQ=255)

                                a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTATATTGTTTTACATCAATATCAATCttttt                                                                                                            >  2:804961/1‑141 (MQ=255)

                                a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttttt                                                                                                            <  3:324735/141‑1 (MQ=255)

                                a‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCttttt                                                                                                            >  1:366983/1‑141 (MQ=255)

                                   tttGCTTGCATTATAGAACACAAATTTATTTGAAACTATGTTTTAAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTaa                                                                                                          <  4:174806/141‑1 (MQ=255)

                                          gcATTATAGAACACAANTTCATTTGAAACNATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCttt                                                                                                   >  1:975582/1‑141 (MQ=255)

                                             ttATAGAACACAAATTCATTTGAAACTTTTTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGc                                                                                                <  2:758404/141‑1 (MQ=255)

                                             ttATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTTGc                                                                                                >  2:25209/1‑141 (MQ=255)

                                                taGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACt                                                                                             >  1:844247/1‑141 (MQ=255)

                                                 aGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTTGTGTATTGTTTTACATCAATATCAATTTTTTTAATATCTTTGGCACta                                                                                            >  2:931240/1‑141 (MQ=255)

                                                 aGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACta                                                                                            <  1:888693/141‑1 (MQ=255)

                                                 aGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACta                                                                                            >  2:371017/1‑141 (MQ=255)

                                                  gAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTAATGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaa                                                                                           <  1:502302/141‑1 (MQ=255)

                                                   aaCACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTTTTTTACATTAATATTAATTTTTTTAATATTTTTGGTATtaaa                                                                                          >  2:529577/1‑141 (MQ=255)

                                                    acacAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCTATATCAATCTTTTTAATATCTTTGGCACtaaat                                                                                         <  2:393438/141‑1 (MQ=255)

                                                     cacaAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTATTTTATTGTTTTACATCAATATCAATCTTTTTAATATTTTTTGCTCtatata                                                                                        >  2:502548/1‑141 (MQ=255)

                                                     cacaAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaata                                                                                        >  1:549102/1‑141 (MQ=255)

                                                       caAATTCATTTGAATCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaataaa                                                                                      <  3:430407/141‑1 (MQ=255)

                                                       caAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACtaaataaa                                                                                      <  1:950355/141‑1 (MQ=255)

                                                            tCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAgg                                                                                 >  1:398022/1‑141 (MQ=255)

                                                            tCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAgg                                                                                 >  1:464019/1‑141 (MQ=255)

                                                            tCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAgg                                                                                 <  1:545141/141‑1 (MQ=255)

                                                             cATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGgt                                                                                >  1:426119/1‑141 (MQ=255)

                                                               tttGAAACTATGTTTTCAAATTTATTTATTGTTACTAGGAATTTGACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTATTATCTTTGGCACTAAATAAATTAGgtgt                                                                              >  1:106069/1‑141 (MQ=255)

                                                                    aaCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTTTATAGa                                                                         >  1:52887/1‑141 (MQ=255)

                                                                    aaCTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGa                                                                         >  1:946438/1‑141 (MQ=255)

                                                                      ttATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATg                                                                       <  2:710793/140‑1 (MQ=255)

                                                                       tATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTATTTTTTTTTTTTTTTTCTGTCTTGCTTTAAATTCACCTCAATATCTTTAATCTCTTTGGCAATCAATCAATTAGGTGTCCAGATg                                                                       >  1:539755/1‑141 (MQ=255)

                                                                       tATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTTTTTTACATCAATATCAATTTTTTTAATATCTTTGGTATTAAATAAATTATGTGTTAATATGt                                                                      >  2:979985/1‑141 (MQ=255)

                                                                         tGTTTTCCAATTTATTTATTGTTATTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTATTATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                                    <  4:276204/141‑1 (MQ=255)

                                                                         tGTTTTCAAATTTATTTATTGTTATTTGTAATTTCACATTATTAGATTTATTTA‑TTTTTTTGTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                                    <  1:1032776/141‑1 (MQ=255)

                                                                         tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTATTTTTTTGTTTTATATCAATATTACTCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTATAGTTGttt                                                                    >  2:180971/1‑139 (MQ=255)

                                                                         tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                                    >  1:601398/1‑141 (MQ=255)

                                                                         tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                                    <  2:290426/141‑1 (MQ=255)

                                                                         tGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCt                                                                    <  1:211674/141‑1 (MQ=255)

                                                                              tCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGAttt                                                               <  4:306729/141‑1 (MQ=255)

                                                                              tCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGAttt                                                               <  2:867703/141‑1 (MQ=255)

                                                                                  atttatttATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTaaa                                                           <  3:22003/141‑1 (MQ=255)

                                                                                       tttattGTTACTGGGCTTTTCTTATTATTAGATTTATTTA‑TTTTTTTTTGATTTTGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAGATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCac                                                      <  4:38129/141‑1 (MQ=255)

                                                                                       tttattGTTACTGGGAATTTCATATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTctc                                                      <  4:73684/141‑3 (MQ=255)

                                                                                         tattGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCacta                                                    >  4:448236/1‑141 (MQ=255)

                                                                                         tattGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCacta                                                    >  3:393998/1‑141 (MQ=255)

                                                                                           ttGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCactact                                                  >  4:56785/1‑141 (MQ=255)

                                                                                             gTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTg                                                <  4:411560/141‑1 (MQ=255)

                                                                                                 ttGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttc                                            <  2:607150/140‑1 (MQ=255)

                                                                                                 cTGAGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTTTATTGTTTTACATCAATATCAATTATTTTACTATCTTTGGCAATCAATAAATTAGTTGTAACGATTTTTGTTTTTAAATTCACTACTTGtttt                                            >  4:390316/1‑140 (MQ=255)

                                                                                                  tGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttct                                           <  1:1003294/141‑1 (MQ=255)

                                                                                                   gggAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGtttctt                                          <  3:59110/141‑1 (MQ=255)

                                                                                                    ggAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAATTTAGGTGTAAATATGTCTGTTTTTAAATTCACTACTTGtttcttt                                         >  2:70277/1‑141 (MQ=255)

                                                                                                      aaTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTcc                                       <  1:100432/141‑1 (MQ=255)

                                                                                                       aTTTCACATTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAATGATGTCTGATTTTAAATTCACTACTTGTTTCTTTcct                                      >  2:1053304/1‑140 (MQ=255)

                                                                                                       aTTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCa                                      >  1:306666/1‑141 (MQ=255)

                                                                                                          tCACATTATTAGATTTATTTA‑‑TTTTTTTTGTTTTTGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTTGCACTAATTAAATTAGGTGTAACGATGTCTGATTTTAAATTCACTCCTTGTTTCTTTCCAtttt                                  <  2:158531/141‑1 (MQ=255)

                                                                                                             caTTATTAGATTTATTTA‑TTTTTTTTTTTTTTTTTGTATTGTTTTACATCAATATCAATTTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTTACTACTTGTTTCTTTTCAtttttt                                >  2:1026066/1‑140 (MQ=255)

                                                                                                                    aGATTTATTTA‑TTTTTTTTTTTTTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAAtata                         >  2:292527/1‑141 (MQ=255)

                                                                                                                     tatttattta‑tttTTTTTTGATTTAGTTTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATAc                        <  2:170576/140‑1 (MQ=255)

                                                                                                                        ttattta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGt                     <  4:19193/141‑1 (MQ=255)

                                                                                                                         tattta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTTGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATGTTTGAAATATACTGta                    <  2:436844/141‑1 (MQ=255)

                                                                                                                           ttta‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGtata                  <  2:393838/141‑1 (MQ=255)

                                                                                                                              a‑tttTTTTTTTTTTTTTTTTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTTTAAAGATGTCTGATTTTAAATTTTTTTCTTTTTTTTTTTCATTTTTTAATTATACTTTATAttt               >  2:301483/1‑141 (MQ=255)

                                                                                                                              a‑tttTTTTTTTATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGTACTAAATAAATTAGGTGTAAAGATGTCTTATTTTAAATTCAATACTTGTTTCTTTCCATTTTTGGGATATAAGGTTTAttt               <  2:694948/141‑1 (MQ=255)

                                                                                                                              a‑tttTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAttt               <  1:277847/141‑1 (MQ=255)

                                                                                                                               ttttttttttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGCCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAttt               <  2:568087/140‑1 (MQ=255)

                                                                                                                                tttttttttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATAtttt              >  4:278088/1‑141 (MQ=255)

                                                                                                                                     ttttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTg         >  3:397621/1‑141 (MQ=255)

                                                                                                                                       ttGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGtt       >  3:406895/1‑141 (MQ=255)

                                                                                                                                         taTTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTAGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTTTc     <  1:139421/140‑1 (MQ=255)

                                                                                                                                          aTTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTTTCg    <  2:106069/141‑1 (MQ=255)

                                                                                                                                            ttAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTTTCGCa  <  2:677669/141‑1 (MQ=255)

ATTTAAGTTTTATACTTAAATCATTAATTGTAA‑TTTTGCTTGCATTATAGAACACAAATTCATTTGAAACTATGTTTTCAAATTTATTTATTGTTACTGGGAATTTCACATTATTAGATTTATTTA‑TTTTTTTTTGATTTAGTGTATTGTTTTACATCAATATCAATCTTTTTAATATCTTTGGCACTAAATAAATTAGGTGTAAAGATGTCTGATTTTAAATTCACTACTTGTTTCTTTCCATTTTTGAAATATACTGTATATTTTGCTTGTTTCGCA  >  USA300TCH1516_ALE/2082865‑2083143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`