BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,120,673	A→G	6.1%	V34V (GTT→GTC)	USA300HOU_RS10785 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,120,673	0	A	G	6.1%	340.8 / 9.8	115	V34V (GTT→GTC)	USA300HOU_RS10785	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (62/46); new base G (5/2); total (67/48)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 120 total aligned reads displayed.

TCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATT  >  USA300TCH1516_ALE/2120534‑2120812

tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa                                                                                                                                             <  1:852966/141‑1 (MQ=255)

 cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTTGaacaac                                                                                                                                            <  3:402656/141‑1 (MQ=255)

 cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaac                                                                                                                                            >  1:714974/1‑141 (MQ=255)

     aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                                                                                                                        <  1:281820/141‑1 (MQ=255)

          gTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGAc                                                                                                                                   <  3:286398/141‑1 (MQ=255)

           tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                                  >  2:1065026/1‑141 (MQ=255)

           tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                                  >  3:273068/1‑141 (MQ=255)

            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                                                                                                                 >  2:705909/1‑139 (MQ=255)

            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                 >  1:663286/1‑141 (MQ=255)

                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                             >  2:538008/1‑141 (MQ=255)

                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                             >  1:644608/1‑141 (MQ=255)

                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                            >  1:734959/1‑140 (MQ=255)

                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                            >  1:526314/1‑140 (MQ=255)

                 tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                                                                            <  2:1003342/141‑1 (MQ=255)

                     cTGTGTTATTTTCAATCCAATCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                                        <  3:179267/141‑1 (MQ=255)

                       gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                                                                      <  1:455632/141‑2 (MQ=255)

                       gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                                                                                                                      >  2:635553/1‑141 (MQ=255)

                          ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                                                                   <  2:629719/141‑1 (MQ=255)

                            aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                                                                 <  1:299598/141‑1 (MQ=255)

                             ttttCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTa                                                                                                                <  4:322472/141‑1 (MQ=255)

                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                            >  1:372840/1‑141 (MQ=255)

                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                            <  1:309741/141‑1 (MQ=255)

                                  aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                                                                           <  2:82870/141‑1 (MQ=255)

                                   aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                                                                          <  1:846708/141‑1 (MQ=255)

                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                         >  1:992887/1‑141 (MQ=255)

                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                         >  1:475000/1‑141 (MQ=255)

                                        ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                                                                     <  1:641314/141‑1 (MQ=255)

                                         tCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑a                                                                                                   <  3:281012/141‑1 (MQ=255)

                                         tCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑a                                                                                                   >  4:147125/1‑141 (MQ=255)

                                            tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑aaaa                                                                                                >  2:1046637/1‑141 (MQ=255)

                                              aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATc                                                                                              <  2:730749/141‑1 (MQ=255)

                                                cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCaa                                                                                            >  1:432525/1‑141 (MQ=255)

                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                        >  1:940092/1‑141 (MQ=255)

                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                        >  2:101574/1‑141 (MQ=255)

                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                        >  2:944847/1‑141 (MQ=255)

                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                        >  1:219361/1‑141 (MQ=255)

                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                        >  1:1050983/1‑141 (MQ=255)

                                                      gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCAt                                                                                      >  3:271611/1‑141 (MQ=255)

                                                      gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCAt                                                                                      <  1:929619/141‑1 (MQ=255)

                                                            aaaTATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAAATCAAAGCCATCTACa                                                                                 >  1:897473/1‑141 (MQ=255)

                                                              atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACatta                                                                              <  1:460266/141‑1 (MQ=255)

                                                               tatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaa                                                                             <  1:797050/141‑1 (MQ=255)

                                                                  cTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaatta                                                                          >  4:190496/1‑141 (MQ=255)

                                                                  cTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaatta                                                                          <  4:228471/141‑1 (MQ=255)

                                                                     aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTAttt                                                                       >  2:1058932/1‑141 (MQ=255)

                                                                      aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTAtttt                                                                      <  1:576778/141‑1 (MQ=255)

                                                                          gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                                  >  4:128022/1‑141 (MQ=255)

                                                                          gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                                  >  2:409349/1‑141 (MQ=255)

                                                                             tataGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACAt                                                               <  1:439432/141‑1 (MQ=255)

                                                                                 gATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGt                                                           <  4:264319/141‑1 (MQ=255)

                                                                                     gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       <  1:617052/141‑1 (MQ=255)

                                                                                      ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc                                                      >  2:60905/1‑141 (MQ=255)

                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCTTTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCACAGTCTTTTACATTAATTATTTTTCGACATACGGCACCCg                                                     <  4:174304/141‑1 (MQ=255)

                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:927714/1‑141 (MQ=255)

                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:883004/1‑141 (MQ=255)

                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:625438/1‑141 (MQ=255)

                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                    >  2:723512/1‑141 (MQ=255)

                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                    >  2:723508/1‑141 (MQ=255)

                                                                                         tcgtcaGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGtt                                                   >  1:22892/4‑141 (MQ=255)

                                                                                          catcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGttt                                                  >  4:421057/1‑141 (MQ=255)

                                                                                             caGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGt                                               <  1:34641/141‑1 (MQ=255)

                                                                                              aGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCTTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTa                                              >  1:340122/1‑141 (MQ=255)

                                                                                               gAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTaa                                             <  2:379579/141‑1 (MQ=255)

                                                                                                 aaCTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATa                                           <  4:248214/141‑1 (MQ=255)

                                                                                                    tagtagCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACAt                                        >  1:715115/1‑141 (MQ=255)

                                                                                                    tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       <  1:1074934/126‑1 (MQ=255)

                                                                                                    tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       >  2:1074934/1‑126 (MQ=255)

                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       >  3:57508/1‑141 (MQ=255)

                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       <  1:923004/141‑1 (MQ=255)

                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       >  2:993461/1‑141 (MQ=255)

                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       >  1:1014721/1‑141 (MQ=255)

                                                                                                      gTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTg                                      >  2:176986/1‑141 (MQ=255)

                                                                                                        aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                                    >  3:427037/1‑141 (MQ=255)

                                                                                                        aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                                    >  1:26639/1‑141 (MQ=255)

                                                                                                             gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                               >  1:572862/1‑141 (MQ=255)

                                                                                                               cAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcc                             >  2:400888/1‑141 (MQ=255)

                                                                                                                aaTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcct                            >  2:780143/1‑141 (MQ=255)

                                                                                                                 aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                           <  3:403180/141‑1 (MQ=255)

                                                                                                                 aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATAAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                           <  1:590673/141‑1 (MQ=255)

                                                                                                                  ttGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctcc                          <  1:635701/141‑1 (MQ=255)

                                                                                                                   tGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTcctcct                         <  2:205492/141‑1 (MQ=255)

                                                                                                                    gAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCtt                        >  2:114196/1‑141 (MQ=255)

                                                                                                                     aaTTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTg                       >  1:1084372/1‑141 (MQ=255)

                                                                                                                       tttCCTCCCTAGTCTAGAACAACCATTTCGACGCAATACGCTCGCTTCTTAACTCTTAATTG‑AAAAGCAAAGCCATGTACCTTAGTTCTTTTTCGCCATTCGTCACCCGTTTCGTAACGCATTGTTCTCGTCCTCCTTGTc                     <  2:777534/141‑1 (MQ=255)

                                                                                                                        ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGGCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa                    <  1:842338/141‑1 (MQ=255)

                                                                                                                        ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa                    <  3:124859/141‑1 (MQ=255)

                                                                                                                          ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTGATACATTGTTCTCGTCCTCCTTGTCAtt                  >  1:755978/1‑141 (MQ=255)

                                                                                                                          ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAtt                  >  1:996421/1‑141 (MQ=255)

                                                                                                                              cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AACATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTAGTGTAGTACAGTGTTCCTGACGTCTTTGTCATTATCt              >  2:271327/1‑141 (MQ=255)

                                                                                                                              cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt              <  1:766597/141‑1 (MQ=255)

                                                                                                                              cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt              >  4:290744/1‑141 (MQ=255)

                                                                                                                               aTGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑CAAATCAAAGCCATCTACATTAATTATTTTTCGACATACTTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCta             <  2:754503/141‑1 (MQ=255)

                                                                                                                                tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat            >  4:229346/1‑141 (MQ=255)

                                                                                                                                tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat            >  1:861688/1‑141 (MQ=255)

                                                                                                                                    cTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCATCCTTGTCATTATCTATAGCt        <  3:238133/141‑1 (MQ=255)

                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATNCATNGTTCTCGTCCTCCTTNTCATTATCTATAGCTAg      <  1:927714/141‑1 (MQ=255)

                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg      <  1:249101/141‑1 (MQ=255)

                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg      <  1:700825/141‑1 (MQ=255)

                                                                                                                                          caacCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt  >  1:21411/1‑141 (MQ=255)

                                                                                                                                          caacCATNTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt  <  2:816252/141‑1 (MQ=255)

TCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATT  >  USA300TCH1516_ALE/2120534‑2120812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`