Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,176,264 A→G 5.1% intergenic (+26/+5269) ilvA → / ← USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,176,2640AG 5.1% 408.7 / 7.1 135intergenic (+26/+5269)ilvA/USA300TCH1516_02069L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (71/56);  new base G (4/3);  total (75/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATAAAA  >  USA300TCH1516_ALE/2176125‑2176402
                                                                                                                                            |                                                                                                                                           
aTTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAAt                                                                                                                                            >  2:110547/1‑141 (MQ=255)
 ttCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAAtt                                                                                                                                           >  3:145241/1‑141 (MQ=255)
      cTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGAttt                                                                                                                                      <  2:171047/141‑1 (MQ=255)
             aTCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑g                                                                                                                              >  1:617276/1‑141 (MQ=255)
             aTCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑g                                                                                                                              >  3:100194/1‑141 (MQ=255)
                atgatgATTTAATACAACTCAAACAACGGGTAGAACATTTCGATGCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑Gaaa                                                                                                                           >  2:594736/1‑141 (MQ=255)
                  gatgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAG‑CAGTCATAAGATGATTTCAATTA‑GAAatc                                                                                                                         <  4:34317/141‑1 (MQ=38)
                    tgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatcat                                                                                                                       >  3:309868/1‑141 (MQ=255)
                    tgatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatcat                                                                                                                       <  2:332190/141‑1 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatcatc                                                                                                                      >  3:406147/1‑141 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatcatc                                                                                                                      >  2:510057/1‑141 (MQ=255)
                     gatTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatcatc                                                                                                                      >  2:416897/1‑141 (MQ=255)
                         tAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTGAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTAt                                                                                                                  >  1:853946/1‑141 (MQ=255)
                          aaTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATg                                                                                                                 <  4:192681/141‑1 (MQ=255)
                          aaTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATg                                                                                                                 <  1:637285/141‑1 (MQ=255)
                           aTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAGTTGATTTATAATT‑GAAATCATCTTATGa                                                                                                                >  3:404528/1‑141 (MQ=255)
                           aTACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGa                                                                                                                >  1:932694/1‑141 (MQ=255)
                            caCAACTCCAACAACCTGTAAATCATTTCGATCCTTCCAATAATTATATTAATGAAAATAAGATGTGATATTCATTGTTAATTTAACACATACTAAGAACAT‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGAc                                                                                                               <  2:1019040/140‑1 (MQ=255)
                            atcgttacAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGAc                                                                                                               <  1:178671/134‑1 (MQ=255)
                              cAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAAGCAGTCATAAGATGATTTC‑AATTAGAAATCAATTTATGACt                                                                                                              <  3:418999/141‑1 (MQ=25)
                              cAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTg                                                                                                             >  4:61660/1‑141 (MQ=255)
                                aCTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCt                                                                                                           >  1:493904/1‑141 (MQ=255)
                                 cTCACACAACGTGTAAATCGTTACGATCCTTCCAATATTAATATAACAAAAACTAAGATGTTATAATCATAGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTACTAATT‑GACATAATCTAATgcaaactt                                                                                                          >  2:432112/1‑134 (MQ=255)
                                 cTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCtt                                                                                                          >  2:63177/1‑141 (MQ=255)
                                     aacaacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTtta                                                                                                      >  3:443365/1‑141 (MQ=255)
                                       caacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTttatt                                                                                                    >  2:327841/1‑141 (MQ=255)
                                        aacGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTttatta                                                                                                   >  2:482814/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  1:917604/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  4:145175/1‑141 (MQ=255)
                                         acGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTtattat                                                                                                  >  2:105053/1‑141 (MQ=255)
                                          cGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAGATGATTTC‑AATTAGAAATCAATTTATGACTGTttttcttacta                                                                                                 <  1:304750/141‑8 (MQ=21)
                                          cGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATtata                                                                                                 >  2:55904/1‑141 (MQ=255)
                                             gtAAATCGTTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  1:860097/1‑141 (MQ=255)
                                             gtAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  2:93627/1‑141 (MQ=255)
                                             gtAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACtt                                                                                              >  1:178711/1‑141 (MQ=255)
                                              tAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACttt                                                                                             <  2:1051845/141‑1 (MQ=255)
                                              tAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACttt                                                                                             <  2:344375/141‑1 (MQ=255)
                                               aaaTCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTa                                                                                            >  2:77098/1‑141 (MQ=255)
                                               aaaTCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTa                                                                                            >  2:15660/1‑141 (MQ=255)
                                                     tttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtc                                                                                      <  4:446626/141‑1 (MQ=255)
                                                     tttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtc                                                                                      <  1:12964/141‑1 (MQ=255)
                                                      ttCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtct                                                                                     >  1:394398/1‑141 (MQ=255)
                                                       tCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtctt                                                                                    >  2:988276/1‑140 (MQ=255)
                                                       tCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTtctc                                                                                    <  1:1032266/141‑1 (MQ=255)
                                                         gATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAatc                                                                                                                         <  2:829411/102‑1 (MQ=255)
                                                              ttCCAATAAATATATTAATGAAAATAAGATGTGCTCTTCATTGTCAATTTAACACATAATACGAAAAA‑CAGTTATAAATATACTTCCAATT‑GAAATCACCGTATTACTCCTTTTTTTTCAACTTTACAATTGGCGTTTCGt                                                                             >  4:358906/1‑141 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGa                                                                                                                                         <  2:747123/81‑1 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGa                                                                                                                                         >  1:747123/1‑81 (MQ=255)
                                                               tCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTc                                                                            <  1:628611/141‑1 (MQ=255)
                                                                 cAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAg                                                                          <  4:312304/141‑1 (MQ=255)
                                                                   atatTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGAt                                                                        >  4:230486/1‑141 (MQ=255)
                                                                     atTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTc                                                                      <  1:29008/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    <  4:114762/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    <  2:1011380/141‑1 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaa                                                                    >  2:261458/1‑141 (MQ=255)
                                                                       ttATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑AAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTTTAGTTTAGAAAGATTCaa                                                                    >  4:244354/1‑141 (MQ=255)
                                                                        tataTTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCaaa                                                                   >  3:125068/1‑141 (MQ=255)
                                                                                tGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCa                                                           <  1:326538/141‑1 (MQ=255)
                                                                                     aTAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCg                                                      >  2:549347/1‑141 (MQ=255)
                                                                                       aaGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGACATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGcc                                                    >  3:376904/1‑141 (MQ=255)
                                                                                        aGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCa                                                   >  1:153076/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCCGATTCAAACGTTTTCACTTCGCCAAg                                                 >  2:627859/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAg                                                 >  3:178783/1‑141 (MQ=255)
                                                                                          aTGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAg                                                 >  1:527439/1‑141 (MQ=255)
                                                                                          aTGTTATATACATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTCCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCTGATTCAAACGTTTTCACTTCGCCAAg                                                 >  2:233805/1‑141 (MQ=255)
                                                                                           tGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGc                                                >  2:282748/1‑141 (MQ=255)
                                                                                           tGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGc                                                >  4:209599/1‑141 (MQ=255)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGGAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTTCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTCGTCACTTCGCCCAGccg                                              <  2:384668/141‑2 (MQ=39)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCa                                              <  4:114882/141‑1 (MQ=255)
                                                                                             ttATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCa                                              <  4:114870/141‑1 (MQ=255)
                                                                                               atatTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtc                                            >  4:274079/1‑141 (MQ=255)
                                                                                                 atTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtctt                                          <  4:46507/141‑1 (MQ=255)
                                                                                                    cATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCAtctttct                                       <  1:295787/141‑1 (MQ=255)
                                                                                                      ttGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATctttcttt                                     >  3:125870/1‑141 (MQ=255)
                                                                                                       tGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTtg                                    <  2:999764/141‑1 (MQ=255)
                                                                                                              ttAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑TAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGc                             <  3:268213/141‑1 (MQ=255)
                                                                                                              ttAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGc                             >  3:126397/1‑141 (MQ=255)
                                                                                                               tAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCt                            <  3:118092/141‑1 (MQ=255)
                                                                                                                  cacaTAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCtttt                         >  3:54076/1‑141 (MQ=255)
                                                                                                                      tAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTAttt                     <  2:1050861/141‑1 (MQ=255)
                                                                                                                       ataataaaaag‑CAGTCATAAGATGATTTC‑AATTAGAAATCAATTTATGACTGTTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTAtttt                    >  1:655153/7‑141 (MQ=18)
                                                                                                                        gTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTg                   <  4:309868/141‑1 (MQ=255)
                                                                                                                         tAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGa                  >  1:139373/1‑141 (MQ=255)
                                                                                                                            gAAACA‑CGGTCATATATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTGCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  2:289723/141‑1 (MQ=255)
                                                                                                                            gAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  1:987705/141‑1 (MQ=255)
                                                                                                                            aaaaaa‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGt               <  2:738287/140‑1 (MQ=255)
                                                                                                                             aaaag‑CAGTCATAAGATGATTTCAATTA‑GAAATCAGTTTATGACTGTTTTTTATTATACTTTACATTGCTCGTTTCGTCAGCTTCAAACGTTTTCACTTCGCCAAGCCATCTGTCTTTGTGTTTGCTTTTATATTGACGtt              >  1:934597/1‑141 (MQ=21)
                                                                                                                             aaaag‑CAGTCATAAGATGATTTCAATTA‑GAAATCAATTTATGACTGTTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              >  2:808841/1‑141 (MQ=18)
                                                                                                                             aaaaa‑CAGTCATAAATTGATTTCTAATT‑GTAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCGGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              <  4:422144/141‑1 (MQ=255)
                                                                                                                             aaaaa‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGtt              >  2:898603/1‑141 (MQ=255)
                                                                                                                              aaaa‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTCATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGttt             >  2:802570/1‑141 (MQ=255)
                                                                                                                                   cAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGa         >  2:230034/1‑141 (MQ=255)
                                                                                                                                   cAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGa         >  4:35544/1‑141 (MQ=255)
                                                                                                                                    aGTCATAATTTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGTCGTATTAGAc        <  2:13511/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  1:321819/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  3:230486/141‑1 (MQ=255)
                                                                                                                                    aGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGAc        <  1:740268/141‑1 (MQ=255)
                                                                                                                                      tCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTTACGTTTTaaaaaa      <  4:60136/141‑6 (MQ=255)
                                                                                                                                      tCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTGGACAt      <  3:203799/141‑1 (MQ=255)
                                                                                                                                          aaaTTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATaaaa  <  3:299942/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGTGTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTGTTAATTTAACACATAGTAAGAAAAA‑CAGTCATAAATTGATTTCTAATT‑GAAATCATCTTATGACTGCTTTTTATTATACTTTACATTTCTCGTTTCGTCAGATTCAAACGTTTTCACTTCGCCAAGCCATCTTTCTTTGTGTTTGCTTTTATTTTGACGTTTTAGACATAAAA  >  USA300TCH1516_ALE/2176125‑2176402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑