BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	419,854	G→A	9.6%	intergenic (+72/+194)	rpsR → / ← USA300HOU_RS01950	30S ribosomal protein S18/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	419,854	0	G	A	9.6%	376.8 / 3.9	134	intergenic (+72/+194)	rpsR/USA300HOU_RS01950	30S ribosomal protein S18/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (74/44); new base A (2/14); total (76/58)
Fisher's exact test for biased strand distribution p-value = 2.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATGTTGACTACAGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCTTG  >  USA300TCH1516_ALE/419714‑419992

aTGTTGACTACAGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAgg                                                                                                                                            >  1:243232/1‑141 (MQ=255)

     gACTACAGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCaaa                                                                                                                                       >  2:216801/1‑141 (MQ=255)

          cAGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGTCAAAAAAgg                                                                                                                                  <  1:956571/141‑1 (MQ=255)

           aGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTCTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGga                                                                                                                                 >  3:299451/1‑141 (MQ=255)

            gCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTTGGGTATAGAAAAAGGGCAAAAAAGgat                                                                                                                                >  4:18799/1‑141 (MQ=255)

                 cAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGAtgt                                                                                                                           <  1:877716/141‑1 (MQ=255)

                 cAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGAtgt                                                                                                                           <  1:396717/141‑1 (MQ=255)

                  aaaCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATTTAATATATTAT‑AACCCTCGTAGGCATAGGCTTATGGGGGA‑TTTTTTTATGTTTGGGATATAGAAAAGGGACAGAAAAgggcaaaaa                                                                                                                           <  1:727981/141‑8 (MQ=255)

                  aaaCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATTTAATATATTAT‑AACCCTCGTAGGCATAGGCTTA‑TGGGGGATTTTTTTATGTTTGGGATATAGAAAAGGGACAGAAAAgggcaaaaa                                                                                                                           <  1:726149/141‑8 (MQ=255)

                    aCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGaa                                                                                                                        >  2:275959/1‑141 (MQ=255)

                    aCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGaa                                                                                                                        >  4:279899/1‑141 (MQ=255)

                    aCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGaa                                                                                                                        <  1:894999/141‑1 (MQ=255)

                    aCGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGaa                                                                                                                        >  1:728783/1‑141 (MQ=255)

                       ttCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGt                                                                                                                     <  1:844281/141‑1 (MQ=255)

                          aagacaaatGGAATTATTACCATATGTTAAAGAAGAACAATAATATATAATATATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAAAAAAAAAAAAAAAAAGGATGATGTGAATGtttt                                                                                                                  <  2:519261/134‑1 (MQ=255)

                           cGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTtg                                                                                                                 >  2:311897/1‑141 (MQ=255)

                           cGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTtg                                                                                                                 >  1:554329/1‑141 (MQ=255)

                            gTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTtgt                                                                                                                >  1:334027/1‑141 (MQ=255)

                             tCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGCATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTtgtg                                                                                                               >  2:421302/1‑141 (MQ=255)

                                tatGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTc                                                                                                            <  2:435245/141‑1 (MQ=255)

                                tatGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTc                                                                                                            >  4:48907/1‑141 (MQ=255)

                                     cATTATTACCATATGTTAAAGAAGAACAGTAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAAt                                                                                                       <  3:404185/141‑1 (MQ=255)

                                     cATTATTACCATATGTTAAAGAAGAAAAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAAAGAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAAt                                                                                                       <  4:167801/141‑1 (MQ=255)

                                     cATGATTACCATATGTTAAATAAGAACAATAATATATAATTTATGTGCAAACTCCGTAGGCATAGTCTTA‑CGTGGC‑TTTTTGTGT‑TTTGGTGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAAt                                                                                                       >  2:542492/1‑141 (MQ=255)

                                        tattaCCATATGTTAAAGAAGAACAAGAAAATGTAATGTATTGTCAAACCCCGCAGGCATAGGCTTA‑CGGTGC‑TTTTTGTGT‑TTTGGGGTATAAAAAAAAAAAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTg                                                                                                    <  2:929757/141‑1 (MQ=255)

                                          ttaCCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTGCGGAATTTGca                                                                                                  >  2:495094/1‑141 (MQ=255)

                                          ttaCAATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGca                                                                                                  <  2:203690/141‑1 (MQ=255)

                                            aCCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGcaca                                                                                                >  2:843373/1‑141 (MQ=255)

                                                tatGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGa                                                                                            >  4:405010/1‑141 (MQ=255)

                                                 atGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGat                                                                                           >  2:1019193/1‑141 (MQ=255)

                                                   gTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAAAAAAAAAAAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGatat                                                                                         <  4:302694/141‑1 (MQ=255)

                                                    ttAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATg                                                                                        >  1:653248/1‑141 (MQ=255)

                                                    ttAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATg                                                                                        >  1:458333/1‑141 (MQ=255)

                                                      aaaaaaaaACAAAAAAAAAGAAGTTAATGACAAACCCCGTAGGCGTAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAAAAAAAAAAAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGtt                                                                                      <  2:482286/141‑1 (MQ=255)

                                                      aaaGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGtt                                                                                      <  3:126827/141‑1 (MQ=255)

                                                      aaaGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGtt                                                                                      <  3:141757/141‑1 (MQ=255)

                                                      aaaGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGtt                                                                                      >  1:697850/1‑141 (MQ=255)

                                                       aagaagAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGttt                                                                                     >  1:527424/1‑141 (MQ=255)

                                                           agaaAAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGA‑TTTGGGGTATAGAAAAAGAGAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTatat                                                                                 <  2:884941/141‑1 (MQ=255)

                                                             aaCAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTg                                                                               >  2:834274/1‑141 (MQ=255)

                                                               cAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCa                                                                             >  1:521761/1‑141 (MQ=255)

                                                                aataatATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAACAACGGAAGATGAGACACTTGTGTCTTCGGCGTTTGCCCAAAGCTATGTTTATATTGCaa                                                                            >  3:16240/1‑141 (MQ=255)

                                                                aataatATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCaa                                                                            >  1:382864/1‑141 (MQ=255)

                                                                   aataTATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCaaaaa                                                                         >  1:147771/1‑141 (MQ=255)

                                                                   aataTATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCaaaaa                                                                         >  2:696146/1‑141 (MQ=255)

                                                                      atataATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAaataa                                                                      >  3:430553/1‑141 (MQ=255)

                                                                       aaaaaTTTATTGTCAAACCCCGTAGGCATAGGCTTA‑AGGGGC‑TTTTTGTGT‑TTTGGGGTATAAAAAAAAAAAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAaataat                                                                     <  2:61279/138‑1 (MQ=255)

                                                                        ataATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACaaa                                                                                              <  4:361177/115‑1 (MQ=255)

                                                                        ataATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAataata                                                                    <  3:115869/141‑1 (MQ=255)

                                                                         taATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAatat                                                                   <  1:638412/141‑1 (MQ=255)

                                                                          aaTTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATg                                                                  <  1:670222/141‑1 (MQ=255)

                                                                               aTTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGACAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCACAAATAATATGAAttt                                                             >  1:837292/1‑141 (MQ=255)

                                                                                  gTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGa                                                          >  1:276923/1‑141 (MQ=255)

                                                                                   tCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGAt                                                         >  1:775377/1‑141 (MQ=255)

                                                                                    aaaaCCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGAAAAAAAAAAAAAAATAATATGAATTTAGATg                                                        <  4:407644/140‑1 (MQ=255)

                                                                                     aaaCCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGc                                                       >  2:264233/1‑141 (MQ=255)

                                                                                       aCCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCAt                                                     <  2:259673/141‑1 (MQ=255)

                                                                                         cccGTAGGCATAGGCTTA‑CGGGGC‑TTTTTTTGT‑TTTTGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATaa                                                   >  4:210837/1‑141 (MQ=255)

                                                                                          ccGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGC‑AAAATAATATGAATTTAGATGCATaaaa                                                 >  4:36567/1‑141 (MQ=255)

                                                                                            gTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATaaaaa                                                >  2:709842/1‑141 (MQ=255)

                                                                                            gTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATaaaaa                                                >  3:24307/1‑141 (MQ=255)

                                                                                            gTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATaaaaa                                                >  2:141055/1‑141 (MQ=255)

                                                                                              aGGCATAGGCTTA‑CGGGGC‑TTTTTTTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATaaaaaaa                                              >  4:27225/1‑141 (MQ=255)

                                                                                              aGGCATAGGCTTA‑CGGGGC‑ATTTTGGGT‑TTTGGGGTATCGAAAAAGAGCAAAAAAGGACGATGTGAATGTTTTGTGTTCGGAATTGGCACAACGATATGTTTATATTCCAAAAATAGTATGAATACAGATGCATcaaaaaa                                              >  1:590766/1‑141 (MQ=255)

                                                                                                gCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGa                                            >  1:160866/1‑141 (MQ=255)

                                                                                                      gCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACg                                      <  3:189711/141‑1 (MQ=255)

                                                                                                        ttA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTATAAATTGAAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCa                                    <  2:334027/141‑1 (MQ=255)

                                                                                                          a‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCAtt                                  >  2:216350/1‑141 (MQ=255)

                                                                                                            cGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCAttt                                 >  2:325858/1‑141 (MQ=255)

                                                                                                              gggC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGAAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTta                               <  2:558386/141‑1 (MQ=255)

                                                                                                               agC‑TTTATGTGT‑TTAGGGGTATAGAAAAAAAAAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATAATGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTtaa                              <  2:85286/140‑1 (MQ=255)

                                                                                                                 c‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTtaaat                            <  1:297072/141‑1 (MQ=255)

                                                                                                                   tttttGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAGAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTtaaata                           <  1:1004675/141‑1 (MQ=255)

                                                                                                                   tttttGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTtaaata                           >  4:259064/1‑141 (MQ=255)

                                                                                                                    ttttGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTtaaataa                          <  1:249306/141‑1 (MQ=255)

                                                                                                                    ttttGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATAAAAAAAAAAAAATAAAAAAAGAACTACGCATTTtaaataa                          <  2:243232/141‑1 (MQ=255)

                                                                                                                      ttGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATaaaa                        >  1:101868/1‑141 (MQ=255)

                                                                                                                        gtgt‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATg                      >  1:241221/1‑141 (MQ=255)

                                                                                                                          gt‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCa                    >  1:19117/1‑141 (MQ=255)

                                                                                                                          gt‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCa                    >  3:204520/1‑141 (MQ=255)

                                                                                                                          gt‑TTTGGGGTATAGAAAAAGAGAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCa                    <  2:435673/141‑1 (MQ=255)

                                                                                                                           t‑tttGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCAt                   <  2:687064/141‑1 (MQ=255)

                                                                                                                             tttGTGTTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCCTTTTAAATAAAATGCATa                  >  4:76216/1‑141 (MQ=255)

                                                                                                                              tttttttataGAAAAAGGGCAAAAAAGGTTGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTTTTTTTCATTTATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAg                 >  2:937886/7‑141 (MQ=255)

                                                                                                                               ttttttataGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGc                >  4:198443/6‑141 (MQ=255)

                                                                                                                               tGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGc                >  4:399975/1‑141 (MQ=255)

                                                                                                                               tGGGGTATAGAAAAAGGGAAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAACATGCATAGc                <  1:954599/141‑1 (MQ=255)

                                                                                                                                ggggTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGct               <  2:45502/141‑1 (MQ=255)

                                                                                                                                 gggTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCGAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGctc              <  4:139848/141‑1 (MQ=255)

                                                                                                                                    tataGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCtcttc           <  1:598234/141‑1 (MQ=255)

                                                                                                                                     ataGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCtcttct          <  3:401636/141‑1 (MQ=255)

                                                                                                                                     ataGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCtcttct          <  1:1019193/141‑1 (MQ=255)

                                                                                                                                        aaaaaaGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCtttt       <  2:551789/140‑1 (MQ=255)

                                                                                                                                         aaaaaGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAGAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCttttt      >  3:349703/1‑141 (MQ=255)

                                                                                                                                          aaaaGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTc     >  2:1030273/1‑141 (MQ=255)

                                                                                                                                           aaaGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGGTGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCt    <  2:358364/141‑1 (MQ=255)

                                                                                                                                           aaaGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCt    >  2:918698/1‑141 (MQ=255)

                                                                                                                                           aaaGGGCAAAAAAGGATGATGTACATGTTTGGTTGTCGGAATTTGTATAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCt    <  3:16189/141‑1 (MQ=255)

                                                                                                                                             aGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCTTg  <  3:336126/141‑1 (MQ=255)

                                                                                                                                             aGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTATATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCTTg  >  2:1002410/1‑141 (MQ=255)

ATGTTGACTACAGCTATCAAACGTTCTCGTCATATGGCATTATTACCATATGTTAAAGAAGAACAATAATATATAATTTATTGTCAAACCCCGTAGGCATAGGCTTA‑CGGGGC‑TTTTTGTGT‑TTTGGGGTATAGAAAAAGGGCAAAAAAGGATGATGTGAATGTTTTGTGTTCGGAATTTGCACAAAGATATGTTTATATTGCAAAAATAATATGAATTTAGATGCATAAAAAAAGAACTACGCATTTTAAATAAAATGCATAGCTCTTCTTTTTCTTG  >  USA300TCH1516_ALE/419714‑419992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`